1,787 research outputs found
Eli Nichols letter to Benjamin Lundy, March 17th, 1839
No full textFriendly note from Eli Nichols to Benjamin Lundy covering topics in contemporary abolition, ranging from the social status of abolitionists to the oppression of the poor. Much of the letter concerns a review of contemporary social movements in equality-based education, including Shaker and Quaker communities. The letter concludes in discussion of Nichols' and Lundy's interest in forming a freed slave colony or community in then-Mexico, and describes the climate and culture of those regions in detail. Benjamin Lundy (1789-1839) was a prominent Quaker abolitionist best known for his development of abolitionist periodicals. His Genius of Universal Emancipation was first published in 1821 from his home in Mt. Pleasant, Ohio, and enjoyed a wide circulation across the antebellum United States. In the 1820s, the young William Lloyd Garrison came to work for The Genius. Benjamin Lundy traveled widely seeking subscriptions to The Genius, giving talks about the anti-slavery movement, and observing and documenting the conditions of enslaved people across the Americas. He was also involved in the establishment of freed slave colonies in Mexico
Interview with Eli Rosenbaum
No full textFor transcript, click the Download button above. For video index, click the link below.
Eli M. Rosenbaum (WG\u2777) served as director of the U.S. DOJ Office of Special Investigations, which was primarily responsible for identifying, denaturalizing, and deporting Nazi war criminals, from 1994 to 2010, when the office was merged into the new Human Rights and Special Prosecution Section. He is now the Director of Human Rights Enforcement Strategy and Policy in the new Department of Justice section. He is the primary author of Betrayal: The Untold Story of the Kurt Waldheim Investigation, which narrates the inquiry he led into Waldheim\u27s involvement in Nazi war crimes. In 1997 he was named an honorary fellow of Penn Law School
Les petits ARN non codants du spermatozoïde bovin : de potentiels biomarqueurs de la fertilité mâle ?
No full textMost dairy cows are inseminated and sub-fertile bulls can have an overall dramatic negative impact on the sustainability of the dairy sector. The bull fertility prediction, through efficient quality control (QC) procedures is thus crucial for the cattle breeding sector. Over the last decades, several biomarkers and quality control procedures have been proposed to guarantee semen fertility, including assessment of key functional parameters using flow cytometry. However, their relevance for routine QC has yet to be ascertained and their predictive value is too limited to confidently discard subfertile bulls. Thus, studies have been conducted by LabCom SeQuaMol to explore to what extent the bull sperm epigenome may be relevant to improve fertility prediction. In this respect, this PhD thesis was focused on small noncoding RNAs (sncRNAs), which have been shown to play a role in spermatogenesis and fertilization. We aimed at improving the scientific knowledge on bull sperm sncRNA content in order to develop a routine tool to predict bull fertility. The work has been organized according to three main themes: providing a comprehensive overview of cattle sperm sncRNA, studying their expression dynamics during sperm maturation along epididymis and identifying biomarkers associated with bull fertility.L’utilisation de taureaux subfertiles entraîne des pertes de production, une augmentation du taux de réforme des vaches pour infertilité et un gaspillage des ressources. C’est pourquoi le contrôle qualité de la semence utilisée en Insémination Animale est une préoccupation majeure des entreprises de sélection. Divers protocoles de contrôle qualité ont été développés au cours des dernières décennies, exploitant des biomarqueurs et des paramètres fonctionnels clés mesurés par cytométrie en flux. Toutefois, la capacité prédictive de ces tests reste limitée et ne permet pas d’identifier les taureaux subfertiles. C’est pourquoi des travaux ont été entrepris dans le cadre du LabCom SeQuaMol, afin d’explorer l’intérêt de l’épigénome spermatique comme biomarqueur de fertilité chez les taureaux. Ainsi, les travaux menés au cours de cette thèse ont portés sur les petits ARNs non codants (sncRNA), dont le rôle au cours de la spermatogénèse et de la fécondation a été suggéré et démontré. L’amélioration des connaissances sur le contenu en sncRNA du spermatozoïde bovin éjaculé avait comme objectif pratique de développer un outil permettant de fiabiliser la prédiction en routine de la fertilité des taureaux. Les travaux ont été organisés selon trois axes : l’établissement du contenu exhaustif en sncRNA du sperme bovin éjaculé, l’étude de la dynamique d’expression de ces sncRNAs au cours de la maturation épididymaire et l'identification in fine de biomarqueurs associés à la fertilité des taureaux
Predicting Male Fertility: From Decades of Semen Analysis to Future Perspectives
No full textInternational audienc
Les petits ARN non codants du spermatozoïde bovin : de potentiels biomarqueurs de la fertilité mâle ?
