1,721,042 research outputs found
(27(3):223-227)Studies on the prodution of mung bean sprouts with the plant hormone
No full text本研究之主要之目的為探討使用適當植物荷爾蒙栽培綠豆芽，以提高產量和品質。依外觀觀察，綠豆芽彎鈎部位可作鑑定處理別之檢定關鍵•蛋白質測定以Ethrel (2-chforoethyl phosphonic acid)及2,4-D (2,4-Dichloro phenoxyacetic acid)之處理均可提高蛋白質含量。掃描式電子顯微鏡觀察顯示，Ethrel及2,4-D均可使下胚軸之皮層細胞擴大。本文建議綠豆芽以Ethrel 10 ppm處理具有商業利用價值且無藥劑殘餘量對人體健康可謂無虞。
A study for promoting quality and yield mung bean sprout cultivation with the excellent plant hormone.
Morphological observation showed that the hook of mung bean sprouts is the key to identifying of treatments. It is showed that the key is the method of biological test.
Protein analysis showed that Ethrel and 2,4-D treatments increased the cotent of mung bean sprouts.
Scanning electronic microsopy showed that Ethrel treatment and 2,4-D treatment induced the enlargement of cortex cell of the hypocotyl section.
It is suggested that mung bean sprout treated with 10 ppm of Ethrel should be valliable in the commercial production of mumg bean sprouts, proccided that residue would not be a problem for human health
(41(2):178-186)環氨基丙烷羧酸及益收素對試管內蝴蝶蘭苗之生理影響
No full textPhalaenopsis plantlets in 50.0ml flask received 0, 2, 5, 10, 20 ppm Ethrel and 0.01, 0.1, 1 mM 1-aminocyclopropane-1-carboxylic acid (ACC) to study the physiological influence of Ethrel and ACC through culturese in vitro. Ethylene was produced by either the addition of Ethrel or ACC in flask. The treatment of 5.0, 10.0 ppm Ethrel shortened and swelled the roots and produced vigorous plantlets which took the advantage of survival and less mechanical injury during transplant from flasks. However, 20ppm Ethrel induced overdosage effect. The treatment of 0.1 mM ACC produced good quality plantlets as well as 5.0, 10.0 ppm Ethrel. The addition of 1 mM ACC caused small, curl leaf and shorter roots which presented the deteriorated effect on damage of roots and stunted growth of plantlets. The ACC content in different parts of plantlet was determined. The higher ACC residue was existed in root than in central young leaf and older leaves.
蝴蝶蘭幼苗於50毫升三角瓶內以0，2.5，5，10，20 ppm的益收素（Ethrel）及0.01，0.1，1mM的環丙烷氨基羧酸（1-aminocyclopropane-1-carboxylic acid，ACC）以研究二者對蝴蝶蘭幼苗之生理影響。二者均可產生乙烯。試驗結果顯示以益收素5.0及10.0 ppm可以使根部縮短肥大，植株健旺，有利於瓶苗移植減少操作時機械傷害。但是益收素濃度在20 ppm時則有藥劑過量現象。以0.1mM的環丙烷氨基羧酸亦得到與益收素5.0及10.0ppm處理效果一樣優良瓶苗，處理濃度在1mM時造成葉片變小、捲葉、根短、顯示了有害於根部及阻礙生長的現象。環丙烷氨基羧酸之含量分別於不同部位檢測，根部留存量較高於葉片部分
(31(3):220-224)Studies on Tissue Culture of Cactus (Gymnocal ycium minansvichii Var.)
