19 research outputs found
Uji Ketahanan Galur-galur Harapan Padi Terhadap Penyakit Hawar Daun Bakteri (Xanthomonas Oryzae Pv. Oryzae) Ras III, IV, Dan VIII
Get PDFResistance Test of Promising Rice Lines Against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) Race III, IV, and VIII. Siti Yuriyah, Dwinita W. Utami, and Ida Hanarida. Development of new superior rice varieties resistant to bacterial leaf blight (BLB) has been conducted through utilizing of a wide rice germplasm, from crossing between IR64 and Oryza rufipogon. The aim of this study is to get promising rice lines that resistant to BLB race III, IV, and VIII. The experiments were conducted at greenhouse and Laboratory of Molecular Biology, ICABIOGRAD Bogor, using of 13 promising rice lines that have different levels of resistance to inoculum from pure cultures of BLB race III, IV and VIII. Of these 13 rice lines, six lines showed resistance to race III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111-BC-PIR7, Bio129-BC-WBC, Bio148-Mamol, and Bio154-Mamol-Dro), one line showed resistance to race IV (Bio154-Mamol-Dro), and one line showed resistance to race VIII (Bio5-AC-Blas/BLB-03), with severity rate 1.8 to 8.1%. Of these improve lines Bio5-AC-Blas/BLB-3 and Bio 111-BCPir- 7, were released as new rice varieties, namely Inpari HDB and Inpari Blas, respectively
Uji Ketahanan Galur-galur Harapan Padi terhadap Penyakit Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) Ras III, IV, dan VIII
No full text<p>Resistance Test of Promising Rice Lines Against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) Race III, IV, and VIII. Siti Yuriyah, Dwinita W. Utami, and Ida Hanarida. Development of new superior rice varieties resistant to bacterial leaf blight (BLB) has been conducted through utilizing of a wide rice germplasm, from crossing between IR64 and Oryza rufipogon. The aim of this study is to get promising rice lines that resistant to BLB race III, IV, and VIII. The experiments were conducted at greenhouse and Laboratory of Molecular Biology, ICABIOGRAD Bogor, using of 13 promising rice lines that have different levels of resistance to inoculum from pure cultures of BLB race III, IV and VIII. Of these 13 rice lines, six lines showed resistance to race III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111-BC-PIR7, Bio129-BC-WBC, Bio148-Mamol, and Bio154-Mamol-Dro), one line showed resistance to race IV (Bio154-Mamol-Dro), and one line showed resistance to race VIII (Bio5-AC-Blas/BLB-03), with severity rate 1.8 to 8.1%. Of these improve lines Bio5-AC-Blas/BLB-3 and Bio 111-BCPir- 7, were released as new rice varieties, namely Inpari HDB and Inpari Blas, respectively.</p><p> </p><p><strong>Abstrak</strong></p><p>Perakitan varietas padi unggul baru tahan terhadap hawar daun bakteri (HDB) terus dilakukan melalui pemanfaatan sumber daya genetik yang luas. Saat ini telah dirakit galur-galur unggul yang berasal dari persilangan IR64 dan padi liar Rupifogon. Penelitian ini bertujuan untuk mengevaluasi ketahanan galur harapan padi terhadap penyakit HDB dari Ras III, IV, dan VIII. Percobaan dilakukan di rumah kaca dan Laboratorium Kelti Biologi Molekuler, BB Biogen, Bogor. Materi genetik yang digunakan adalah 13 galur harapan padi dan biakan murni dari Ras III, IV, dan VIII. Metode inokulasi dengan pengguntingan dan skoring ketahanan dilakukan sesuai dengan sistem penilaian dari Yashitola et al. (1997). Dari 13 galur padi harapan yang diuji, diperoleh enam galur tahan terhadap Ras III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111- BC-PIR7, Bio129-BC-WBC, Bio148-Mamol-Dro, dan Bio154-Mamol-Dro), satu galur tahan terhadap Ras IV (Bio154-Mamol-Dro) dan satu galur tahan terhadap Ras VIII (Bio5-AC-Blas/BLB-03), dengan tingkat keparahan sebesar 1,8-8,1%. Dalam perkembangannya, dua dari galur harapan, yaitu galur Bio5-AC-Blas/BLB-03 dan Bio111-BC-Pir-7 disetujui untuk dilepas sebagai varietas Inpari HDB dan Inpari Blas.</p></jats:p
Faktor Virulensi AvrBs3/PthA pada Ras III, Ras IV, Ras VIII, dan IXO93-068 Patogen Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae)
