1,355,108 research outputs found
Early growth response-1 is a regulator of DR5-induced apoptosis in colon cancer cells
No full textBACKGROUND: Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) induces tumour cell apoptosis by binding to death receptor 4 (DR4) and DR5. DR4 and DR5 activation however can also induce inflammatory and pro-survival signalling. It is not known how these different cellular responses are regulated and what the individual role of DR4 vs DR5 is in these processes.METHODS: DNA microarray study was carried out to identify genes differentially expressed after DR4 and DR5 activation. RT-PCR and western blotting was used to examine the expression of early growth response gene-1 (Egr-1) and the proteins of the TRAIL signalling pathway. The function of Egr-1 was studied by siRNA-mediated knockdown and overexpression of a dominant-negative version of Egr-1.RESULTS: We show that the immediate early gene, Egr-1, regulates TRAIL sensitivity. Egr-1 is constitutively expressed in colon cancer cells and further induced upon activation of DR4 or DR5. Our results also show that DR4 mediates a type II, mitochondrion-dependent apoptotic pathway, whereas DR5 induces a mitochondrion-independent, type I apoptosis in HCT15 colon carcinoma cells. Egr-1 drives c-FLIP expression and the short splice variant of c-FLIP (c-FLIPS) specifically inhibits DR5 activation.CONCLUSION: Selective knockdown of c-FLIPS sensitises cells to DR5-induced but not DR4-induced apoptosis and Egr-1 exerts an effect as an inhibitor of the DR5-induced apoptotic pathway, possibly by regulating the expression of c-FLIPS. British Journal of Cancer (2010) 102, 754-764. doi:10.1038/sj.bjc.6605545 www.bjcancer.com Published online 19 January 2010 (C) 2010 Cancer Research U
Assessment of key response quantities for design of a cable-stayed bridge subjected to sudden loss of cable(s)
No full textStays of cable-stayed bridges have potential to lose their support instantly by extreme loadings such as earthquake, thunder strike, vehicle impact, wind and malicious attacks. The sudden loss of cable(s) provides unpredictable stress redistribution on the deck and towers, as well as the large deflections of the entire bridge. This phenomenon is the cause of one of the most critical situations for the cable-stayed bridges, termed "zipper-type collapse" (i.e. the mechanism of the progressive collapse by the redistribution of stresses). Considering such a sudden loss of cable in the design of a cable-stayed bridge is essential. Although cable loss scenarios are associated with material as well as geometrical nonlinearities, in design of cable-stayed bridges, such an extreme loading scenario is analysed typically by using linear elastic models. In this paper, a linear elastic 2D and a fully nonlinear 3D finite element model of an idealised steel cable-stayed bridge are developed and analysed to determine the effect of sudden loss of cable on the progressive collapse of the bridge at global and local stress levels.A parametric dynamic analysis for the bridge model with different cable loss scenarios under symmetrical or unsymmetrical load cases is investigated. The bridge model studied in this paper showed that the 2D model can adequately capture global behaviour of the bridge. The 3D model showed the most significant stress concentration occurring under the symmetrical case are limited to small areas and are negligible. © 2013 Taylor & Francis Group
505 Dual Cdc7/Cdk9 kinase inhibitor, PHA-767491, targets both quiescent and proliferating CLL cells
No full textPerformance of an active mass driver system on a five storey benchmark model
No full textThis paper reports the experimental tests conducted on a 5-storey benchmark model defined by Samali, using an Active Mass Driver (AMD) system, where the control action is achieved by using Fuzzy Logic controller and UTS state-of-the-art shake table facility. The performance of the Fuzzy controller is checked against Hachinohe 1968 and Northridge 1994 earthquake records as input excitation to the benchmark model. The main advantage of the Fuzzy controller is its inherent robustness and ability to handle any non-linear behaviour of the structure. The results of the experimental tests show the ability of the adopted Fuzzy controller to reduce the building responses for the two earthquake records used
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Spatial distribution, feeding and length-mass relationships of Diamphipnopsis samali (Plecoptera, Diamphipnoidae) in a North Patagonia Andean stream, Argentina
No full textRESUMEN. Fueron estudiados algunos aspectos de la biología y ecología (patrones de distribución espacial, hábitos alimenticios y relación largo-masa) de una población de Diamphipnopsis samali en un arroyo de primer orden que atraviesa un bosque exótico de pino en el centro-norte de Patagonia (Chubut, Argentina). Dos series de muestreos fueron realizados durante períodos de aguas altas y aguas bajas. Toda la información fue tratada con ANOVAS de dos factores y efectos fijos para evaluar diferencias significativas. El análisis de las piezas bucales de D. samali indica que corresponden a un tipo intermedio entre el tipo carnívoro y herbívoro-detritívoro. El contenido estomacal muestra que esta especie se alimenta de hifas fúngicas y madera, aunque en los estadios tempranos se encuentran restos de insectos. No se hallaron diferencias significativas en la alimentación entre períodos de aguas altas y aguas bajas, sin embargo, existieron diferencias significativas entre la dieta de larvas grandes y pequeñas. Fue clara la preferencia por el sustrato tronco y cascada, y también significativamente diferente entre los tipos pozón y corredera. No hubo diferencias significativas en la elección de hábitat entre los períodos de aguas bajas y aguas altas. Esta especie endémica de Patagonia parece estar bien adaptada a la presencia de un bosque ripario exótico, utilizando los troncos caídos como alimento y refugio
Evaluación económica y financiera privada e individual del proyecto Casa Samali en el sector gastronómico de Granada, Santiago de Cali
No full textTesis de EconomíaEl objetivo principal del presente trabajo es determinar la viabilidad económica y financiera del proyecto productivo ¿CASA SAMALI¿. Lo anterior, se determinará a través del enfoque de flujo de caja descontado planteando el plan de negocio con base a la metodología del fondo emprender, en conjunto con un análisis del sector hoteles, restaurantes y turismo en el cual el proyecto está catalogado
Dynamic behaviour of flexible facade systems in tall buildings subjected to wind loads
No full textIn recent years many new devices, as energy absorber systems, have been introduced in tall building. But most of them have some disadvantages such as complexity of design and large spaces needed for their installation. Façade systems, as an integral part of high-rise buildings, and as non-structural systems are susceptible to potential failure when subjected to wind, if not designed properly. In this paper an alternative method using multi-skin façade systems as an energy absorbing device is proposed in order to decrease the amount of energy imparted to the main structure during high speed winds and cyclones. A series of elastic and inelastic time history analyses using SAP2000 have been carried out to assess the structural dynamic response of a proposed 45 storey building with reinforced concrete frame structure under a simulated harmonic wind load, which derived from three second gusts according to provisions of Australian wind code (AS/NZ 1170.2). The preliminary results indicate that it is possible to make use of the double skin façade system in tall buildings to work as a damper device, if the connections are properly designed, to minimise the wind induced vibrations of the primary structure as well as reducing inter storey drift. © 2013 Taylor & Francis Group
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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