2,151 research outputs found
The Tarantula--Revealed by X-rays (T-ReX) second try
No full textSupplemental data products for the journal article "The Tarantula--Revealed by X-rays (T-ReX)," to be published in the Astrophysical Journal Supplements in 2023.
Please see README.txt for a description of the contents of this archive
Phosphorylation of Two Serine Residues Regulates Human T-Cell Leukemia Virus Type 2 Rex Function
No full textABSTRACT
The function of the human T-cell leukemia virus (HTLV) Rex phosphoprotein is to increase the level of the viral structural and enzymatic gene products expressed from the incompletely spliced viral RNAs containing the Rex-responsive element. The phosphorylation of HTLV type 2 Rex (Rex-2), predominantly on serine residues, correlates with an altered conformation, as detected by a gel mobility shift, and is required for specific binding to its viral RNA target sequence. Thus, the phosphorylation state of Rex in the infected cell may be a switch that determines whether the virus exists in a latent or a productive state. A mutational analysis of Rex-2 that focused on serine and threonine residues was performed to identify regions or domains within Rex-2 important for function, with a specific emphasis on identifying Rex-2 phosphorylation mutants. We identified mutations near the carboxy terminus that disrupted a novel region or domain and abrogated Rex-2 function. Mutant M17 (with S151A and S153A mutations) displayed reduced phosphorylation that correlated with reduced function. Replacement of both serine residues 151 and 153 with phosphomimetic aspartic acid restored Rex-2 function and locked Rex-2 in a phosphorylated active conformation. A mutant containing threonine residues at positions 151 and 153 displayed a phenotype indistinguishable from that of wild-type Rex. Furthermore, this same mutant showed increased threonine phosphorylation and decreased serine phosphorylation, providing conclusive evidence that one or both of these residues are phosphorylated in vivo. Our results provide the first direct evidence that the phosphorylation of Rex-2 is important for function. Further understanding of HTLV Rex phosphorylation will provide insight into the regulatory control of HTLV replication and ultimately the pathobiology of HTLV.</jats:p
Phosphorylation regulates human T-cell leukemia virus type 1 Rex function
No full textAbstract Background Human T-cell leukemia virus type 1 (HTLV-1) is a pathogenic complex deltaretrovirus, which is the causative agent of adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis. In addition to the structural and enzymatic viral gene products, HTLV-1 encodes the positive regulatory proteins Tax and Rex along with viral accessory proteins. Tax and Rex proteins orchestrate the timely expression of viral genes important in viral replication and cellular transformation. Rex is a nucleolar-localizing shuttling protein that acts post-transcriptionally by binding and facilitating the export of the unspliced and incompletely spliced viral mRNAs from the nucleus to the cytoplasm. HTLV-1 Rex (Rex-1) is a phosphoprotein and general protein kinase inhibition correlates with reduced function. Therefore, it has been proposed that Rex-1 function may be regulated through site-specific phosphorylation. Results We conducted a phosphoryl mapping of Rex-1 over-expressed in transfected 293 T cells using a combination of affinity purification and liquid chromatography tandem mass spectrometry. We achieved 100% physical coverage of the Rex-1 polypeptide and identified five novel phosphorylation sites at Thr-22, Ser-36, Thr-37, Ser-97, and Ser-106. We also confirmed evidence of two previously identified residues, Ser-70 and Thr-174, but found no evidence of phosphorylation at Ser-177. The functional significance of these phosphorylation events was evaluated using a Rex reporter assay and site-directed mutational analysis. Our results indicate that phosphorylation at Ser-97 and Thr-174 is critical for Rex-1 function. Conclusion We have mapped completely the site-specific phosphorylation of Rex-1 identifying a total of seven residues; Thr-22, Ser-36, Thr-37, Ser-70, Ser-97, Ser-106, and Thr-174. Overall, this work is the first to completely map the phosphorylation sites in Rex-1 and provides important insight into the regulation of Rex-1 function.</p
Measuring industry-science links through inventor-author relations: A profiling method
No full textIn this pilot study we examine the performance of text-based profiling in recovering a set of validated inventor-author links. In a first step we match patents and publications solely based on their similarity in content. Next, we compare inventor and author names on the highest ranked matches for the occurrence of name matches. Finally, we compare these candidate matches with the names listed in a validated set of inventor-author names. Our text-based profile methodology performs significantly better than a random matching of patents and publications, suggesting that text-based profiling is a valuable complementary tool to the name searches used in previous studies.innovation; industry-science links; text-based profiling;
Strike Up the Band! The Legacy of Patrick S. Gilmore
No full textabstract: Patrick Gilmore was an Irish-American bandmaster who re-established the woodwinds in wind bands and developed one of the best professional bands in the world. He died the same year Sousa started his professional band, 1892, and his bands served as the prototype for Sousa's and countless other amateur and professional bands
Sitespecific phosphorylation regulates human T-cell leukemia virus type 2 Rex function in
No full textHuman T-cell leukemia virus type 2 (HTLV-2) Rex is a transacting regulatory protein required for efficient cytoplasmic expression of the unspliced and incompletely spliced viral mRNA transcripts encoding the struc-tural and enzymatic proteins. Previously, it was demonstrated that phosphorylation of Rex-2, predominantly on serine residues, is correlated with an altered conformation, as observed by a gel mobility shift and the detection of two related protein species (p24Rex and p26Rex). Rex-2 phosphorylation is required for specific binding to its viral-mRNA target sequence and inhibition of mRNA splicing and may be linked to subcellular compartmentalization. Thus, the phosphorylation-induced structural state of Rex in the infected cell may be a switch that determines whether HTLV exists in a latent or productive state. We conducted a phosphoryl and functional mapping of both structural forms of mammalian-cell-expressed Rex 2 using affinity purification, liquid chromatography-tandem mass spectrometry, and site-directed substitutional mutational analysis. We identified two phosphorylation sites in p24Rex at Ser-117 and Thr-164. We also identified six phosphorylatio
The poetical works of Patrick Hannay, A.M. MDCXXII : with a memoir of the author.
