30 research outputs found
sj-docx-1-jom-10.1177_01492063231224353 – Supplemental material for Reputation-Damaging Events Over a Long Time Horizon: An Event-System Model of Substantive Reputation Repair
No full textSupplemental material, sj-docx-1-jom-10.1177_01492063231224353 for Reputation-Damaging Events Over a Long Time Horizon: An Event-System Model of Substantive Reputation Repair by Jarrod P. Vassallo, Yeonji Seo and Shahzad (Shaz) Ansari in Journal of Management</p
Abundance of the Membrane Proteome in Yeast Cells Lacking Spc1, a Non-catalytic Subunit of the Signal Peptidase Complex
No full textThe signal peptidase complex (SPC) mediates processing of signal peptides of secretory precursors. But, recent studies show that the eukaryotic SPC also cleaves internal transmembrane segments of some membrane proteins, and its non-catalytic subunit, Spc1/SPCS1 plays a critical role in this process. To assess the impact of Spc1 on membrane proteostasis, we carried out quantitative proteomics of yeast cells with and without Spc1. Our data show that the abundance of the membrane proteome in yeast cells lacking Spc1 is in general reduced compared to that in wild-type cells, implicating its role in controlling the cellular levels of membrane proteins.N
Oxidatively Modified Protein-Disulfide Isomerase?Associated 3 Promotes Dyskerin Pseudouridine Synthase 1?Mediated Malignancy and Survival of Hepatocellular Carcinoma Cells
No full textDyskerin pseudouridine synthase 1 (DKC1) is a conserved gene encoding the RNA-binding protein dyskerin, which is an essential component of the telomerase holoenzyme. DKC1 up-regulation is frequently observed in many different human cancers including hepatocellular carcinoma (HCC); however, its regulatory mechanisms remain unclear. Thus, we investigated the regulatory mechanism of DKC1 in HCC progression. We found that protein-disulfide isomerase-associated 3 (PDIA3) interacted with the DKC1 regulatory DNA in HCC cells but not in HCC cells with elevated reactive oxygen species (ROS) levels, using liquid chromatographic?tandem mass spectrometric analysis after isolating the DKC1 regulatory region binding proteins. PDIA3 repressed DKC1 expression in HCC cells by recognizing the G-quadruplex DNA at the DKC1 location. However, oxidative modification of PDIA3 induced by ROS redistributed this protein into the cytosolic regions, which stimulated DKC1 expression. We also identified Met338 in PDIA3 as the oxidatively modified residue and validated the effect of oxidative modification using an ectopic expression system, a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 knock-in system, and a xenograft mouse model. We observed that oxidatively modified PDIA3 promoted DKC1-mediated malignancy and survival of HCC cells in vitro and in vivo. HCC tissues showed a positive association with ROS, cytoplasmic PDIA3, and nuclear DKC1 levels. HCC patients with high PDIA3 protein and DKC1 mRNA levels also displayed reduced recurrence-free survival rates. Cumulatively, the results showed that cytoplasmic PDIA3 activity could be essential in raising DKC1 expression in HCC progression and predicting poor prognoses in HCC patients. Conclusion: Our study indicates that the elevated ROS levels in HCC modulate cytoplasmic PDIA3 levels, resulting in HCC cell survival through DKC1 up-regulation. (c) 2018 by the American Association for the Study of Liver Diseases11sciescopu
Angle-resolved photoemission spectroscopy study of a system with a double charge density wave transition: ErTe3
No full textThe electronic structures of ErTe3, having double charge density wave (CDW) transitions, have been investigated by performing polarization- and temperature (T)-dependent angle-resolved photoemission spectroscopy (ARPES) and Er 4d -> 4f resonant PES measurements for high-quality single crystals. It is found that Er 4 f electrons do not contribute directly to CDW formation. The shadow Fermi surfaces (FSs) are clearly observed, arising from band folding due to the interlayer interaction. Twofold symmetric FS, but with weak FS reconstruction, is observed in the CDW phase of ErTe3, which is similar to that of PrTe3 having the CDW modulation vector Q approximate to 5/7 c* approximate to 5/7 a*. Very good agreement is found between the constant energy maps and ARPES measured in the CDWphase and the density functional theory band calculation for the non-CDW phase, implying very weak and only partial CDW-induced FS reconstruction in ErTe3. Very weak and similar circular dichroism is observed along different symmetry lines in both FS and ARPES, suggesting weak chiral orbital and spin polarizations in the Te 5p orbitals. T-dependent FS and ARPES measurements reveal closing of the CDW gap on heating but no clear changes across T-CDW2 and T-CDW1, supporting the very weak CDW distortion in ErTe3. The origin of the very weak CDW reconstruction in ErTe3 is likely to be the small ionic sizes of Er ions and the concomitant small volume that give rise to the imperfect FS nesting and the reduced density of states at the Fermi level. The very weak FS reconstruction at the first CDW transition along c* is considered to be a reason why there exists a second CDW transition further along a*, which elucidates the observed double CDW transitions in ErTe3.11Nsciescopu