No full text含有4 mg / 12,4-dichlorophenoxyacetic acid（2,4-D）及1mg / l 6-furfurylamin – opurine（Kinetin）之Gamborg（3）修正配方培養基培養緋牡丹仙人掌（Gymnocalycium minansvichii Var.）的仔球不定芽組識可達到大量繁殖的目的。9,000 lux 之照光有利於Callus 之形成，黑暗下形成之Callus 呈白化現象。將Callus 培養於含有10 mg / 1 Naphthalenecetic acid ( NAA ) 及5 mg / l Kinetin 之Gaoborg 修正配方培養基可誘導器官之分化。
Mass In Vitro propagation of red cactus (Gymnocalycium minansvichii Var. )can be achieved by culturing auxilary bud of plantlets on modified Gamborg medium containing 4 mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 1 mg/l furfurylaminopurine (Kinetin). Light treatment 9,000 lux in intensity promoted more callus formed. Calli formed in dark showed albinism. Modified Gamborg medium used for organogenesis contains 10mg/l naphthaleneacetic acid (NAA) and 5mg/l kinetin
(30(2):141-145)Studies on the colonal propagation of Phalaenopsis by root culture
No full text本研究在探討蝴蝶蘭根組織培養明確之配方。結果摘錄如下：1. 蝴蝶蘭根經B5無機鹽及有機鹽，100 mg / 1 myo-inositol, 309 / l 蔗糖，5 mg / 1 Naphthaleneacetic acid ( NAA ) , 10mg / 16 -furfurylaminopurine ( Kinetin )的培養基（pH 5.5）誘導，得癒合組織及芽球相似物突起。2. 芽球相似物繼代培養於相同配方的固態培養基6個月後形成蝴蝶蘭幼植株。3. 芽球相似物僅能在根尖部位形成。4. 照光與未照光處理對芽球相似物之誘導均未有顯著之影響。This paper describes an efficient method of clonal propagation through Phal aenopsis root cuitued in vitro. The result as summaried as follows:
Calli and protocorm like bodies (PLB) were induced from root tip by using Gam borg’s B5 basal medium with 100mg/l myo-inositol, 30,000mg/l sucrose, 5mg/l NAA and 10mg/l kinetin. The pH was adjusted to 5.5. In similar solid medium, the PLB were then developed into baby phalaenopsis plants in 6 months. The formation ability of PLB was only on root tip. There is no significant variation between light and dark treatment
(29(3):195-201)Studies on the clonal propaganion of Phalaenopsis by lef tissue culture
No full text本研究在探討蝴蝶蘭葉片組織培養明確之配方。1.蝴蝶蘭經B5無機鹽及有機鹽，myo – inositoll00 mg / l，蔗糖30 g/ 1 , agar 1%, NAA 5 mg / 1 , kinetin 10 mg / l 的培養基（pH 5.5）誘導，得癒合組織及芽球相似物突起。經在相同培養基作繼代培養可使分化成幼苗。2.葉組織培養中，以心葉形成芽球相似物之能力為最高，而在上表皮形成率較下表皮形成率為高。3.由組織切片顯微鏡觀察，芽球相似物之形成是由表皮細胞及其下面細胞分裂而來。同時亦發現維管束鞘附近薄壁細胞，細胞核／細胞質比例大，具有細胞分裂活性。
An investigation of finding the clear and good media were carried out in order to establish the method of clonal propagation by leaf tisue culture in Phalaenopsis.
Calli and protocorm-like bodies (PLB)were induced from leaf tissue Phalaenopsis of in a basal medium containing Gamborg’s B5 salts, 100 mg/l of myoinositol, 30 g/l of sucrose, 10 g/l of agar, 5 mg/l of NAA and 10 mg/l of kinetin, the pH was adjusted to 5.5. Plantlets were also induced by subculture in the same medium.
The ability of PLB formation was higher in the first than in the second and third leaves of ths explant of infant seedling. It was obssrved that PLB occured most frequently on the adaxial rather than the abaxial surface of the leaves.
