58 research outputs found
Warmer winters result in reshaping of the European beech forest soil microbiome (bacteria, archaea and fungi) - With potential implications for ecosystem functioning
No full textIn temperate regions, climate warming alters temperature and precipitation regimes. During winter, a decline in insulating snow cover changes the soil environment, where especially frost exposure can have severe implications for soil microorganisms and subsequently for soil nutrient dynamics. Here, we investigated winter climate change responses in European beech forests soil microbiome. Nine study sites with each three treatments (snow exclusion, insolation, and ambient) were investigated. Long-term adaptation to average climate was explored by comparing across sites. Triplicated treatment plots were used to evaluate short-term (one single winter) responses. Community profiles of bacteria, archaea and fungi were created using amplicon sequencing. Correlations between the microbiome, vegetation and soil physicochemical properties were found. We identify core members of the forest-microbiome and link them to key processes, for example, mycorrhizal symbiont and specialized beech wood degraders (fungi) and nitrogen cycling (bacteria, archaea). For bacteria, the shift of the microbiome composition due to short-term soil temperature manipulations in winter was similar to the community differences observed between long-term relatively cold to warm conditions. The results suggest a strong link between the changes in the microbiomes and changes in environmental processes, for example, nitrogen dynamics, driven by variations in winter climate
RoboFrame - Softwareframework für mobile autonome Robotersysteme
No full textDas Fachgebiet Simulation und Systemoptimierung der Technischen Universität Darmstadt beschäftigt sich mit der Forschung an mobilen autonomen Robotersystemen. Neben der Hardware und robusten Algorithmen zur Robotersteuerung kommt auch der zugrunde liegenden Softwarearchitektur eine zunehmend wichtige Bedeutung zu. In dieser Arbeit wurde ein plattformunabhängiges Framework entwickelt, mit dessen Hilfe eine Robotersteuerung und eine graphische Oberfläche zu Entwicklungsund Debugzwecken entworfen werden kann. Als beispielhafte Implementierung wurden zusammen mit Praktikumsgruppen zwei Anwendungen entwickelt, die im Frühjahr 2005 an der Internationalen RoboCup GermanOpen und im Sommer 2005 an der RoboCup Weltmeisterschaft in der Humanoid League erfolgreich teilnahmen. Abstract The Simulation and Systems Optimization Group of the Department of Computer Science of the Technische Universität Darmstadt concerns itself with the research of mobile autonomous robotic systems. Apart from the hardware and durable algorithms for robotic control the basic software architecture becomes more and more important. In this work a platform-independent framework was developed, which assists to implement a robot control system and a graphic surface for assisting in the development process and for debugging purposes. Two example application were implemented based on that framework together with groups of practical courses, which participated successfully in the Humanoid League at the International RoboCup GermanOpen in spring 2005 and at the RoboCup World championship in summer 2005
La publicité « testimoniale » : entre exemplification et exemplarité
No full textL’article analyse la relation entre la notion de témoignage et la publicité, en s’attachant aux messages qualifiés de « testimoniaux ». Par l’examen détaillé de deux corpus constitués de publicités audiovisuelles, l’auteure interroge la définition du testimonial ainsi que ses différents usages dans le discours marchand. De cette analyse ressortent deux fonctions majeures du testimonial publicitaire : l’exemplification et l’exemplarité. L’étude des procédures d’exemplification et d’exemplarité au sein des testimoniaux permet de présenter, en conclusion, des hypothèses tentant d’expliquer pourquoi une telle rhétorique du témoignage est toujours omniprésente dans la publicité contemporaine.This article inquires the relation between testimony notion and advertising, by focusing on the so-called “testimonial” messages. From a detailed examination of two corpora composed of audiovisual advertising, the author questions the testimonial definition and the various testimonial applications in the commercial discourse. From this analysis, two major testimonial functions emerge: exemplification and exemplariness. The study of these functions suggests some hypotheses attempting to explain why the testimonial rhetoric is still pervasive in the contemporary advertising; these hypotheses are exposed in the article’s conclusion
La publicité « testimoniale » : entre exemplification et exemplarité
No full textL’article analyse la relation entre la notion de témoignage et la publicité, en s’attachant aux messages qualifiés de « testimoniaux ». Par l’examen détaillé de deux corpus constitués de publicités audiovisuelles, l’auteure interroge la définition du testimonial ainsi que ses différents usages dans le discours marchand. De cette analyse ressortent deux fonctions majeures du testimonial publicitaire : l’exemplification et l’exemplarité. L’étude des procédures d’exemplification et d’exemplarité au sein des testimoniaux permet de présenter, en conclusion, des hypothèses tentant d’expliquer pourquoi une telle rhétorique du témoignage est toujours omniprésente dans la publicité contemporaine.This article inquires the relation between testimony notion and advertising, by focusing on the so-called “testimonial” messages. From a detailed examination of two corpora composed of audiovisual advertising, the author questions the testimonial definition and the various testimonial applications in the commercial discourse. From this analysis, two major testimonial functions emerge: exemplification and exemplariness. The study of these functions suggests some hypotheses attempting to explain why the testimonial rhetoric is still pervasive in the contemporary advertising; these hypotheses are exposed in the article’s conclusion