No full textMost dairy cows are inseminated and sub-fertile bulls can have an overall dramatic negative impact on the sustainability of the dairy sector. The bull fertility prediction, through efficient quality control (QC) procedures is thus crucial for the cattle breeding sector. Over the last decades, several biomarkers and quality control procedures have been proposed to guarantee semen fertility, including assessment of key functional parameters using flow cytometry. However, their relevance for routine QC has yet to be ascertained and their predictive value is too limited to confidently discard subfertile bulls. Thus, studies have been conducted by LabCom SeQuaMol to explore to what extent the bull sperm epigenome may be relevant to improve fertility prediction. In this respect, this PhD thesis was focused on small noncoding RNAs (sncRNAs), which have been shown to play a role in spermatogenesis and fertilization. We aimed at improving the scientific knowledge on bull sperm sncRNA content in order to develop a routine tool to predict bull fertility. The work has been organized according to three main themes: providing a comprehensive overview of cattle sperm sncRNA, studying their expression dynamics during sperm maturation along epididymis and identifying biomarkers associated with bull fertility.L’utilisation de taureaux subfertiles entraîne des pertes de production, une augmentation du taux de réforme des vaches pour infertilité et un gaspillage des ressources. C’est pourquoi le contrôle qualité de la semence utilisée en Insémination Animale est une préoccupation majeure des entreprises de sélection. Divers protocoles de contrôle qualité ont été développés au cours des dernières décennies, exploitant des biomarqueurs et des paramètres fonctionnels clés mesurés par cytométrie en flux. Toutefois, la capacité prédictive de ces tests reste limitée et ne permet pas d’identifier les taureaux subfertiles. C’est pourquoi des travaux ont été entrepris dans le cadre du LabCom SeQuaMol, afin d’explorer l’intérêt de l’épigénome spermatique comme biomarqueur de fertilité chez les taureaux. Ainsi, les travaux menés au cours de cette thèse ont portés sur les petits ARNs non codants (sncRNA), dont le rôle au cours de la spermatogénèse et de la fécondation a été suggéré et démontré. L’amélioration des connaissances sur le contenu en sncRNA du spermatozoïde bovin éjaculé avait comme objectif pratique de développer un outil permettant de fiabiliser la prédiction en routine de la fertilité des taureaux. Les travaux ont été organisés selon trois axes : l’établissement du contenu exhaustif en sncRNA du sperme bovin éjaculé, l’étude de la dynamique d’expression de ces sncRNAs au cours de la maturation épididymaire et l'identification in fine de biomarqueurs associés à la fertilité des taureaux
Les petits ARN non codants du spermatozoïde bovin : de potentiels biomarqueurs de la fertilité mâle ?
