1,720,974 research outputs found
UJI AKTIVITAS ANTIBAKTERI RIMPANG KUNYIT PUTIH (Kaempferia rotundaL.) TERHADAP Streptococcus sobrinus DAN Salmonella typhi
Full text linkAn infectious disease caused by bacteria is a disease that often occurs in tropical areas. The used of plant extract is an interesting object of study to overcome these infectious diseases and because of an increase in bacterial resistance to antibiotics. White turmeric rhizome widely used by Indonesia society as an herb which has been used for the treatment of diarrhea and abdominal pain. This study aims to determine the content of secondary metabolities and inhibitory of white turmeric rhizome (Kaempferia rotunda L.) against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. The results of phytochemical screening white turmeric rhizome contained compound triterpenoids, steroids, phenolics, and saponins. An antibacterial activity test used agar diffusion method against Streptococcus sobrinus KCCM 11898 and Salmonella typhi ATCC 422 bacteria. Antibacterial activity white turmeric rhizome (Kaempferia rotunda L.) with concentration 1, 2, 4, 8% have an inhibition zone diameter against Streptococcus sobrinus KCCM 11898 bacteria is 10.33, 10.67, 17 and 25.17 mm respectively with the antibacterial strength is strong, meanwhile in Salmonella typhi ATCC 422 bacteria have an inhibition zone diameter was 8, 8.3, 11.83 and 13 mm respectively with the antibacterial strength is intermediate.
Keywords: Antibacterial, White Turmeric Rhizome (Kaempferia rotunda L.), Agar Difussio
POTENSI AKTIVITAS ANTIBAKTERI EKSTRAK DIKLOROMETANA DAN METANOL BUNGA TEROMPET EMAS (Allamanda cathartica L.) TERHADAP BAKTERI Staphylococcus aureus ATCC 25923
Full text linkPotential antibacterial activity of dichloromethane and methanol extracts of the golden trumpet flower (A.cathartica L.) against S. aureus ATCC 25923 has been conducted. This study aimed to determine the values of thedichloromethane and methanol extracts of A. cathartica L. The antibacterial activity test was conducted using agardiffusion method and carried out in triplicate. The obtained results from the dichloromethane extract and methanol extractof the golden trumpet flower (A. cathartica L.) showed antibacterial activity with inhibition zone diameters of 9.66 mmfor dichloromethane extracts and 8.66 mm for methanol extracts against S. aureus ATCC 25923 bacteria.Potensi aktivitas antibakteri dari ekstrak diklorometana dan metanol bunga terompet emas (A. cathartica L.) terhadap S. aureus ATCC 25923 telah dilakukan. Penelitian ini bertujuan untuk menentukan nilai-nilai dari ekstrak diklorometana dan metanol A. cathartica L. Uji aktivitas antibakteri dilakukan menggunakan metode difusi agar dan dilakukan secara tiga kali ulangan. Hasil yang diperoleh dari ekstrak diklorometana dan ekstrak metanol bunga terompet emas (A. cathartica L.) menunjukkan aktivitas antibakteri dengan diameter zona hambatan masing-masing sebesar 9,66 mm untuk ekstrak diklorometana dan 8,66 mm untuk ekstrak metanol terhadap bakteri S. aureus ATCC 25923
SKRINING FITOKIMIA DAN UJI TOKSISITAS MENGGUNAKAN BRINE SHRIMP LETHALITY TEST (BSLT) DARI EKSTRAK DIKLOROMETANA RIMPANG TEMU KUNCI (Boesenbergia rotunda)
No full textThe rhizome of Boesenbergia rotunda (temu kunci) is one of medicinal plants having potential for medicinal, supplementary food and drink due to its biological activities. The aims of this research are to identified the secondary metabolite compounds present in the dichlorometane extract of finger-root rhizomes through phytochemical screening using thin layer chromatography (TLC) method and to investigated its toxicity against Artemia salina L. shrimp larvae by using Brine Shrime Lethality Test (BSLT). The result of phytochemical analysis showed that the dichloromethane extract contains secondary metabolite compounds including flavonoids, phenolics and triterpenoids while the toxicity test of the extract showed its LC50 value of 303.68 µg/mL. It was indicated that the extract could be classified as toxic
SKRINING BAKTERI ENDOFIT PENGHASIL AMILASE, LIPASE DAN PROTEASE DARI DAUN Syzygium myrtifolium
Full text linkEndophytic bacteria are microorganisms that throughout or part of their life cycle inside plants and will not appear certain symptoms on the plant itself. Endophytic bacteria can produce enzymes. Enzymes produced by bacterial endophytes are more beneficial because the production time is faster. One of the plants that contain bacteria is Pucuk Merah (Syzygium myrtifolium). This study aims to isolate endophytic bacteria from leaves of Pucuk Merah (Syzygium myrtifolium) and to screen these endophytic bacteria as amylase, lipase and protease producers. The results of this study were obtained there are 24 isolates of endophytic bacteria from Pucuk Merah leaves. A total of 19 bacterial isolates from 24 bacterial isolates produce amylase, 1 isolate produce lipase and 24 isolates produce protease.
