2,984 research outputs found
Functionalized nanostructured chitosan-based devices for peripheral nerve regeneration
No full textPeripheral nerve injuries (PNIs) are a common global issue lacking definitive treatments. Regenerative medicine introduces promising methods to bridge nerve gaps, with a strong focus on biocompatible, biodegradable, and mechanically compliant materials. Chitosan, an FDA-approved biopolymer, stands out for its biocompatibility and biodegradability. Its regenerative potential improves with nano/micro-structuring, which guides cells through biomimetic topographical signals, and blending with other materials enhances its mechanical compatibility with host tissues.This thesis focuses on developing chitosan-based scaffolds for peripheral nerve regeneration. A method to create micro-structured chitosan films with reproducible patterns was designed, producing three directional micro-grooved patterns: gratings (GR), isosceles triangles (ISO), and scalene triangles (SCA). These structured films were tested in vitro with Schwann cells, showing variations in cell migration across the patterns.To address chitosan’s rigidity, soft chitosan membranes were created using glycerol as a bioplasticizer, reducing stiffness and enhancing suitability for clinical use. My work revealed that membrane flexibility and surface structures influence neural cell responses, with softer, micro-structured membranes leading to improved cell behavior compared to stiffer versions.Further evaluations employed an in vitro nerve model with primary Schwann cells and nociceptive neurons, developed in collaboration with the MERLN Institute (University of Maastricht) to study the effects of micro-structured glycerol-chitosan membranes. Additionally, these membranes were tested ex vivo with dorsal root ganglia (DRGs) from rats and in vivo in a cavernous nerve injury model in collaboration with the University of Turin.Finally, to develop even smaller features, glycerol-plasticized chitosan nanofibers were developed and characterized, demonstrating promising potential for use in nerve regeneration applications.In summary, this thesis highlights chitosan’s potential for peripheral nerve repair, showcasing its micro and nanostructurability, biocompatibility, and suitability in supporting nerve regeneration. This research demonstrates chitosan’s capacity to advance regenerative medicine, offering valuable biomaterials for peripheral nerve injury treatment
An assessment of the impact of possible CAP reform scenarios on Romanian agriculture
No full textUsing a simplified model, with key-variable the prices of two different possible scenarios of CAP reform after 2013 (moderate and radical), this paper present a comparison between the price effects of implementation of each reform scenario at 2015 horizon on Romanian agriculture. This short analysis shows that, under the presented hypotheses, the net welfare effect, due to the price changes, for the selected products, is positive in both reform scenarios, yet greater in the case of the radical reform. Integrated in the large context of Romanian development, it seems that the influence of CAP reform upon agriculture and rural areas will be most likely a gradual one: an interpenetration between the two scenarios is foreseeable, starting with the moderate reform that will dominate the period around 2013, the reform measures acquiring a more radical character afterwards.CAP reform, Romania, welfare effects, Agricultural and Food Policy,
Rich, Sturmian, and trapezoidal words
No full textIn this paper we explore various interconnections between rich words, Sturmian words, and trapezoidal words. Rich words, first introduced by the second and third authors together with J. Justin and S. Widmer, constitute a new class of finite and infinite words characterized by having the maximal number of palindromic factors. Every finite Sturmian word is rich, but not conversely. Trapezoidal words were first introduced by the first author in studying the behavior of the subword complexity of finite Sturmian words. Unfortunately this property does not characterize finite Sturmian words. In this note we show that the only trapezoidal palindromes are Sturmian. More generally we show that Sturmian palindromes can be characterized either in terms of their subword complexity (the trapezoidal property) or in terms of their palindromic complexity. We also obtain a similar characterization of rich palindromes in terms of a relation between palindromic complexity and subword complexity
Distribution of the genus Platycerus Geoffroy (Coleoptera, Lucanidae) in Italy
No full textStag beetles are saproxylic species that are associated with deadwood in the larval stage and play an important role in forest ecosystem dynamics. In Italy, Platycerus caprea and Platycerus caraboides are two small, elusive stag beetle species, whose known distribution is often limited or referring to old records.The present contribution increases the knowledge on the Italian distribution of P. caprea and P. caraboides, adding 70% more sites when compared to previously published records. Ecological traits, especially related to the altitude-elevation distribution in Italy, are also described for these saproxylic beetles
Glycerol-blended chitosan membranes with directional micro-grooves and reduced stiffness improve Schwann cell wound healing
No full textRegenerative medicine is continuously looking for new natural, biocompatible and possibly biodegradable materials, but also mechanically compliant. Chitosan is emerging as a promising FDA-approved biopolymer for tissue engineering, however, its exploitation in regenerative devices is limited by its brittleness and can be further improved, for example by blending it with other materials or by tuning its superficial microstructure. Here, we developed membranes made of chitosan (Chi) and glycerol, by solvent casting, and micro-patterned them with directional geometries having different levels of axial symmetry. These membranes were characterized by light microscopies, atomic force microscopy (AFM), by thermal, mechanical and degradation assays, and also tested in vitro as scaffolds with Schwann cells (SCs). The glycerol-blended Chi membranes are optimized in terms of mechanical properties, and present a physiological-grade Young's modulus (approximate to 0.7 MPa). The directional topographies are effective in directing cell polarization and migration and in particular are highly performant substrates for collective cell migration. Here, we demonstrate that a combination of a soft compliant biomaterial and a topographical micropatterning can improve the integration of these scaffolds with SCs, a fundamental step in the peripheral nerve regeneration process
Chitosan Micro-Grooved Membranes with Increased Asymmetry for the Improvement of the Schwann Cell Response in Nerve Regeneration
