42 research outputs found
Chromatin Landscapes of the Dlx1/2 and Dlx5/6 Bigene Clusters in the Developing Mouse Forebrain
No full textThe Distal-less (Dlx) homeobox genes of mammals are expressed in many tissues of the developing organism including the limbs, craniofacial skeleton and the forebrain. In the forebrain, Dlx1, Dlx2, Dlx5 and Dlx6 play a critical role in driving tangential migration of GABAergic progenitors from the ventral telencephalon to their final destinations, notably the neocortex and the striatum. These Dlx genes are organised into convergently transcribed clusters with short intergenic regions that contain notable cis regulators elements (CREs) that drive Dlx expression in unique subdomains of the developing ventral telencephalon. Previous studies have characterised Dlx regulation including but not limited to the direct activation of these CREs by effector proteins. However, to date very little work has been done to examine how the forebrain Dlx genes may be regulated at the level of the chromatin. To explore this, I used in silico and in vivo methods to examine some key histone modifications of the Dlx1/2 and Dlx5/6 bigene clusters in the developing forebrain; namely H3K27Ac, H3K4me3, H3K4me1 and H3K27me3. I found that within the Dlx expressing ganglionic eminences (GE), at midgestation, the Dlx loci are marked by bivalent chromatin which is enriched in both permissive H3K4me3 and repressive H3K27me3 marks. By performing ChIP-qPCR on the GE tissue of embryonic mice with targeted deletions of enhancer CREs, I found that these CREs play unique roles in shaping the chromatin. Removal of one of these CREs has widespread effects on the chromatin at both loci. Since these changes in chromatin signatures do not accompany significant changes in expression of histone modifying genes, we believe these CREs play yet-to-be determined roles in recruiting the modifying proteins to the loci, thereby establishing bivalent chromatin to fine-tune Dlx expression
Designing and developing a smart commuting application to support healthy lifestyles
No full textObesity is one of the great challenges of modern civilization. Heart disease, diabetes and other conditions related to obesity are already a major problem. People become lazy when encouraged to use their own car instead of public transport or walking to work: this leads to more problems, such as traffic congestion, pollution and parking issues. The aim of this thesis is to design and develop the basic required characteristics, functionalities, options and features of an application which can be used by companies to provide incentive to their employees and provide analytics about the wellbeing of these employees. It is all about incentivizing people and organizations to make choices that have a positive impact on the environment. The mobile application Cleancentive was designed, developed and tested for companies so they can support their employees with their physical health activities.
The study shows that while testing the application, the daily routine and lifestyle of the author was benefitted, bringing more energy for the day and also increasing productivity. It was also found that incentive models may need to be customised on a company-by-company basis to find out which model works best for them, always bearing in mind that people tend to get motivated and excited when they’re getting some kind of financial reward
The Beloved of Emperor SHAHJAHAN of the MOGHAL DYNASTY JAHAN ARA BEGUM, As a Writer: خاندان مغلیہ کے تاجدارشاہجہاں کی نورِچشم جہاں آراء بیگم بطور ادیبہ
No full textJahan Ara Begum was the daughter of Emperor ShahJahan of the Mughal dynasty. She was a writer, with a greater inclination towards spirituality. She wrote “Monis-e-Arwah” and “Sahbiya” treatises about revered saints. Monis-e-Arwah” is a compilation of the sayings and incidents related to the Chishti Saints, where her second work is one in which Jahan Ara Begum reflected on her own spiritual life. A manuscript of this book is available in a library of Ahmadabad, while another is housed in the Asifia Library.
These treatises are adorned with all the beauties of language and expressions. In terms of interest, they hold significant importance the efforts of a Princess from the Royal family as an author are commendable. In these literary treatises, the expressions of sincerity, realism, self-discovery, emotional states, sentiments and observations are presented with great subtlety. In her writings, all the qualities of thoughts and artistry, vivid imagery devotion and captivating styles of expression are present
A note on nontrivial intersection for selfmaps of complex Grassmann manifolds
No full textLet G(k,n) be the complex Grassmann manifold of k-planes in Ck+n. In this note, we show that for 1 < k < n and for any selfmap f: G(k,n) → G(k,n), there exists a k-plane Vk ∈ G(k,n) such that f(Vk) ∩ Vk ≠ {0}.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) Institute of Geosciences and Exact Sciences (IGCE), Av. 24A, 1515 Bela VistaDepartment of Mathematics Bates CollegeSão Paulo State University (UNESP) Institute of Geosciences and Exact Sciences (IGCE), Av. 24A, 1515 Bela VistaCAPES: 88881.068085/2014-01CAPES: 88887.113937/2015-0
Accordéon succès N °I
No full textTitre uniforme : [La fête des fleurs = El humahuaqueno]Titre uniforme : [Toi qui disais]Comprend : Faubourg Saint-Martin - valse / R. MONIS ; Jo PRIVAT et son orchestre - Les amoureux du Havre - valse / Léo FERRE ; le Duo NICOLI - Toi qui disais, qui disais, qui disais - fox / Dana SUESSE ; Jo PRIVAT et son orchestre - Anne-Lise - marche / Hans-Arno SIMON ; le Duo NICOLI - Le tango des jours heureux : tango / M. HARDEN ; Jo PRIVAT et son orchestre - Mes mains - boléro / Gilbert BECAUD ; le Duo NICOLI - La fête des fleurs ("El humahuaqueno") du film "Tourments" baïao / Edmundo P. ZALDIVAR - La musique à papa ("Que le quiten el Tapon") samba guaracha / M. JIMENEZ ; ALEXANDER et son orchestre - Tout ça parc' qu'au bois d'Chaville - valse / C. ROLLAND ; Jo PRIVAT et son orchestre - Printemps d'Alsace - valse / L. LEDRICH ; Jo PRIVAT et son Orchestre - Fleur de papillon : fox / Jean CONSTANTIN ; Jo PRIVAT et son orchestre - La goualante du pauvre Jean / Marguerite MONNOT ; Jo PRIVAT et son orchestre - Un petit nuage : boléro / Francis LOPEZ ; André ASTIER et son orchestre - Thumbeline : du film "Hans Christian Andersen" marche / F. LOESSER ; le Duo NICOLI - Emmène-moi avec toi : tango / M. DENOUX ; André ASTIER et son orchestre - Johnny, tu n'es pas un ange : fox-trot / Les PAUL ; ALEXANDER et son orchestreBnF-Partenariats, Collection sonore - BelieveContient une table des matière
Genotyping of Cryptosporidium spp. isolated from human stool samples in Switzerland
No full textIn a study to estimate the frequency of Cryptosporidium infections in Switzerland, stool samples from patients found to be positive for Cryptosporidium spp. by modified Ziehl-Neelson staining and fluorescence microscopy were used for genotyping experiments. With 9 of 12 samples, DNA extraction and subsequent genotyping was successful. All Cryptosporidium-isolates belonged to the bovine genotype. In one stool sample, two strains of Cryptosporidium were demonstrated, suggesting a mixed infection. In comparison with reference strains from calves, one of the isolates showed a full sequence identity and the other a similarity of 97.5%. The fact that only bovine genotypes were detected suggests, that cryptosporidiosis must primarily be considered as a zoonotic disease in Switzerland. This is in contrast to other countries, where the human genotype of C. parvum was shown to dominate the epidemiological situation. The results of our study are supported by the previous finding, that two of the analysed strains originated from patients who used to consume raw milk or raw cream, a known risk factor for cryptosporidiosis