Studies by light microscopy indicated that PLB formed might be deried from cells just below the epidermal layer. This is further demonstrated by the high nucleus/cytoplasm ratio and active cell division of the parenchyma cells in the bundle sheath tissues
(40(4):417-424)環丙烷基羧酸與益收素對試管內非洲菊之影響
No full textGerbera (Gerbera jamesonii, Bolus) plantlets in 125 ml flask received 0, 2.5, 5, 10, 20 ppm Ethrel and 0.01,0.1,1 mM 1- aminocyclopropane-1- carboxylic acid (ACC) to study the effect of ethylene and ethylene biosynthesis through cultured in vitro. The good quality plantlets which had more adventitious roots, no overgown stem, no etiolated plantlets by the addition of 5, 10 ppm Ethrel. However, 20 ppm Ethrel caused etiolation of leaves and less roots. This result shows that 20 ppm Ethrel for gerbera plantlets was overdsage. The addition of ACC induced the deteriorated effect on the growth of plantlets. The damage of roots and stunted growth were observed in ACC treatment.非洲菊（Gerbera jamesonii, Bolus）幼苗於試管內處理以0, 2.5, 10, 20 ppm的益收素（Ethrel）及0.01, 0.1, 1 mM的環丙烷氨基羧酸（l-aminocyclopropane-l-carboxylic acid, ACC）以研究乙烯對試管苗之影響及乙烯生合成，結果以5及10ppm的益收素處理能得到良好品質的苗，為多側根不徒長，不白化的幼苗。但是20 ppm的益收素造成葉片白化根數少之過量危害。ACC的作用（註ACC是乙烯之中間先驅物質），造成對幼苗不利於生長，根部危害甚大停止生長。此現象顯現於全部ACC的處理
Phalaenopsis Plantlet Handling Techniques
No full text裸根會造成蝴蝶蘭Phalaenopsis amabilis 及白花黃心品系(Phal. Asian Elegance × Phal.
Taisuco Sheen ‘White flower’)乙烯生成量及呼吸率快速提升。黑暗貯運出庫之後，葉片外觀
略失水軟化，根部呈現乾癟、木栓化等現象產生，但真正嚴重影響是在種植復水之後。當
暗處理出庫後給予復水可促使ACC oxidase 活性提升，大白花品系下位葉、外側根及內側
根與Phal. amabilis 的外側根均可測得酵素活性顯著的變化。大白花以下位葉19.29
nl/g.FW.hr 提升最高，蝴蝶蘭Phal. amabilis 則是外側根33.62 nl/g.FW.hr 變化較大。此活性
的改變促使ACC 快速氧化成乙烯，造成復水後乙烯含量的上升，導致下位葉黃化，根部活
性降低。進而造成各種生理現象的改變。試以藥劑處理後，分析出庫與種植7 天後蝴蝶蘭
各種生理性狀，以浸漬蘋果酸100 mM 及脯胺酸5 mM 效果較佳。無論葉綠素、蘋果酸、
可溶性糖含量以及根部活性各方面均可減少其損失，且於之後種植期間有較佳生育活力。
Bare root would make Phalaenopsis amabilis and Phal. hybrid ‘White flower’
(Phal. Asian Elegance × Phal. Taisuco Sheen) ethylene production and respiration rate
increased quickly. After simulated dark transport, water loss and droop were found in
leaves appearance, and roots became inactivity resulted in dry and shrinkage and
suberizated. The most serious influence was following rehydration. Rehydration could
make ACC oxidase activation after simulated dark transport. Measuring on enzyme
activity in basal leaf, internal and external root of ‘White flower’ and internal root of Phal.
amabilis became more vigority. The most higher enzyme activity of ‘White flower’ on the
basal leaf was 19.29 nl/g.FW.hr, Phal. amabilis on external root was 33.62 nl/g.FW.hr.
The changes of enzyme activity made ACC oxidized into ethylene quickly. Rehydration
made ethylene production arised and led to yellow of basal leaf, set down root activity.
Those results made more physiological changes successively. The changes of
physiological properties of phalaenopsis which treated with chemicals from departure of
storage room and transplanted for 7 days. Soaking in malate 100 mM and proline 5 mM
made much more effect. After drenching malate and proline chemicals, chlorophyll,
malate, total soluble sugar content and root activity had better works, and then could get
more superior in nursery plant vigority after transplanted
Effects of Chemical Pretreatment on the Senescence Physiology of Cut Rose after Dry Cold Storage
No full text本試驗利用黛安娜‘Noblesse’玫瑰切花商業栽培品種作為試驗材料，調查低溫乾藏後關鍵老化機制之探討，並針對冷藏後生理的劣變研發利用適合之藥劑加以改善。玫瑰切花經由100 ppm Agral-LN 預措對於貯藏切花鮮重之維持僅於瓶插初期有效用，10 mM proline 及
10 mM CaCl2 處理皆可以提升切花貯藏後之吸水能力並促進花朵的開放。切花經低溫貯藏後常會造成膜體性質的改變、乙烯的生成及離子滲漏率的增加，使用 CaCl2 預措則可顯著的抑制ACO 的活性，進而降低乙烯的生成，並且有降低膜體微黏稠度的效果，尤其可延緩8℃貯藏之切花細胞微黏稠度的提升，而接近4℃貯藏者之變化趨勢，並藉由穩定膜體完整性，達到改善貯藏後切花品質之目的。
Cut flower of Rosa Hybrida L. cv. ‘Nobless’ was studied to investigate the crucial
aging mechanism after dry cold storage to improve the deteriorated damage cold storage
flowers resulted from disorder physiological changes of cut roses. The cut flowers pulsed
with 100 ppm Agral-LN, could only maintain its fresh weight at the early stage in vase.