No full text<p>AvrBs3/PthA Virulence Factor of Bacterial Leaf Blight<br />Race III, Race IV, Race VIII, and IXO93-068. Dwinita W.<br />Utami, Triny S. Kadir, and Siti Yuriyah. Bacterial leaf<br />blight (BLB) is an important disease of rice and present<br />throughout many of the rice-growing regions in the world,<br />also in Indonesia. Xanthomonas oryzae pv. oryzae (Xoo) is<br />the causal agent and a member of the Protebacteria and like<br />many other this phyllum have a type III secretion system for<br />protein virulence effector (PVE) released on their pathogenicity<br />system. Commonly, PVE in Xanthomonas sp., is<br />coded by AvrBs3/PthA family gene. This research was<br />coducted to identify the virulence factor of AvrBs3/PthA on<br />dominant Indonesian BLB isolates (Race III, Race IV, Ras<br />VIII, and IXO93-068). This objective was obtained by<br />sequence analysis through designed markers for members<br />of the virulence factor AvrBs3/PthA gene family (PthXo4,<br />avrXa7#38, PthXoS and avrXa7sacB50). Results gave information<br />that RaceIII is a dependent elicitor race due to no<br />PVE transcript formed and intraceluler protein target with<br />RLL type on NLS (nuclear localization signal). RaceIV and<br />RaceVIII are the virulent race which PVE active formed with<br />intraceluler protein target and have the RLL and RLLP type<br />for the NLS signal. While isolate IXO93-068 is a virulen<br />isolate that active formed a PVE but the extraceluler protein<br />target is due to no type of NLS. Based on cluster analysis,<br />Race VIII has a genetic distance closely to PthXoS and<br />avrXa7sacB50.</p></jats:p
Pencarian Alel untuk Identifikasi Gen Ketahanan Penyakit Hawar Daun Bakteri, Xa7 pada Plasma Nutfah Padi Lokal Indonesia
No full text<p>Allele Mining of Bacterial Blight Resistance Gene, Xa7 on<br />Indonesian Local Rice Germplasm. Dwinita W. Utami,<br />Endang M. Septiningsih, Trini S. Kadir, Fatimah, and Siti<br />Yuriyah. The abundance of novel genetic variation existing<br />in germplasm collections is the foundation for variety<br />improvement in plant breeding program. Nevertheless,<br />studies on Indonesian genetic diversity rice germplasm<br />using molecular markers are still poorly. Recent advances in<br />utilizing of simple sequence repeat (SSR) in QTL mapping<br />and whole rice genome sequences were positive support on<br />genetic diversity of rice germplasm research. Based on these<br />advance technology, we developed the research to discover<br />new alleles at important gene loci that can be used for rice<br />improvement. This approach is recognized as allele mining<br />technology. On this study the target genes for allele mining<br />research is the resistance gene for bacterial leaf blight<br />pathogen, Xa7. This point was introduced by identify the<br />genetic diversity of 96 accessions Indonesian local rice<br />germplasm. The Xa7 allele mining was done by SNP (single<br />nucleotide polymorphism) designing primers based on DNA<br />sequence around the gene target. The significant LD map<br />was detected by association mapping between phenotype<br />and SNP genotyping data of the selected germplasm which<br />having superior performance on BLB resistance and<br />representing on genetic diversity clustering. The results<br />shown that Xa7 allele variation were found in Parekaligolara<br />(Indica, 15141), and Gajah Mada (Indica, 5856), which<br />resistant to BLB races IV and VIII on generative stage and<br />field condition. The significant Xa7-SNP8 and Xa7-SNP11<br />markers were associating with the LD map position of Xa7<br />gene on 28, 05-28,1Mb of chromosome 6 in rice genome.</p></jats:p
Genetic Diversity of Indonesian Bacterial Leaf Blight Isolate (Xanthomonas oryzae pv. oryzae) Core Collection based on the VNTR and avrXa7 Molecular Markers
Get PDFBacterial leaf blight (BLB) is one of the major diseases in rice caused by Xanthomonas oryzae pv. oryzae. This study aimed to identify and analyze the genetic diversity of 18 BLB isolates that consist of 7 races and 11 haplotypes from various locations in Indonesia. The genetic diversity analysis was conducted on the basis of the VNTR (Variable Number of Tandem Repeat) markers and the avrxa7 gene marker. The banding pattern of the amplification product was made into binary data as input for the construction of a dendogram. Based on the dendogram, three X. oryzae pv. oryzae genotype groups with different virulence levels were formed. The VII (IXO80_021) race of X. oryzae pv. oryzae genotype group I and the VIII-A (IXO 80_024) race of genotype group II were avirulent, whereas the races and haplotypes of genotype group III were virulent
Identifikasi Gen Ketahanan terhadap (Xanthomonasoryzae pv. Oryzae), penyebab Penyakit Hawar Daun Bakteri padaPlasma Nutfah Padi Lokal Sulawesi Selatan
