229 research outputs found
Highly efficient synthesis of [3]rotaxane assisted by preorganisation of pseudorotaxane using bis(crown ether)s
No full textThe tether-assisted synthesis of [3]rotaxane by olefin metathesis has been studied in detail. Bis(crown ether)s, in which two crown ethers are connected by a linker, were threaded onto ammonium salts bearing a terminal olefin to form pseudorotaxanes. The pseudorotaxanes were converted into tethered rotaxanes in the presence of Grubbs catalyst, followed by removal of the linkers to produce [3]rotaxanes in excellent yields. Preorganisation of the two reactive ends has led to a great improvement in the yield of [3]rotaxanes. The ring strain of the tethered rotaxanes and the flexibility of the pseudorotaxanes were responsible for the formation of the tethered rotaxanes
Competences for the management of the basic health unities: Managerss Perception
No full textConsiderando a importância do gerenciamento das Unidades Básicas de Saúde para a melhoria da saúde da população com o enfoque de saúde enquanto qualidade de vida realizamos a presente investigação, tendo como objetivos gerais conhecer o perfil de competências dos atuais gestores das Unidades Básicas de Saúde do Município de São Paulo e analisar se essas competências favorecem ou não a implementação dos novos modelos assistenciais e gerenciais, que contemplam as estratégias que lhe são inerentes. Os objetivos específicos foram: identificar na percepção dos atuais gestores quais são as competências para a gestão das UBS e problematizar se a formação e o preparo específico destes gestores influenciam no modo de operacionalização das estratégias e na dinâmica das equipes envolvidas nos serviços. A pesquisa foi qualitativa e a pesquisadora faz parte da instituição em que a investigação foi realizada. A proposta era provocar mudança de paradigmas nos agentes envolvidos, caracterizando-se dessa maneira uma pesquisa ação. Na primeira fase os dados foram coletados por meio de entrevistas e na segunda, realizamos uma devolutiva das análises das entrevistas, cujas unidades de significado nortearam os temas disparadores para a realização de um encontro de grupo focal. Foram três temas: quais são os conhecimentos gerenciais necessários a um gestor de UBS, quais são as habilidades gerenciais a serem desenvolvidas por um gestor de UBS e quais são as atitudes que devem permear essas habilidades. Dez dos dezenove gerentes da região estudada (52,63%) participaram da pesquisa. Os resultados evidenciaram que a percepção dos atuais gestores em relação às competências para gerir uma UBS contemplam uma visão simplista e que existe um despreparo desses agentes. Isto influencia diretamente o modo de operacionalização das estratégias e a dinâmica das equipes, contribuindo para a ineficácia e ineficiência dos processos.Considering the relevance of managing Basic Health Unities and in improving the populations health understanding health as quality of life this research has had the following objectives: identify the competence profile of the actual managers of the Basic Health Unities of São Paulo City and analyse if this competences favours or not the implementation of new care and managerial models that contemplates inherent strategies. The specific objectives were: identify the perceptions the actual managers have in relation to the competences needed to manage a Basic Health Unity and discuss with them if their knowledge and specific qualifications influences team dynamics and the implementation of the strategies The research was the qualitative mode and the researcher works in the same institution were the research was made and as promoting the discussions about the themes it configures a research- action type. In the first stage, the data were obtained through individual interviews. In the second stage the data were analysed according to the thematic analyses and then showed to the managers as starting themes of a focus group. The themes were: what kind of managerial knowledge should the Basic Health Unity manager have, what kind of managerial abilities should the manager develop, what kind of behavior should come with the managers abilities. Ten of the nineteen managers of the Basic Health Unities (52.63%) in the region studied participated .The results showed that the actual managers perception about the managerial competences that managers should have is simplistic. This agents are not prepared to manage and this influences directly the teams dynamic and contributes for the ineffectiveness and inefficiency of the strategies implementation
Reconstitution of the 9 S estrogen receptor with heat shock protein 90
No full textAbstractAs a first step in the investigation of the reconstitution of steroid hormone receptor systems, we studied the reconstitution of 9 S estrogen receptor (ER) from purified vero ER, which is the estradiol binding subunit, and heat shock protein 90 (hsp 90). By using a phosphate buffer containing molybdate, thiocyanate, dimethylformamide, glycerol, etc., vero ER could be converted to 9 S ER with hsp 90, but not with the control protein, ovalbumin. Inactivation of ER during the reconstitution was suppressed partially by hsp 90, but not by ovalbumin. Like native 8 S ER, the reconstituted ER was sedimented at about 8.9 S and 4.6 S on glycerol gradient centrifugation in low and high salt buffers, respectively
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