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Dataset about modulation of mitochondrial bioenergetics by phytochemicals: effect on F1FO-ATPase, mitochondrial respiration and permeability transition pore
No full textThis dataset contains data from the evaluation of the effect of S-allyl cysteine (SAC), Benzyl isothiocyanate (BITC), and Epicatechin (EPC) on mitochondria isolated from porcine hearts. The aim of the research was to verify the modulatory effect of the various molecules on mitochondrial bioenergetics through structure/activity correlation studies of the mitochondrial enzyme F1FO-ATPase, understand the involvement of the redox state on the induced effects, verify the kinetic parameters, and evaluate the effect on the phenomenon of the opening of the mitochondrial permeability transition pore (mPTP), involved in regulated cell death processes. The results, reported in “SAC_BITC_EPC_mitochondria_Dataset.xlsx”, show that it was possible to calculate Hill coefficients (nHi) for the hydrolytic activity of the Mg2+-activated enzyme in the absence and presence of BITC (“nHi_BITC” sheet) and SAC (“nHi_SAC” sheet), and for the Ca2+-activated enzyme in the absence and presence of BITC+ dithiothreitol (DTT) (“nHi_BITC_DTT_Ca_sub; “nHi_BITC_DTT_Ca_cof “ sheets). SAC showed an activating effect on Mg2+-activated F1FO-ATPase (“SAC_MgATPase” sheet) but not when activated by Ca2+ (“SAC_CaATPase” sheet) and its activating effect was lost in the presence of DTT (“SAC_MgATPaseDTT” sheet). BITC showed an inhibit effect on Mg2+-activated F1FO-ATPase (“BITC_MgATPase” sheet) but not when activated by Ca2+ (“BITC_CaATPase” sheet). In presence of DTT, the effect of BITC on Mg2+-activated enzyme was not modulated (“BITC_MgATPaseDTT” sheet) while it was enhanced on Ca2+-activated enzyme (“BITC_CaATPaseDTT” sheet). EPC did not alter Mg2+- (“EPC_MgATPase” sheet) or Ca2+-activated F1FO-ATPase (“EPC_CaATPase” sheet) in any way. SAC showed competitive activation for the cofactor Mg2+ (“Kinetics SAC_cofactorMg” sheet) and uncompetitive activation for the substrate ATP (“Kinetics SAC_substrateATP” sheet). BITC inhibited with a mixed-type mechanism towards the substrate (“Kinetics BITC_substrateATP” sheet) and the cofactor Mg2+ (“Kinetics BITC_cofactorMg” sheet). The inhibition of BITC on Ca2+-activated F1FO-ATPase exerted in the presence of DTT was found to be of mixed type towards the substrate ATP (“Kinetics BITC_substrate_Ca” sheet) and cofactor Ca2+ (“Kinetics BITC_cofactorCa” sheet). SAC and EPC did not alter mitochondrial respiration as assessed by Complex I of the electron transport chain (NADH-O2 oxidoreductase activity) and Complex II (SUCC-O2 oxidoreductase activity) (“SAC NADH-SUCC_O2”; “EPC NADH-SUCC_O2” sheets). BITC also inhibited respiration at high concentrations (“BITC NADH-SUCC_O2” sheet). The modulatory effect of SAC, BITC and EPC was also evaluated on CRC (“CRC” sheet), an index of mPTP opening
Dataset about substitution of Mg2+ cofactor with Ca2+ disrupt positive cooperativity in F1FO-ATP(hydrol)ase catalysis
No full textThis dataset contains data from the evaluation of the hydrolytic activity of mitochondrial F1FO-ATPase, isolated from porcine heart, activated by the natural cofactor Mg²⁺ or replaced by Ca²⁺. The aim of the research was to investigate how replacing the physiological cofactor Mg²⁺ with Ca²⁺ affects the catalysis, cooperativity, and mechanochemical functionality of mitochondrial F1FO-ATPase, in order to understand the molecular mechanisms that determine the transition of the enzyme from its physiological role as an ATP synthase (the "enzyme of life") to its pathological function as a component of the mitochondrial permeability transition pore (mPTP, "enzyme of death"). "F_ATPase_cooperativity_Dataset.zip" contains data on the activity of the Mg2+-dependent F1FO-ATPase, which is more efficient than the Ca2+-dependent activity ("curve_MgATPase.csv" and "curve_CaATPase.csv"). Evaluation of the cooperativity of the Mg²⁺- and Ca²⁺-dependent F1FO-ATPase revealed a differential cooperative effect on the ATP substrate, depending on the specific cofactor involved. A loss of cooperativity is evident when Mg²⁺ ("nHi_MgATPase.csv") is replaced with Ca²⁺ ("nHi_CaATPase.csv"). Data from experiments investigating the kinetics of the cofactors interacting with the enzyme are reported in "Inhibition mechanism.csv", which show a reduction in kinetic parameters in presence of Ca2+, suggesting uncompetitive inhibition
Data about the role of RXRγ in T3-mediated oligodendrocyte precursor cell differentiation
No full textThis dataset contains data describing the effect of the knock-out of the Rxrg gene in neral stem cell (NSC)-derived oligodendrocyte precursor cells (OPCs) differentiation and maturation. Data has been obtained by transcriptomic analysis using RNAseq in cells isolated by WT and Rxrg-/- animals cultured as indifferentiated precursors and 24 hours after the differentiation induction by triiodotironine (T3) exposure, mimiking the physiologic differentiation/maturation molecular machinery.