Keywords: Endophytic bacteria, Syzygium myrtifolium, Amylase, Lipase, ProteaseBakteri endofit merupakan mikroorganisme yang menghabiskan seluruh atau sebagian dari siklus hidupnya di dalam tanaman dan tidak mengakibatkan gejala tertentu pada tanaman inangnya. Bakteri endofit dapat menghasilkan enzim. Enzim yang dihasilkan dari bakteri endofit lebih menguntungkan karena waktu produksinya lebih cepat. Salah satu tanaman yang berpotensi mengandung bakteri yaitu tanaman Pucuk Merah (Syzygium myrtifolium). Penelitian ini bertujuan untuk mengisolasi bakteri endofit dari daun Pucuk Merah (Syzygium myrtifolium) dan menskrining bakteri-bakteri endofit tersebut sebagai penghasil amilase, lipase dan protease. Hasil penelitian ini yaitu diperoleh sebanyak 24 isolat bakteri endofit daun Pucuk Merah. Sebanyak 19 isolat bakteri dari 24 isolat bakteri tersebut menunjukkan positif menghasilkan amilase, sebanyak 1 isolat menunjukkan positif menghasilkan lipase dan sebanyak 24 isolat menunjukkan positif menghasilkan protease.
Kata Kunci: Bakteri endofit, Syzygium myrtifolium, Amilase, Lipase, Protease
 
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
SKRINING AKTIVITAS ANTI Escherichia coli ATCC 25922 EKSTRAK METANOL DARI BEBERAPA TANAMAN RIMPANG
Full text linkAntibacterial activity from methanol extracts of edible rhizomes namely temu putih (Curcuma zedoaria), temu ireng (Curcuma aeruginosis), temu giring (Curcuma heyneana) and temu kunci (Boesenbergia pandurata) against Escherichia coli ATCC 25922 based on diameter of inhibition zone by using agar difusion method was conducted. With 1% of each extracts, methanol extract of temu kunci showed excellence acntibacterial acitivty against E. coli with the diameter of inhibition zone of 20,0 mm and followed by methanol extract temu giring is 19,0 mm. While methanol extract of temu ireng and temu putih revealed strong antibacterial activity with inhibition zones of 10.7 and 10,0 mm, respectively.