No full textOne of limiting factor in rice production especially in South Sulawesi was the bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. Oryzae (Xoo). In South Sulawesi, it had been reported that 58% Xoo of race III were dominant in Bone, Sopeng, Wajo, Sidrap, Barru, Pangkep. Meanwhile, 23% of race IV and 19% of race VIII were dominant in Maros. The effort that considered the most effective to control BLB disease was the planting of resistant variety. The rice variety assembling by using the resistance genes from various cultivars gave the opportunity to produce the BLB-resistant variety. This research aimed to identify BLB disease-resistant gene on local rice germplasm from South Sulawesi based on the association analysis between phenotype resistance and genotype marker of resistance gene to Xoo. The analysis of phenotype resistance testing was done by using three races of BLB, races III, IV, and VIII with IRBB differential varieties. Meanwhile, the genotype analysis was done by using the molecular marker linked to BLB-resistant trait. For linkage analysis between the both of data was done the association analysis between the both of data with Cladogram analysis on Tassel Program. The resistance testing result showed almost all of the local rice in South Sulawesi was resistant to race III, 6 accessions were resistant to race IV and 6 accessions were resistant to race VIII. The association analysis result was known the markers that significant to mark the resistance trait from every race. Marker Xa26-SN2 was significant to mark the resistance trait to race III, the marker Xa1-SN15, Xa4-SN44, Xa13-SN51, and Xa21-SN6 were significant to race IV, marker Xa7-SN57 and RM20589 were significant to race VIII. These markers pottentially as a MAS (Marker Assisted Selection) markers that could be used to aasist the selection of local rice germplasm from South Sulawesi.</jats:p
Faktor Virulensi AvrBs3/PthA pada Ras III, Ras IV, Ras VIII, dan IXO93-068 Patogen Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae)
Get PDFAvrBs3/PthA Virulence Factor of Bacterial Leaf BlightRace III, Race IV, Race VIII, and IXO93-068. Dwinita W.Utami, Triny S. Kadir, and Siti Yuriyah. Bacterial leafblight (BLB) is an important disease of rice and presentthroughout many of the rice-growing regions in the world,also in Indonesia. Xanthomonas oryzae pv. oryzae (Xoo) isthe causal agent and a member of the Protebacteria and likemany other this phyllum have a type III secretion system forprotein virulence effector (PVE) released on their pathogenicitysystem. Commonly, PVE in Xanthomonas sp., iscoded by AvrBs3/PthA family gene. This research wascoducted to identify the virulence factor of AvrBs3/PthA ondominant Indonesian BLB isolates (Race III, Race IV, RasVIII, and IXO93-068). This objective was obtained bysequence analysis through designed markers for membersof the virulence factor AvrBs3/PthA gene family (PthXo4,avrXa7#38, PthXoS and avrXa7sacB50). Results gave informationthat RaceIII is a dependent elicitor race due to noPVE transcript formed and intraceluler protein target withRLL type on NLS (nuclear localization signal). RaceIV andRaceVIII are the virulent race which PVE active formed withintraceluler protein target and have the RLL and RLLP typefor the NLS signal. While isolate IXO93-068 is a virulenisolate that active formed a PVE but the extraceluler proteintarget is due to no type of NLS. Based on cluster analysis,Race VIII has a genetic distance closely to PthXoS andavrXa7sacB50
Evaluasi Dan Identifikasi Marka Penanda Gen Ketahanan Penyakit Hawar Daun Bakteri Pada Padi Lokal Sulawesi Selatan
Get PDFOne of the limiting factors on rice production, especially in South Sulawesi, is the bacterial leaf blight (BLB) disease infection caused by Xanthomonas oryzae pv. oryzae (Xoo). The use of resistant variety is considered as the most effective method to control Xoo. Local cultivar could serve as resistance source for Xoo resistance. The objectives of this research were to evaluate and identify markers for Xoo resistance in rice local cultivars from South Sulawesi based on comparison with the resistance response of the differential varieties and association analysis between resistance phenotype and genotype of molecular markers. Three Xoo races (race III, IV, and VIII) were tested on IRBB differential lines and local cultivars. The genotype analysis was done by using the molecular marker linked to Xoo resistance. Meanwhile, the