51 research outputs found
EFFECTOF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL
No full textLR-2(4-phenyl-3,4-dihydro-indeno[2\u27,1 \u27]pyramidine-2( 1H)- thioneLeni Ritmaleni 2), which designed and assumed to have biologically activity as anticancer, has been successfully synthesized by using the Biginelli reaction. This research was aimed to investigate the effect of benzaldehyde excess in the synthesis of LR-2 and to evaluate the cytotoxic activity of LR-2 against HeLacancercell lines.The synthesis was done by reacting benzaldehyde, 2-indanone and together with thiourea at one time as said as one pot reaction synthetic methodology and the reaction was acid catalysed. The mole equivalent of benzaldehyde was in excess compare to others. The effect of benzaldehyde in excess is the higher the mole of benzaldehyde, the lower the yield of LR-2. The cytotoxicity of LR-2was done by using MTTmethod and the LC₅₀was 268.15 µM
Synthesis of 4 Phenyl 3,4 Dihydr Indeno[2′,1′]Pyrimidine 2 One on Different Amount of Catalysts
No full textDihydropirimidine is a heterocyclic compound which has important pharmacologic and therapeutic activities. This compound can be synthesised by using Biginelli reaction which involve three type of starting materials and also have been developed into different method of reaction condition and starting material. LR1 (4phenyl3,4dihydroindeno[2′,1′]pyrimidine2one) is a derivative of dihydropirimidine. This research was aimed to study the effect of catalyst amount in the synthesis of LR1 in order to reach the optimal yield. LR1 can be synthesised form benzaldehyde, urea adn 2indanone in ethanol and concentrated chloric acid as catalyst for five hours under reflux. The product mixture was then extracted and isolated by column chromatography preparative. The melting point was checked to determine the purity of the product. The structure elucidation was carried out by using spectroscopic method (uvvis, IR, 1HNMR and MS) The result showed that the highest yield of LR1 was obtained around 15 % when 0.25 % of chloric acid used as catalyst. The higher the condition of acid in the reaction, the lower the tield of LR1 obtained
OBAT BATUK GLISERIL GUAIAKOL DARI MINYAK JELANTAH: SEBAGAI MODEL DALAM PEMANFAATAN GLISEROL DARI HASIL SAMPING PRODUKSI BIODIESEL UNTUK SEDIAAN FARMASI
No full textApplication of spiro-epoxides in the synthesis of biologically promising targets
No full textEThOS - Electronic Theses Online ServiceGBUnited Kingdo
Synthesis of 4 Phenyl 3,4 Dihydr Indeno[2′,1′]Pyrimidine 2 One on Different Amount of Catalysts
Full text linkDihydropirimidine is a heterocyclic compound which has important pharmacologic and therapeutic activities. This compound can be synthesised by using Biginelli reaction which involve three type of starting materials and also have been developed into different method of reaction condition and starting material. LR1 (4phenyl3,4dihydroindeno[2′,1′]pyrimidine2one) is a derivative of dihydropirimidine. This research was aimed to study the effect of catalyst amount in the synthesis of LR1 in order to reach the optimal yield. LR1 can be synthesised form benzaldehyde, urea adn 2indanone in ethanol and concentrated chloric acid as catalyst for five hours under reflux. The product mixture was then extracted and isolated by column chromatography preparative. The melting point was checked to determine the purity of the product. The structure elucidation was carried out by using spectroscopic method (uvvis, IR, 1HNMR and MS) The result showed that the highest yield of LR1 was obtained around 15 % when 0.25 % of chloric acid used as catalyst. The higher the condition of acid in the reaction, the lower the tield of LR1 obtained
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL
Full text linkLR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione; Leni Ritmaleni 2), which designed and assumed to have biologically activity as anticancer, has been successfully synthesized by using the Biginelli reaction. This research was aimed to investigate the effect of benzaldehyde excess in the synthesis of LR-2 and to evaluate the cytotoxic activity of LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together with thiourea at one time as said as one pot reaction synthetic methodology and the reaction was acid catalysed. The mole equivalent of benzaldehyde was in excess compare to others. The effect of benzaldehyde in excess is the higher the mole of benzaldehyde, the lower the yield of LR-2. The cytotoxicity of LR-2 was done by using MTT method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa
OXIDATION KINETICS AND QUANTIFICATION METHOD OF CHOLESTEROL USING CHOLESTEROL OXIDASE ENZYME CATALYST
Full text linkIn view of health, cholesterol is believed as one of many sources can raise several diseases. Hence, both of research in quantification and developing simple, rapid and accurate analysis method of cholesterol in a sample is very important. Aim of this research was to investigate cholesterol oxidation kinetics and its quantification method based on oxidation of cholesterol and formation complex compound of hexathiocyanato ferat(III), {Fe(SCN)6}-3. The kinetics analysis and quantification, involved cholesterol oxidation in 0.1 M and pH 7.0 phosphate buffer solution to produce cholest-4-en-3-one and hydrogen peroxide, in the presence of cholesterol oxidase enzyme. The formed hydrogen peroxide was used to oxidize iron(II) ion, which was reacted furthermore with thiocyanate ion to raise the red-brown complex compound. Results of the study showed that the complex was stable at 10-120 min since the reaction was started, with maximum wavelength of 530-540 nm. While the kinetics analysis gave first order oxidation reaction with a reaction rate constant, kapp = 5.22 x 10-2 min-1. Based on this kinetics data, cholesterol analysis method could be developed i.e. by oxidizing cholesterol within 1.5 h using cholesterol oxidase as a catalyst, and then reacted with Fe2+, in a solution containing thiocyanate ion. Absorbencies of solutions of the complex compound, measured at wavelength of 535 nm, were linearly proportional to their cholesterol concentrations, in the range of 50-450 ppm.
