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Intra-specific biodiversity of Italian myrtle (Myrtus communis)through chemical markers profile and biological activities of leafmethanolic extracts
No full textIn vitro investigation of the potential health benefit of wild mediterranean dietary plants as antiobesity with alpha-amilase and pancreatic lipase inhibitory action
No full textFig latex (Ficus carica L. cultivar Dottato) in combination with UV irradiation decreases the viability of A375 melanoma cells in vitro
No full textMelanoma and nonmelanoma skin cancers are among the most prevalent cancers in the human population. In the present work latex of Ficus carica cultivar Dottato from Italy collected from fruits and leaves was examined to assess its free radical-scavenging activity with 1,1-diphenyl-2 picrylhydrazyl (DPPH) and its phototoxicity on A375 human melanoma cells. The latex obtained from the fruits of Ficus carica cv. Dottato showed the best antiradical activity with an IC50 value of 0.05 mg/ml while the latex obtained from the leaves showed the best antiproliferative activity with an IC50 value of 1.5 μg/ml on the human tumor cell line A375 (melanoma) after irradiation at a specific UVA dose (1.08 J/cm2). Control experiments with UVA light or drugs alone were carried out without significant cytotoxic effects. Polyphenolic content of the samples was also evaluated. This is the first study comparing F. carica latex of leaves and fruits. Plant derived natural products have long been and will continue to be an important source for anticancer drug development
Fig latex (Ficus carica L. cultivar Dottato) in combination with UV irradiation decreases the viability of A375 melanoma cells in vitro.
No full textMelanoma and nonmelanoma skin cancers are among the most prevalent cancers in the human population. In the present work latex of Ficus carica cultivar Dottato from Italy collected from fruits and leaves was examined to assess its free radical-scavenging activity with 1,1-diphenyl-2 picrylhydrazyl (DPPH) and its phototoxicity on A375 human melanoma cells. The latex obtained from the fruits of Ficus carica cv. Dottato showed the best antiradical activity with an IC50 value of 0.05 mg/ml while the latex obtained from the leaves showed the best antiproliferative activity with an IC50 value of 1.5 μg/ml on the human tumor cell line A375 (melanoma) after irradiation at a specific UVA dose (1.08 J/cm2). Control experiments with UVA light or drugs alone were carried out without significant cytotoxic effects. Polyphenolic content of the samples was also evaluated. This is the first study comparing F. carica latex of leaves and fruits. Plant derived natural products have long been and will continue to be an important source for anticancer drug development
"Cachrys pungens Jan inhibits human melanoma cell proliferation through photo-induced cytotoxic activity."
No full textObjective: To date, plants belonging to the genus Cachrys have not been amply studied. In the present study, aerial components of Cachrys pungens Jan from Italy, were examined to assess their free radical-scavenging and antioxidant activity, and their phototoxicity on A375 melanoma cells. In view of potential pharmaceutical applications, a relationship between antioxidant, phototoxic activities and polyphenolic composition has also been investigated. Materials and methods: Content of sterols, terpenes, fatty acids and coumarins was assessed by gas chromatography-mass spectrometry and GC. Total phenolic content was also determined. Antioxidant activity of the methanol extract and fractions of C. pungens Jan was assessed using DPPH scavenging assay and β-carotene bleaching test. Plant phototoxicity was also investigated in this human tumour cell line (amelanotic melanoma). Results: Analysis of the chloroform extract was particularly interesting, as it led to identification of many coumarins, of which five were linear and one angular furanocoumarins. Methanol and ethyl acetate fractions exhibited substantial antioxidant activity. Moreover, chloroform extract and isolated coumarin fraction had strong phototoxic activity on UVA-induced A375 cells after irradiation at UVA dose of 1.08J/cm. Conclusions: Plant-derived natural compounds are an important source for development of cancer-fighting drugs. This study has demonstrated strong phototoxic activity of the coumarin fraction of C. pungens, a plant which, to our knowledge, has never been studied before. This investigation offers a new perspective for developing other formulations potentially useful in photodynamic therapy for treatment of non-melanoma skin cancers as well as melanomas
Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma
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Cell Proliferation
Volume 45, Issue 3, June 2012, Pages 279-285
Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma (Article)
Conforti, F.a ,
Menichini, G.b,
Zanfini, L.a,
Tundis, R.a,
Statti, G.A.a,
Provenzano, E.c,
Menichini, F.a,
Somma, F.b,
Alfano, C.b
a Department of Pharmaceutical Sciences, Faculty of Pharmacy Nutrition and Health Sciences, University of Calabria, Rende (CS), Italy
