2,150 research outputs found
Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages
No full textGenetic depletion of macrophages in Polyoma Middle T oncoprotein (PyMT)-induced mammary tumors in mice delayed the angiogenic switch and the progression to malignancy. To determine whether vascular endothelial growth factor A (VEGF-A) produced by tumor-associated macrophages regulated the onset of the angiogenic switch, a genetic approach was used to restore expression of VEGF-A into tumors at the benign stages. This stimulated formation of a high-density vessel network and in macrophage-depleted mice, was followed by accelerated tumor progression. The expression of VEGF-A led to a massive infiltration into the tumor of leukocytes that were mostly macrophages. This study suggests that macrophage-produced VEGF regulates malignant progression through stimulating tumor angiogenesis, leukocytic infiltration and tumor cell invasion
Fidelidad del cliente del Hotel JW Marriott el Convento Cusco -2020
No full textEl presente trabajo de investigación se titula “La fidelidad del cliente del hotel JW Marriott El
Convento Cusco-2020”. Teniendo en cuenta que la fidelidad del cliente es un tema que en la
hotelería es uno de los principales intereses y lo vienen desarrollando la mayoría de las cadenas
hoteleras más renombradas, como es Marriott Hotels y específicamente el hotel JW Marriott El
Convento Cusco. En el cual encontramos un problema del cual tratamos en la presente
investigación, la cual se basa en que: si el cliente no pasa satisfactoriamente las fases de
fidelización, no llega a fidelizarse completamente, ello se debe a que existen ciertos aspectos que
impiden una fidelización, es decir que pueden resquebrajar la fidelidad del cliente, por ejemplo los
inconvenientes que puede tener el cliente, ya sea previo al servicio (desde la reserva del servicio
por medio de Online Travel Agency o por otro medio), la entrega o disfrute del servicio (la estadía)
y el post servicio (después del check out del hotel). Por ello, la presente investigación tiene como
objetivo principal describir la fidelidad del cliente del hotel JW Marriott El Convento Cusco-2020.
Como también, los objetivos específicos que describen la fidelidad del cliente en cada una de las
fases de fidelización. La metodología manejada para la elaboración de la presente tesis fue de tipo
cuantitativo, el nivel es descriptivo no experimental.
Se obtuvo datos del autor (Oliver R. , 1999) sobre las fases de la fidelidad del cliente, que son
fidelidad cognitiva, fidelidad afectiva, fidelidad conativa y fidelidad de acción; las cuales se
utilizaron como dimensiones de la variable, describiendo cada una de ellas. Para describir la
fidelidad del cliente del hotel JW Marriott El Convento Cusco-2020 se realizó encuestas vía web
dada la coyuntura actual, con una muestra no probabilística de 102 personas que fueron los clientes
que voluntariamente respondieron el cuestionario.
Según los resultados obtenidos de las encuestas realizadas, se determinó que la fidelidad del cliente
del hotel JW Marriott El Convento Cusco-2020 es muy buena, al igual que sus cuatro fases de
fidelidad del cliente. Debido a que el hotel cumple con todas las expectativas que tiene el cliente y
al final genera una recompra por parte del cliente hacia el hotel JW Marriott El Convento Cusco,
dado por el cumplimiento de las cuatro fases de fidelización del cliente por lo siguiente: El cliente
es inicialmente fiel en un sentido cognitivo basado en la información y creencia que tiene el cliente,
después de las experiencias satisfactorias se genera un sentimiento de compromiso hacia el hotel
siendo fiel afectivamente, para así por medio de intenciones de comportamientos positivos por parte de asociados del hotel siendo fiel conativa, y finalmente el hotel al cumplir con todas las
expectativas del cliente y superar todos los obstáculos posibles se produce un control de la acción
que viene a ser la fidelidad de acción donde se produce la recompra del servicio del hotel JW
Marriott El Convento Cusco y retener al cliente.
Se pudo concluir que, el hotel JW Marriott El Convento Cusco es muy bueno fidelizando a sus
clientes y puede ser mejor incluso, si se siguen las propuestas planteadas. En la investigación, se
ha logrado describir cómo retener a los clientes y generar un compromiso profundo de recompra
del servicio JW Marriott El Convento Cusco por medio de las fases de fidelización.This research is titled “The costumer loyalty of the JW Marriott El Convento Cusco hotel-2020”.