Keywords: cholesterol, quantification, kinetics, hexathiocyanato ferat(III
Iron (III) chloride, Aluminium chloride and Zinc chloride as Catalysts in the Synthesis of Tetrahydropentagamavunon-0
Full text linkTetrahydropentagamavunon-0 (THPGV-0), an analog of tetrahydrocurcumin, has chemical name as 2,5-bis(4'-hydroxy-3'-methoxybenzyl)-cyclopentanone. It can be synthesised from pentagamavunon-0 (PGV-0, 2,5-bis(4'-hydroxy-3'-methoxybenzylidene)-cyclopentanone). The hydrogenation reaction was applied in this synthesis and it was catalysed by palladium on carbon which yielded THPGV-0 in moderate yield. In seeking for a cheap catalyst, the different catalysts was applied in this hydrogenation reaction. The reaction condition was changed by using different catalysts other than palladium on carbon. Among Palladium/carbon (Pd/C) 10 %, Iron (III) chloride (FeCl3), Aluminium chloride (AlCl3) dan Zinc chloride (ZnCl2) only reaction catalysed by Pd/C gave THPGV-0. While others gave products like the side products when Pd/C used. Structure elucidation was carried out by using spectroscopic method. The obtained products from the reaction by using FeCl3, and AlCl3 as catalysts are 1,3-bis(4'-hydroxy-3'-methoxybenzyl)cyclopentane and 2,5-bis(4'-hydroxy-3'-methoxybenzyl) cyclopentanol and by using ZnCl2 as catalyst is 1,3-bis(4'-hydroxy-3'-methoxybenzyl)cyclopentane. Keywords: Iron (III) chloride, Aluminium chloride, zinc chloride, tetrahydropentagamavunon-
ATTEMPTED SYNTHESIS OF BIS-SPIROEPOXIDE DITHIANEDIOXIDE
Full text linkThe bis-spiroepoxide dithiane dioxide has been attempted to be synthesised as its role as an intermediate in the synthesis of diaminopimelic acid (DAP). The first method was carried out by reacting the 3-dithiane-2- diethylphosphonate 4 with an aqueous solution of the commercially available glutaraldehide resulting the bis-ketene dithiane dioxide 5. The second alternative method was involving the ozonolysis of cyclopentene 7 in the synthesis of bis-ketene dithiarie dioxide 5 in four step reactions which gave moderate to good yield. Unfortunately, epoxidation process for the bis-ketene dithiane dioxide 5 was still unsuccess yet
Sintesis Tetrahidro pentagamavunon-O Synthesis of Tetrahydro pentagavunon-O
Full text linkKurkumin, tetrahidrokurkumin, dan turunan kurkumin struktur monoketon, pentagamavunon-O (PGV-O), telah banyak diteliti dan terbukti mempunyal aktivitas antioksidan, antiinflamasi dan antikanker. Upaya dalam pengembangan obat baru selanjutnya adalah sintesis senyawa Tetrahidro Pentagamavunon-O (THPGV-O)yang diperkirakan sebagal metabolit aktif dari mO. Metode sintesis THPGV-O adalah dengan cara hidrogenasl PGV-O menggunakan katalis Pd/C 10 % dan gas hldrogen pada suhu kamar. Hasil sintesis . ,dianalisis berdasarkan titik lebur, kromatogram KLTdan GC, dan elusidast struktur menggunakan metode spektrometri UV, IR, Massa, lH-NMR, 13C-NMR. Dari hasil penelitian diperoleh bahwa senyawa THPGV-O berbentuk serbuk putih dengan jarak lebur 122-123° DC. Rendemen hasil sintesis adalah 25 %
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