association analysis was done by combination analysis (U-joint) using generalized linear model (GLM). The resistance test result showed that single gene isogenic lines (IRBB 5, IRBB 7, and IRBB 21), multiple genes isogenic lines (IRBB 50, IRBB 52, IRBB 53, IRBB 54, IRBB 56, IRBB 58, IRBB 64, and IRBB 66), and Ase Andele accession were resistant to the three Xoo races. Association test obtained one significant marker that associated with the resistance to race III (marker Xa26-STS2), four markers significantly associated with the resistance to race IV (Xa1-STS15, Xa4-STS44, xa13-STS51, and Xa21-STS6), and two markers significantly associated with the resistance to race VIII (Xa7-STS57 and RM 20589)
Resistance of Doubled Haploid Rice Lines with Green Super Rice Characters to Bacterial Leaf Blight
Get PDFBacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is a significant disease attacking rice crops worldwide. This disease attacks at various stages of plant growth and causes significant yield loss. Breeding new varieties resistant to BLB is important to support sustainable agriculture in the future. This study aimed to identify new superior green super rice (GSR) lines resistant to BLB disease. The experiment evaluated the resistance of lowland rice lines obtained from anther culture using a factorial randomized complete block design. The 1st factor was genotype, consisting of 20 lines, 2 checks of commercial varieties (Inpari 42 Agritan GSR and Inpari 18), a resistant check (Conde), and a susceptible check (Taichung Native 1). The 2nd factor was BLB pathotypes (i.e., III, IV, and VIII). Quantitative data on disease severity and severity index were analyzed using analysis of variance and t-Dunnett test at 5% level. The results showed that the interaction between genotype and pathotype affected the disease severity and severity index in both growth phases. The tested lines exhibited varying resistance, from susceptible to resistant, to BLB. Four lines (SN 11, 13, 57, and 58) showed moderate to resistant criteria for BLB disease of 3 pathotypes in both growth phases. The selected lines can be used as a source of parents for breeders and candidates for new superior varieties with BLB resistance properties to support the reduction of synthetic chemical bactericide inputs and control BLB disease. However, further field evaluations are necessary to assess their performance
Evaluasi Mutu Fisikokimia Beras Hasil Persilangan antara Empat Padi Varietas Unggul dan Padi Liar (Oryza glaberrima dan O. rufipogon)
Get PDFThe physicochemical quality of rice is evaluated to ensure that it is fit for consumption and meets established quality standards. This study aims to analyze the physicochemical properties of rice strains resulting from crosses between four high-yielding varieties of rice and two wild rices so that the appropriate genotype is obtained for the development of quality rice. The research was conducted from November 2019 to March 2020 at the Quality Laboratory, Muara Experimental Garden, Rice Research Center, Bogor. The rice genotypes used were 22 strains resulting from crosses between high-yielding varieties of rice (Ciherang, Inpari 13, Inpari 10, and Situ Bagendit), and wild rice (Oryza glaberrima and O. rufipogon), 4 comparison varieties of crossbred elders, and two comparison varieties of organoleptic tests (Memberamo and IR42). Physical and chemical qualities were observed in terms of grain moisture content, grain density, broken skin rice yield, milled rice yield, head rice yield, rice length, rice shape, and rice liming, amylose content, gelatinization temperature, and organoleptic. The results showed that the strains 44-F-C21, 58-F-C21, and 59-F-C21 provided fairly good rice quality with head rice yields above 80% and higher than their respective elders (Situ Bagendit and Ciherang), and water content below 14%. These strains had characteristic characteristics, namely medium rice form, amylose content of 26.38%, and alkali score of 2-3. Most of these genotypes also exhibited high gelatinization temperatures, medium rice texture, and savory rice flavors. The genotype selected from the results of crosses between high-yielding varieties of rice and wild rice has the potential to be developed because there are genetic improvements from its elders on several physicochemical characteristics of rice.
Keywords: amylose content; gelatinization temperature; physicochemical properties; rice lines; wild ric
