173 research outputs found
Francois Ludger Diard poetry notebook and Mobile scrapbook, W.0025
No full textAbstract: Notebook of this Mobile, Alabama, native's poetry and a scrapbook of newspaper clippings and letters relating to his work.Scope and Content Note: This collection contains a notebook of Diard's poetry and a scrapbook of newspaper clippings and letters relating to his work. Diard annotated many of the poems collected in this 200-page notebook, noting when they were written and/or published. The majority of the notebook contains clippings of poetry published by local newspapers during World War I. Most of these poems are patriotic, praising the sacrifices of Alabama servicemen. Toward the back of the volume are several letters to Diard from the vice-president of the
Mobile Register, Erwin Craighead, soliciting poems for an anthology of Mobile literati.The Mobile scrapbook contains newspaper items clipped from the
Mobile Register, the
Mobile Times, and other Mobile newspapers. The articles were published between 1849 and 1945, with most of the articles published between 1929 and 1945. While most of the items address city events and Mobile's literary community, other items include obituaries and state and national news briefs. Approximately one-quarter of the articles clipped are copies of Erwin Craighead's column "Dropped Stitches From Mobile's Past."Biographical/Historical Note: Francois Ludger Diard, son of Charles August R. and Sarah Antonia Ludgere Diard, was born on 29 October 1883 in Mobile, Alabama. He was the author of many poems, including "We Have Kept the Faith," a response to "We Shall Not Sleep, Tho Poppies Grow in Flanders Fields" written by Lieutenant Colonel Dr. John McRae of Canada while the second battle of Ypres, Belgium, was in progress. He also wrote
The Tree: Being the Strange Case of Charles R. S. Boyington about the 1835 Mobile murder of Nathaniel Frost by Charles Boyington. Diard died in Mobile on 25 March 1955
Ethical principles and standards for the conduct of human and animal biological rhythm research
No full textMost research papers published in Chronobiology International report the findings of investigations conducted on laboratory animals and human beings. The Journal, its editors and the publication committee endorse the compliance of investigators to the principles of the Declaration of Helsinki of the World Medical Association relating to the conduct of ethical research on human beings and the Guide for the Care and Use of Laboratory Animals of the Institute for Laboratory Animal Research of the National Research Council relating to the conduct of ethical research on laboratory and other animals. Chronobiology International requires that submitted manuscripts reporting the findings of human and animal research conform to the respective policy and mandates of the Declaration of Helsinki and the Guide for the Care and Use of Laboratory Animals. The peer review of manuscripts will thus include judgment of whether or not the involved research methods conform to the standards of good research practice. This article outlines the basic expectations for the methods of human and animal biological rhythm research, both from the perspective of the fundamental criteria necessary for quality chronobiology investigation and from the perspective of humane and ethical research on human beings and animals
Formation of morphological differentiation patterns in the ascomycete Neurospora crassa
No full textDeutsch A, Dress A, Rensing L. Formation of morphological differentiation patterns in the ascomycete Neurospora crassa. Mechanisms of Development. 1993;44(1):17-31.Morphological differentiation patterns - among them concentric rings and radial zonations - can be induced in the band-mutant of Neurospora crassa by appropriate experimental conditions, in particular by a mere shift of certain salt concentrations in the medium. The role of initial experimental conditions is examined and, furthermore, the influences of artificially induced phase differences are analyzed with respect to pattern formation. While the concentric ring pattern is due to some (endogenous) circadian rhythmicity within every hypha, nothing is known about the underlying mechanism of radial zonation development. Various hypotheses were tested with the help of a cellular automaton model which mimicks growth, branching and differentiation of a fungal mycelium. In particular, sufficient conditions are provided which imply the formation of radial spore zonations. These conditions postulate a rather homogeneous microscopic hyphal branching pattern and induction of spore differentiation by means of an activator-inhibitor system. Furthermore, a working hypothesis for the formation of spore patterns in Neurospora crassa is suggested which is based on an extracellular control of fungal differentiation
