30,550 research outputs found
The marriage record of Leal, Manuel de la Concepcion and Rodriguez, Eloisa Pells
No full textMarriage license for Manuel de la Concepcion Leal and Eloisa Pells Rodriguez. H.B. Sorneillan was the officiant
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Manuel J. Rodriguez stands next to his portrait of Dr. Garcia. (photograph)
No full textPortrait of Manuel J. Rodriguez of Lomita, California. Mr. Rodriguez is the painter of the painting of Dr. Hector P. Garcia
[Letter from Manuel Rodriguez-Bascur and Roberto Rocha to Manuel Gonzales - June 23, 1977]
No full textLetter from Manuel Rodriguez-Bascur, Secretary, and Roberto Rocha, President, to Manuel Gonzales, LULAC National President, dated June 23, 1977. The letter requests the removal of Joe Velez as League of United Latin American Citizens California State Director
Brief von Juan Manuel Rodriguez an Josef Steindl
No full textBRIEF VON JUAN MANUEL RODRIGUEZ AN JOSEF STEINDL
Brief von Juan Manuel Rodriguez an Josef Steindl ([1]
Angel Manuel Rodriguez
No full textAngel Manuel Rodriguez is director of the Biblical Research Institute at the General Conference of Seventh-day Adventists, where he has served since 1992. He has also served as president of Antillian College and academic vice president of Southwestern Adventist University. Rodriguez holds a ThD degree in biblical theology from Andrews University. He is the author of several books and has a monthly column in Adventist World.https://digitalcommons.andrews.edu/qod-images/1002/thumbnail.jp
Rodriguez, Manuel
No full textCentro Asturiano membership record of Manuel Rodriguez; Socio Number: 41314.https://digitalcommons.usf.edu/asturiano_membership/5005/thumbnail.jp
Gisleno Rodriguez, Manuel
No full textCentro Asturiano membership record of Manuel Gisleno Rodriguez; Socio Number: 32012.https://digitalcommons.usf.edu/asturiano_membership/3024/thumbnail.jp
Garcia Rodriguez, Manuel
No full textCentro Asturiano membership record of Manuel Garcia Rodriguez; Socio Number: 14461.https://digitalcommons.usf.edu/asturiano_membership/3001/thumbnail.jp
Marriage record of Alvarez, Manuel Rodriguez and Rodriguez, Ramona Fernandez
No full textMarriage license for Manuel Rodriguez Alvarez and Ramona Fernandez Rodriguez. J.T. Young was the Justice of the Peace
Experimental data about Antioxidants inhibition during equine sperm capacitation
No full textIn vitro embryo production (IVP) is a highly demanded technique in the animal breeding industry. However, the conventional protocol based on in vitro fertilization (IVF) still shows low success rates in horses. For this reason, intracytoplasmic sperm injection (ICSI) is currently the most widely used method. Unlike in other species where IVF has long been established and yields higher success rates than ICSI, equine sperm capacitation remains a significant challenge. A repeatable IVF protocol was only published in 2022, which requires prolonged sperm capacitation (22 hours at 38 ºC), and not all ejaculates can withstand this process. Given the growing interest in applying IVF to equine reproduction and the known involvement of reactive oxygen species (ROS) in sperm capacitation, this study aimed to enhance sperm capacitation in fresh semen by inhibiting intracellular antioxidant systems, using three specific inhibitors. The study compared the previously described capacitation protocol (CAP) with a non-capacitating control (CTR) and with CAP supplemented with the inhibitors, through four independent experiments:
- Experiment 1: Capacitation in the presence of 3-amino-1,2,4-triazole (3-AT), a catalase inhibitor.
- Experiment 2: Capacitation with LCS-1, an inhibitor of superoxide dismutase 1 (SOD1).
- Experiment 3: Capacitation with L-Buthionine-(S,R)-Sulfoximine (BSO), an inhibitor of
γ-glutamylcysteine synthetase, a key enzyme in glutathione (GSH) synthesis.
- Experiment 4: Assessment of capacitation progression with a combination of the three inhibitors used in the previous experiments.
In experiments 1-3, sperm samples were incubated for 22 hours at 38 ºC and evaluated at 0, 3, and 22 hours. In experiment 4, samples were incubated for 24 hours and analyzed every 3 hours.
The datasets include flow cytometry data on sperm viability, mitochondrial membrane potential, membrane lipid scrambling, intracellular calcium and ROS levels, and protein tyrosine phosphorylation. Additionally, they include kinetic sperm parameters analyzed by computer-assisted sperm analysis (CASA)
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