812 research outputs found
The cell cycle dependent phosphorylation of histone H3 is correlated with the condensation of plant mitotic chromosomes
No full textHouben, Andreas ; Wako, Toshiyuki ; Furushima‐shimogawara, Rieko ; Presting, Gernot ; Künzel, Gottfried ; Schubert, Ingo ; Fukui, Küch
Recommended from our members
A gradual depth-dependent change of connectivity features of supragranular pyramidal cells in rat barrel cortex
No full textRecent experimental evidence suggests a finer genetic, structural and functional subdivision of the layers which
form a cortical column. The classical layer II/III (LII/III) of rodent neocortex integrates ascending sensory information with contextual cortical information for behavioral read-out. We systematically investigated to which extent regular-spiking supragranular pyramidal neurons, located at different depths within the cortex,
show different input-output connectivity patterns. Combining glutamate-uncaging with whole-cell recordings
and biocytin filling, we revealed a novel cellular organization of LII/III: (i) “Lower LII/III” pyramidal cells receive a very strong excitatory input from lemniscal LIV and much fewer inputs from paralemniscal LVa. They project to all layers of the home column, including a feedback projection to LIV whereas transcolumnar projections are relatively sparse. (ii) “Upper LII/III” pyramidal cells also receive their strongest input from LIV, but in addition, a very strong and dense excitatory input from LVa. They project extensively to LII/III as well as LVa and Vb of their home and neighboring columns, (iii) “Middle LII/III” pyramidal cell show an intermediate connectivity phenotype that stands in many ways in-between the features described for lower versus upper LII/III. “Lower LII/III” intracolumnarly segregates and transcolumnarly integrates lemniscal information whereas “upper LII/III” seems to integrate lemniscal with paralemniscal information. This suggests a finegrained functional subdivision of the supragranular compartment containing multiple circuits without any obvious cytoarchitectonic, other structural or functional correlate of a laminar border in rodent barrel cortex
A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules.
No full textA major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants. The use of trinucleotides mixtures for controlled randomization results in superior library diversity and offers the ability to specify the type and distribution of the amino acids at each position. Here we describe the generation of a high diversity gene library using tHisF of the hyperthermophile Thermotoga maritima as a scaffold. Combining various rational criteria with contingency, we targeted 26 selected codons of the thisF gene sequence for randomization at a controlled level. We have developed a novel method of creating full-length gene libraries by combinatorial assembly of smaller sub-libraries. Full-length libraries of high diversity can easily be assembled on demand from smaller and much less diverse sub-libraries, which circumvent the notoriously troublesome long-term archivation and repeated proliferation of high diversity ensembles of phages or plasmids. We developed a generally applicable software tool for sequence analysis of mutated gene sequences that provides efficient assistance for analysis of library diversity. Finally, practical utility of the library was demonstrated in principle by assessment of the conformational stability of library members and isolating protein variants with HisF activity from it. Our approach integrates a number of features of nucleic acids synthetic chemistry, biochemistry and molecular genetics to a coherent, flexible and robust method of combinatorial gene synthesis
Ingo Plag, Word-Formation in English (2
No full text1. General observations Ingo Plag is Professor of English Linguistics at Heinrich-Heine-Universität Düsseldorf. He has published articles in specialized journals like Linguistics, Language or English Language and Linguistics and in works like the Yearbook of Morphology [2001], Word-Formation: An International Handbook of the Languages of Europe [2016] or Word Knowledge and Word Usage: A Cross-Disciplinary Guide to the Mental Lexicon [2017]. He is the author of Morphological Productivity: Stru..