RNAseq data has been validated using morphological analysis. Molecular tools has been used to inhibit (cyclopamine, GANT61) or activate (SAG, purmorphamine) the sonic hadgehog (SHH) pathway in differentiating WT and Rxrg-/- OPC cultures. Specific markers (NG2, MBP) have been used to quantify the differentiation/maturation of the OPC culture, measuring the percentage of precursors and mature oligodendrocytes
Protective effect of melatonin on hypoxia/reoxygenation-impaired cellular respiration in aortic endothelial cells (pAECs): action on mitochondrial bioenergetics by targeting the F1FO ATPase
Full text linkThis dataset contains data from the evaluation of the effect of melatonin on isolated mitochondria from porcine heart and on a hypoxia/reoxygenation (H/R) model of porcine aortic endothelial cells (pAECs). The aim of the research was to verify the effect of melatonin on mitochondrial bioenergetics by performing structure/activity correlation studies, on its antioxidant effect towards anion superoxide (SOX) production, on the phenomenon of mitochondrial permeability transition pore (mPTP) opening involved in regulated cell death processes and the protective effect on H/R-induced damage towards bioenergetic parameters in an in vitro model on pAECs. The results, reported in “Melatonin_mitochondria_pAECs_Dataset.xlsx”, showed that melatonin has an inhibitory effect on Mg2+-activated F1FO-ATPase at low temperatures (“Different T_Arrhenius_Mg” sheet) and not at 37°C, the physiological temperature. However, in this latter condition melatonin exerts an inhibitory effect on the enzymatic activity of Ca2+-activated F1FO-ATPase (as reported in “Titration curves” sheet), a molecular condition related to mPTP opening. Uncompetitive inhibition (“Inhibition kinetics_Ca” sheet) is exerted by binding to a site in the hydrophilic F1 portion of the enzyme (“F1-ATPase” and “Mutual exclusion_NBD_Ca” sheets). Consistently, mPTP opening was also desensitized by melatonin (“CRC” sheet). Its inhibitory effect was confirmed on the oxidative phosphorylation (“OXPHOS Site I” and “OXPHOS Site II” sheets) process when mitochondria were energized from the first phosphorylation site (Complex I). Its antioxidant effect was confirmed on SOX production (“ROS_Site I” and “ROS_Site II” sheets), in isolated mitochondria, induced from phosphorylation site I (Complex I) and site II (Complex II). pAEC viability was impaired by melatonin starting at a dose of 5 mM. After hypoxia/reoxygenation (H/R) treatment, cells appeared detached and phenotypically modified and the presence of 1 mM melatonin during H/R restored the adherent monolayer and cell viability was not different from normoxia control cells (“pAEC viability” sheet). The damage induced by the H/R process is reflected in mitochondrial bioenergetic parameters and increased SOX production; melatonin exerts a protective effect, improving bioenergetic parameters and reducing SOX levels (“Metab_ROS pAEC_H_R” sheet)
Florentia Illustrata Knowledge Graph
No full textThis dataset is part of the master's thesis project "Florentia Illustrata", developed in collaboration with I Tatti - The Harvard University Center for Italian Renaissance Studies in Florence. The project was conducted during an internship for thesis purposes within the Master's Degree in Digital Humanities and Digital Knowledge at the University of Bologna, with a focus on Knowledge Management. The "Florentia Illustrata Knowledge Graph" is a structured and semantically enriched digital representation of Florence in the first half of the 19th century. It was developed from the historical study by Belli, Lucchesi, and Raggi (2022): Firenze nella prima metà dell'Ottocento: La città nei documenti del Catasto Generale Toscano (Firenze University Press, DOI: 10.36253/979-12-215-0002-8).
