1,721,102 research outputs found
Proteomic approaches by SELDI and MALDI-TOF/MS for CTL analysis
No full textExtracellular stimuli activate, on target cells, a number of signal transduction processes regulating gene expression and the function and/or synthesis of the proteins. In order to highlight the slight changes of the quantity and quality of the proteome it is essential to optimize preparative strategies able to improve the signals of the less expressed proteins and to standardize the use of high-throughput techniques useful to detect them. We describe a complete workflow useful to enrich, from PBMC protein extracts and extrapolated to their subpopulations, the low-molecular-weight proteins and peptides and to detect them by SELDI-TOF protein profiling. The described protocol can also be applied to MALDI-TOF/MS instruments in order to obtain fast, reproducible, and high-quality protein profiles
Apigenin: Molecular Mechanisms and Therapeutic Potential against Cancer Spreading
Full text linkDue to its propensity to metastasize, cancer remains one of the leading causes of death worldwide. Thanks in part to their intrinsic low cytotoxicity, the effects of the flavonoid family in the prevention and treatment of various human cancers, both in vitro and in vivo, have received increasing attention in recent years. It is well documented that Apigenin (4 ',5,7-trihydroxyflavone), among other flavonoids, is able to modulate key signaling molecules involved in the initiation of cancer cell proliferation, invasion, and metastasis, including JAK/STAT, PI3K/Akt/mTOR, MAPK/ERK, NF-kappa B, and Wnt/beta-catenin pathways, as well as the oncogenic non-coding RNA network. Based on these premises, the aim of this review is to emphasize some of the key events through which Apigenin suppresses cancer proliferation, focusing specifically on its ability to target key molecular pathways involved in angiogenesis, epithelial-to-mesenchymal transition (EMT), maintenance of cancer stem cells (CSCs), cell cycle arrest, and cancer cell death
Novel syntheses of 5-acetyl-2,3-dihydro-1,4-thiazine, a very intense roasty, popcorn-like odorant.
No full textMateriale laminato ad elevato effetto barriera all'ossigeno
No full textUno dei maggiori problemi delle plastiche usate nel settore dell’imballaggio è la loro relativamente scarsa barriera ai gas ed in particolare all’ossigeno. Lo scopo di questo brevetto è la messa a punto di un coating ibrido organico-inorganico a base di PVA e alcossidi di silicio. Questi due composti in soluzione acquosa in ambiente leggermente acido formano una lacca che può essere depositata a spruzzo o mediante stampa su substrati plastici. In alcuni casi (PE, PP) la superficie deve essere attivata mediante effetto corona. La soluzione depositata mediante un leggero riscaldamento dà origine ad un film solido, ma molto elastico che migliora di molto le proprietà barriera. Lo spessore del film dopo trattamento termico è inferiore ad 1 micron. D esempio, la barriera all’ossigeno del PET non ricoperto passa da 30-90 cc/(m2*day) a 0,05-0,5 cc/(m2*day) quando ricoperto con il coating, migliore o uguale ai multistrato contenenti EVOH comunemente utilizzati. Rispetto a questi ultimi materiali i film su cui è stato depositato il coating in oggetto hanno un peso inferiore, quindi parità di superficie trattata si ha un peso inferiore, con risparmio di materiale plastico e conseguentemente con un beneficio ambientale. Inoltre il coating depositato può essere sottoposto a processi di stampa oppure di incollaggio senza alcun problema. La velocità di deposizione su bobine è superiore a 200 m/min
A PK/PD model for tumor-in-host growth kinetics following administration of an antiangiogenic agent given alone or in combination regimens
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Deprotonation of 3-Chloropropenyl-2-Oxazolines: Regio- and Stereochemistry of Reactions with Electrophiles
No full textMechanisms of ß-adrenergic modulation of IKs in the guinea-pig ventricle: insights from experimental and model-based analysis
No full textDetailed understanding of IKs gating complexity may provide clues regarding the mechanisms of repolarization
instability and the resulting arrhythmias. We developed and tested a kinetic model to interpret physiologically relevant IKs properties,
including pause-dependence and modulation by b-adrenergic receptors (b-AR). IKs gating was evaluated in guinea-pig
ventricular myocytes at 36C in control and during b-AR stimulation (0.1 mmol/L isoprenaline (ISO)). We tested voltage dependence
of steady-state conductance (Gss), voltage dependence of activation and deactivation time constants (tact, tdeact), and
pause-dependence of tact during repetitive activations (treact). The IKs model was developed from the Silva and Rudy formulation.
Parameters were optimized on control and ISO experimental data, respectively. ISO strongly increased Gss and its voltage
dependence, changed the voltage dependence of tact and tdeact, and modified the pause-dependence of treact. A single set of
model parameters reproduced all experimental data in control. Modification of only three transition rates led to a second set
of parameters suitable to fit all ISO data. Channel unitary conductance and density were unchanged in the model, thus implying
increased open probability as the mechanism of ISO-induced Gss enhancement. The new IKs model was applied to analyze ISO
effect on repolarization rate-dependence. IKs kinetics and its b-AR modulation were entirely reproduced by a single Markov chain
of transitions (for each channel monomer). Model-based analysis suggests that complete opening of IKs channels within a physiological
range of potentials requires concomitant b-AR stimulation. Transient redistribution of state occupancy, in addition to
direct modulation of transition rates, may underlie b-AR modulation of IKs time dependence
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