12,812 research outputs found
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
XVI Congreso de la Sociedad Española de Malherbología: Actas. Pamplona-Iruña, 25-27 octubre, 2017.
No full textComunicaciones presentadas al XVI Congreso de la Sociedad Española de Malherbología, celebrado en la Universidad Pública de Navarra, Pamplona-Iruña, entre los días 25 y 27 de octubre de 2017.Editoras de la obra: Mercedes Royuela Hernando y Ana Zabalza AznárezEste congreso bienal, promovido por la Sociedad Española de Malherbología es la principal reunión científica dedicada al estudio de la Malherbología que se celebra de forma periódica en España.
El Congreso es un foro para dar a conocer los últimos avances conceptuales y tecnológicos en el ámbito de la Malherbología, propiciando, a la vez, el intercambio y la difusión de ideas entre los participantes.
Es imprescindible analizar las diversas estrategias de control de malas hierbas desde una perspectiva amplia, avanzando hacia a un manejo integrado y sostenible. El contexto actual de progresiva reducción en la disponibilidad de materias activas, y de sostenida evolución de poblaciones de malas hierbas resistentes a uno o más herbicidas, no hace sino impulsar aún más este planteamiento. La viabilidad y éxito del manejo implica un trabajo multidisciplinar que incluye el estudio de la biología y ecología de poblaciones, el manejo integrado, el control químico, las resistencias y la eco-innovación.
El Congreso se estructura en diferentes sesiones que abarca los principales ámbitos de la Malherbología y ha contado con la presencia de expertos malherbólogos internacionales de reconocido prestigio en las diferentes temáticas
Estudio de la convergencia regional española
No full textTreballs Finals de Grau de Matemàtiques, Facultat de Matemàtiques, Universitat de Barcelona, Any: 2013, Director: Vicente Royuela MoraIn this thesis the economic and social convergence in Spain since 1988 to 2012 is analysed.
Generally, the convergence analysis among the different Spanish regions is usually carried out by comparing real GPD per capita. However, if we only consider this variable, it just shows a partial view of convergence phenomenon. In fact, this variable doesn’t provide any information about life expectancy or education level. Consequently, the analysis is carried out from the point of view of social well-being in terms of GPD per capita, education level, life expectancy and rate of unemployment. In this research the techniques have been used for finding convergence are beta and sigma convergence.
Another interesting question that arises when different variables in a geographic area are analysed is the spatial autocorrelation. It measures the correlation of a variable with itself through space and I the Moran is statistician used. For example, measurements made at nearby regions may be closer in value than measurements made at regions farther apart. In the same way, this fact is studied remains during the following years. The relationship between convergence and spatial autocorrelation is another important question in regional convergence so it’s analysed.
The structure of this research is as follows. In the first place, the aims of this study and the reasons for doing it are described. Then, a brief description about the economic history context of Spain since 1988 to 2012 is given. Before beginning the convergence study, the process of how all the variables and estimations are obtained is explained. When the database has been built up, the statistic analysis is done. After that, if any autocorrelation exists among regions and whether it remains over time is studied. Finally, an analysis of the beta and sigma convergences with the economic and social variables is carried out
Estudio de la convergencia regional española
No full textTreballs Finals de Grau de Matemàtiques, Facultat de Matemàtiques, Universitat de Barcelona, Any: 2013, Director: Vicente Royuela MoraIn this thesis the economic and social convergence in Spain since 1988 to 2012 is analysed.
Generally, the convergence analysis among the different Spanish regions is usually carried out by comparing real GPD per capita. However, if we only consider this variable, it just shows a partial view of convergence phenomenon. In fact, this variable doesn’t provide any information about life expectancy or education level. Consequently, the analysis is carried out from the point of view of social well-being in terms of GPD per capita, education level, life expectancy and rate of unemployment. In this research the techniques have been used for finding convergence are beta and sigma convergence.
Another interesting question that arises when different variables in a geographic area are analysed is the spatial autocorrelation. It measures the correlation of a variable with itself through space and I the Moran is statistician used. For example, measurements made at nearby regions may be closer in value than measurements made at regions farther apart. In the same way, this fact is studied remains during the following years. The relationship between convergence and spatial autocorrelation is another important question in regional convergence so it’s analysed.