The dataset captures historical, geographical, and social aspects of the city, mapping places, people, events, and their interconnections and relate them in a knowledge graph structured according to CIDOC CRM standards. It can support historical research, cultural heritage studies, and digital humanities applications
L’Indicatore Correttivo di Onerosità Territoriale (ICOT) dell'Assistenza Domiciliare Integrata (ADI) per interpretare la performance e modulare il finanziamento nei diversi territori
No full textL'indicatore Correttivo di Onerosità Territoriale dell'Assistenza Domiciliare (ICOT) permette di esprimere il peso delle diverse conformazioni abitative e demografiche dei territori sulle risorse impiegate nell'Assistenza Domiciliare Integrata superando i vincoli relativi alla riservatezza e consentendo di adeguare alle condizioni del territorio e della popolazione le valutazioni relative all'efficienza
The Loginova Russian NGOs Cooptation Dataset 2023 (LOruNCD)
No full textThis dataset aims to quantitatively assess both formal and informal co-optation strategies targeting environmental non-governmental organizations (NGOs) in Russia during the year 2023. It is theoretically grounded in the typology of government-organized NGOs (GONGOs) proposed by Hasmath and Hsu (2019), which examines the origins of NGOs and their institutional ties to the state. Building upon this foundational framework, the dataset expands the analytical scope to incorporate a wider array of stakeholders, including public enterprises, private businesses, and foreign actors. The current version of the dataset encompasses 75 variables across a sample of 41 environmental NGOs that participated in major government-sponsored forums in 2023. This approach enables a detailed examination of the degree and nature of co-optation experienced by each organization through interactions with various institutional actors. The dataset is organized into six thematic sections: (1) general information, (2) institutional constraints, (3) foreign influence, (4) ties with the government, (5) ties with public enterprises, and (6) ties with private businesses. Variable construction draws on established academic literature on the shrinking space for civil society and the mechanisms of NGO co-optation in authoritarian regimes. All data were compiled using open-source materials, including content made publicly available by NGOs on their official websites. In the case of GONGOs, additional information was sourced from news media, legal filings submitted to Russian tax authorities, annual reports, and other relevant documents. In total, the dataset is informed by over 4,000 individual sources. Given the constraints of operating in an authoritarian context, access to comprehensive information is often limited. Therefore, a value of “0” for any variable signifies the absence of publicly available evidence supporting the associated assumption, rather than a definitive absence of the phenomenon itself.
This dataset is designed to support a range of scholarly inquiries, including analyses of co-optation dynamics in authoritarian regimes, patterns of foreign and domestic partnerships among NGOs and GONGOs, variations in state-civil society relations, and the participation of NGOs in state-aligned or propagandistic initiatives. It offers a unique empirical foundation for understanding the evolving strategies of stakeholder-led co-optation in Russia and may be adapted for comparative studies in other authoritarian settings
WRITE. New Forms of Calligraphy in Contemporary China. Dataset
No full textThe current dataset is associated with the Open Access publication “New Forms of Calligraphy in Contemporary China” (KERVAN - International Journal of African and Asiatic Studies, vol. 28 No. 2 2024, DOI https://doi.org/10.13135/1825-263X/11615). It collects the metadata descriptions of the main artworks analysed in the publication as formalised in the “WRITE digital archive”, that is the semantic web digital archive of the WRITE project (G.A. 949645). Each file contains the metadata related not only to the artwork(s) itself, but also to its author(s), Calli-Writing Unit(s) (CWU – artwork content unit with calligraphic features), and, if any, related exhibition(s), artwork(s), and literary work(s).The artworks were selected from a large number of works included in the archive as the most representative of the new forms of calligraphy emerged in contemporary China
Affettività e Diritto. La risorsa dell’intimità, Jullien lettore di Simenon
Full text link[Affectivity and Law. The resource of intimacy, Jullien reader of Simenon] This paper aims to analyze a particular dimension of being human: intimacy. To this end, the reading that François Jullien offers, starting by discussing a few pages of the work, Simenon's Train, is useful. The idea is to think of a juridicity that is no longer closed in its schemes but open and that finds a common space of sharing in respect for the other; this mechanism is, in Jullien discerned in intimacy that in its structure provides for two subjects to meet in a defined space of thirdness that in law is the space of juridicity that turns ( following Heritier) toward an affective turn
The Impact of Within-Occupation Technological Change on Spatial Sorting and Wage Inequality
Full text linkBoth the demand for skilled labor and the skill wage premium have become increasingly dispersed across the United States. This paper examines how technological change within occupations drives these uneven local developments. Combining a novel measure of technological change—capturing shifts in task intensities within 430 detailed occupations—with patent data and microdata, I demonstrate that innovation reallocates labor toward cognitive-intensive tasks, especially in densely populated areas. Motivated by this, I show that greater exposure to technological change increases the relative employment of college-educated workers while causing within-occupation wage declines for less-educated workers, widening the college wage premium