We know that the loyalty in costumers is one of the most important issues for the hospitality
industry and it is been developed by most of the renowned hotel chains in the World, such as
Marriott Hotels and specifically the JW Marriott El Convento Cusco hotel. We found a problem
wich is based on the fact that: if the costumer doesn´t satisfactorily obtain the four loyalty phases,
the client will not become completely loyal to the hotel. This because there are some aspects that
don´t allow the loyalty, for example the pre service (from the reservation of the service through the
Online Travel agency or other method of reservation), the service (the stay) and the post service
(after the check out of the hotel). The main objective of this research is to describe the customer
loyalty of the JW Marriott El Convento Cusco hotel-2020. As well as, the especific objectives, that
describes the customer loyalty in each of the loyalty phases. The methodology used to prepare this
research was quantitative, and the level is descriptive, not experimental.
The data was obtained from the author (Oliver R. , 1999) that explained about the phases of
customer loyalty, which are Cognitive Loyalty, Afective loyalty, Conative loyalty and Action
loyalty; which were used as dimensions, describing each one of them. In order to describe the
costumer loyalty of the JW Marriott El Convento Cusco hotel-2020, we make the surveys via web
because of the current situation, with a non probability sample of 102 people who were the
costumers who voluntarily answered the questionnaire.
The costumer loyalty of the JW Marriott El Convento Cusco hotel -2020 was determined to be very
good, and also the phases of the customer loyalty as well. All that because the hotel meets all the
expectations that the customer has and in the end generates a repurchase by the customer from the
JW Marriott El Convento Cusco hotel, all that with the four phases of the loyalty of the client, and
we can detail that: The cliente initially gets the cognitive loyalty based in the information and
believes that the client has; after the satisfactory experiences, a feeling of commitment to the hotel
is generated, that is the afective loyalty; and for this reason through the intentions of positive
behaviors of the associates of the hotel, that is the conative loyalty; and finally when the hotel
meets all the expectations of the clients and overcoming all the possible obstacles, there is a control
of the action that comes to be the action loyalty, and the repurchase of the service of the JW Marriott
El Convento Cusco hotel occurs and retain more clients. In conclusion, the hotel JW Marriott El Convento Cusco is very good building customers loyalty
and can be better if the proposals proposed are followed. In the research, it has been possible to
describe how to retain customers and generate a deep commitment to repurchase the service of the
JW Marriott El Convento Cusco hotel through the loyalty phases
The key role of nitric oxide in hypoxia: hypoxic vasodilation and energy supply-demand matching
No full textSignificance: a mismatch between energy supply and demand induces tissue hypoxia with the potential to cause cell death and organ failure. Whenever arterial oxygen concentration is reduced, increases in blood flow - 'hypoxic vasodilation' - occur in an attempt to restore oxygen supply. Nitric oxide is a major signalling and effector molecule mediating the body's response to hypoxia, given its unique characteristics of vasodilation (improving blood flow and oxygen supply) and modulation of energetic metabolism (reducing oxygen consumption and promoting utilization of alternative pathways). Recent advances: this review covers the role of oxygen in metabolism and responses to hypoxia, the hemodynamic and metabolic effects of nitric oxide, and mechanisms underlying the involvement of nitric oxide in hypoxic vasodilation. Recent insights into nitric oxide metabolism will be discussed, including the role for dietary intake of nitrate, endogenous nitrite reductases, and release of nitric oxide from storage pools. The processes through which nitric oxide levels are elevated during hypoxia are presented, namely (i) increased synthesis from nitric oxide synthases, increased reduction of nitrite to nitric oxide by heme- or pterin-based enzymes and increased release from nitric oxide stores, and (ii) reduced deactivation by mitochondrial cytochrome c oxidase. Critical issues: several reviews covered modulation of energetic metabolism by nitric oxide, while here we highlight the crucial role NO plays in achieving cardiocirculatory homeostasis during acute hypoxia through both vasodilation and metabolic suppression Future directions: we identify a key position for nitric oxide in the body's adaptation to an acute energy supply-demand mismatc
