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Anisotropic fluorescence emission and photobleaching at the surface of one-dimensional photonic crystals sustaining bloch surface waves. I. theory
Full text linkPhotonic crystal (PC) enhanced fluorescence has been proposed as a novel tool for early disease detection in a liquid biopsy format. However, photobleaching of the emitters has never been deeply investigated, although its cross section is expected to increase due to the large field intensity enhancement. Herein, we report on a comprehensive theoretical description of the stationary fluorescence emission of molecular emitters bound to the surface of a one-dimensional photonic crystal (1DPC) biosensor. The model considers coupling of the emission to the large local density of the states provided by the 1DPC, in particular to the Bloch surface waves, which can be characterized by different polarization states. The rotational diffusion equation in the presence of photobleaching was solved analytically by a Laplace spherical harmonics analytical approach. The results show that photobleaching can severely affect the fluorescence emission in terms of total intensity and polarization composition, suggesting that a careful analysis of fluorescence anisotropy in biosensing experiments with PC should be carried out. We applied the model to some case of study conditions that take place in the experiments and propose a procedure to rule out the contribution of photobleaching. Last but not least, we propose fluorescence recovery after orientational photobleaching as a new tool to study the rotational diffusion of emitters, or labelled proteins, bound at a surface
commentario al testo unico delle leggi in materia bancaria e creditizia
No full textcommento al d.lgs. 385/1993 e successive modifich
Does ethic rating decrease firms’ Cost of Capital? Empirical insights from the Italian setting
No full textExperimentaLab: A virtual platform to enhance entrepreneurial education through training
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Anisotropic fluorescence emission and photobleaching at the surface of one-dimensional photonic crystals sustaining bloch surface waves. II. experiments
No full textPhotonic crystal (PC)-enhanced fluorescence has been proposed as a novel tool for early disease detection in liquid biopsy. Photobleaching of the emitters has never been deeply investigated, although its cross section is expected to increase because of the large field intensity enhancement in PC. Herein, we report on the experimental investigation of the anisotropic effects arising when fluorescence excitation and emission are coupled to differently polarized modes of the same PC structure. In particular, we experimentally characterized the anisotropic photobleaching taking place during the operation of one-dimensional PC (1DPC) biosensors sustaining Bloch surface waves (BSW) at their truncation edge. The experimental results are compared to the description provided by our theoretical model reported in a twin article. We demonstrate experimentally that photobleaching affects the response of a cancer biomarkers' detection assay and propose a procedure to compensate for it. Moreover, we describe the experimental use of BSW-coupled fluorescence and of the strong localization of the BSW at the 1DPC surface to selectively probe the rotational diffusion kinetics of proteins bound at the 1DPC surface with high spatial resolution and for different binding strengths
PARTIAL LEAST SQUARE PATH MODELLING AND MAXIMUM ENTROPY FOR THE STUDY OF ENTREPRENEURSHIP EDUCATION IN NAPLES UNIVERSITY
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