2,600 research outputs found
In situ amplification of jaagsiekte retrovirus in Sardinian moufflons affected by pulmonary adenomatosis
No full textAMPLIFICAZIONE E SEQUENZIAMENTO DELLA REGIONE U3-LTR DI JAAGSIEKTE SHEEP RETRO-VIRUS (JSRV) DA OVIS ARIES E OVIS MUSIMON NATURALMENTE AFFETTI DA ADENOMATOSI POLMONARE
No full textAn elderly female patient with tardive oromandibular dystonia after prolonged use of the histamine analog betahistine.
No full textHelicobacter pylori infection in shepherds, sheep and sheep-dogs
No full textObjectives: Previous studies demonstrated a high prevalence of Helicobacter pylori infection
among shepherds, sheep, sheep raw milk and children having contact with sheep, suggesting a model of transmission
of infection at elevated frequency. The aim of this study was to examine whether H. pylori infection might
be present in sheep-dogs.
Material and Methods: Stool, serum, and wool were collected from sheep and sheep-dogs belonging to different
flocks chosen because the shepherds had documented (13C-UBT) H. pylori infection. H. pylori status in
the animals was evaluated using a homemade anti-H. pylori IgG enzyme-linked immunosorbent assay (ELISA)
for sheep and sheep-dogs. Stool samples from sheep and sheep-dogs were also analyzed using the stool antigen
test (HpSA) developed for humans. Results were expressed as optical density (OD450). PCR amplification was
performed using primers specific for H. pylori [cagA, vacA (s and m region), iceA, babA2] as well as primers
specific for Helicobacter species (16S rRNA) from the DNA extracted from sheep wool wash surrounding the
mammalian glands.
Results: 58 animals were studied (44 sheep, 8 lambs and 6 sheep-dogs). The H. pylori antigen test was positive in
82% (36/44) and in 100% of sheep and sheep-dogs stool samples, respectively. Lamb stool samples were weakly
positive. High anti H. pylori IgG serum levels were detected in all 6 sheep-dogs and in 42 of 44 sheep. Genomic
H. pylori and Helicobacter species DNA was not detected from any of the sheep wool samples collected.
Conclusions: Although cross-reactivity cannot be excluded, the presence of the H. pylori antigen in stools obtained
from sheep and from sheep-dogs, and the strong positivity for anti H. pylori IgG in both coupled with the
ability to isolate H. pylori from sheep milk and stomachs, suggest that interaction between species may enhance
the spread of bacteria in heavily contaminated settings
Diazepam enhances bicuculline-induced increase of t-[35S]butylbicyclophosphorothionate binding in unwashed membrane preparations from rat cerebral cortex.
No full textThe effect of diazepam on t-[35S]butylbicyclophosphorothionate ([35S]TBPS) binding to membrane preparations from rat cerebral cortex was examined in the presence or absence of GABA. The in vitro addition of diazepam to unwashed membranes preparations (rich in endogenous GABA) decreased [35S]TBPS binding by 41%. On the contrary, diazepam produced an opposite effect (+28%) when GABA had been removed by extensive washes of membranes. Moreover, diazepam increased by 26% [35S]TBPS binding also in unwashed membrane preparations previously incubated with bicuculline. These results suggest that, in absence of gamma-aminobutyric acid (GABA), diazepam may have a paradoxical negative modulatory action on the function of the GABAA receptor-coupled chloride channels
A technical refinement to prevent supratip deformity in aesthetic rhinoplasty: "the trapezoid peak"
No full textSynthesis, structure-activity relationships at the GABA(A) receptor in rat brain, and differential electrophysiological profile at the recombinant human GABA(A) receptor of a series of substituted 1,2-diphenylimidazoles
No full textA series of new 1,2-diphenylimidazole derivatives (la-x) were synthesized and evaluated for their ability to potentiate gamma-aminobutyric acid (GABA)-evoked currents in Xenopus laevis oocytes expressing recombinant human GABAA receptors. Many of these compounds enhanced GABA action with potencies (EC50 = 0.19-19,mu M) and efficacies (maximal efficacies of up to 640%) similar to or greater than those of anesthetics such as etomidate, propofol, and alphaxalone. Structure- activity relationship analysis revealed that the presence of an ester moiety in the imidazole ring was required for full agonist properties, while modifications made in the phenyl rings affected potency and efficacy, with ethyl 2-(4-bromophenyl)-l-(2,4-dichlorophenyl)-1H4-imidazolecarboxylate showing the highest potency. These compounds potentiated the [H-3]GABA binding to rat brain membranes, suggesting a site of interaction different from that of GABA. As for etomidate, mutation of asparagine-265 in the beta 2 subunit of the GABA(A) receptor into serine reduced the ability of derivative 1i to modulate the GABA function
Association of jaagsiekte sheep retrovirus with pulmonary carcinoma in sardinian moufflon (Ovis musimon)
No full textBronchiolo-alveolar carcinoma has been described in man and in several animal species, including cattle, dogs, opossums, goats and sheep. In sheep, a bronchiolo-alveolar carcinoma, known as ovine pulmonary carcinoma (OPC), is caused by jaagsiekte sheep retrovirus JSRN, an exogenous type D retrovirus. In the mid-1980s, a severe outbreak of a disease resembling OPC was described in captive Sardinian moufflon (Ovis musimon). In the present study, the use of polymerase chain reaction (PCR) amplification of nucleic acids extracted from archival material established that JSRV was associated with OPC in affected moufflon. JSRV was detected in the lungs and mediastinal lymph nodes. Immunohistochemical and in-situ PCR demonstrated that in the lungs, JSRV proviral. DNA was localized in transformed and untransformed type II pneumocytes and in the alveolar macrophages. In the mediastinal lymph nodes, JSRV DNA was mainly located in the cortical follicles and paracortex. These data suggest that JSRV is the cause of OPC in Sardinian moufflon, as it is in Sardinian sheep. (C) 2001 Harcourt Publishers Ltd
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