Remarks on Early Medieval legal charters — The legend of “dux Ingo” and his “carta sine litteris”
No full textEnea Silvio Piccolomini in his work entitled
De Europa
written in 1458, tells an interesting story defined as a legend in terms of genre about a duke called Ingo, who lived during the reign of Charlemagne. This narrative claims that in 790
dux gentis
Ingo held a feast for the inhabitants of his province where food was served to the peasants allowed to appear before him in golden and silver bowls, while to the dignitaries standing further away from him in bowls made of clay. The researchers’ attention is deservedly raised by the query how come that this parabolical story with biblical tone was included in Enea Silvio’s work; if it had been borrowed who the
auctor
might have been he borrowed it from. The answer seems to be very simple: from the
Conversio Bagoariorum et Carantanorum
drafted regarding the lawsuit proceeded against Methodius. In the case narrated in the
Conversio
Ingo sent a charter or much rather a parchment without any writing, or letters on it
(carta sine litteris)
, which provided his legate with sufficient authenticity to demand obedience from the people.In this study-after having compared the two narratives and outlined the place of De Europa in Enea Silvio Piccolomini’s oeuvre and the circumstances of the drafting and tendencies of the Conversio Bagoariorum et Carantanorum-the author attempts to answer the following questions. To what extent can duke Ingo, mentioned by Enea Silvio and not questioned in the literature for long centuries, be considered a real historical person? Does the Conversio refer to Ingo as a duke, and if it does, what is his existence as a duke and introduction in the literature as a duke owing to? What could the meaning of carta sine litteris referred to in Conversio have been, and why did Enea Silvio not take this item over although he could have put it forward as a further proof of Ingo’s dignity? To what literary prefigurations can the description of the feast held by Ingo be traced back to, and what role did it play in the Conversio? Regarding the borrowing of the Ingo story by Enea Silvio, what possible intermediary writing and author can be reckoned with
Sammelrezension: Medien, Raum und Zeit
No full textJörg Döring, Tristan Thielmann (Hg.): Mediengeografie. <br />Theorie – Analyse – Diskussion <br />Ingo Köster, Kai Schubert (Hg.): Medien in Raum und Zeit. <br />Maßverhältnisse des Medialen<br /><br /
Endogenous sequence patterns predispose the repair modes of <scp>CRISPR</scp> /Cas9‐induced <scp>DNA</scp> double‐stranded breaks in <i>Arabidopsis thaliana</i>
No full textThe possibility to predict the outcome of targeted DNA double-stranded break (DSB) repair would be desirable for genome editing. Furthermore the consequences of mis-repair of potentially cell-lethal DSBs and the underlying pathways are not yet fully understood. Here we study the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-induced mutation spectra at three selected endogenous loci in Arabidopsis thaliana by deep sequencing of long amplicon libraries. Notably, we found sequence-dependent genomic features that affected the DNA repair outcome. Deletions of 1-bp to <1000-bp size and/or very short insertions, deletions >1 kbp (all due to NHEJ) and deletions combined with insertions between 5-bp to >100 bp [caused by a synthesis-dependent strand annealing (SDSA)-like mechanism] occurred most frequently at all three loci. The appearance of single-stranded annealing events depends on the presence and distance between repeats flanking the DSB. The frequency and size of insertions is increased if a sequence with high similarity to the target site was available in cis. Most deletions were linked to pre-existing microhomology. Deletion and/or insertion mutations were blunt-end ligated or via de novo generated microhomology. While most mutation types and, to some degree, their predictability are comparable with animal systems, the broad range of deletion mutations seems to be a peculiar feature of the plant A. thaliana
Correction: Discovering the opposite shore: How did hominins cross sea straits?
No full textThe ORCID iDs are missing for the second, fifth, and sixth authors. Please see the authors’ respective ORCID iDs here:
Author Christine Hertler’s ORCID iD is: 0000-0002-8252-9674 (https://orcid.org/0000-0002-8252-9674).
Author Jan Ole Berndt’s ORCID iD is: 0000-0001-7241-3291 (https://orcid.org/0000-0001-7241-3291).
Author Ingo J. Timm’s ORCID iD is: 0000-0002-3369-813X (https://orcid.org/0000-0002-3369-813X)
Authorship as cultural performance: new perspectives in authorship studies
No full textThis article proposes a performative model of authorship, based on the historical alternation between predominantly 'weak' and 'strong' author concepts and related practices of writing, publication and reading. Based on this model, we give a brief overview of the historical development of such author concepts in English literature from the Middle Ages to the twentieth century. We argue for a more holistic approach to authorship within a cultural topography, comprising social contexts, technological and media factors, and other cultural developments, such as the distinction between privacy and the public sphere
Assistance in medical insurance (on the example of InsCo "Ingo Ukraine")
No full textУ роботі розглядаються теоретичні аспекти розвитку медичного
страхування в Україні; особливостей врегулювання збитків у медичному страхуванні. Проаналізовано систему медичного ассистансу на прикладі ПраТ «Інго Україна»; проведена оцінка системи врегулювання збитків на підприємстві. Запропоновано напрями розвитку маркетингу у медичному страхуванні
України; шляхи вдосконалення системи врегулювання збитків.The work deals with the theoretical aspects development of medical
insurance in Ukraine; peculiarities of the settlement of losses in medical insurance. Author analysis a system of medical assistances with example on InsCo "Ingo Ukraine"; An estimation of the system of claims settlement at the company is carried out
- …
