2,361 research outputs found
Comparison between effects of Ringer′s and Ringer′s lactate irrigating solutions on corneal endothelium during phacoemulsfication
No full textPurpose
The aim of this study was to compare the effect of phacoemulsification on the corneal endothelium using Ringer′s and Ringer′s lactate as the intraocular irrigating solution.
Setting
Mansoura Ophthalmic Centre, Faculty of Medicine, Mansoura University, Egypt.
Design
Prospective randomized comparative study.
Materials and methods
A total of 102 eyes of 102 patients with senile cataract were randomized to have phacoemulsification with foldable intraocular lens implantation performed using Ringer′s (group 1) or Ringer′s lactate (group 2) as the intraocular irrigating solution. Specular microscopy was done preoperatively, 1 week and 3 months postoperatively to evaluate endothelial cell density (ECD), coefficient of variation (CV), and central corneal thickness. Data were analyzed using the t-test, the Mann-Whitney U-test, and the Pearson correlation coefficient.
Results
Mean ECD was 2532 cells/mm 2 preoperatively, 2345 cells/mm 2 1 week, and 2284 cells/mm 2 3 months postoperatively in group 1, and 2526, 2374, and 2322 cells/mm 2 , respectively, in group 2. There was a statistically significant difference between preoperative ECD and each of 1 week and 3 months postoperative ECD in each group. No significant difference was found between 1-week mean ECD reduction in group 1 (7.35%) and group 2 (6.05%) or between 3-month mean ECD reduction in group 1 (9.69%) and group 2 (8.07%).
Conclusion
Ringer′s and Ringer′s lactate solutions are associated with minimal changes in corneal ECD, morphology, and function during uncomplicated phacoemulsification with foldable intraocular lens implantation in patients with normal endothelial cell counts. There is no clinically significant difference in endothelial cell preservation and polymegathism and corneal swelling between Ringer′s and Ringer′s lactate solutions
Ringer analysis of mAMCase structures
No full textThis directory contains all files required to analyze the mouse AMCase structures using Ringer as presented in Supplemental Figure 7 in the manuscript Díaz et al. (2023).
Structures were analyzed using Ringer. Ringer data was analyzed using Python. Figures were compiled using Adobe Illustrator.
Files included in this directory:
8fXX
- Ringer output files for each mAMCase structure reported.
Figures
- contains .PNG files of Ringer plots for Asp136, Asp138, and Glu140 of each chain of each structure reported.
Contact:
Roberto Efraín Díaz, [email protected]
James Fraser, [email protected]
Scanning electron microscopic study of cold-stored small bowel - Comparison of Euro-Collins and lactated Ringer's solutions
No full textThe increasing use of the small bowel for transplantation necessitates the development of optimum preservation techniques. The aim of our study was to investigate time-related morphological changes of the rat small bowel during preservation of the organ in hypothermic Euro-Collins and Lactated Ringer's solutions. It is the only study comparing these widely used preservation solutions with scanning electron microscopy, which is especially suitable to show the intestinal mucosa, one of the tissues of the intestine most susceptible to ischemia. Small bowels were perfused with either Euro-Collins, Lactated Ringer's or physiological saline solution and placed in respective preservation solutions at 4degreesC for 0, 3, 6 and 12 hours. Biopsy samples were prepared for examination at the light and scanning electron microscopic levels. According to the results of this study we conclude that, even Lactated Ringer's solution showed better tissue preservation than Euro-Collins solution, both preservation solutions are suitable for short term preservation of the small bowel and that further ameliorations of the preservation solutions and/or techniques are required for long term preservation
Interactions between the volume effects of hydroxyethyl starch 130/0.4 and Ringer´s acetate
No full textINTRODUCTION: The turnover of Ringer´s solutions is greatly dependent on the physiological situation, such as the presence of dehydration or anaesthesia. The present study evaluates whether the kinetics is affected by previous infusion of colloid fluid. METHODS: Ten male volunteers with a mean age of 22 years underwent three infusion experiments, on separate days and in random order. The experiments included 10 mL/kg of 6% hydroxyethyl starch 130/0.4 (Voluven™), 20 mL/kg of Ringer's acetate, and a combination of both, where Ringer´s was administered 75 minutes after the starch infusion ended. The kinetics of the volume expansion was analysed by non-linear least- squares regression, based on urinary excretion and serial measurement of blood haemoglobin concentration for up to 420 minutes. RESULTS: The mean volume of distribution of