1,721,013 research outputs found
Low Frequency - High Sensitivity Horizontal Inertial Sensor based on Folded Pendulum
No full textThis paper describes a new implementation of monolithic horizontal sensor, developed at the University of Salerno, based on the Folded Pendulum architecture, configurable both as seismometer and as accelerometer. The large low-frequency band (10-6 ÷ 10 Hz), the high sensitivity (10-12 m/sqrt(Hz) in the band 0.1 ÷ 10 Hz) and the high quality factor in air (Q > 1500) are largely better than all the previous Folded Pendulum implementations. Moreover its monolithic implementation of the whole mechanics, coupled with a full tunability of its resonance frequency (70 mHz ÷ 1.2 Hz) obtained with a specially designed calibration procedure and with an integrated laser optical readout, guarantees both compactness, robustness and immunity to environmental noises. This makes this sensor suitable for a large number of scientific applications, also in high vacuum and cryogeny. Applications of this sensor are already started in the field of geophysics, including the study of seismic and newtonian noise for characterization of suitable sites for future underground interferometric detectors of gravitational waves
Comparison of 1H-NMR spectra by normalisation algorithms for studying amyloid toxicity in cells
No full textA range of debilitating human diseases is associated with the formation of stable, highly organised, protein aggregates, the amyloid fibrils. Substantial evidence suggests that Prefibrillar Oligomeric Aggregates (PFAs), preceding mature fibrils formation, are the crucial species in the onset of the neuronal degeneration even if with mechanisms to be further cleared. In this work, we show how 1H-NMR cell spectral analysis methods can prove to be very effective tools to clear the PFAs amyloid cytotoxicity mechanisms. Following the same method shown by Vilasi, we apply a new 1H-NMR analysis algorithm to identify the metabolites significantly varied in cells incubated with toxic oligomers from the amyloidogenic W7FW14F mutant of apomyoglobin. Our main aim is to confirm the results obtained by Vilasi et al.,
normalising a set of different data spectra with the new PRICONA algorithm
here described, thus contributing to strengthen the general framework of
metabolites and proteins involved in cellular amyloid toxicity
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
RESTORING MMACHC EXPRESSION: TRIDs AS A PROMISING APPROACH FOR cblC DEFICIENCY TREATMENT
No full textRare genetic diseases, often defined as ''orphan diseases'' due to their low influence and limited therapeutic options, have always represented a demanding challenge for research and medicine. Recent attention has focused on stop mutations, one of the different classes of mutations responsible for these pathologies. This gene defect, considered among the most harmful, determines the formation of premature termination codons (PTCs), whose presence on the coding mRNA results in the synthesis of a non-functional truncated protein or with alterations that can compromise its functioning biological activity. One of the most promising approaches to counteracting these mutations is based on the use of a new class of molecules, known as Translational Readthrough-Inducing Drugs (TRIDs), which interfere with protein translation, allowing the synthesis of a complete and potentially functional protein. This study aimed to evaluate the readthrough activity of 3 new TRID molecules (NV848, NV914, and NV930) to rescue the expression of the cobalamin chaperone MMACHC protein associated with the metabolism of vitamin B12. We engineered HCT116 cells with two vectors harboring the R132X and Y222X mutations in the MMACHC cDNA and treated them with TRIDs at different time points. After 24, 48, and 72 hours of TRID treatments, Real-Time RT-PCR analysis revealed an increase in the expression levels of mutated MMACHC mRNA, suggesting a possible transcript stabilization. Immunofluorescence analyses further supported these data, confirming the protein's partial rescue. These results suggest that the analyzed TRIDs could represent a promising therapeutic option for the treatment of cobalamin C (cblC) deficiency disease. Furthermore, such evidence paves the way for developing personalized therapeutic strategies based on the readthrough of premature stop codons, offering an innovative perspective for treating numerous rare genetic diseases currently without effective treatments
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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