1,591 research outputs found
Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus
No full textNucleotide sequences of 52 South African isolates of African horse sickness virus (AHSV) collected during 2004–2005 and including viruses of all nine AHSV serotypes, were used to design and develop a duplex real-time reverse transcription quantitative PCR (RT-PCR) assay targeting the VP7 (S8) and NS2 (S9) genes of AHSV. The assay was optimized for detection of AHSV in fresh and frozen blood of naturally infected horses. Assay performance was enhanced using random hexamers rather than gene-specific primers for RT, and with denaturation of double-stranded RNA in the presence of random hexamers. The assay was efficient with a linear range of at least five orders of magnitude. The analytical sensitivity of the assay was 132 copies of the target genes (4125 copies per ml of blood), and the assay was at least 10-fold more sensitive than virus isolation on BHK-21 cells. The assay was also highly specific because it did not detect related orbiviruses, such as bluetongue and equine encephalosis viruses.ID: S0166093410000893; M3: Article; Accession Number: S0166093410000893; Author: M. Quan (a, b, ⁎); Author: C.W. Lourens (a, b); Author: N.J. MacLachlan (c); Author: I.A. Gardner (d); Author: A.J. Guthrie (a); Affiliation: Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Keyword: African horse sickness virus; Keyword: Real-time quantitative RT-PCR; Keyword: VP7 gene; Keyword: NS2 gene; Keyword: Duplex; Number of Pages: 8; Language: English
Co-curing bonding of carbon fibre/epoxy composite joints with excellent structure integrity using carbon fibre/PEEK tapes
No full textA novel co-curing process was proposed for the bonding of carbon fibre/epoxy composites by replacing traditional epoxy adhesives with carbon fibre/PEEK (CF/PEEK) tapes, with an attempt to improve the structure integrity. The lap-shear strengths, fatigue resistance and mode-I and mode-II fracture behaviour of the co-cured joints at 22 °C and 130 °C were investigated, and the failure mechanisms were also studied. The experimental results demonstrated that, by replacing an aerospace structural adhesive with surface-treated CF/PEEK tapes for the co-curing bonding of composite joints, the lap-shear strength of the joints had been increased by 47% and 68% at 22 °C and 130 °C, respectively; the fatigue life had been extended by 3.39 times; the mode-I fracture energy had been increased by 70% and 182% at 22 °C and 130 °C, respectively; and the mode-II fracture energy had been increased by 59% and 54% at 22 °C and 130 °C, respectively. An analysis on the failure surfaces of the tested specimens proved significant plastic deformation and breakage of the PEEK resin and extensive carbon fibre delamination being the main failure mechanisms of the CF/PEEK bonded joints. Overall, this study demonstrated a huge potential of replacing traditional film adhesives with CF/PEEK tapes for the co-curing bonding of aerospace composite joints with significantly enhanced structure integrity and thermal stability.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Structural Integrity & Composite
Affidavit of Florence Scrivner Toye re: transfer of Lease D, Carson Estate Company to Quan Bros., February 25, 1943
No full textDescribes transfer of Lease D with the Carson Estate Company from Florence Scrivner Toye to the Quan Bros. company; Quan Him Wong, George G. Quan. Signatures representing Florence Scrivner Toye, Harry G. Toye, Quan Him Wong, George G. Quan and Hamilton H. Cotton of the Carson Estate Company are included
[Affidavit] of Florence Scrivner Toye re: transfer of Lease D to Quan Bros., Carson Estate Company, January 6, 1943
No full textDescribes transfer of Lease D with the Carson Estate Company from Florence Scrivner Toye to the Quan Bros. company; Quan Him Wong, George G. Quan. No signatures on this document
Leasing Options
No full textArticle: Leasing Options
Author: Vuong Quan Hoang
Vietnam Economic Times (pg. 28)
Published: 15 October 199
Private Sector Eyes Investment Funds
No full textPrivate Sector Eyes Investment Funds
Author: Vuong Quan Hoang
Vietnam Investment Review (pg. 17)
15 - 21 June 199
Letter from [Minna A. Newman], Carson Estate Company to Mr. George G. Quan, February 25, 1943
No full textRefers to the transfer of Lease D from Florence Scrivner Toye to Quan Bros. Related affidavit is Item csudh_rsp_0551
Mode-II fracture behaviour of aerospace-grade carbon fibre/epoxy composites interleaved with thermoplastic veils
No full textThermoplastic veils based on Polyethylene-terephthalate (PET), Polyphenylene-sulfide (PPS) and Polyamide-12 (PA) fibres (∼10μm in diameter) were used to interlay unidirectional (UD), non-crimp fabric (NCF) and 5-Harness satin weave (5H) carbon fibre laminates. The PET and PPS veils remained in a fibrous form and the PA veils melted during the laminate curing process. The results of an end-loaded split test demonstrated significant improvements in the mode-II fracture performance in all cases. In general, interlaying thermoplastic veils was most efficient for toughening the UD laminates, with reduced improvements observed for the 5H and NCF laminates, respectively. The main toughening mechanism of the intact PET and PPS veils was thermoplastic fibre bridging. The melted PA veils mainly improved the fracture toughness of the epoxy at the mid-plane. The different toughening mechanisms of the veils, combined with different fracture mechanisms between the UD, NCF and 5H laminates, resulted in significantly different toughening levels
Transition metal promoted/catalyzed cage C-H/B-H functionalization of o-carboranes
No full textQuan, Yangjian.Thesis Ph.D. Chinese University of Hong Kong 2015.Includes bibliographical references (leaves 155-169).Abstracts also in Chinese.Title from PDF title page (viewed on 05, January, 2017)
Building Creative Competence in Globally Distributed Courses through Design Thinking
No full textHelping students think creatively is consistently cited as one of the key goals of education. Yet, across universities around the world, alarms have been sounding off suggesting that students are not prepared for a world where they are expected to solve messy, unstructured problems that don't have easy answers. This paper introduces design thinking, a human-centered innovation methodology that has been implemented in a design innovation program at Stanford University as well as at one of the most successful design consultancies. After a brief overview of design thinking, the author illustrates the key elements of this innovation pedagogy through its implementation at a university in Colombia. Realizing the potential of this methodology for building creative competence and confidence among students from all disciplines, and recognizing the power of the next generation of information and collaboration technologies and social media, the author proposes new research and development projects that will bring more creativity to traditional distance and blended learning programs through an infusion of design thinking
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