7 research outputs found
The need for transparency and good practices in the qPCR literature.
No full textTwo surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not
Tres temazcales mayas: los casos de Acanmul, Edzná y Oxkintok. 50. Arqueología
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Implementation of Information Security Audit for the Sales System in a Peruvian Company
Get PDFTechnology has been updated over the last few years, and this has been generating a worldwide impact as currently, in this pandemic, several companies have been victims of information theft through hacks, as some companies do not have audits so that they can protect their information. The management of computer security audits in companies is very important to detect possible risks and manage business control by applying continuity management in each disaster. The article's main objective is to implement an audit plan and information security through ISO 27001 for a sales system to improve computer security. The literature review is on the definition of several processes that are part of our implementation development. Our methodology employed five stages of project management (Start, Planning, Execution, Monitoring and control, and closure), explaining the procedure and definition of each stage. The case study is the development of each stage that identifies the risks and obtains a solution to any threat. The results are the treatments of the risks carried out in the company, explaining the compliance with the clause and controls of ISO 27001 in the company. Finally, the analysis of the indicators of each policy of the company to know the improvement the company Domingez
Society and the inquisition in Malta 1743-1798
Get PDFThe thesis falls into two main sections. It endeavours to analyse the major aspects of Maltese society in the second half of the eighteenth century as they emerge from a close scrutiny of the Archives of the Inquisition in Malta. The approach is mainly that of the ethnologist, a social history written 'from below'. The ultimate purpose was to try to arrive at as clear and accurate a picture of the Maltese mentalite as the archives permitted. Unfortunately, the Archives of the Inquisition in Malta have hardly ever been seriously studied by the social historian. Their richness and diversity not only cast enormous insight into the mental habits and frame of mind of a wide cross-section of Maltese society; they even shed sufficient light on a wide range of the social life of the Maltese. The subject is also approached from the point of view of the legal historian. The Inquisition was a Tribunal of Faith set up to stop the onslaught of Protestantism, as well as to reform the superstitious accretions to popular religion practised by the remaining part of the Catholic Church. The thesis examines the events leading to the charge and possibly arrest of the accused. Most of the reports were self-accusations and those arrested were taken into custody only after much deliberation. If the Inquisitors did make use of torture the accused was assisted by the defence counsel and produced his own witnesses. No instance of death sentences are encountered with in the second half of the eighteenth century and those found guilty were kindly dealt with, the Inquisitors being only after their conversion
Understanding the functionality of transcript diversity
Get PDFRecent years have seen a huge increase in the amount of genomic DNA
being sequenced from a wide variety of organisms, giving us an unprecedented
insight into the molecular diversity seen in nature. As a
result a host of methods have been developed, both experimental and
computational, to understand the functional significance of such diversity
and how it relates to organismal and environmental complexity.
In this thesis I use comparative approaches to explore two areas of
molecular biology where there is evidence for large amounts of transcript
diversity. Firstly, I explore the unprecedented view of microbial
sequence diversity offered by metagenomic sequencing projects, using
sequence similarity and adapted genomic context methods to quantify
the amount of functional novelty in these samples. Secondly, I look
at the transcript diversity generated by alternative splicing. I develop
methods to detect and visualise alternative splicing events and apply
these to the detection of conserved alternative splicing events
Efficacy, safety, and immunogenicity of a booster regimen of Ad26.COV2.S vaccine against COVID-19 (ENSEMBLE2) : results of a randomised, double-blind, placebo-controlled, phase 3 trial
No full textBackground Despite the availability of effective vaccines against COVID-19, booster vaccinations are needed to maintain vaccine-induced protection against variant strains and breakthrough infections. This study aimed to investigate the efficacy, safety, and immunogenicity of the Ad26.COV2.S vaccine (Janssen) as primary vaccination plus a booster dose.
Methods ENSEMBLE2 is a randomised, double-blind, placebo-controlled, phase 3 trial including crossover vaccination after emergency authorisation of COVID-19 vaccines. Adults aged at least 18 years without previous COVID-19 vaccination at public and private medical practices and hospitals in Belgium, Brazil, Colombia, France, Germany, the Philippines, South Africa, Spain, the UK, and the USA were randomly assigned 1:1 via a computer algorithm to receive intramuscularly administered Ad26.COV2.S as a primary dose plus a booster dose at 2 months or two placebo injections 2 months apart. The primary endpoint was vaccine efficacy against the first occurrence of molecularly confirmed moderate to severe-critical COVID-19 with onset at least 14 days after booster vaccination, which was assessed in participants who received two doses of vaccine or placebo, were negative for SARS-CoV-2 by PCR at baseline and on serology at baseline and day 71, had no major protocol deviations, and were at risk of COVID-19 (ie, had no PCR-positive result or discontinued the study before day 71). Safety was assessed in all participants; reactogenicity, in terms of solicited local and systemic adverse events, was assessed as a secondary endpoint in a safety subset (approximately 6000 randomly selected participants). The trial is registered with ClinicalTrials.gov, NCT04614948, and is ongoing.
