1,721,305 research outputs found
Effectiveness, Against Tuberculosis, Of Pseudo-ternary Complexes: Peptide-dna-cationic Liposome
No full textWe report the effects of a synthetic peptide designed to act as a nuclear localization signal on the treatment of tuberculosis. The peptide contains 21 amino acid residues with the following specific domains: nuclear localization signal from SV 40T, cationic shuttle sequence, and cysteamide group at the C-terminus. The peptide was complexed with the plasmid DNAhsp65 and incorporated into cationic liposomes, forming a pseudo-ternary complex. The same cationic liposomes, composed of egg chicken l-α-phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium-propane, and 1,2-dioleoyl-3-trimethylammonium-propane (2:1:1. M), were previously evaluated as a gene carrier for tuberculosis immunization protocols with DNAhsp65. The pseudo-ternary complex presented a controlled size (250. nm), spherical-like shape, and various lamellae in liposomes as evaluated by transmission electron microscopy. An assay of fluorescence probe accessibility confirmed insertion of the peptide/DNA into the liposome structure. Peptide addition conferred no cytotoxicity in vitro, and similar therapeutic effects against tuberculosis were seen with four times less DNA compared with naked DNA treatment. Taken together, the results indicate that the pseudo-ternary complex is a promising gene vaccine for tuberculosis treatment. This work contributes to the development of multifunctional nanostructures in the search for strategies for in vivo DNA delivery. © 2011 Elsevier Inc..3731102109Gomes, D.C.D., Pinto, E.F., de Melo, L.D.B., Lima, W.P., Larraga, V., Lopes, U.G., Rossi-Bergmann, B., (2007) Vaccine, 25, p. 2168Bao, L., Gao, L., Bao, Y., (2010) Int. J. Infect. Dis., 14, pp. E446Han, D.J., Weiner, D.B., Sin, J.I., (2010) Biomol. 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Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Distribution Of Environmental Compartments Of Herbicides Used In The Cotton, Coffee And Citrus Cultures [distribuição Nos Compartimentos Ambientais Dos Herbicidas Utiilizados Nas Culturas De Algodão, Café E Citros]
No full textThe aim of this paper was to evaluate the environmental fate of herbicides (acetochlor, 2,4-D, diuron, clomazone, thidiazuron, paraquat, simazine, fluazifo-p-butil, clethodim, oxufluorfen, flumioxazin, carfentrazone-ethyl, ametrina, trifluralin and MSMA) in cotton, coffee and citrus cultivation areas, applying the level I fugacity model. The methodology basically used the chemical and physical characteristics of the pesticides, environmental compartments and the fugacity equations. The preliminary evaluation of risk of contamination due to the use of these pesticides on the cultures studied was carried out swiftly, based on the chemical and physical properties of these products as the level I fugacity model was applied. For most of the herbicides evaluated, the water compartment was the most vulnerable. The prediction evaluation study using fugacity level I was found to be a relevant tool for the environmental fate of the herbicides studied for cotton, coffee and citrus cultures.271123132Bru, R., Carrasco, J.M., Paraíba, L.C., Unsteady state fugacity model by a dynamic control system (1998) Appl. Math Model., 22 (7), pp. 485-494Calamari, D., Vighi, M., Bacci, E., The use of terrestrial plant biomass as a parameter in the fugacity model (1987) Chemosphere, 16 (10-12), pp. 2359-2364Mackay, D., Paterson, S., Calculating fugacity (1981) Environ. Sci. Technol., 15 (9), pp. 1006-1014Mackay, D., (1991) Multimedia Environmental Models: The Fugacity Approach, p. 257. , Boca Raton: Lewis PublishersParaíba, L.C., (1999) Modelización de la Fugacidad Mediante Sistemas Dinámicos Discretos, p. 199. , Tesis (Doctoral) - Universidad Politécnica de Valência, Valência, 1999Paraíba, L.C., Carrasco, J.M., Bru, R., Level IV fugacity model by continuous time control system (1999) Chemosphere, 38 (8), pp. 1763-1775Paraíba, L.C., Simulation of the fate of the insecticide carbifuran in a rice field using a level IV fugacity model (2007) Span J Agric Res., 5 (1), pp. 43-50Plese, L.P.M., Silva, C.L., Foloni, L.L., Previsão da distribuição ambiental dos herbicidas utilizados na cultura da cana-de-açúcar (2006), p. 133. , CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 25., 2006. Brasília. Anais... BrasíliaPlese, L.P.M., Silva, C.L., Foloni, L.L., Previsão da distribuição ambiental dos herbicidas utilizados na cultura do milho (2006), p. 134. , CONGRESSO BRASILEIRO DA CIÊNCIA DAS PLANTAS DANINHAS, 25., 2006, Brasília. Anais... BrasíliaSilva, C.L., Previsão ambiental da distribuição dos pesticidas aplicados na cultura do arroz (2007) Pestic.: R. Ecotoxicol Meio Amb., 17 (1), pp. 75-86Tomlin, C.D.S., (2000) The Pesticide Manual, , Farnham: British Crop Protection CouncilYenigün, O., Sohtorik, D., Calculation with the level II fugacity model for selected organophosphorus insecticides (1995) Water Air Soil Poll., 84 (1), pp. 175-18
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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