1,720,980 research outputs found
Konsentrasi Protein Antigen Ekskretori-Sekretori Fasciola Gigantica Asal Sapi Lokal Kabupaten Sigi, Sulawesi Tengah
No full textFasciolosis is a parasitic disease that caused by liver flukewormfromTrematode class and Fasciola genus with two important species, Fasciola hepatica and Fasciola gigantica (F.gigantica). Early diagnosis of fasciolosis can be detected by ELISA method to recognize antibody used antigen capture or known as coproantigen. Concentrations test on protein antigen (Ag) exretory-secretory (ES) of F. gigantica was conducted in September 2010. Antigen ES used in this test, is an AgES which had been produced in previous research. ES antigens can be used as a material for producing antibodies and as an ingredient in the ELISA test. The purpose of this studywas to determine the protein concentration of bovine AgESFg taken fromlocal cattle in Sigi district. The studywas an observational study with a cross-sectional study design. Samples of infected cattle liver were randomly taken from the Sigi Biromaru abattoir (slaughterhouse). ESFg antigens produced by collecting worms F. gigantica from Sigi local cattle and then isolated and incubated using RPMI culture medium for four hours at 370C. ESFg antigens characterized using Bradford method. Characterization produced AgES from three times collections of worms F. gigantica with obtained concentration were 0430, 0459, 0699 mg/ml within an average of 0.18 mg/worm. Therefore, it can be concluded that the protein concentration were 0,430- 0,699 mg/ml AgESFg taken from local cattle in Sigi district can be used as a material for producing antibodiesand asaningredient in the ELISA test.</p
ANTIBODI POLIKLONAL ANTI EKSKRETORI SEKRETORI (ES) Schistosoma japonicum PADA KAMBING (Capra aegagrus) BAHAN DETEKSI DINI PENDERITA SCHISTOSOMIASIS
No full textDi Indonesia Schistosomiasis masih merupakan masalah kesehatan masyarakat dengan prevalensi diatas 1%, dan untuk mendeteksi penderita menggunakan metode konvensional.Mendukung kegiatan deteksi penderita dilakukan penelitian produksi antibodi poliklonal anti ES S. japonicum pada kambing pada bulan Juni - Oktober 2012. Penelitian ini secara umum bertujuan untuk memproduksi antibodi poliklonal anti ES S. japonicum sebagai bahan dalam pendeteksian penderita schistosomiasis melalui uji ELISA.Metode yang digunakan untuk mendeteksi keberadaan antibodi poliklonal anti ES S. japonicum pada kambing adalah menggunakan uji Agar Gel Precipitation Test (AGPT) dan untuk mengukur konsentrasi menggunakan uji Bradford. Hasil dari penelitian ini yaitu antibodi poliklonal anti ES S. japonicum terbentuk pada minggu ke 12 pasca injeksi, dengan konsentrasi 0.931 mg/mL. Kesimpulan dari penelitian ini adalah waktu yang dibutuhkan dalam pembentukan antibodi untuk merespon antigen yang diinjeksi pada kambing yaitu selama 12 minggu. Konsentrasi antibodi poliklonal anti ES S. japonicum yang dihasilkan adalah cukup sebagai bahan dalam pengujian ELISA dan dapat mengenali antigen yang merangsang pembentukkan antibodi tersebut sehingga dapat digunakan dalam uji diagnostik kecacingan yang disebabkan oleh S. japonicum
TINGKAT KECACINGAN PADA ANAK SEKOLAH DASAR KECAMATAN LABUAN, KABUPATEN DONGGALA, SULAWESI TENGAH
No full textStool survey in three elementary schools had been conducted on Mei,2007, in Labuan,Dalika, and Toposo village, Labuan sub district, Donggala regency, Central Sulawesi province. The aim of this survey was to investigate the infection rate of helminthes with direct methoda. Results of this survey were 15,2 % of 33 children SDN Labuan was positive Ascaris lumricoides and 33,3 % of 21_children SDN Dalika so 14,3% of 12 children SDN Inpres 2 Toposo.One of them was positive Trichuris trichiura. Keywords : Ascaris lumricoides, Stool survey, Labuan.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
POTENSI KANDUNGAN KARONDO (Etlingera elatior) SEBAGAI OBAT CACING TRADISIONAL MASYARAKAT KULAWI DI SULAWESI TENGAH
No full textInfeksi cacingmerupakan masalah kesehatan yang termasuk dalam salah satu penyakit terabaikan (Negleted disease) dengan kejadian tertinggi di daerah pedesaan atau pelosok. Pengendalian oleh program kesehatan melalui pengobatan sangat terbatas kecuali bila terjadi kelainan atau keluhan berat dari pasien itu sendiri. Di Sulawesi Tengah Kabupaten Sigi khususnya masyarakat Kecamatan Kulawi menggunakan Etlingera elatior (karondo) sebagai obat tradisional untuk mengatasi kecacingan. Berdasarkan fakta yang ada maka dilakukan penelitian untuk mengetahui kandungan atau zat aktif sebagai anthelmintik dari tanaman karondo. Metode yang digunakan yaitu rimpang tanaman karondo diparut lalu dibuat dalam bentuk perasan murni kemudian air perasan murni diuji menggunakan uji kualitatif,dan hasilnya dilihat dengan adanya busa dan perubahan warna secara visual. Hasil dari uji yang dilakukan yaitu adanya kandungan tannin, terpenoid, flavonoid, fenolat dan saponin dalam rimpang tanaman karondo.Disimpulkan bahwa tanaman Etlingera elatior (karondo) atau dalam bahasa Indonesia kecombrang mempunyai potensi sebagai bahan baku tanaman obat untuk mengatasi infeksi cacing di masyarakat
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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