b Department of Plastic and Reconstructive Surgery, Perugia University, Perugia, Italy
c Operative Unit of Dermatology, A.O. of Cosenza, Cosenza, Italy
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Abstract
Objectives: To date, Ficus carica L. cultivar Dottato (F. carica) has not been studied from a phototoxic point of view. In the present work, aerial components of F. carica from Italy, were examined to assess their antioxidant and phototoxic activity on human melanoma cells. A relationship between antioxidant, phototoxic activities and chemical composition has also been investigated. Materials and methods: Coumarin and fatty acid content in F. carica leaves, bark and woody parts were examined and compared by capillary GC and GC/MS. Polyphenolic content was also determined. Linoleic acid peroxidation and DPPH test were used to assess antioxidant activities, and MTT assay was used to evaluate anti-proliferative activity, on C32 human melanoma cells, after irradiation with a UVA dose of 1.08 J/cm 2. Results: Leaves demonstrated the best antioxidant and anti-proliferative activity in comparison to bark and wood. In particular, leaves were shown to possess the highest anti-radical activity and inhibition of peroxidation, with IC 50 values of 64 and 1.48 μg/ml respectively. The leaves had highest anti-proliferative activity with IC 50 value of 3.92 μg/ml. The phytochemical investigation revealed different composition between the coumarins, psoralen and bergapten, fatty acids, polyphenols and flavonoid content among plant parts. Conclusions: Data obtained indicate that this type of fig tree may constitute an excellent source of bioactive compounds, such as phenolics, coumarins and fatty acids. This study offers a new perspective in developing others formulations potentially useful in photodynamic therapy for treatment of non-melanoma skin cancer
Phytochemical analysis and in vitro evaluation of the biological activity against herpes simplex virus type 1 (HSV-1) of Cedrus libani A. Rich
No full textCedrus libani are widely used as traditional medicine in Lebanon for treatment of different infection diseases. In the present study we reported the phytochemical composition analyzed by GC-MS of wood essential oil and cones and leaves ethanol extracts. The main components of wood essential oil were himachalol (22.50%), beta-himachalene (21.90%), and alpha-himachalene (10.50%). Leaves ethanol extract was characterized by a high content of germacrene d (29.40%). The same extract obtained from cones essentially contained alpha-pinene (51.0%) and beta-myrcene (13.0%). Moreover, we investigated extracts, essential oil, and identified compounds for their in vitro antiviral activities against herpes simplex virus type 1 (HSV-1). Cytotoxicity was evaluated by MTT assay in Vero cells. Cones and leaves ethanol extracts exhibited an interesting activity with IC(50) of 0.50 and 0.66mg/ml, respectively, at non-cytotoxic concentration. A comparable activity was found when essential oil was tested (IC(50) of 0.44mg/ml)
Antioxidant, enzyme-inhibitory and antitumor activity of the alimurgic dietary plant Muscari comosum (L.) Mill.
Full text linkThe pharmaceutical properties of the alimurgic species Muscari comosum are mostly unknown. In this experiment, total extracts from bulbs of M. comosum were tested for a) free radical scavenging activity, b) enzymatic inhibition of α-amylase and lipase, and c) inhibition of the growth of breast adenocarcinoma (MCF-7) cells. Three treatments were considered: bulbs boiled for 15 min (BB), bulbs steam-cooked for 15 min (SB), and raw bulbs (RB). The polyphenol content and antioxidant capacity of the extracts were related to the inhibition of pancreatic lipase and α-amylase, whose activities showed a half maximal inhibitory concentration (IC50) of 0.28, 2.14 and 3.22 mg/mL for lipase, and 0.16, 0.73 and 0.69 mg/mL for α-amylase in RB, SB and BB, respectively. The proliferation of MCF-7 cells was inhibited by RB and BB extracts. M. comosum could be an excellent plant for the development of anti-obesity drugs, also able to prevent cancer
Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species
No full textThe chemical composition of the essential oils of Laurus nobilis, Juniperus oxycedrus ssp. oxycedrus, Thuja orientalis, Cupressus sempervirens ssp. pyramidalis, Pistacia palaestina, Salvia officinalis, and Satureja thymbra was determined by GC/MS analysis. Essential oils have been evaluated for their inhibitory activity against SARS-CoV and HSV-1 replication in vitro by visually scoring of the virus-induced cytopathogenic effect post-infection. L. nobilis oil exerted an interesting activity against SARS-CoV with an IC(50) value of 120 microg/ml and a selectivity index (SI) of 4.16. This oil was characterized by the presence of beta-ocimene, 1,8-cineole, alpha-pinene, and beta-pinene as the main constituents. J. oxycedrus ssp. oxycedrus oil, in which alpha-pinene and beta-myrcene were the major constituents, revealed antiviral activity against HSV-1 with an IC(50) value of 200 microg/ml and a SI of 5
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