Comment on "Wandering minds: The default network and stimulus-independent thought"
No full textMason et al. (Reports, 19 January 2007, p. 393) attributed activity in certain regions of the "resting" brain to the occurrence of mind-wandering. However, previous research has demonstrated the difficulty of distinguishing this type of stimulus-independent thought from stimulus-oriented thought (e.g., watchfulness). Consideration of both possibilities is required to resolve this ambiguity
The impact of neutralizer-free ignition of a radio frequency ion thruster on the lifetime of the ion optics system
No full textIn the initial stage of a radio frequency ion thruster (RIT) ignition, an influx of electrons is required from an external source into the discharge chamber and ionization of the neutral gas propellant. A neutralizer-free method for Townsend breakdown discharge ignition based on Paschen's law was developed in this study. The feasibility of the ignition method was confirmed by performing thousands of ignition experiments. Metallic Molybdenum (Mo), pyrolytic graphite (PG) and Zr41.2Ti13.8Cu12.5Ni10Be22.2 alloy acceleration grids were prepared, and ignition-induced damage on the grids was investigated. A field-emission scanning electron microscope was used to inspect surface damage on the grids after multiple ignitions and to analyze the influence of the ignition method on the lifetime of the ion optical system. Grid materials for space missions that require multiple RIT ignitions (10(3)) should be high-strength blocks that are resistant to sputtering corrosion and high temperature.</p
Role of BMP3 in progression of gastric carcinoma in Chinese people
No full textAuthor contributions: Chen XR designed the study and wrote the manuscript; Wang JW performed the majority of experiments; Li X and Wang JW provided the vital reagents and analytical tools and were also involved in editing the manuscript; Zhang H and Ye ZY coordinated and provided the collection of all human materials in addition to financial support for this work
Detection of monosomy 7 in interphase cells of patients with myeloid disorders
No full textSix patients, five with acute myeloid leukemia (AML) and one with a myelodysplastic syndrome (MDS), were found to have monosomy 7 by conventional cytogenetics at diagnosis. Repetitive DNA sequences from the heterochromatic region of human chromosomes 1 and 7 were used as probes for in situ hybridization experiments on interphase cells of these patients. A double hybridization protocol was used to reveal the particular chromosomes as distinct spots or clusters of signals within interphase nuclei. The chromosome 1 sequence served as an internal control. Simultaneous detection of the sequences showed the presence of two normal number 1 chromosomes and a missing 7 chromosome from individual cells. While cytogenetic preparations showed only -7 metaphases in 3 AML and 1 MDS patients, in situ hybridization of interphase cells showed many normal cells as well as the presence of -7 in fully mature granulocytes. One AML patient studied in remission showed only normal metaphases yet had 9% interphase cells with a missing 7 and relapsed within 3 months. We conclude that examination of interphase cells by in situ hybridization provides clinically useful data since every cell including mature granulocytes can be examined, the lineage of a cell can be determined, and efficacy of differentiation therapy can be evaluated
Amenouahsou et les deux Sobek de Gebelein. Prosopographie et géographie religieuse d’Jnr.ty à Jw-Mjtrwآمن-واح-سو مع المعبودين على هيئة سوبك بمنطقة الجبليْن. البروزوبوغرافيا