The structure of this research is as follows. In the first place, the aims of this study and the reasons for doing it are described. Then, a brief description about the economic history context of Spain since 1988 to 2012 is given. Before beginning the convergence study, the process of how all the variables and estimations are obtained is explained. When the database has been built up, the statistic analysis is done. After that, if any autocorrelation exists among regions and whether it remains over time is studied. Finally, an analysis of the beta and sigma convergences with the economic and social variables is carried out
Channelrhodopsins: A bioinformatics perspective
No full textAbstractChannelrhodopsins are microbial-type rhodopsins that function as light-gated cation channels. Understanding how the detailed architecture of the protein governs its dynamics and specificity for ions is important, because it has the potential to assist in designing site-directed channelrhodopsin mutants for specific neurobiology applications. Here we use bioinformatics methods to derive accurate alignments of channelrhodopsin sequences, assess the sequence conservation patterns and find conserved motifs in channelrhodopsins, and use homology modeling to construct three-dimensional structural models of channelrhodopsins. The analyses reveal that helices C and D of channelrhodopsins contain Cys, Ser, and Thr groups that can engage in both intra- and inter-helical hydrogen bonds. We propose that these polar groups participate in inter-helical hydrogen-bonding clusters important for the protein conformational dynamics and for the local water interactions. This article is part of a Special Issue entitled: Retinal Proteins — You can teach an old dog new tricks
Representações da sexualidade na obra de Ana Saldanha
No full textMestrado em Línguas, Literaturas e CulturasA presente dissertação analisa as diferentes representações da sexualidade na obra literária para jovens de Ana Saldanha. A leitura analítica incide sobre as narrativas da autora que abordam a temática da sexualidade juvenil e as suas problemáticas, com o objetivo de estudar a forma como as mesmas contribuem para a construção da identidade pessoal e sexual do potencial recetor juvenil. Desta forma, pretende-se detetar os conflitos psicológicos dos protagonistas, os seus valores e problemas, assim como os novos valores, problemas e preocupações sociais que emergem das narrativas; compreender de que forma a autora exprime a sua capacidade em observar o quotidiano e as relações humanas; identificar os pilares em que se apoia o universo social em estudo (família, escola, grupo de amigos); enquadrar as personagens numa sociedade pós-industrial e de informação que exige ao adolescente uma superação de desafios e de novos problemas.This dissertation analyzes the different representations of sexuality in Ana Saldanha’s literary work for youth. The analytical reading focuses on the author's narratives that address the issue of teenage sexuality and its problems, aiming to study their contribution in the construction of personal and sexual identity of the potential juvenile receiver. Thus, it is intended to detect the psychological conflicts of the protagonists, their values and problems, as well as the new values, social concerns and problems that emerge from the narratives; understand how the author expresses her ability to observe daily life and human relations; identify the pillars on which relies the social universe under study (family, school, friends); framing the characters in a post-industrial and information society which requires the teenager to relentlessly surmount challenges and new problems
Viajantes ex-cêntricas nas histórias de Ana Miranda
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones
Séquence 4, Ana et la télévision, raconté par Ana
No full textCette séquence fait partie du corpus vidéo de Langue des Signes Emergentes utilisé lors de la thèse d'Ivani Fusellier-Souza : la locutrice Ana raconte qu'elle aime regarder la télé, bien que ne peut l'écouter. Alors que sa mère ne la regarde pas.00:00:00>00:00:03 La sœur demande à Ana si elle aime bien regarder la télévision. 00:00:04>00:00:07 Ana dit qu'ils ont une télé là-bas. 00:00:07>00:00:15 La sœur demande à nouveau à Ana si elle aime ou non la télé. 00:00:15>00:00:19 La mère dit qu'il faut faire attention, car si on allume la télé, Dieu nous punira. 00:00:19>00:00:48 Ana et sa sœur discute sur le fait qu'Ana ne peut pas écouter la télé et peut seulement la regarder, alors que ses sœurs peuvent l'écouter. 00:00:48>00:01:09 La sœur lui demande ce qu'elle pense des filles à la télé en mini-jupe avec des débardeurs et du rouge à lèvres. 00:01:09>00:01:35 Ana dit qu'elle n'aime pas comme sa sœur les filles qui s'habillent comme cela. 00:01:35>00:01:39 La mère dit que de toute façon, Dieu les punira. 00:01:40>00:01:53 Ana explique qu'elle regarde la télé avec ses sœurs mais que sa mère ne la regarde pas
ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides
No full textHerbicides inhibiting aromatic or branched-chain amino acid biosynthesis trigger similar physiological responses in plants, despite their different mechanism of action. Both types of herbicides are known to activate ethanol fermentation by inducing the expression of fermentative genes; however, the mechanism of such transcriptional regulation has not been investigated so far. In plants exposed to low-oxygen conditions, ethanol fermentation is transcriptionally controlled by the ethylene response factors-VII (ERF-VIIs), whose stability is controlled in an oxygen-dependent manner by the Cys-Arg branch of the N-degron pathway. In this study, we investigated the implication of ERF-VIIs on the regulation of the ethanol fermentation pathway in herbicide-treated Arabidopsis plants grown under aerobic conditions. Our results demonstrate that these transcriptional regulators are stabilized in response to herbicide treatment and required for ethanol fermentation in these conditions. We also observed that mutants with reduced fermentative potential exhibit higher sensitivity to herbicide treatments, thus revealing the existence of a mechanism that mimics oxygen deprivation to activate metabolic pathways that enhance herbicide tolerance. We speculate that this signalling pathway may represent a potential target in agriculture to affect tolerance to herbicides that inhibit amino acid biosynthesis
[YIA] different oxidative pattern in sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting amino acid biosynthesis
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