Findings Enrolment began on Nov 16, 2020, and the primary analysis data cutoff was June 25, 2021. From 34 571 participants screened, the double-blind phase enrolled 31 300 participants, 14 492 of whom received two doses (7484 in the Ad26.COV2.S group and 7008 in the placebo group) and 11 639 of whom were eligible for inclusion in the assessment of the primary endpoint (6024 in the Ad26.COV2.S group and 5615 in the placebo group). The median (IQR) follow-up post-booster vaccination was 36 center dot 0 (15 center dot 0-62 center dot 0) days. Vaccine efficacy was 75 center dot 2% (adjusted 95% CI 54 center dot 6-87 center dot 3) against moderate to severe-critical COVID-19 (14 cases in the Ad26.COV2.S group and 52 cases in the placebo group). Most cases were due to the variants alpha (B.1.1.7) and mu (B.1.621); endpoints for the primary analysis accrued from Nov 16, 2020, to June 25, 2021, before the global dominance of delta (B.1.617.2) or omicron (B.1.1.529). The booster vaccine exhibited an acceptable safety profile. The overall frequencies of solicited local and systemic adverse events (evaluated in the safety subset, n=6067) were higher among vaccine recipients than placebo recipients after the primary and booster doses. The frequency of solicited adverse events in the Ad26.COV2.S group were similar following the primary and booster vaccinations (local adverse events, 1676 [55 center dot 6%] of 3015 vs 896 [57 center dot 5%] of 1559, respectively; systemic adverse events, 1764 [58 center dot 5%] of 3015 vs 821 [52 center dot 7%] of 1559, respectively). Solicited adverse events were transient and mostly grade 1-2 in severity.
Interpretation A homologous Ad26.COV2.S booster administered 2 months after primary single-dose vaccination in adults had an acceptable safety profile and was efficacious against moderate to severe-critical COVID-19. Studies assessing efficacy against newer variants and with longer follow-up are needed. Funding Janssen Research & Development.
Copyright (c) 2022 The Author(s). Published by Elsevier Ltd
Artes de canto (1492-1626) y mujeres en la cultura musical del mundo ibérico renacentista
Get PDF[spa] Esta Tesis Doctoral explora la cultura musical del mundo ibérico renacentista a través del estudio de artes de canto en lengua vernácula impresas entre 1492 y 1626 y de su relación con las mujeres de la época. La Tesis consta de siete capítulos, estructurados en dos partes (Vol. I), y de veinte apéndices (Vol. II). La primera parte (Capítulos I al IV) muestra que las características por las que estos libros que contenían los rudimentos de la música han sido infravalorados hasta ahora por la historiografía musical son precisamente las mismas que demuestran que respondían a una necesidad pedagógica y a la demanda de manuales asequibles, breves y prácticos por parte de un amplio mercado. Las artes de canto se imprimieron en tiradas de miles de ejemplares y eran vendidas a bajo precio, tuvieron una enorme circulación en la Península Ibérica y el Nuevo Mundo, y contribuyeron al incremento y la difusión de la educación musical en contextos educativos diferenciados (la iglesia, la universidad y el ámbito privado), así como entre grupos sociales hasta entonces excluidos del aprendizaje de los fundamentos de la música. El Arte de canto llano (Sevilla, 1530) de Juan Martínez emerge como el tratado de música del mundo hispánico más difundido geográfica y cronológicamente en el siglo XVI e inicios del XVII, pero del que casi nada se sabía. En la segunda parte (Capítulos V al VII), las conexiones entre estos libros de música y mujeres muestran que las áreas de superposición entre lo privado y lo público y entre lo oral y lo escrito permiten desafiar la invisibilidad de las mujeres en documentos históricos y vislumbrar trazas no sólo de la cultura musical de las mujeres de la época, sino también de la importancia de la música en la vida cotidiana. Se utiliza una diversidad de fuentes (artes de canto, libros de conducta, documentos inquisitoriales, literatura, correspondencia e inventarios de bienes, entre otras), a través de las cuales se ha podido documentar la relación con la música de, entre otras, Catalina de Zúñiga, VI Condesa de Lemos, Isabel de Plazaola, e Isabel de Aragón, IV Duquesa del Infantado. Empleando metodologías de la musicología tradicional junto a otras tomadas de los historiadores del libro y de la cultura popular, esta Tesis Doctoral presenta una panorámica de la vida musical de la época a través del prisma de doble alteridad que supone el estudio de las artes de canto, generalmente consideradas carentes de interés, y de su relación con las mujeres, insuficientemente representadas en la historiografía musical.[eng] This dissertation explores the musical culture of the Renaissance Iberian world through both the study of small-format treatises in the vernacular containing the rudiments of music –known as artes de canto– printed between 1492 and 1626, and the nexuses between them and women. The dissertation consists of seven chapters, structured into two parts (Volume I), and twenty appendixes (Volume II). Part I (Chapters I to IV) shows that the arte de canto, until now generally overlooked or undervalued in music historiography, was produced in print runs of thousands of copies and sold for a low price; it had a broad circulation in the Iberian Peninsula and the New World, contributing to the spread of musical literacy in distinct didactic contexts (churches, universities, private settings) and among social groups until then excluded from learning the rudiments of music. The little known Juan Martínez’s Arte de canto llano (Seville, 1530) emerges as the most circulated music book in the Hispanic world during the sixteenth and seventeenth centuries. The aim of Part II (Chapters V to VII) is to approach the musical life of the sixteenth-century Iberian world through the exploration of women’s contributions, thus broadening the field of historical research. The study of some connections between music books and women shows that the points of overlap between the private and the public spheres, on the one hand, and the written and the oral music transmission, on the other hand, not only make women’s musical practices visible, but also offer new vistas on the popular culture of the age. Through a variety of source materials (artes de canto, conduct manuals, Inquisition records, literature, letters and inventories of goods, among others) it has been possible to document the musical activities of women such as Catalina de Zúñiga, VI Countess of Lemos, Isabel de Plazaola, and Isabel de Aragón, IV Duchess of the Infantado. Combining methodologies from traditional musicology with those borrowed from book history and popular culture, this dissertation analyzes music in the culture of the Renaissance Iberian world through the prism of double Otherness involved in studying the ‘other’ music books –that is the undervalued artes de canto– and their connections to women of that period