Cut rose pulsed with 10 mM proline and 10 mM CaCl2 could enhance the water uptake
capability and promote the flower opening after cold storage. The cold storage cut roses
existed membrane characteristic changes, increase of ethylene production and electrolyte
leakage. Consequently, flowers pulsed with CaCl2, the ACO activity could be inhibited
significantly, the ethylene production could be reduced, and the membrane microviscosity
was less than no treatment had. As a result the cut flowers handled with 8°C storage
could delay the increase trend of microviscosity and its pattern was similar to that cut
roses handled with 4°C storage. In terms of stability of membrane integrity through
delayed membrane microviscosity on rising trend, the accomplishment of quality
improvement of flowers which suffered cold stored came true
(44(4):483-498)Application of the Greenhouse Environmental Model on the Protected Culture for Floriculture
No full text在此研究中，以溫室內部微氣候模式評估國內花卉業者所使用之三型溫室其環控性能與降溫極限。玻璃溫室之熱累積十分嚴重因此必須以大量之機械通風方能克服。開放型塑膠布溫室則可疏解此類問題。國內溫室以蒸發冷卻技術在夏季不能符合蝴蝶蘭催花需求，使用冷凍機械為唯一選擇但增加投資成本。利用遮蔭網或遮雨棚均無法提供觀賞作物經年生產所需之微氣候。而以溫室之結構與控制設備可加以改善內部環境但將增加成本。因此必須以整合方式進行溫室作物栽培生產規劃作業。In this study, the enviromental control performace of three types of greenhouses for floriculture in Taiwan were evaluated by the greenhouse microclimate models. The heat accumulation of glasshouse were so serious that it required the highly volume ventilation. The open-type plastic house could severed as good protected structures to releave the heat accumulation problems. The temperatures in greenhouse cooled by evaporative cooling technique could not meet the requirement of controlling flowering of Phalaenopsis plants. The refrigeration equiupments was the only choice but the investment would be too expensive. The shading net and rain shelter used popularly could not maintain the adequate climate for ornamental culture for all the year round in Taiwan. The microclimate could be improved by the adequate structures and control devices of greenhouse, but it would increase the production cost. The integrated plan of the several factors is very important to execute the production plan of greenhouse
Effects of Growth Retardant, Calcium Nitrate and Pinching on the Quality of Pot Poinsettia
No full text本試驗以聖誕紅商業栽培品種‘倍利’（‘Pepride’）、‘自由’（‘Eckespoint Freedom’）與‘聖誕玫瑰’（‘Winter Rose’）插穗為試驗材料，以探討不同濃度之生長抑制劑、硝酸鈣及5種摘心方式處理對聖誕紅盆花品質之影響。
隨著paclobutrazol處理濃度增加，此三品種聖誕紅株高、乾鮮重、枝梢生長量、葉面積皆有降低之趨勢，而花期則有提早之現象。‘倍利’、‘自由’與‘聖誕玫瑰’在80 ppm處理濃度下，株高只有對照組的50％左右，到花日數則提早3∼16天。‘聖誕玫瑰’對paclobutrazol較‘倍利’與‘自由’敏感，paclobutrazol處理濃度越高，葉片向內捲曲越嚴重，但對苞葉Lab值與葉綠素含量有促進之效果。隨著paclobutrazol處理濃度增加，植株含氮量亦有提高之現象，其中以‘倍利’ 80 ppm處理濃度下含氮量3.3％最高。且營養生長期植株氮素含量較高，轉色期後隨之降低，至盛花期時其含氮量最低。植株中大量元素的含量，會隨paclobutrazol濃度提高而增加，微量元素含量則無固定之趨勢。
隨著硝酸鈣處理濃度的增加，‘倍利’、‘自由’與‘聖誕玫瑰’之株高、枝梢生長量、花序直徑與地上部鮮重等均會隨之增加， ‘倍利’以 300 ppm處理之植株高為16.83 cm較未處理硝酸鈣者11.67 cm多了5 cm左右，但以400 ppm Ca(NO3)2 處理時，‘倍利’之株高與地上部鮮重則呈現下降趨勢。‘倍利’苞葉之Lab值會隨硝酸鈣濃度增加而增加，而‘自由’苞葉之明亮度則會隨之下降。以Ca(NO3)2處理時可促進‘自由’與‘聖誕玫瑰’在盛花期時之葉片葉綠素含量，但對植株之碳水化合物含量則無明顯之影響。