Keywords: Methanol Extract, Antibacterial, Escherichia col
ENZYMATIC HYDROLYSIS WITH Saccharomyces cerevisiae FOR ETHANOL PRODUCTION FROM MULI BANANA PEEL (Musa acuminata Linn)
Full text linkEthanol production from starch of muli banana peel by enzymatic hydrolysis with the addition of gelatin as a fermentation nutrient for Saccharomyces cerevisiae was done. This research consists of hydrolysis, fermentation, distillation and testing carried out by Gas Chromatography. This research was conducted to determine the optimal time and the optimum nutrient concentration, that can be seen that the optimum time at 7 days and the optimum nutrient concenctration in addition of 2% nutrient with ethanol concentration of 36.344%
AKTIVITAS ANTI-Staphylococcal SECARA IN VITRO EKSTRAK DIKLOROMETANA DARI RIMPANG TEMU KUNCI (Boesenbergia rotunda) TERHADAP Staphylococcus aureus ATCC 25923
Full text linkThe rhizome of temu kunci (Boesenbergia rotunda) is one of the plants used by the local people in Indonesia as a traditional medicine. This study aimed to identify the secondary metabolite compounds contained in the dichloromethane extract with phytochemical screening and investigate its antibacterial activity against test bacteria Staphylococcus aureus ATTC 25923 was carried out by agar diffusion method with modification where tetracycline as a positive control and ethanol as a negative control. The phytochemical screening showed the dichloromethane extract contains secondary metabolites such as alkaloids, flavonoids, phenolics and triterpenoids. With 1 mg/mL of dichloromethane extract revealed its antibacterial activity against S. aureus with inhibitory zone value of 15.0 ± 0.0 mm while its MIC value of the extract was 0.031 ± 0.0 mg/mL toward of tested bacteria. In addition, tetracycline as a positive control at 0.5 μg/mL exhibited its antibacterial potential with an inhibitory zone value of 22.0 ± 0.0 mm and its MIC value of 0.016 ± 0.577 µg/mL.The rhizome of temu kunci (Boesenbergia rotunda) is one of the plants used by the local people in Indonesia as a traditional medicine. This study aimed to identify the secondary metabolite compounds contained in the dichloromethane extract with phytochemical screening and investigate its antibacterial activity against test bacteria Staphylococcus aureus ATTC 25923 was carried out by agar diffusion method with modification where tetracycline as a positive control and ethanol as a negative control. The phytochemical screening showed the dichloromethane extract contains secondary metabolites such as alkaloids, flavonoids, phenolics and triterpenoids. With 1 mg/mL of dichloromethane extract revealed its antibacterial activity against S. aureus with inhibitory zone value of 15.0 ± 0.0 mm while its MIC value of the extract was 0.031 ± 0.0 mg/mL toward of tested bacteria. In addition, tetracycline as a positive control at 0.5 μg/mL exhibited its antibacterial potential with an inhibitory zone value of 22.0 ± 0.0 mm and its MIC value of 0.016 ± 0.577 µg/mL
SKRINING AMILASE, LIPASE DAN PROTEASE DARI BAKTERI ENDOFIT DAUN CIPLUKAN (Physalis angulata L.)
Full text linkEndophytic bacteria is one of the source of extracellular enzymes producer. Enzymes that produced by endophytic bacteria are more beneficial because they can be cultured in small spaces with big quantities, relatively fast time and cheaper production costs compared to the sources of enzyme from plants and animals. This study was conducted to obtain amylase, lipase and protease from endophytic bacteria of ciplukan leaves (Physalis angulata L.). The research method used is experimental, by isolating endophytic bacteria from ciplukan leaves (Physalis angulata L.) in Nutrient Agar (NA) media and screening tests of extracellular enzyme activity from endophytic bacteria isolates. The isolation results was obtained 24 endophytic bacteria isolates that 4 bacteria isolates produce amylase, 3 bacteria isolates produce lipase and 2 bacteria produce protease. Bakteri endofit adalah salah satu sumber penghasil enzim ekstraseluler. Enzim yang dihasilkan oleh bakteri endofit lebih menguntungkan karena dapat dikulturkan dalam ruang kecil dengan jumlah yang besar, waktu yang relatif cepat dan biaya produksi yang lebih murah dibandingkan dengan sumber enzim dari tumbuhan maupun hewan. Penelitian ini bertujuan untuk menentukan potensi bakteri endofit dari daun ciplukan (Physalis angulata L.) sebagai penghasil enzim ekstraseluler (amilase, lipase dan protease). Metode penelitian yang digunakan yakni eksperimental, dengan mengisolasi bakteri endofit dari daun ciplukan (Physalis angulata L.) pada media Nutrient Agar (NA) dan selanjutnya dilakukan uji skrining aktivitas enzim ekstraseluler dari isolat bakteri endofit. Hasil isolasi bakteri endofit yang diperoleh adalah 24 koloni tunggal dengan 4 isolat bakteri menghasilkan amilase, 3 isolat bakteri menghasilkan lipase dan 2 bakteri menghasilkan protease
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