the starch was 3.12 L which agreed well with the plasma volume (3.14 L) estimated by anthropometry. The volume expansion following the infusion of starch showed monoexponential elimination kinetics with a half-life of two hours. Two interaction effects were found when Ringer´s acetate was infused after the starch. First, there was a higher tendency for Ringer´s acetate to distribute to a peripheral compartment at the expense of the plasma volume expansion. The translocated amount of Ringer´s was 70% higher when HES had been infused earlier. Second, the elimination half-life of Ringer´s acetate was five times longer when administered after the starch (88 versus 497 minutes, P <0.02). CONCLUSIONS: Starch promoted peripheral accumulation of the later infused Ringer´s acetate solution and markedly prolonged the elimination half-life
Bell Ringer in Rostov
No full textThis brief interview with the 16 year old bell ringer in the Rostov Kremlin includes English-Russian translation
Splenic implant preservation after conservation in lactated Ringer´s solution
No full textABSTRACT Objective: to evaluate the morphology and function of autogenous splenic tissue implanted in the greater omentum, 24 hours after storage in Ringer-lactate solution. Methods: we divided 35 male rats into seven groups (n=5): Group 1: no splenectomy; Group 2: total splenectomy without implant; Group 3: total splenectomy and immediate autogenous implant; Group 4: total splenectomy, preservation of the spleen in Ringer-lactate at room temperature, then sliced and implanted; Group 5: total splenectomy, spleen sliced and preserved in Ringer-lactate at room temperature before implantation; Group 6: total splenectomy with preservation of the spleen in Ringer-lactate at 4°C and then sliced and implanted; Group 7: total splenectomy and the spleen sliced for preservation in Ringer-lactate at 4°C before implantation. After 90 days, we performed scintigraphic studies with Tc99m-colloidal tin (liver, lung, spleen or implant and clot), haematological exams (erythrogram, leucometry, platelets), biochemical dosages (protein electrophoresis) and anatomopathological studies. Results: regeneration of autogenous splenic implants occurred in the animals of the groups with preservation of the spleen at 4ºC. The uptake of colloidal tin was higher in groups 1, 3, 6 and 7 compared with the others. There was no difference in hematimetric values in the seven groups. Protein electrophoresis showed a decrease in the gamma fraction in the group of splenectomized animals in relation to the operated groups. Conclusion: the splenic tissue preserved in Ringer-lactate solution at 4ºC maintains its morphological structure and allows functional recovery after being implanted on the greater omentum.</div
Splenic implant preservation after conservation in lactated Ringer´s solution
No full textABSTRACT Objective: to evaluate the morphology and function of autogenous splenic tissue implanted in the greater omentum, 24 hours after storage in Ringer-lactate solution. Methods: we divided 35 male rats into seven groups (n=5): Group 1: no splenectomy; Group 2: total splenectomy without implant; Group 3: total splenectomy and immediate autogenous implant; Group 4: total splenectomy, preservation of the spleen in Ringer-lactate at room temperature, then sliced and implanted; Group 5: total splenectomy, spleen sliced and preserved in Ringer-lactate at room temperature before implantation; Group 6: total splenectomy with preservation of the spleen in Ringer-lactate at 4°C and then sliced and implanted; Group 7: total splenectomy and the spleen sliced for preservation in Ringer-lactate at 4°C before implantation. After 90 days, we performed scintigraphic studies with Tc99m-colloidal tin (liver, lung, spleen or implant and clot), haematological exams (erythrogram, leucometry, platelets), biochemical dosages (protein electrophoresis) and anatomopathological studies. Results: regeneration of autogenous splenic implants occurred in the animals of the groups with preservation of the spleen at 4ºC. The uptake of colloidal tin was higher in groups 1, 3, 6 and 7 compared with the others. There was no difference in hematimetric values in the seven groups. Protein electrophoresis showed a decrease in the gamma fraction in the group of splenectomized animals in relation to the operated groups. Conclusion: the splenic tissue preserved in Ringer-lactate solution at 4ºC maintains its morphological structure and allows functional recovery after being implanted on the greater omentum
Sulfur Hexafluoride 20% versus Lactated Ringer′s solution for Prevention of Early Postoperative Vitreous Hemorrhage after Diabetic Vitrectomy
No full textPurpose: To compare the hemostatic effect of sulfur hexafluoride 20% (SF6 20%) with lactated Ringer′s solution for prevention of early postoperative vitreous hemorrhage following diabetic vitrectomy.