No full textInternational audienceتهدف هذه الدراسة إلى زيادة فهم اللقب الكهنوتي الخاص بكبير الكهنة «آمون-واح-سو» المُعاصر لفترة حُكم مرنپتاح. لقد كان الكاهن الأوّل للعديد من المعبودات التي ما زالت هويتها مجهولة حتّى الآن بسبب أسمائها، حيث استخدمت في بعض الأوقات العلامات التصويريّة فقط في كتابتها.تمّ تحديد هذه المعبودات والتعرُّف عليها بكونها: أنوبيس، وخونسو، والمعبودين على هيئة سوبك. توضّح هذه الدراسة، التي استندت على عدّة أنواع من المصادر، أنَّ مكان عبادتهم جميعًا هي منطقة الجبلين، على وجه التحديد في مدينة Swmnw سومنو (حيث مكان عبادة سوبك) و Jw-Mjtrw إو-ميترو (حيث مكان عبادة سوبك وأنوبيس وخونسو). علاوة على ذلك، يقترح المؤلف أن معبد سومنو قد تمَّ نقله في بداية عصر الدولة القديمة بسبب التغيُّرات الحادثة في مجرى النيل. قد تفسر هذه الظاهرة الجغرافيا الدينيَّة المُعقَّدة، بالإضافة إلى الصعوبات الناتجة التي تواجه الأثريّين الدارسين لهذه المعتقدات حتّى اليوم.The aim of this study is to better understand the pontifical titles of the high priest Jmn-wȝḥ-sw, contemporary of Merenptah. He was the first prophet of several deities whose identification has remained uncertain until now, since their names are sometimes written only with ideograms. First, these deities are identified as Anubis, Khonsu and “two Sobeks”. Secondly, the study, which is based on several types of sources, shows that they were all worshipped in the Gebelein region, more precisely in the cities of Swmnw (Sobek) and Jw-Mjtrw (Sobek, Anubis and Khonsu). Furthermore, the author proposes that the temple of Swmnw was moved at the beginning of the New Kingdom due to changes in the course of the Nile. This phenomenon would be at the origin of a complex religious geography, as well as the cause of difficulties for Egyptologists who study these cults until today.L’objectif de cette étude est de comprendre au mieux le titre pontifical du grand prêtre Jmn-wȝḥ-sw, contemporain de Mérenptah. Il officiait comme premier prophète de plusieurs divinités dont l’identification est restée jusqu’à présent incertaine en raison de leurs noms, parfois écrits seulement avec des idéogrammes. Ces divinités sont identifiées comme étant Anubis, Khonsou et « deux Sobek ». L’étude proposée, qui se fonde sur plusieurs types de sources, montre qu’elles recevaient toutes un culte dans la région de Gebelein, plus précisément dans les cités de Swmnw (Sobek) et Jw-Mjtrw (Sobek, Anubis et Khonsou). L’auteur envisage la possibilité que le temple de Swmnw ait été déplacé au début du Nouvel Empire à cause de changements qui auraient affecté le cours du Nil. Ce phénomène expliquerait une géographie religieuse complexe, de même que les difficultés des égyptologues à étudier ces cultes jusqu’à aujourd’hui
An efficacy and mechanism evaluation study of Levosimendan for the Prevention of Acute oRgan Dysfunction in Sepsis (LeoPARDS): protocol for a randomized controlled trial.
No full textBACKGROUND: Organ dysfunction consequent to infection ('severe sepsis') is the leading cause of admission to an intensive care unit (ICU). In both animal models and early clinical studies the calcium channel sensitizer levosimendan has been demonstrated to have potentially beneficial effects on organ function. The aims of the Levosimendan for the Prevention of Acute oRgan Dysfunction in Sepsis (LeoPARDS) trial are to identify whether a 24-hour infusion of levosimendan will improve organ dysfunction in adults who have septic shock and to establish the safety profile of levosimendan in this group of patients. METHODS/DESIGN: This is a multicenter, randomized, double-blind, parallel group, placebo-controlled trial. Adults fulfilling the criteria for systemic inflammatory response syndrome due to infection, and requiring vasopressor therapy, will be eligible for inclusion in the trial. Within 24 hours of meeting these inclusion criteria, patients will be randomized in a 1:1 ratio stratified by the ICU to receive either levosimendan (0.05 to 0.2 μg.kg⁻¹.min⁻¹ or placebo for 24 hours in addition to standard care. The primary outcome measure is the mean Sequential Organ Failure Assessment (SOFA) score while in the ICU. Secondary outcomes include: central venous oxygen saturations and cardiac output; incidence and severity of renal failure using the Acute Kidney Injury Network criteria; duration of renal replacement therapy; serum bilirubin; time to liberation from mechanical ventilation; 28-day, hospital, 3 and 6 month survival; ICU and hospital length-of-stay; and days free from catecholamine therapy. Blood and urine samples will be collected on the day of inclusion, at 24 hours, and on days 4 and 6 post-inclusion for investigation of the mechanisms by which levosimendan might improve organ function. Eighty patients will have additional blood samples taken to measure levels of levosimendan and its active metabolites OR-1896 and OR-1855. A total of 516 patients will be recruited from approximately 25 ICUs in the United Kingdom. DISCUSSION: This trial will test the efficacy of levosimendan to reduce acute organ dysfunction in adult patients who have septic shock and evaluate its biological mechanisms of action. TRIAL REGISTRATION: Current controlled trials ISRCTN12776039 (19 September 2013)
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