14 research outputs found
Efikasi Burkholderia cepacia GL3 dalam Memacu Pertumbuhan Tanaman Kedelai (Glycine max)
Full text linkBurkholderia cepacia sp. GL3 population naturally occurring in the rhizosphere of Glyricidia sepium was evaluated by metabolic and molecular profiling and for some traits associated with plant growth promoting (PGP) and phosphate solubilizing activity. This research were performed to investigate the effect of inoculation of phosphate solubilizing bacteria (B. cepacia sp. GL3), VAMycorrhizal, Rhizobium bacteria associated with soybean, consortium of potential microbes, combination of PSB and VA-Mycorrhizal, combination of VA-Mycorrhizal and Rhizobium, combination of PSB and Rhizobium, and combination of PSB, VA-Mycorrhizal and Rhizobium with the same concentration toward the growth of soybean plant (Glycine max). The research using polybags with 10 kgs capacity of soil, at the research field of Research center of Biotechnology-LIPI, Cibinong. Parameters used are plant height, number of pods, weight of pods, and weight of seed. This research is experimental research using RAL factorial (10x4). Analysis data using SAS statistic analysis, and then using Duncan test 5%. Results demonstrated that, all treatments displayed significant effect toward plant height, weight of pods, and weight of seed. But it had no significant effect on number of pods. Inoculation of PSB showed significant effect toward plant height, number of pods, weight of pods, and weight of seed as compared to inoculation of VAMycorrhizal and negative control (with chemical fertilizer and uninoculated plant). Percentage increase in soybean seed weight of PSB inoculation plant compare to inoculated plant 47,9%, three months after inoculation. Moreover, the combination of five potential microbes were able to positively affect that showed significantly values of weight of seed, as compared with single microbe inoculation and uninoculated plants. The weight of soybean seed could show productivity of soybean.Keywords: Burkholderia cepacia sp. GL3, growth promotion, phosphate solubilizing bacteria on soybean plan
ISOLASI MIKROBA ENDOFITIK DARI TANAMAN OBAT SAMBUNG NYAWA Gynura procumbens) DAN ANALISIS POTENSINYA SEBAGAI ANTIMIKROBA
No full textSambung nyawa (Gynura procumbens) has many beneficial effects to human health, such as decreasing blood pressure, maintaining blood sugar level (hypoglycaemic), decreasing cholesterol, a remedy for kidney trouble, antibacterial and lessen the inflamation (antiinflamation). This research was undertaken to discover the potency of endophytic microbes from sambung nyawa as antimicrobial agents. The purpose of this research was to screen the endophytic bacteria and the endophytic fungi having antimicrobial activity, which were isolated from stems, leaves, roots and fruits of medical plants, sambung nyawa. The antimicrobial activity was determined by measuring the growth inhibition of pathogenic microbes i.e Candida albicans, Escherichia coli, Pseudomonas sp. and Bacillus subtilis. A total of 38 isolates of bacteria and 15 isolates of fungi were obtained from sambung nyawa. Analysis demonstrated that, 45 percent isolates of bacteria and 20 percent isolates of fungi exhibited inhibitory activity. Antimicrobial activity was found in 21 percent of the isolates that inhibited the growth of C. albicans, E. coli, Pseudomonas sp., and B. subtilis, whereas 24 percent of isolates had activity only against B. subtilis. Isolate of endophytic bacteria USN 1.1 and USN 2.3 showed the most significant of inhibition zone. The Inhibition zone of the isolate USN 1.1 to C. albicans, E. coli, Pseudomonas sp, and B. subtilis were 2.318 cm2, 0.969 cm2, 0.796 cm2, and 0.381 cm2, respectively. The Inhibition zone of the isolate USN 2.3 to C. albicans, E. coli, Pseudomonas sp., and B. subtilis were 3.01 cm2, 0.519 cm2, 0.588 cm2 and 0.83 cm2, respectively. These results indicated that endophytic bacteria and endophytic fungi could be a promising source for antimicrobial agents.</jats:p
Identifikasi dan Kekerabatan Rhizobia Pohon Mangium dan Sengon Berdasarkan nodD1 dan nifH
Full text linkRhizobia from legumes Acacia mangium (Mangium) and Paraserianthes falcataria (Sengon) have often been isolated and studied for their applications to plants, but studies on the nod and nif genes are still lacking. Even though this plant were often used as a source of paper raw materials and reforestation plants. The aim of this study was to define the genetic relationship of a group of potential strains isolated from tropical legume trees in terms of 16S rRNA, nodD1, and nifH genes. This research method includes the selection of isolates based on the main character of Rhizobiales, to isolate the 16S rRNA, nodD1, and nifH genes from the selected isolates, and to construct a phylogeny tree based on the isolated genes. Two rhizobia were selected based on a selection test, namely DCM 212 from A. mangium and DF13 from P. falcataria. DCM 212 isolate was identified as having the closest similarity to Rhizobium multihospitium CC-13H. The isolate of DF13 had high similarity with Bradyrhizobium elkanii based on 16S rRNA, nodD1, and nifH. The degenerative primer pairs used in this study could not detect nodD1 gene from DCM 212 isolate.
