1,236 research outputs found
A Secondary Metabolism Inducer of Saccharopolyspora erythraea ATCC 11635
No full textAbstract
A research has been carried out to investigate the time production and the chemical structure of a secondary metabolism inducer produced by Saccharopolyspora erythraea ATCC 11635. The inducer was found out to be produced around time which indicated a kind of "peak" of DO. The structural elucidation analysis showed that the inducer was a tetrahydrofuran derivate. In addition was a guanosine derivative, which was assumed to be ppGpp that is commonly present in a stresses condition.
Keywords: secondary metabolism inducer, Saccharopolyspora erythraea ATCC 1163
Considerations on the electromagnetic flow in Airy beams based on the Gouy phase
No full textWe reexamine the Gouy phase in ballistic Airy beams (AiBs). A physical interpretation of our analysis is derived in terms of the local phase velocity and the Poynting vector streamlines. Recent experiments employing AiBs are consistent with our results. We provide an approach which potentially applies to any finite-energy paraxial wave field that lacks a beam axis.This research was funded by the Spanish Ministry of Economy and Competitiveness under the project TEC2009-11635
A Secondary Metabolism Inducer of Saccharopolyspora erythraea ATCC 11635
No full textAbstract
A research has been carried out to investigate the time production and the chemical structure of a secondary metabolism inducer produced by Saccharopolyspora erythraea ATCC 11635. The inducer was found out to be produced around time which indicated a kind of "peak" of DO. The structural elucidation analysis showed that the inducer was a tetrahydrofuran derivate. In addition was a guanosine derivative, which was assumed to be ppGpp that is commonly present in a stresses condition.
Keywords: secondary metabolism inducer, Saccharopolyspora erythraea ATCC 1163
Diffraction-managed superlensing using metallodielectric heterostructures
No full textWe show that subwavelength diffracted wave fields may be managed inside multilayered plasmonic devices to achieve ultra-resolving lensing. For that purpose we first transform both homogeneous waves and a broad band of evanescent waves into propagating Bloch modes by means of a metal/dielectric (MD) superlattice. Beam spreading is subsequently compensated by means of negative refraction in a plasmon-induced anisotropic effective-medium that is cemented behind. A precise design of the superlens doublet may lead to nearly aberration-free images with subwavelength resolution in spite of using optical paths longer than a wavelength.This research was funded by the Spanish Ministry of Economy and Competitiveness under the project TEC2009-11635
Produksi-Anhidroeritromisin-A Dari Biakan Saccharopolyspora Erythraea ATCC 11635
No full textErythromycin has been used widely to prevent infection diseases which caused by Staphylococcus. However erythromycin is unstable and decomposed in an acid condition. This unstability erythromycin is conducted by due to a nucleophylic attack of the C6-hydroxyl group of erythromycin to its Cg-carbonyl group. This Decomposition can be avoided by modification the erythromycin structuresuch as omitting the C6-hydroxyl group. Biomodification for omitting the C6-hydroxyl group can be conducted by inhibition the activity of enoyl reductase in fourth step of the biosynthesis 6deoxyerythronolid-B. The inhibition process could carried out by an addition of an antimetabolite isonicotonic hidrazide (INH) into the fermentation of erythromycin production. By the enoyl reductase inhibition, the microbe will produce A6.7-Anhydroerythromycin-A which is more stable in an acid condition than erythromycin-A.
This research is to produce derivative A6.7 -Anhydroerythromycin-A by addition of INH into a culture of Saccharopolyspora erythraea ATCC 11635 in medium basal and Hutchinson. Selection of medium for fermentation of Sac. erythraea ATCC 11635 for A6.7-Anhydroerythromycin-A production was done out of two media: basal medium with palm oil and Hutchinson medium. The two media were treated with an additional 0,2% INH as an antimetabolite. Hutchinson medium yielded the highest product of A6.7-Anhydroerythromycin-A.
The FT-IR spectrometric analyzes of metabolite showed a stretching vibration of C=C conjugated group at wave number 1602,7 cm-l. This C=C
conju~ated vibration indicated the existence of double bond between C6 and
C7 (A .7), this confirmed that isolate-C contained A6.7-Anhydroerythromycin-A (the possibility of A6.7 was possitive).
Key words: enoyl reductase, L6,7-anhydroerythromycin-A, isoniazid (INH
Pemanfaatan bekatul untuk meningkatkan produksi eritromisin dari biakan Saccharopolyspora erythraea ATCC 11635 = The Utilization of Rice Bran to Increase the Production of Erythromycin by Saccharopolyspora erythraea ATCC
No full textThe research on the utilization of rice bran to increase the production of erythromycin by Saccharopolyspora erythraea ATCC 11635, had been conducted. The fermentation of Sac. erythraea ATCC 11635 for erythromycin production was done under laboratory scale using media with and without rice bran addition. Rice bran was added with in two forms, the powder and the extract. Two different extracts of rice bran were made using aquadest at room and 40-500C temperatures.