No full text"A faithful and literal re-publication" of the rare 1622 edition of Hannay's collected poems. With facsimile of original engraved t.-p.: The Nightingale. Sheretine and Mariana. A happy husband. Eligies on the death of Queene Anne. Songs and Sonnets by Patrick Hanay gent. London printed for Nathaniel Butler. 1622."Memoir of Patrick Hannay, A.M., by David Laing...": p. 7-47."Presented to the members of the Hunterian club by Thomas Russell."Philomela, the nightingale.--Sheretine and Mariana.--A happy husband: or, Directions for a maid to chvse her mate. Together with A wives behaviovr after mariage. The second edition.--Elegies, on the death of ovr late soueraigne Qveene Anne. With epitaphs.--Songs and sonnets.Mode of access: Internet
Sketches of the life and character of Patrick Henry /
No full textTitle vignette (portrait of author) on engr. t.-p.Added illustrated t.p. reads : The life of Patrick Henry. Rev. ed., 1846.Mode of access: Internet
X-ray properties of early-type stars in the Tarantula Nebula from T-ReX
No full textWe reassess the historical L-X/L-Bol relation for early-type stars from a comparison between T-ReX, the Chandra ACIS X-ray survey of the Tarantula Nebula in the Large Magellanic Cloud (LMC), and contemporary spectroscopic analysis of massive stars obtained primarily from VLT/FLAMES, VLT/MUSE, and HST/STIS surveys. For 107 sources in common (some host to multiple stars), the majority of which are bolometrically luminous (40 per cent exceed 10(6)L(circle dot)), we find an average log L-X/L-Bol = -6.90 +/- 0.65. Excluding extreme systems Mk 34 (WN5h+WN5h), R140a (WC4+WN6+), and VFTS 399 (O9 IIIn+?), plus four WR sources with anomalously hard X-ray components (R130, R134, 8135, Mk 53) and 10 multiple sources within the spatially crowded core of R136a, log L-X/L-Bol = -7.00 +/- 0.49, in good agreement with Galactic OB stars. No difference is found between single and binary systems, nor between O, Of/WN, and WR stars, although there does appear to be a trend towards harder X-ray emission from O dwarfs, through O (super)giants, Of/WN stars, and WR stars. The majority of known OB stars in the Tarantula are not detected in the T-ReX point source catalogue, so we have derived upper limits for all undetected OB stars for which log L-Bol/L-circle dot \u3e= 5.0. A survival analysis using detected and upper limit log L-X/L-Bol values indicates no significant difference between luminous O stars in the LMC and the Carina Nebula. This analysis suggests that metallicity does not strongly influence L-X/L-Bol. Plasma temperatures for single, luminous O stars in the Tarantula ((kT(m)) over bar = 1.0 key) are higher than counterparts in Carina ((kT(m)) over bar = 0.5 keV)
Chas. White, author of Story of Australian bushranging ; Dr. Pechey, present at Keightly episode ... [picture] /
No full textIncludes portrait of Captain Starlight from: Robbery under arms / by Rolf Boldrewood.; Exhibited: "Bushrangers and Bandits", National Museum of Australia, June 2002 - June 2003.; Exhibited: "In the Line of Duty: Policing in Australia 1788 - 2006", Old Parliament House, August 2006 - February 2007. AuCNL. Portraits and inscriptions (top L to R): Chas. White author of Story of Australian bushranging; Dr. Pechey present at Keightly episode; Hipkiss gave Ben Hall first death wound at Billabong 5th May -65; Ver. Rev. Dr. Gibney heroic rescue at Glenrowen [Glenrowan]; Superintnt Hare author of Last of the bushrangers; Ben Hall; Lowry; Starlight; T. Clarke; Rutherford; Martin Cash Tasmanian outlaw after sentence lived a respected farmer; Const. Bracken escaped from Kellys Glenrowen [Glenrowan]; Insr Stephenson daring capture & shooting of Lowry; McKinley [McKinlay?], Burns & Day daring shooting & capture of Angel & Thurston; J. Hawthorne now a squatter Wee Waa daring capture of Dunne
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