849 research outputs found
Sustainable Management of Events in an Experiential Perspective
No full textEvents are complex value propositions that are realized by specialists to meet different leisure and entertainment needs. They are highly intangible and experiential-type products (Pencarelli and Forlani 2016) to which the application of logical experience is particularly suitable (Bartolazzi et al. 2008; Pegg and Patterson 2010; Manthiou et al. 2014; Getz and Page 2016). This chapter aims to describe the role played by events in the experience economy for territories involved in sustainable tourism strategies. The authors also propose a model for event impact analysis and evaluation. Starting from the idea that tourist destinations represent a stage on which tourism products can be displayed, so as to satisfy the demands of travelers seeking engaging experiences (Pencarelli and Forlani 2016), the importance of the role played by destination management in managing the events is further underlined. Tourists searching such experiences want to be in the spotlight and co-participate with territorial players in the creation of value. As such, event management requires the use of tools such as planning, organization, and control in order to design, plan, and put on a balanced program of events while monitoring the impact on value creation for both tourists and regional stakeholders within the perspective of sustainability. This chapter proposes to redefine the experiential perspective of a set of indicators that are useful in holistically evaluating the performance of events in a traditional tri-faceted dimension, i.e., economic, social, and environmenta
Arnaldo Forlani
No full textIl governo Forlani nei primi anni Ottanta del XX secolo, tra scelte politiche e percorsi istituzional
Strategies of ELISA development for pesticide detection
No full textThis review examines some of the most common problems generally faced during the development of immunoenzymatic assays. In particular, the criteria of hapten design either for the preparation of the immunogen or the immunoenzymatic assay reagent are discussed. Examples are given from work carried out for the detection of triazole fungicides, specifically, tetraconazole, hexaconazole, penconazole and propiconazole. It is concluded that ELISA performance with polyclonal antibody is mainly affected by 2 factors: the use of a coating conjugate characterized by a handle different in length and nature from that present in the immunizing conjugate, and by a low hapten/protein molar ratio
[Europa] / Paolo Forlani Veronese...
No full textÉchelle(s) : [1:9 260 000 environ], Scala di miglia 6 [= 3,2 cm
Natural compounds targeting E. Coli WRBA as antibiofilm agents: Virtual screening, design and synthesis of analogs, biological evaluation
No full textResistance to antibiotics has become one of the main concerns of modern medicine. This
phenomenon is worsened by biofilm formation, which frequently leads to treatment failures.1 Hence,
anti-biofilm agents represent useful tools in the treatment of microbial infections, increasing the
effectiveness of antibiotics and preventing resistance mechanisms.
Different studies have demonstrated that the protein WrbA plays a key role in the formation of
biofilm in E. coli. Nonetheless, its specific function is still poorly understood, and only a few inhibitors
are reported in the literature.2
For this reason, our research group has worked towards the
identification of new WrbA inhibitors as antibiofilm agents.
Through a target-based virtual database screening, we identified natural products endowed with a
high affinity towards WrbA; the most promising compounds were purchased for biophysical and
biological evaluation.
Hence, we selected a natural scaffold as a starting point to create a new library of organic molecules
and perform a virtual screening. Finally, five compounds were selected to be synthesized and
subjected to biophysical evaluation.
Both the natural and synthetic compounds were tested on recombinant E. coli WrbA by MicroScale
Thermophoresis (MST), which allowed the determination of their dissociation constants (Kd). Among
the natural compounds, one of them displayed a Kd in the nanomolar range. This value was lower
than those measured for both caffeic and zosteric acid, which are known WrbA inhibitors.3 On the
other hand, the synthesized compounds bound WrbA with Kd values in the low micromolar range.
Based on our encouraging results, the inhibitors were assayed on E. coli and S. aureus to verify their
antibiofilm effect. The outcomes of our study will be presented.
References
1. Antimicrobial resistance https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance (accessed 2022-
05-10).
2. Rossi, F.; Cattò, C.; Mugnai, G.; Villa, F.; Forlani, F.; Pinto, A.; Nuzzo, D. Effects of the Quinone Oxidoreductase WrbA on Escherichia Coli Biofilm Formation and Oxidative Stress. Antioxidants 2021, 10 (6), 919.
3. Cattò, C.; Dell’Orto, S.; Villa, F.; Villa, S.; Gelain, A.; Vitali, A.; Marzano, V.; Baroni, S.; Forlani, F.; Cappitelli, F. Unravelling the Structural and Molecular Basis Responsible for the Anti-Biofilm Activity of Zosteric Acid. PLoS ONE 2015, 10 (7), E0131519
Essays on firm efficiency with imperfectly competitive input markets: an empirical analysis
No full textIn the thesis I exploit an empirical analysis on firm'�s productivity. I relate
the efficiency at plant level with the input market features and I suggest an estimation technique for production function that takes into account firm'�s liquidity
constraints. The main results are three. When I consider services as inputs for manufacturing firm�'s production process, I find that more competition in service
sector affects positively plant�s productivity and export decision. Secondly liquidity constraints are important for the calculation of firm'�s productivity because
they are a second source of firm's heterogeneity. Third liquidity constraints are important for firm'�s internationalizatio
HTLV-1 Infection and Adult T Cell Leukemia Mechanisms of Oncogenesis and Alteration of Immunity
No full textElongation of the catalytic loop of Azotobacter vinelandii rhodanese changed selectivity from sulfur to phosphorus compounds as substrates
No full textThe human genome contains numerous genes whose protein products are unknown in terms of structure, interaction partner, expression, and function. To unravel the function of these orphan genes, it is of particular value to isolate native forms of protein and peptide products derived from these genes. From human blood ultrafiltrate, we characterized a novel gene-encoded, cysteine-rich, and cationic peptide that we termed liver-expressed antimicrobial peptide 2 (LEAP-2). We identified several circulating forms of LEAP-2 differing in their amino-terminal length, all containing a core structure with two disulfide bonds formed by cysteine residues in relative 1-3 and 2-4 positions. Molecular cloning of the cDNA showed that LEAP-2 is synthesized as a 77-residue precursor, which is predominantly expressed in the liver and highly conserved among mammals. This makes it a unique peptide that does not exhibit similarity with any known human peptide regarding its primary structure, disulfide motif, and expression. Analysis of the LEAP-2 gene resulted in the identification of an alternative promoter and at least four different splicing variants, with the two dominating transcripts being tissue-specifically expressed. The largest native LEAP-2 form of 40 amino acid residues is generated from the precursor at a putative cleavage site for a furin-like endoprotease. In contrast to smaller LEAP-2 variants, this peptide exhibited dose-dependent antimicrobial activity against selected microbial model organisms. LEAP-2 shares some characteristic properties with classic peptide hormones and it is expected that the isolation of this novel peptide will help to unravel its physiological role
The effects of formal networks on territorial tourism offers. Current usage of network contracts in Italy
No full textThis study aims to understand the benefits contractual inter-firm networks offer to existing territorial tourism systems in Italy. Preference has been given to a qualitative approach. Data collection was based on document analysis (i.e. information reported in written contracts as well as text and images published on web sites) and in-depth semi-structured interviews. Results indicate that usage of a relatively new legal mechanism (the network contract) designed to form stable inter-organisational networks is increasing in the tourism sector. These networks help promote existing territorial tourism systems in different ways and to different extents. In some cases, alliances create and promote a tourism proposal that enhances the territorial offer, while in other situations alliances succeed in identifying and communicating a specific territorial area previously left unmanaged
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