This research was conducted in three phases. The first phase was carrying out fermentations using media with 0, 20, 25, 30, 35 and 40 % additional rice bran powder. The second phase conducty fermentation 1 and 5% additional rice bran powder and extracts. The third phase was done to determine the optimum concentration from the 1-5% additional rice bran extract. As the comparison, the fermentation of Sac. erythraea ATCC 11635 in 1% TSB was also conducted. During the fermentation processes, monitoring was done on the control and each treatment cultures by determining (a) the dry cells weight of the microbe, (b) bioactivitys of erythromycin against Micrococcus luteus ATCC 9341. The bioactivitys were based on the production of erythromycin in each milligram dry cells weight microbe. The main components of the rice bran extract were determined, i. e. sucrose and glucose (Luff Schoorl method), protein (Kjeldahl method), amino acid (HPLC method) and protein molecular weight of the rice bran (Electroforesis method). The result were compared with those from TSB 1% media.
It was showed that the growth occured in media containing 20-40% rice bran with range time from 30-37 days. The additional rice bran powder into the media increased the growth of Sac. erythraea ATCC 11635, while rice bran extract increase not only the growth, but also the production of erythromycin. 3%, room temperature-rice bran extract was the optimum media for erythromycin production and could be increased to 780% than control and 260% than TSB. It can be concluded that rice bran extract could be used as the production media of erythromycin product for Sac. erythraea ATCC 11635.
Key Words : rice bran, production of erythromycin, Saccharopolyspora erythraea ATCC 11635
Biosintesis A6'7-AnhidroeritromisinMelalui Penghambatan Reduksi Enoil Oleh Isoniazid Pada FERMENTASI Saccharopolyspora erythraea ATCC 11635
No full textABSTRACT
The biosynthesis of new erythromycin derivative has been carried out by adding of isoniazid (INH) into fermentation of Saccharopolyspora erythraea ATCC 11635. INH was added to inhibit the enoyl-reduction process in the fourth step of 6- deoxyerythronolid B (6-DEB) biosynthesis, so in the last process was expected to produce Av-anhydroerythromycin which more stable in acidic condition than erythromycin. The isolating new metabolites: A-KG was obtained from the shake fermentation with additional IN
Pemanfaatan bekatul untuk meningkatkan produksi eritromisin dari biakan Saccharopolyspora erythraea ATCC 11635 = The Utilization of Rice Bran to Increase the Production of Erythromycin by Saccharopolyspora erythraea ATCC
No full textThe research on the utilization of rice bran to increase the production of erythromycin by Saccharopolyspora erythraea ATCC 11635, had been conducted. The fermentation of Sac. erythraea ATCC 11635 for erythromycin production was done under laboratory scale using media with and without rice bran addition. Rice bran was added with in two forms, the powder and the extract. Two different extracts of rice bran were made using aquadest at room and 40-500C temperatures.
This research was conducted in three phases. The first phase was carrying out fermentations using media with 0, 20, 25, 30, 35 and 40 % additional rice bran powder. The second phase conducty fermentation 1 and 5% additional rice bran powder and extracts. The third phase was done to determine the optimum concentration from the 1-5% additional rice bran extract. As the comparison, the fermentation of Sac. erythraea ATCC 11635 in 1% TSB was also conducted. During the fermentation processes, monitoring was done on the control and each treatment cultures by determining (a) the dry cells weight of the microbe, (b) bioactivitys of erythromycin against Micrococcus luteus ATCC 9341. The bioactivitys were based on the production of erythromycin in each milligram dry cells weight microbe. The main components of the rice bran extract were determined, i. e. sucrose and glucose (Luff Schoorl method), protein (Kjeldahl method), amino acid (HPLC method) and protein molecular weight of the rice bran (Electroforesis method). The result were compared with those from TSB 1% media.
It was showed that the growth occured in media containing 20-40% rice bran with range time from 30-37 days. The additional rice bran powder into the media increased the growth of Sac. erythraea ATCC 11635, while rice bran extract increase not only the growth, but also the production of erythromycin. 3%, room temperature-rice bran extract was the optimum media for erythromycin production and could be increased to 780% than control and 260% than TSB. It can be concluded that rice bran extract could be used as the production media of erythromycin product for Sac. erythraea ATCC 11635.
Key Words : rice bran, production of erythromycin, Saccharopolyspora erythraea ATCC 11635
Biosintesis A6\u277-AnhidroeritromisinMelalui Penghambatan Reduksi Enoil Oleh Isoniazid Pada FERMENTASI Saccharopolyspora erythraea ATCC 11635
No full textABSTRACT
The biosynthesis of new erythromycin derivative has been carried out by adding of isoniazid (INH) into fermentation of Saccharopolyspora erythraea ATCC 11635. INH was added to inhibit the enoyl-reduction process in the fourth step of 6- deoxyerythronolid B (6-DEB) biosynthesis, so in the last process was expected to produce Av-anhydroerythromycin which more stable in acidic condition than erythromycin. The isolating new metabolites: A-KG was obtained from the shake fermentation with additional INHwhile B-F and D-F were from the fermentor with additional INH. The optimum concentration of additional INH was 0,2%. Based on the FT-IR spectrograms analysis, it was indicated that the B-F and D-F were erythromycin derivatives which have a C=C bond at 06,2 position. This bond is possible to form a conjugation system which result in an enol-form of C=0 at C-9. These new metabolite kept on having acid stability and antibiotic activity in lower pH up to 3which were much better than those of erythromycin A.
Key words: isoniazid, enoyl reduction, Sacchapolyspora erythraea
ATCC 11635, d6,7-anhydroerythromyci
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