Keywords: Bradyrhizobium, phylogeny, rhizobiu
Isolasi mikroba endofitik dari tanaman obat sambung nyawa (Gynura procumbens) dan analisis potensinya sebagai antimikroba
No full textSambung nyawa (Gynura procumbens) has many beneficial effects to human health, such as decreasing blood pressure, maintaining
blood sugar level (hypoglycaemic), decreasing cholesterol, a remedy for kidney trouble, antibacterial and lessen the inflamation (antiinflamation).
This research was undertaken to discover the potency of endophytic microbes from sambung nyawa as antimicrobial
agents. The purpose of this research was to screen the endophytic bacteria and the endophytic fungi having antimicrobial activity,
which were isolated from stems, leaves, roots and fruits of medical plants, sambung nyawa. The antimicrobial activity was determined
by measuring the growth inhibition of pathogenic microbes i.e Candida albicans, Escherichia coli, Pseudomonas sp. and Bacillus
subtilis. A total of 38 isolates of bacteria and 15 isolates of fungi were obtained from sambung nyawa. Analysis demonstrated that,
45% isolates of bacteria and 20% isolates of fungi exhibited inhibitory activity. Antimicrobial activity was found in 21% of the
isolates that inhibited the growth of C. albicans, E. coli, Pseudomonas sp., and B. subtilis, whereas 24% of isolates had activity only
against B. subtilis. Isolate of endophytic bacteria USN 1.1 and USN 2.3 showed the most significant of inhibition zone. The Inhibition
zone of the isolate USN 1.1 to C. albicans, E. coli, Pseudomonas sp, and B. subtilis were 2.318 cm2, 0.969 cm2, 0.796 cm2, and 0.381
cm2, respectively. The Inhibition zone of the isolate USN 2.3 to C. albicans, E. coli, Pseudomonas sp., and B. subtilis were 3.01 cm2,
0.519 cm2, 0.588 cm2 and 0.83 cm2, respectively. These results indicated that endophytic bacteria and endophytic fungi could be a
promising source for antimicrobial agents
Identification of 1-Aminocyclopropane-1-Carboxilid Acid (ACC)-Deaminase Producing Endophytic Bacteria from Local Agricultural Plantation Based on 16S Ribosomal RNA Gene as Genetic Marker
Full text linkThe objective of this work was to isolate and identify of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase producing endophytic bacteria from root of local agricultural plantation by using 16S rRNA gene as genetic marker. Twelve root samples were collected from local agricultural plantation in Java area. After surface sterilization, each root sample was inoculated in nutrient agar media and the grown colonies were further purified and tested for the ability to grow in N-free minimal medium containing AIB as a sole of nitrogen source. The selected colonies were further tested for their ACC deaminase activity by measuring the rate of conversion of ACC into α-ketobutyrate. From this work, 12 bacterial strains that exhibited the ACC deaminase activity by 123.75 to 1461.44 nmol α-ketobutyrate/mg/hour were successfully isolated. Based on the 16SrRNA gene sequences, those bacterial isolates were identified as Sphingobacterium multivorum BK1, Bacillus mycoides CB2, Pantoea dispersa CK4, Pantoea agglomerans KD6.2, Enterobacter ludwigii KW3, Bacillus aryabhattai TW7, Pseudomonas monteilii KS12, Pseudomonas plecoglossicida KS16.2, Pseudomonas putida PIR3C, Stenotrophomonas maltophilia PIR5, Lysinibacillus pakistanensis PIC5, and Raoultella terrigena PCM8. Pseudomonas putida PIR3C and Pseudomonas monteilii KS12 showed promising ACC deaminase activity and therefore it could be as a good candidate for further application in plant growth promoting in stress conditions
Endophytic bacteria from paddy with double 1-aminocyclopropane-1-carboxylic acid deaminase and nitrogenase activity