Spc1 regulates the signal peptidase-mediated processing of membrane proteins
No full text© 2021. Published by The Company of Biologists Ltd.Signal peptidase (SPase) cleaves the signal sequences (SSs) of secretory precursors. It contains an evolutionarily conserved membrane protein subunit, Spc1, that is dispensable for the catalytic activity of SPase and whose role remains unknown. In this study, we investigated the function of yeast Spc1. First, we set up an in vivo SPase cleavage assay using variants of the secretory protein carboxypeptidase Y (CPY) with SSs modified in the N-terminal and hydrophobic core regions. When comparing the SS cleavage efficiencies of these variants in cells with or without Spc1, we found that signal-anchored sequences became more susceptible to cleavage by SPase without Spc1. Furthermore, SPase-mediated processing of model membrane proteins was enhanced in the absence of Spc1 and was reduced upon overexpression of Spc1. Spc1 co-immunoprecipitated with proteins carrying uncleaved signal-anchored or transmembrane (TM) segments. Taken together, these results suggest that Spc1 protects TM segments from SPase action, thereby sharpening SPase substrate selection and acting as a negative regulator of the SPase-mediated processing of membrane proteins.11Nsciescopu
Statistical properties of COVID-19 transmission intervals in Republic of Korea
No full textA transmission interval for an infectious disease is important to understand epidemic processes in complex networks. The transmission interval is defined as a time interval between one person\u27s infection and their infection to another person. To study statistical properties of transmission intervals, we analyze a COVID-19 dataset of confirmed cases in Republic of Korea that has been collected for two years since the confirmation of the first case on 19 January 2020. Utilizing demographic information of confirmed individuals, such as sex, age, residence location, and the nature of relation between infectors and infectees, we find that transmission intervals are rarely affected by sexes, but they tend to have larger values for the youngest and oldest age groups than other groups. We also find some metropolitan cities or provinces with relatively larger (smaller) transmission intervals than other locations. These empirical findings might help us to better understand dynamical mechanisms of epidemic processes in complex social systems.7 pages, 6 figure
Effect of Hearing Rehabilitation Therapy Program in Hearing Aid Users: A Prospective Randomized Controlled Study
No full textOBJECTIVES: Despite sufficient hearing gains, many patients with hearing loss have difficulty using hearing aids due to poor word recognition ability. This study was performed to introduce our hearing rehabilitation therapy (HRT) program for hearing aid users and to evaluate its effect on hearing improvement. METHODS: In this prospective randomized case-control study, 37 participants with moderate or moderate-severe sensorineural hearing loss who had used bilateral hearing aids for more than 3 months with sufficient functional hearing gain were enrolled in this study. Nineteen participants were randomly assigned to the control group (CG) and 18 patients were assigned to participate in our HRT program once a week for 8 consecutive weeks (hearing rehabilitation therapy group [HRTG]). Their hearing results and questionnaire scores for hearing handicap and hearing aid outcomes were prospectively collected and compared between the two groups. RESULTS: After completing 8 weeks of the HRT program, the HRTG showed a significantly greater improvement in scores for consonant-only and consonant-vowel sound perception than the CG (P<0.05). In addition, the HRTG showed a significant improvement in hearing ability as measured by two questionnaires (P<0.05), while no differences were observed in the CG. However, word and sentence recognition test results did not show significant differences between the two groups. CONCLUSION: Even after short-term HRT, patients had subjectively better hearing outcomes and improved phoneme perception ability; this provides scientific evidence regarding a possible positive role for HRT programs in hearing aid users. Further validation in a larger population through a long-term follow-up study is needed
