1,354,857 research outputs found
Konsertot, klarinetti, ork., KV622, A-duuri
No full textSoitinnus: klarinetti, puhaltimia, jousiorkesteri.Sibelius-Akatemian konserttisarja 17.4.1982.Marja Haapamäki (klarinetti), jousiorkesteri, Jukka-Pekka Saraste (johtaja).Äänitetty: 17.4.1982, [Helsinki], [Sibelius-Akatemian] konserttisali (konserttitaltiointi).Digitoitu 9. 9. 2009
THE C-TERMINAL DOMAIN OF ALPHA-SPECTRIN IS STRUCTURALLY RELATED TO CALMODULIN
No full textAn alignment of amino acid sequences suggests that the spectrin domain, which contains two EF-hand calcium-binding motifs, is structurally related to calmodulin. It is possible to align approximately 160 residues at the C-terminus of alpha-spectrin with the entire calmodulin sequence. We have expressed this domain in Escherichia coli and purified it. Circular dichroic and nuclear magnetic resonance spectroscopy show that the protein is folded and mostly helical. The conformation of the protein, as monitored spectroscopically, is sensitive to calcium at 0.1-1.0 mM. Equilibrium dialysis shows that there are two binding sites within this domain, with affinities in the 0.5 mM range. The domain can be split into N-terminal and C-terminal halves which fold independently. Only the N-terminal subdomain binds calcium. These data suggest that the C-terminus of alpha-spectrin has a domain with a calmodulin fold and two calcium-binding sites. Sequence alignments suggest that the related domains in alpha-actinin, and possibly in dystrophin, may share the same calmodulin-like structure. However, only non-muscle alpha-actinins appear to have one or two EF-hand(s) with the calcium-binding consensus sequence, and a strict consensus is not found in the muscle alpha-actinins or dystrophins
Structural studies of the repeat motif of alpha-spectrin by NMR spectroscopy
No full textSpectrin, a major component of the membrane skeleton, is mainly composed of tandemly repeated segments of approx. 106 amino acids. We have undertaken the determination of the three-dimensional structure of a chicken brain alpha-spectrin repeat by heteronuclear multidimensional NMR. Sedimentation equilibrium demonstrates that this repeat is monomeric at the concentration used for NMR (1 mM). Its secondary structure was identified using a collection of sequential and medium range NOEs, chemical shifts, HN-Halpha coupling constants, and relaxation measurements. These data unequivocally demonstrate the presence of three long helices connected by two loops. A set of interhelical NOEs indicates that the helices assemble into a triple helical structure. Our results provide experimental evidence supporting the triple-helical bundle proposed by modelling
Early rectal cancer and screening for colorectal cancer
No full textColorectal cancer (CRC) is the second most common form of cancer in Europe, and population based screening for colorectal cancer is recommended by the European Union. Screening enables detection of precursor lesions, i.e. adenomas, and cancer at an early stage, and randomised trials have demonstrated that screening reduces mortality in colorectal cancer. In rectal cancer, oncological results after abdominal resection surgery have improved over many years, but the morbidity, mortality and negative functional side effects following surgery and oncological treatment are considerable. Local excision techniques, on the other hand, demonstrate excellent functional results and a low morbidity and mortality but have high local recurrence rates, mainly since the technique does not allow for excision of mesorectal lymph nodes, which could be exposed to metastatic disease not detectable in the preoperative radiological staging. Since further expansion of population based screening programs for CRC will increase the detection of early cancer, local excision techniques are of great interest, provided that an adequate oncological out-come can be ensured. In paper I all patients in Sweden undergoing surgery for stage I rectal cancer 1995-2006 were assessed regarding survival, local recurrence rates and risk factors for death. Patients undergoing local excision had a higher local recurrence rate and a poor survival, especially in the age group ≥ 80 years, compared to patients undergoing abdominal resection surgery. Paper II analysed risk factors for lymph node metastases in patients with rectal cancer. All patients in Sweden 2007-2010 with histopathologically confirmed radical resections of pT1-2 rectal cancer follow-ing abdominal resection surgery without (neo)adjuvant treatment were included. T2 stage, poor differen-tiation and vascular infiltration were identified as risk factors for lymph node metastases. A model calcu-lating the