No full textMost dairy cows are inseminated and sub-fertile bulls can have an overall dramatic negative impact on the sustainability of the dairy sector. The bull fertility prediction, through efficient quality control (QC) procedures is thus crucial for the cattle breeding sector. Over the last decades, several biomarkers and quality control procedures have been proposed to guarantee semen fertility, including assessment of key functional parameters using flow cytometry. However, their relevance for routine QC has yet to be ascertained and their predictive value is too limited to confidently discard subfertile bulls. Thus, studies have been conducted by LabCom SeQuaMol to explore to what extent the bull sperm epigenome may be relevant to improve fertility prediction. In this respect, this PhD thesis was focused on small noncoding RNAs (sncRNAs), which have been shown to play a role in spermatogenesis and fertilization. We aimed at improving the scientific knowledge on bull sperm sncRNA content in order to develop a routine tool to predict bull fertility. The work has been organized according to three main themes: providing a comprehensive overview of cattle sperm sncRNA, studying their expression dynamics during sperm maturation along epididymis and identifying biomarkers associated with bull fertility.L’utilisation de taureaux subfertiles entraîne des pertes de production, une augmentation du taux de réforme des vaches pour infertilité et un gaspillage des ressources. C’est pourquoi le contrôle qualité de la semence utilisée en Insémination Animale est une préoccupation majeure des entreprises de sélection. Divers protocoles de contrôle qualité ont été développés au cours des dernières décennies, exploitant des biomarqueurs et des paramètres fonctionnels clés mesurés par cytométrie en flux. Toutefois, la capacité prédictive de ces tests reste limitée et ne permet pas d’identifier les taureaux subfertiles. C’est pourquoi des travaux ont été entrepris dans le cadre du LabCom SeQuaMol, afin d’explorer l’intérêt de l’épigénome spermatique comme biomarqueur de fertilité chez les taureaux. Ainsi, les travaux menés au cours de cette thèse ont portés sur les petits ARNs non codants (sncRNA), dont le rôle au cours de la spermatogénèse et de la fécondation a été suggéré et démontré. L’amélioration des connaissances sur le contenu en sncRNA du spermatozoïde bovin éjaculé avait comme objectif pratique de développer un outil permettant de fiabiliser la prédiction en routine de la fertilité des taureaux. Les travaux ont été organisés selon trois axes : l’établissement du contenu exhaustif en sncRNA du sperme bovin éjaculé, l’étude de la dynamique d’expression de ces sncRNAs au cours de la maturation épididymaire et l'identification in fine de biomarqueurs associés à la fertilité des taureaux
Bovine sperm sncRNAs vary according to bull fertility
No full textInternational audienceSemen fertility remains a key concern in the breeding sector. As conventional sperm quality assessments fail to accurately predict fertility, sperm small non coding RNAs (sncRNAs) content are emerging as a promising approach for fertility issues. Ejaculates from 100 Montbéliard bulls (43 subfertiles vs 57 fertiles according to their adjusted non-return rate at 56 days) were analysed to identify potential sperm sncRNAs fertility biomarkers. These ejaculates were collected between 17 and 19 months of age from bulls born from 2011 to 2015. Extracted RNAs have been used for UMI sequencing (Illumina HISeq2000, 20 million reads, 75 bases single-end). MiRDeep2 was used for annotation and quantification, while DeSeq2 identified differentially expressed sncRNAs between fertility groups. Expression differences were considered significant at Benjamini-Hochberg adjusted p-value <0.05 and differential at <0.1. TargetScan was used to predict the genes potentially targeted by the identified microRNAs(miRNAs). Among the 413,196 analysed sncRNAs, 94% are expressed under 10 copies per sample, nearly 5% between 5 to 100 copies, and less than 1% above 100 copies. The twenty most expressed sncRNAs, mainly miRNAs and transfer RNA fragments (tsRNAs), represent 33% of total reads. A total of 552 differential sncRNAs were considered by DeSeq2, with 151 showing a significant differential expression. Interestingly, 49.6% of these sequences were acquired in the epididymis cauda. Several differentially expressed miRNAs, such as miR-6526 or miR-30d, have already been associated with sperm motility or abnormalities. Enriched biological pathways among predicted target genes include anatomical structure morphogenesis, system development and regulation of transcription. Some of these genes, such as STAT3 or SMAD1, play established roles in embryo development.These interesting findings indicate that specific sperm sncRNAs are associated with fertility status. Further studies are necessary to confirm their potential as biomarkers for bull fertility
Bovine sperm sncRNAs vary according to bull fertility
No full textInternational audienceSemen fertility remains a key concern in the breeding sector. As conventional sperm quality assessments fail to accurately predict fertility, sperm small non coding RNAs (sncRNAs) content are emerging as a promising approach for fertility issues.</div
Students And Eli Weisel at the 1993 Commencement Ceremony
No full textNobel-prize winning author, Eli Weisel, at the 1993 Commencement Ceremony, with students
How the ELI assists GAs in Their Professional Development
No full textThis report demonstrates how ELI supports teachers’ professional development. First, the report illustrates teachers’ duties throughout the academic semester such as classroom observation and curriculum area meeting. Further, it demonstrates diverse aspects of being an ELI teacher as part of Graduate Assistantship (GA), including file sharing, office space, professional development assignments, and administrative supports. In the end, the author reflects personal experience of being an ELI teacher
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