‘倍利’、‘自由’與‘聖誕玫瑰’以弱摘心後立即去除葉片葉柄之處理方式（D與E），可獲得較矮之植株與較多的花序數目，而單獨弱摘心或弱摘心後待側芽萌發1 cm去除葉片葉柄之處理（A、B與C），其植株側枝較長但數量較少，而葉柄的去留對植株之影響差異不大。‘聖誕玫瑰’ 以弱摘心後立即去除葉片葉柄後，其株高為22.43 cm，花序數目為5.33個，較單獨弱摘心矮4 cm並多了2個花序。而不同摘心處理方式對聖誕紅植株葉綠素與碳水化合物含量之影響則無明顯之差異。Euphorbia publcherrima Willd. ex ‘Pepride', ‘Eckespoint Freedom' and ‘Winter Rose' were used in this study. The objective is to investigate the effect of growth retardant, Ca(NO3)2 treatment and pinching types on the quality of poinsettias.
The plant height, dry weight, fresh weight, growth of shoots and leaves area of three cultivar were decreased along with the concentration of paclobutrazol, but anthesis were apeared earlier. When ‘Pepride', ‘Eckespoint Freedom' and ‘Winter Rose' were treated with 80 ppm resulted in plant were half height of controlled plant and anthesis were apeared earlier about 3~16 days. ‘Winter Rose' were more sensitive to paclobutrazol than ‘Pepride' and ‘Eckespoint Freedom'. The leaves of ‘Winter Rose' curled seriously but the values of L, a, b and chlorophyll content would increase along with the concentration. The macroelements content of nitrogen, phosphorus, potassium, calcium and magnesium of three cultivars would increase with treated concentration.
Three cultivar of poinsettia were treated with different Ca(NO3)2 concentration, on which plant height, growth of shoots, infloresence diameter and fresh weight would increase along with the concentration. The height of ‘Pepride' treated with Ca(NO3)2 300 ppm was 16.83 cm more than 0 ppm 5 cm. However the plant height and fresh weight of ‘Pepride' would decrease at 400 ppm. The values of L, a, b of ‘Pepride' bracts would increase with concentration, but the brightness of ‘Eckespoint Freedom' bracts decreased. Nevertherless increased the chlorophyll content of ‘Eckespoint Freedom' and ‘Winter Rose' at anthesis, but had no effects on the carbohydrate content of poinsettias.
‘Pepride', ‘Eckespoint Freedom' and ‘Winter Rose' were treated with soft pinching with leaf and petiole removal (D and E), resulted in shortening the plant height but had more 1~2 infloresences. Only soft pinching or soft pinching with leaf and petiole removal after lateral shoot 1 cm long (A, B and C) could increase the long of lateral shoots but the number of lateral shoots decreased. ‘Winter Rose' were treated with soft pinching with leaf and petiole removal the plant height was 22.43 cm and the number of infloresences was 5.33. Three cultivars of poinsettia were treated with different pinching types had no effects on the chlorophyll and carbohydrate content.壹、 前言（Introduction）……………….…………………….1
貳、 前人研究（Literature review）………….………………..3
參、 材料與方法（Material and methods）………………….20
肆、 結果（Results）……………………………………….…27
伍、 討論（Discussion）……………………………...……….88
陸、 中文摘要（Chinese Summary）…………………………96
柒、 英文摘要（English summary）…………..…………….98
捌、 參考文獻（References）……………..…….….………..99
玖、 附錄……………………………………..…………...10
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