total risk depending on the number of risk factors included, displayed a risk range of 6-65 % and 11-78 % in T1 and T2 tumours respectively. In paper III all Swedish patients aged 60-69 years with screening detected colorectal cancer were com-pared to those with non-screening detected cancer diagnosed 2008-2012. Pre- and postoperative staging, MDT-assessment, surgical and oncological treatment were compared between the groups. Patients with screening detected cancer were staged and MDT-assessed to a higher extent compared to those with non-screening detected cancer and tumours were found at an earlier stage in the screening group. Surgical and oncological treatment did not differ between the groups. Patients with endoscopically resected can-cer did not undergo staging and MDT-assessment to the same extent as did patients with surgically re-sected cancer. Paper IV included all individuals with a positive FOBT in the Stockholm screening programme, January 2008 - June 2012. Complications and mortality within 30 days after interventions, i.e. colonoscopies or surgery for adenomas or cancer, subsequent to a positive screening test were assessed. Total complica-tion rates were acceptable and mortality was low, but the rate of anastomotic leakage, which was 13 % and 12 % in the adenoma and cancer surgery groups respectively, was higher than expected.List of scientific papersI. Local excision in early rectal cancer- outcome worse than expected: A population based study D. Saraste, U. Gunnarsson, M. Janson European Journal of Surgical Oncology 2013; 39: 634-639 https://doi.org/10.1016/j.ejso.2013.01.004 II. Predicting lymph node metastases in early rectal cancer D. Saraste, U. Gunnarsson, M. Janson European Journal of Cancer 2013; 49: 1104-1108 https://doi.org/10.1016/j.ejca.2012.10.005 III. Differences in pre-therapeutic work up and treatment in patients with screening vs. non-screening detected colorectal cancer D. Saraste, A. Martling, PJ. Nilsson, J. Blom, S. Törnberg, M. Janson [Submitted]IV. Complications and mortality after colonoscopy and surgery for colorec-tal cancer screening-detected adenomas and cancer D. Saraste, A. Martling, PJ. Nilsson, J. Blom, S. Törnberg, R. Hultcrantz. M Janson [Manuscript]</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Author, publisher and bookseller : a tripartite synergy in Nigerian book industry
No full textThis work is about the roles of Author, Publisher and Bookseller in Book development in
Nigeria. The paper started by delving into the history of Book Publishing in Nigeria after
which it proceeded by defining who an author, a publisher, and a bookseller is and
expatiated on the indispensable roles of these key actors in Nigerian Book Industry and in
the emerging Information Society. Furthermore, the various constraints to book
development were identified while the paper advised on how the Book Industry can be
further promoted in Nigeria. However, the paper concluded and made recommendations
on how the Book sector can help in enhancing scholarship in the country
The Thursday Murder Club: Launching a megabrand author - a publishing case study
No full textIn 2020, the Christmas book charts in the UK made headlines: Barack Obama’s eagerly awaited autobiography, The Promised Land, was beaten to the top spot by The Thursday Murder Club by Richard Osman, a debut cosy crime novel set in a retirement village. Not only did Osman’s book beat the former US president’s expected bestseller, it also broke records, becoming the fastest-selling debut crime novel of all time. Although Osman has a certain level of fame in the UK from his TV appearances on shows such as Pointless, his celebrity status does not entirely explain the novel’s huge sales. This article tracks the acquisition, publication, and promotion journey of The Thursday Murder Club in order to understand the industry and cultural context of its success and to interrogate the role of celebrity in the creation of author brands. The findings suggest that the unexpected scale of the success of the book owed to a number of factors, including in-depth editing by the novel’s agent, editor, and author to tighten up the plot, an extensive and strategic promotional campaign, the pandemic (which drove interest in the book’s genre and themes), and the quality of the writing. We find that the book’s success was accentuated by Osman’s celebrity status rather than being entirely reliant on it. This research adds to the growing scholarship on celebrity authorship by means of an in-depth case study and provides insight into the processes behind publishing a ‘celebrity’ book and launching a megabrand author
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