1,720,981 research outputs found
Omega-3 fatty acids increase the unfolded protein response and improve amyloid-β phagocytosis by macrophages of patients with mild cognitive impairment
No full textMacrophages (Mφs) of patients with Alzheimer's disease and mild cognitive
impairment (MCI) are defective in amyloid-β1-42 (Aβ) phagocytosis and have low
resistance to apoptosis by Aβ. Omega-3 fatty acids (ω-3s) in vitro and in vivo
and the ω-3 mediator, resolvin D1, in vitro increase Aβ phagocytosis by Mφs of
patients with MCI. We have investigated the unfolded protein response (UPR) to
endoplasmic reticulum (ER) stress by Mφs in a longitudinal study of fish-derived,
ω-3-supplemented patients with MCI. Patients in the apolipoprotein E (ApoE)e3/e3
subgroup over time exhibited an increase of protein kinase RNA-like ER kinase
(PERK) expression, Aβ phagocytosis, intermediate M1-M2 Mφ type, and a Mini-Mental
State Examination (MMSE) rate of change of +1.8 points per year, whereas patients
in the ApoEe3/e4 subgroup showed individually divergent results with an MMSE rate
of change of -3.2 points per year. In vitro treatment of Mφs by fish-derived ω-3
emulsion increased Aβ phagocytosis, PERK expression, and UPR RNA signature, and
decreased ER stress signature. Augmented genes in the UPR signature included
chaperones, lectins, foldases, and N-linked glycosylation enzymes. In summary,
fish-derived ω-3s increase cytoprotective genes and decrease proapoptotic genes,
improve immune clearance of Aβ, and are associated with an improved MMSE rate of
change in ApoEe3/e3 vs. ApoEe3/e4 patients.-Olivera-Perez, H. M., Lam, L., Dang,
J., Jiang, W., Rodriguez, F., Rigali, E., Weitzman, S., Porter, V., Rubbi, L.,
Morselli, M., Pellegrini, M., Fiala, M. Omega-3 fatty acids increase the unfolded
protein response and improve amyloid-β phagocytosis by macrophages of patients
with mild cognitive impairmen
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
DNA methylation estimation using methylation-sensitive restriction enzyme bisulfite sequencing (MREBS)
No full textWhole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite
sequencing (RRBS) are widely used for measuring DNA methylation levels on a
genome-wide scale. Both methods have limitations: WGBS is expensive and
prohibitive for most large-scale projects; RRBS only interrogates 6-12% of the
CpGs in the human genome. Here, we introduce methylation-sensitive restriction
enzyme bisulfite sequencing (MREBS) which has the reduced sequencing requirements
of RRBS, but significantly expands the coverage of CpG sites in the genome. We
built a multiple regression model that combines the two features of MREBS: the
bisulfite conversion ratios of single cytosines (as in WGBS and RRBS) as well as
the number of reads that cover each locus (as in MRE-seq). This combined approach
allowed us to estimate differential methylation across 60% of the genome using
read count data alone, and where counts were sufficiently high in both samples
(about 1.5% of the genome), our estimates were significantly improved by the
single CpG conversion information. We show that differential DNA methylation
values based on MREBS data correlate well with those based on WGBS and RRBS. This
newly developed technique combines the sequencing cost of RRBS and DNA
methylation estimates on a portion of the genome similar to WGBS, making it ideal
for large-scale projects of mammalian genome
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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