No full textPeripheral nerve injuries are a common condition in which a nerve is damaged, affecting more than one million people every year. There are still no efficient therapeutic treatments for these injuries. Artificial scaffolds can offer new opportunities for nerve regeneration applications; in this framework, chitosan is emerging as a promising biomaterial. Here, we set up a simple and effective method for the production of micro-structured chitosan films by solvent casting, with high fidelity in the micro-pattern reproducibility. Three types of chitosan directional micro-grooved patterns, presenting different levels of symmetricity, were developed for application in nerve regenerative medicine: gratings (GR), isosceles triangles (ISO) and scalene triangles (SCA). The directional patterns were tested with a Schwann cell line. The most asymmetric topography (SCA), although it polarized the cell shaping less efficiently, promoted higher cell proliferation and a faster cell migration, both individually and collectively, with a higher directional persistence of motion. Overall, the use of micro-structured asymmetrical directional topographies may be exploited to enhance the nerve regeneration process mediated by chitosan scaffolds
Characterization Results for the Poset Based Representation of Topological Relations - I: Introduction and Models
No full text@article{DBLP:journals/informaticaSI/ForlizziN99,
author = {Luca Forlizzi and
Enrico Nardelli},
title = {Characterization Results for the Poset Based Representation
of Topological Relations - I: Introduction and Models.},
journal = {Informatica (Slovenia)},
volume = {23},
number = {2},
year = {1999},
bibsource = {DBLP, http://dblp.uni-trier.de}
Characterization Results for the Poset Based Representation of Topological Relations - II: Intersection and Union
No full text@article{DBLP:journals/informaticaSI/ForlizziN00,
author = {Luca Forlizzi and
Enrico Nardelli},
title = {Characterization Results for the Poset Based Representation
of Topological Relations - II: Intersection and Union.},
journal = {Informatica (Slovenia)},
volume = {24},
number = {1},
year = {2000},
bibsource = {DBLP, http://dblp.uni-trier.de}
System-on-chip Computing and Interconnection Architectures for Telecommunications and Signal Processing
No full textThis dissertation proposes novel architectures and design techniques targeting SoC building blocks for telecommunications and signal processing applications.
Hardware implementation of Low-Density Parity-Check decoders is approached at both the algorithmic and the architecture level. Low-Density Parity-Check codes are a promising coding scheme for future communication standards due to their outstanding error correction performance.
This work proposes a methodology for analyzing effects of finite precision arithmetic on error correction performance and hardware complexity. The methodology is throughout employed for co-designing the decoder. First, a low-complexity check node based on the P-output decoding principle is designed and characterized on a CMOS standard-cells library. Results demonstrate implementation loss below 0.2 dB down to BER of 10^{-8} and a saving in complexity up to 59% with respect to other works in recent literature. High-throughput and low-latency issues are addressed with modified single-phase decoding schedules. A new "memory-aware" schedule is proposed requiring down to 20% of memory with respect to the traditional two-phase flooding decoding. Additionally, throughput is doubled and logic complexity reduced of 12%. These advantages are traded-off with error correction performance, thus making the solution attractive only for long codes, as those adopted in the DVB-S2 standard. The "layered decoding" principle is extended to those codes not specifically conceived for this technique. Proposed architectures exhibit complexity savings in the order of 40% for both area and power consumption figures, while implementation loss is smaller than 0.05 dB.
Most modern communication standards employ Orthogonal Frequency Division Multiplexing as part of their physical layer. The core of OFDM is the Fast Fourier Transform and its inverse in charge of symbols (de)modulation. Requirements on throughput and energy efficiency call for FFT hardware implementation, while ubiquity of FFT suggests the design of parametric, re-configurable and re-usable IP hardware macrocells. In this context, this thesis describes an FFT/IFFT core compiler particularly suited for implementation of OFDM communication systems. The tool employs an accuracy-driven configuration engine which automatically profiles the internal arithmetic and generates a core with minimum operands bit-width and thus minimum circuit complexity. The engine performs a closed-loop optimization over three different internal arithmetic models (fixed-point, block floating-point and convergent block floating-point) using the numerical accuracy budget given by the user as a reference point. The flexibility and re-usability of the proposed macrocell are illustrated through several case studies which encompass all current state-of-the-art OFDM communications standards (WLAN, WMAN, xDSL, DVB-T/H, DAB and UWB). Implementations results are presented for two deep sub-micron standard-cells libraries (65 and 90 nm) and commercially available FPGA devices. Compared with other FFT core compilers, the proposed environment produces macrocells with lower circuit complexity and same system level performance (throughput, transform size and numerical accuracy).
The final part of this dissertation focuses on the Network-on-Chip design paradigm whose goal is building scalable communication infrastructures connecting hundreds of core. A low-complexity link architecture for mesochronous on-chip communication is discussed. The link enables skew constraint looseness in the clock tree synthesis, frequency speed-up, power consumption reduction and faster back-end turnarounds. The proposed architecture reaches a maximum clock frequency of 1 GHz on 65 nm low-leakage CMOS standard-cells library. In a complex test case with a full-blown NoC infrastructure, the link overhead is only 3% of chip area and 0.5% of leakage power consumption.
Finally, a new methodology, named metacoding, is proposed. Metacoding generates correct-by-construction technology independent RTL codebases for NoC building blocks. The RTL coding phase is abstracted and modeled with an Object Oriented framework, integrated within a commercial tool for IP packaging (Synopsys CoreTools suite). Compared with traditional coding styles based on pre-processor directives, metacoding produces 65% smaller codebases and reduces the configurations to verify up to three orders of magnitude
Genetic variability of Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) and its symbiotic bacteria ‘Candidatus Pantoea carbekii’ across native and newly colonized regions.
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