Protohyale (Boreohyale) triangulata Hiwatari 2003
No full textProtohyale (Boreohyale) triangulata (Hiwatari, 2003) (Korean Name: Se-mo-son-hae-jo-sum-i-yeop-sae-u) Hyale schmidti.— Iwasa, 1939: 278, Pl. 17, fig. 17. Hyale triangulata Hiwatari, 2003: 245, figs 10–11. Hyale rubra.— Kim & Kim, 1987: 16, fig. 14. Protohyale (Boreohyale) triangulata.— Jung & Yoon, 2013: 198, figs 1–4. Material examined. 2 Ƌ 2 ♀, Cheongsando Is., Wando-gun, 23 May 1998, (Y. Eun); 15 Ƌ, Bijindo Is., Tongyeong-si, 8 July 1998, (I.S. Seo); 5 Ƌ 3 ♀, Noryang-ri, Hadong-gun, 30 May 1999, (Y. Eun); 1 Ƌ 8 ♀, Bigeumdo Is., Sinan-gun, 21 July 2000, (Y. Eun); 2 Ƌ, Magdo Is., Yokji-myeon, 18 July 2001, (Y. Eun); 5 Ƌ 4 ♀, Patseom Is., Goseong-gun, 26 June 2002, (Y.H. Kim); 3 Ƌ 2 ♀, Yeonji-ri, Uljin-gun, 9 July 2002, (Y.H. Kim); 4 Ƌ 5 ♀, Mallipo beach, Taean-gun, 25 September 2003, (Y.H. Kim); 8 Ƌ 17 ♀, Gamdo Is., Yeosu-si, 21 March 2004, (T.S. Park); 30 Ƌ 30 ♀, Anmyeon beach, Taean-gun, 2 April 2004, (T.S. Park); 11 Ƌ 13 ♀, Namae-ri, Yangyanggun, 26 February 2005, (Y.H. Kim); 1 Ƌ 6 ♀, Homigot, Pohang-si, 26 August 2005, (Y. Eun); 1 Ƌ 3 ♀, Uido Is., Sinan-gun, 5 October 2005, (Y. Eun); 4 Ƌ 7 ♀, Jangsaengpo-dong, Ulsan-si, 9 January 2008, (Y.H. Kim); 6 Ƌ 2 ♀, Naemaemuldo Is., Goheung-gun, 23 June 2008, (S.S. Hong); 7 Ƌ 7 ♀, Namaehang, Yangyang-gun, 7 May 2011, (Y. Eun); 3 Ƌ 62 ♀, Hyeopjae beach, Jeju-si, 12 July 2012, (Y. Eun); 3 Ƌ 1 ♀, Kimnyeong beach, Jeju-si, 28 March 2013, (E.J. Kim, S. Lee & S.K. Lee); 5 Ƌ 3 ♀, Jeongdo-ri, Wando-gun, 3 September 2013, (Y. Eun, S. Lee). Previous Korean records. Wando-gun, Seocheon-gun. (Jung & Yoon, 2013). Diagnosis. Gnathopod 1, propodus triangular in form, without large posterior cusp, posteroproximal corner subquadrate. Gnathopod 2, propodus subovate, palm not oblique, slightly shorter than posterior margin. Distribution. Korea, Japan.Published as part of Eun, Ye, Hendrycks, Ed A. & Kim, Young-Hyo, 2016, The family Hyalidae (Crustacea: Amphipoda: Talitroidea) from Korean waters. 2. Genus Protohyale Bousfield & Hendrycks, 2002, pp. 231-248 in Zootaxa 4175 (3) on page 247, DOI: 10.11646/zootaxa.4175.3.3, http://zenodo.org/record/16064
Escape from Hell-Joseon: A Study of Korean Long-term Travelers in India
No full textThis article was originally published in 2016 in 『비교문화연구』 [Cross-cultural studies]
22(2): 291-328; Translated into English by Yeonji Ghim.
* This article is based on the authors M.A. thesis by significantly modifying chapters 1, 3,
5, and adding new discussions. The author would like to express sincere gratitude to the
three examiners who provided instructive comments for this article.The objectives of this study were: 1) to understand present day long-term travels of Korean youth by connecting these to a Hell- Joseon discourse; 2) to examine the Korean phenomenon of tal-Joseon (escape from Korea), a practice that is difficult to explain through concepts related to tourism or immigration; 3) to identify the life changes travelers experience after tal-Joseon travel with a focus on their increased mobility. The results of this research are the following. First, people, mostly youths, who are frustrated by Koreas social structure and culture are traveling abroad for extended periods to get away from Korea. Second, the migration routes tal-Joseon travelers take are different depending on the travelers social class. Lower-middle class youth travelers, for example, follow a repetitive pattern of physical labor in developed nations and long-term rest in developing nations. Third, the main goal sought by long-term travelers in India, one of most popular destinations for long-term rest, is detoxing from Hell-Joseon through rest, healing, and a search for the self. Fourth, when long-term travelers finish their trips, the meaning of home changes for them; an increased mobility is observed in their lives. The tal-Joseon travel phenomenon is related to an increasing global mobility, a trend of blurring boundaries between tourism and migration, and the structural problems in Korea where young people are increasingly excluded from society including the labor market. This study contributes to reflective research on those who choose to run away from Korean society and contributes to a new theory of global mobility
