72,307 research outputs found
Post-translational formylglycine modification of bacterial sulfatases by the radical S-adenosylmethionine protein AtsB
No full textFang QH, Peng JH, Dierks T. Post-translational formylglycine modification of bacterial sulfatases by the radical S-adenosylmethionine protein AtsB. JOURNAL OF BIOLOGICAL CHEMISTRY. 2004;279(15):14570-14578.C-alpha-Formylglycine (FGly) is the catalytic residue of sulfatases. FGly is generated by post-translational modification of a cysteine ( prokaryotes and eukaryotes) or serine ( prokaryotes) located in a conserved (C/S) XPXR motif. AtsB of Klebsiella pneumoniae is directly involved in FGly generation from serine. AtsB is predicted to belong to the newly discovered radical S-adenosylmethionine (SAM) superfamily. By in vivo and in vitro studies we show that SAM is the critical co-factor for formation of a functional AtsB . SAM . sulfatase complex and for FGly formation by AtsB. The SAM-binding site of AtsB involves (83)GGE(85) and possibly also a juxtaposed FeS center coordinated by Cys(39) and Cys(42), as indicated by alanine scanning mutagenesis. Mutation of these and other conserved cysteines as well as treatment with metal chelators fully impaired FGly formation, indicating that all three predicted FeS centers are crucial for AtsB function. It is concluded that AtsB oxidizes serine to FGly by a radical mechanism that is initiated through reductive cleavage of SAM, thereby generating the highly oxidizing deoxyadenosyl radical, which abstracts a hydrogen from the serine-CbetaH2-OH side chain
Fang Fang
No full textBiochemistry & Molecular BiologyCell BiologySCI(E)0EDITORIAL MATERIAL12R444-R4462
Does Downloading PowerPoint Slides Before the Lecture Lead to Better Student Achievement?: Reply
Get PDFThis reply responds to a comment by Cannon (2011) that opens the debate on consistency of the effect of downloading PowerPoint slides before lectures on students’ exam performance. Cannon (2011) points out potential endogeneity problems in Chen and Lin (2008) and attempts to explore the unconditional mean effect of downloading PowerPoint slides for the full sample. In this reply, we firstly argue that the estimates in our original article are consistent since the effect of interest is the “conditional†treatment effect but not the unconditional mean effect. We provide explanations for our rationale of estimating the “conditional†treatment effect. Secondly, we propose a modified downloading variable to replicate Cannon’s analysis. Our results suggest that downloading PowerPoint slides before the exam does not produce a significant effect on absent students’ exam performance which is different from the results in Cannon (2011). Our analysis does support Cannon’s argument that students fixed effects are different across different attendance status.
Mitreola spathulifolia D. Fang & L. S. Zhou
No full textMitreola spathulifolia D.Fang & L.S.Zhou in Fang et al. (1995: 33, Fig. 9–12) (Fig. 4) Literature:— Huang (2007: 79), Chen (2011: 761). Type: — CHINA. Guangxi Zhuang Autonomous Region: Mashan, Guling, in cavern of limestone hill, 310–330 m, 07 June 1994, Fang D., Zhou L. S ., Xie Z. M. 3146 (lectotype, designated here: GXMI: GXMI050420 photo!; isolectotypes: GXMI: GXMI050418 photo!, GXMI050419 photo!, GXMI050421 photo!; GXNM). Images of lectotype and isolectotypes available at https://www.cvh.ac.cn/spms/detail.php?id=09174c08 https://www.cvh.ac.cn/spms/detail.php?id=09174aeb https://www.cvh.ac.cn/spms/detail.php?id=09174b79 https://www.cvh.ac.cn/spms/detail.php?id=09174c92 Nomenclatural notes: —In the original description of Mitreola spathulifolia, Fang et al. (1995) indicated the duplicate of the collection Fang et al. 3146 at GXMI as a holotype, and its duplicate at GXNM as an isotype. However, we have found four duplicates of Fang et al. 3146 in GXMI matching the detailed description and specimen label information included in the protologue. These duplicates should be treated as syntypes in accordance with Art. 9.6 of ICN (Turland et al. 2018). We selected the specimen with the barcode GXMI 050420 as the lectotype following Arts. 9.3 and 9.11 of ICN (Turland et al. 2018) as it represents best the species, having an intact well-developed stem and numerous inflorescences. The other specimens at GXMI (GXMI 050418, GXMI 050419, GXMI 050421), as well as the one at GXNM (not seen), are isolectotypes. Of them, the specimen GXMI 050418 has been labeled in shed. as a “ holotypus ” during or after preparation of the protologue; the plant in this specimen has only the distal portion of the stem.Published as part of Nuraliev, Maxim S., Lyskov, Dmitry F., Kuznetsov, Andrey N., Kuznetsova, Svetlana P. & Fu, Long-Fei, 2023, Novelties in Asian Mitreola (Loganiaceae): M. capitata, a new species from Vietnam, and lectotypification of M. spathulifolia, pp. 39-47 in Phytotaxa 585 (1) on page 44, DOI: 10.11646/phytotaxa.585.1.4, http://zenodo.org/record/767292
Ostrya trichocarpa D. Fang & Y. S. Wang 1983
No full text6. Ostrya trichocarpa D.Fang & Y.S.Wang Guihaia 3 (3): 189 (Fang & Wang 1983). – Type: China, Guangxi, Napo County (那坡县), 百都^社, 弄̔, 1260 m a.s.l., 石山 [rocky hill], 23 Apr. 1981, D. Fang, T.-H. Tan and Z.-G. Wang [³鼎, 覃 德海, 王振刚] 22412 (holo-: GXMI050301). – Additional type material: ibid., D. Fang, M.-X. Lai et al. 25379 (para-: GXMI); ibid., Guanxi Zhuang Auton. Region, Jinxi County, 780 m a.s.l., 17 Nov. 1962, Z.-J. Li and H.-Q Li 1650 (para-: IBK00079836!, IBK00079837!).Published as part of Holstein, Norbert & Weigend, Maximilian, 2017, No taxon left behind? - a critical taxonomic checklist of Carpinus and Ostrya (Coryloideae, Betulaceae), pp. 1-52 in European Journal of Taxonomy 375 on page 35, DOI: 10.5852/ejt.2017.375, http://zenodo.org/record/383854
Yi-Fang Lin Piano Recital Program Notes
No full textThis report is Yi-Fang Lin Piano Recital Program Notes on April., 23, 2019. The two pieces on the program include Piano Sonata in C minor, K.457 by W.A. Mozart, and Piano Concerto No.1 in F-Sharp minor, Op.1 by S. Rachmaninoff. The note will introduce the life of two composers, the compositional background of individual work, and the analysis of the structure, including tonal design and thematic material in each work
The Indefinite Object in Mandarin Chinese. Its Marking, Interpretation and Acquisition
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45144.pdf (Publisher’s version ) (Open Access)Radboud Universiteit Nijmegen, 07 mei 2008Promotores : Muysken, P.C., Fang, L., Hoop, H. deXVI, 241 p
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