A language to simplify computation of differential mobility analyzer response functions
No full textA language for computation of differential mobility analyzer (DMA) response functions is introduced. The language consists of short programming language expressions that evaluate to the size distribution of particles exiting a DMA. The language permits application of the same framework to single and tandem DMA setups. Expressions are derived for calculation of the convolution matrix used in inversion of size distribution data, calculation of the convolution matrix for transit through tandem DMA systems, and calculation of the size and mobility distribution through DMA systems that involve one or multiple DMAs. The contribution of multiply charged particles to the total response distributions can be explicitly resolved. The derived convolution matrix is suitable for inverting scanning mobility particle sizer response functions using standard regularization techniques. Users can modify and substitute any of the convolution terms—comprising the DMA transfer function, detector efficiency, loss rate, and charging efficiencies—to express response functions of nonstandard DMA configurations. Example applications are presented, including classification of particle size, measurement of size-resolved cloud condensation nuclei activity, characterization of hygroscopicity and volatility tandem DMA response functions, and characterization of the dual tandem DMA system for dimer preparation. The source code and examples are shared as free software and are hosted on an online collaborative software development platform that allows for version tracking and crediting contributors. Adoption of the language may facilitate the design and optimization of custom-built systems that involve unique arrangements of DMAs and detectors. Copyright © 2018 The Author(s). Published with license by Taylor & Francis Group, LLC.</p
Total counts of datasets and software code, by author institutional affiliation.
No full textTotal counts of datasets and software code, by author institutional affiliation.</p
Multilevel testing of control software for teams of autonomous mobile robots
No full textDeveloping control software for teams of autonomous mobile robots is a challenging task, which can be facilitated using frameworks with ready to use components. But testing and debugging the resulting system as teached in modern software engineering to be free of errors and tolerant to sensor noise in a real world scenario is to a large extend beyond the scope of current approaches. In this paper multilevel testing strategies using the developed frameworks RoboFrame and MuRoSimF are presented. Testing incorporating automated tests, online and offline analysis and software-in-the-loop (SIL) tests in combination with real robot hardware or an adequate simulation are highly facilitated by the two frameworks. Thus the efficiency of validation of complex real world applications is improved. In this way potential errors can be identified early in the development process and error situations in real world operations can be reduced significantly
Modular software architecture for teams of cooperating, heterogeneous robots
No full textFor teams of cooperating autonomous lightweight robots with challenging dynamical locomotion properties a platform independent modular software architecture and platform independent modules for sensor data processing, planning and motion control have been developed. The software architecture allows high level communication between modules on different abstraction levels of the control architecture within one robot system as well as communication between different and heterogeneous robots and computers using wireless network. Very different behavior control paradigms may be realized on the basis of the developed architecture. The application to teams of cooperating small and medium size humanoid robots is investigated in this paper. Scenarios for inter robot communication and cooperative task accomplishment are described
The Chemically Synthesized Ageladine A-Derivative LysoGlow84 Stains Lysosomes in Viable Mammalian Brain Cells and Specific Structures in the Marine Flatworm Macrostomum lignano
No full textBased on the chemical structure and the known chemical synthesis of the marine sponge alkaloid ageladine A, we synthesized the ageladine A-derivative 4-(naphthalene-2-yl)-1H-imidazo[4,5-c]pyridine trifluoroacetate (LysoGlow84). The two-step synthesis started with the Pictet-Spengler reaction of histamine and naphthalene-2-carbaldehyde to
a tetrahydropyridine intermediate, which was dehydrogenated with activated manganese (IV) oxide to LysoGlow84. Structure and purity of the synthesized LysoGlow84 were confirmed by NMR spectroscopy and mass spectrometry. The fluorescence intensity emitted by LysoGlow84 depended strongly on the pH of the solvent with highest fluorescence intensity recorded at pH 4. The fluorescence maximum (at 315 nm excitation) was observed at 440 nm. Biocompatibility of LysoGlow84 was investigated using cultured rat brain astrocytes and the marine flatworm Macrostomum lignano. Exposure of the astrocytes for up to 6 h to micromolar concentrations of LysoGlow84 did not compromise cell viability, as demonstrated by several viability assays, but revealed a promising property of this compound for staining of cellular vesicles. Conventional fluorescence microscopy as well as confocal scanning microscopy of LysoGlow84-treated astrocytes revealed co-localization of LysoGlow84 fluorescence with that of LysoTracker® Red DND-99. LysoGlow84 stained unclear structures in Macrostomum lignano, which were identified as lysosomes by co-staining with LysoTracker. Strong fluorescence staining by LysoGlow84 was further observed around the worms’ anterior gut and the female genital pore which were not counterstained by LysoTracker Red. Thus, LysoGlow84 is a new promising dye that stains lysosomes and other acidic compartments in cultured cells and in worms
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