1,720,981 research outputs found
Frequency of nonsense mutations (amber and ochre) induced by UV light in the ura3 gene in Saccharomyces cerevisiae.
No full textPROPRANOLOL, ATENOLOL, AND TRIFLUOPERAZINE REDUCE THE SPONTANEOUS OCCURRENCE OF MEIOTIC DIPLOID PRODUCTS IN SACCHAROMYCES-CEREVISIAE
No full textOn a conjecture by Sylwia Cichacz and Tomasz Hinc, and a related problem
No full textA r-magic rectangle set MRS Gamma(a, b; c) is a collection of c arrays of size a x b whose entries are the elements of an abelian group r of order abc, each one appearing once and in a unique array in such a way that the sum of the entries of each row is equal to a constant omega is an element of r and the sum of the entries of each column is equal to a constant delta is an element of r. In this paper we provide new evidences for the validity of a conjecture proposed by Sylwia Cichacz and Tomasz Hinc on the existence of an MRS Gamma(a, b; c). We also generalize this problem describing constructions of r-magic rectangle sets whose elements are partially filled arrays. (c) 2025 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Flocculation expression in a strain of wine yeast Saccharomyces cerevisiae. Ann. Microbiol. Enz. 47, 53-62.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Preparation of high molecular weight plant DNA and its use for artificial chromosome construction
No full textThe availability of a substantial amount of high molecular weight DNA is an essential prerequisite for the construction of yeast artificial chromosome (YAC) libraries. Parameters concerning protoplast isolation and DNA extraction have been systematically analyzed. Conditions have been established for the obtainment of high molecular weight DNA from Arabidopsis thaliana and Nicotiana plumbaginifolia protoplasts either embedded in agarose plugs or in liquid suspension. Restriction fragments were obtained by partial and total digestion with different endonucleases, and separated by pulsed-field gel electrophoresis. Ligation of partially EcoRI-digested DNA (range 30-300 kbp) followed by transformation of yeast spheroplasts gave rise to YACs with an average size of 60 kbp. The introduction of a DNA size-selection step before ligation led to production of YACs in the range of 100-200 kbp. Clones of up to 460 kbp were obtained by blunt-end ligation of pre-selected unrestricted DNA. © 1991 Spr..
Isolation of large DNA fragments of Arabidopsis thaliana and construction of YAC-based megaclones
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