Methods: In a prospective randomized clinical trial, 50 eyes undergoing diabetic vitrectomy were divided into two groups. At the conclusion of surgery, in one group the vitreous cavity was filled with SF6 20% while in the other group lactated Ringer′s solution was retained in the vitreous cavity. The two groups were compared for the rate of early postoperative vitreous hemorrhage.
Results: The incidence of vitreous hemorrhage was lower in the SF6 group than the Ringer′s group 4 days (20% vs 68%, P=0.001), 7 days (24% vs 60%, P=0.01) and 4 weeks (16% vs 40%, P=0.059) after vitrectomy.
Conclusion: In comparison with lactated Ringer′s solution, SF6 20% had a significant hemostatic effect especially in the early postoperative period after diabetic vitrectomy and reduced the incidence of vitreous hemorrhage
Identification of the material properties of Al 2024 alloy by means of inverse analysis and indentation tests
No full textThis paper outlines an inverse analysis approach aimed at the identification of the mechanical properties of metallic materials based on the experimental results obtained from indentation tests. Previous work has shown the ill-posed nature of the inverse problem based on the load-penetration curve when dealing with mystical materials, which exhibit identical indentation curves even if possessing different yield and strain-hardening properties. For this reason, an additional measurement is used in the present study as input for the inverse analysis which consists of the maximum pile-up height measured after the indentation test. This approach lends itself for practical applications as the load-penetration curve can be easily obtained from commonly available micro-indenters while the pile-up present at the end of the test can be measured by different instruments depending on the size of the indented area, for example by means of an atomic force microscope or a laser profilometer. The inverse analysis procedure consists of a batch deterministic approach, and conventional optimization algorithms are employed for the minimization of the discrepancy norm. The first part of the paper outlines how the inclusion of both the maximum height of the pile-up and the indentation curve in the input data of the inverse analysis leads to a well-defined inverse problem using parameters of mystical materials. The approach is then applied to real experimental data obtained from three sets of 2024 Al alloy samples prepared using different age-hardening treatments. The accuracy of the identification process is validated against the mechanical properties obtained independently from standard tensile coupon tests. The reported results highlight the advantages of the proposed methodology in identifying the elastic modulus, yield stress and strain-hardening coefficient while minimizing the amount of information required to be collected from the experimental indentation tests
Comparison of corneal changes after phacoemulsification using BSS Plus versus Lactated Ringer`s irrigating solution: a prospective randomised trial
No full textBackground/aims To compare two intraocular irrigating solutions, Balanced Salt Solution Plus (BSS Plus) versus Lactated Ringer`s (Ringer), for the preservation of corneal integrity after phacoemulsification. Methods 110 patients undergoing phacoemulsification were randomised to either BSS Plus (n = 55) or Ringer (n = 55) as the irrigating solution. Patients were examined at baseline and at 1, 8, 15, 30 and 60 days postoperatively. Evaluations included specular microscopy to evaluate endothelial cell density (ECD) and endothelial cell size variability (CV), and corneal pachymetry for central corneal thickness (CCT) measurement. Results Groups were well balanced regarding baseline ECD, CV and CCT (p>0.05). There was no statistically significant difference between ECD reduction in group BSS Plus 13.1 +/- 2.0% and Ringer 9.2 +/- 1.9% (p<0.05) at day 60 or in any study visit. There was no statistically significant difference between CV increase in group BSS Plus 23.0 +/- 3.0% and Ringer 20.2 +/- 4.0% (p<0.05) at day 60 or in any study visit. CCT was significantly increased (p<0.05) at 1, 8, 15 and 30 days postoperatively, returning to baseline at 60 days in both groups. There was no significant difference in CCT increase in both groups at any visit. Interestingly, there were statistically significant correlations between ECD loss and phacoemulsification time (p<0.0001) and ECD loss and irrigation solution volume (p<0.0001) in the Ringer group, but not in the BSS Plus group. Conclusions Ringers solution was similar to BSS Plus for corneal preservation in atraumatic cataract surgery. However, our study demonstrates that there is a trend towards lower postoperative endothelial cell density for surgeries with longer phacoemulsification time and higher irrigation volumes if Ringer is used.Conselho Nacional de Pesquisa (CNPq)[306692/2008-2
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