No full textPlant growth promoting bacteria with dual activity, 1-aminocyclopropane-1-carboxylic-acid deaminase (ACCD) and nitrogenase, is more effective in supporting plant growth under stress condition. Previously, we were obtained several endophytic bacterial strains that exhibited dual activity, one of which was Raoultella terrigena PCM8. This study aimed to characterize the ACCD and nitrogenase genes of PCM8 strain. The acdS gene was obtained from the results of Whole Genomic Sequencing analyis, while the nifH gene was obtained by PCR. The characterization of both of the genes was carried out by means of in-silico analysis. WGS annotation analysis, showed that the acdS gene of PCM8 was located at the locus 19090 of genomic DNA and contains 978 nucleotides. In silico analysis of both acdS and nifH gene products showed that the ACCD enzyme of PCM8 had 325 amino acids, with molecular weight of 34.95 kDa, while nitrogenase as represented by nifH subunit product consist of 96 amino acids with molecular weight of 93.98 kDa, respectively. The ACCD had pI value of 5.06, and catalytic residues of Lys51, Ser78, Tyr287, and Thr288, while nifH gene product had the pI value of 11.77. The results suggested that R. terrigena PCM8 potentially produce double activity of ACCD and nitrogenase and therefore it can be a good candidate as plant growth promoting under stress condition.Keywords: acdS gene, 1-aminocyclopropane-1-carboxylic acid deaminase, endophytic bacteria, nifH gene, nitrogenase Raoultella terrigen
Indigenous bacterial bioremediation: Chromiumreducing capabilities of native strains from Halmahera
Full text linkIndonesia, with its abundant natural resources, faces significant environmental degradation from open-pit mining, that lead to deforestation and heavy metal contamination, which pose dangers to humans and ecosystem. While bacterial bioremediation of heavy metals is well-documented, studies on chromium bioremediation using indigenous Indonesian bacteria remains scarce. This study characterizes chromium-reducing bacteria isolated from post-mining soil in Halmahera, Indonesia, and evaluates their potential for both bioremediation and reforestation of post-mining lands. Macroscopic analyses were used to determine the genus of the Halmahera bacterial isolates and their chromium reducing-capability was assessed using Diphenylcarbazide (DPC) method. Notably these isolates achieved a chromium reduction efficiency of up to 39%, slightly lower than 50% reduction by Pseudomonas aeruginosa in another study, as well as exhibiting phosphate solubility index of 0.66, which shows the bacteria potential to promote plant growth. Our findings demonstrate that these indigenous bacteria are promising candidates for sustainable bioremediation efforts, effectively reducing chromium contamination and simultaneously accelerating reforestation in post-mining areas. This dual action contributes to developing scalable, eco-friendly strategies to mitigate environmental damage caused by mining in Indonesia and similar post-mining environment globally, potentially paving the way for industrial applications in environmental restoration
Indigenous bacterial bioremediation: Chromiumreducing capabilities of native strains from Halmahera
No full textIndonesia, with its abundant natural resources, faces significant environmental degradation from open-pit mining, that lead to deforestation and heavy metal contamination, which pose dangers to humans and ecosystem. While bacterial bioremediation of heavy metals is well-documented, studies on chromium bioremediation using indigenous Indonesian bacteria remains scarce. This study characterizes chromium-reducing bacteria isolated from post-mining soil in Halmahera, Indonesia, and evaluates their potential for both bioremediation and reforestation of post-mining lands. Macroscopic analyses were used to determine the genus of the Halmahera bacterial isolates and their chromium reducing-capability was assessed using Diphenylcarbazide (DPC) method. Notably these isolates achieved a chromium reduction efficiency of up to 39%, slightly lower than 50% reduction by Pseudomonas aeruginosa in another study, as well as exhibiting phosphate solubility index of 0.66, which shows the bacteria potential to promote plant growth. Our findings demonstrate that these indigenous bacteria are promising candidates for sustainable bioremediation efforts, effectively reducing chromium contamination and simultaneously accelerating reforestation in post-mining areas. This dual action contributes to developing scalable, eco-friendly strategies to mitigate environmental damage caused by mining in Indonesia and similar post-mining environment globally, potentially paving the way for industrial applications in environmental restoration