Valproic acid has temporal variability in urinary clearance of metabolites
No full textThe reasons for the intra- and interindividual variability in the clearance of valproic acid (VPA) have not been completely characterized. The aim of this study was to examine day-night changes in the clearance of 3-oxo-valproate (3-oxo-VPA), 4-hydroxy-valproate (4-OH-VPA), and valproic acid glucuronides under steady state. Six diurnally active healthy male volunteers ingested 200 mg sodium valproate 12 hourly, at 0800 and 2000, for 28 days. On the last study day, two sequential 12-h urine samples were collected commencing at 2000 the evening before. Plasma samples were obtained at the end of each collection. Following alkaline hydrolysis, urine was analyzed for concentrations of VPA, 3-oxo-VPA, and 4-OH-VPA. A separate aliquot was assayed for creatinine (CR). The plasma concentrations of VPA, 3-oxo-VPA, 2-en-VPA, and CR were determined. The analysis of VPA and its metabolites was performed by CC-MS. There was an increase in plasma 3-oxo-VPA concentration at 0800, sampling as compared to 2000 sampling (p < .05). The urinary excretion of 3-oxo-VPA and VPA glucuronides were decreased between 2000 and 0800, compared to between 0800, and 2000, by 30% and 50% respectively (p < .05). These results indicate a nocturnal decrease in renal clearance of 3-oxo-VPA rather than a decrease in the beta -oxidation of VPA at night. These differences were not explained by differences between the sampling periods in CR excretion. These results indicate the importance of collecting samples of 24-h duration when studying metabolic profiles of VPA
Quantitative Analyses of the HMG expression in selected malign neoplasias
No full textThe high mobility group (HMG) proteins HMGA1, HMGA2, and HMGB1 have been discussed as potential tumour markers for years. They belong to the group of HMG proteins acting as architectural transcription factors regulating chromatin structure and gene expression. Over- or re-expression of these embryonic proteins have been detected in various malignancies.Since at the outset of this work in the year 2002 no quantitative analyses of HMGA expression in malignant tumours existed, it was the aim of this study to examineHMGA2 expression in solid tumours and in the peripheral blood of patients mainly with malignant neoplasias using quantitative real-time RT-PCR (qRT-PCR). Absolute quantification of HMGA2 expression in matching tumourous and non-tumourous lung tissue of 34 non-small cell lung cancer patients revealed a highly significant overexpression in the adenocarcinoma as well as in the squamous cell carcinoma samples. In some of the non-neoplastic tissues elevated levels of HMGA2 transcripts were detected which could be due to premalignant changes frequently observed in bronchoalveolar epithelium of smokers and/or lung cancer patients. In 16 canine prostatic tissues with diverse histologic findings a correlation of HMGA2 level and malignant potential was apparent using an accordingly modified qRT-PCR protocol.These results underline the relevance of HMGA2 expression as diagnostic and prognostic marker in cancer therapy.By relative quantification using qRT-PCR HMGA2 expression could be detected in the peripheral blood of all 22 mamma carcinoma patients tested, which is in contrast to previous studies with conventional RT-PCR. Furthermore, no significant differences of HMGA2 expression were detected between patients with or without metastatic disease, indicating that HMGA2 expression in the peripheral blood is not applicable as prognostic marker for breast cancer. qRT-PCR analyses of 36 blood samples from patients with solid tumours, malign diseases of the haematopoietic andlymphatic system, and various autoimmune diseases compared to seven samples of healthy donors showed a strong HMGA2 over-expression solely for one CML patient. Based on this result a study on 56 patients with diseases of the haematopoietic and lymphatic system was conducted. For the leukaemia patients in general as well as for the AML patients in particular a significant over-expression of HMGA2 could be shown. In addition, for the twelve CML patients examined a positive correlation of HMGA2 mRNA transcript levels and white blood cell count at the time of blood drawl was found.Since it was shown that HMGB1 can sensitise cancer cells to cisplatin and this cytostatic drug is commonly used for the treatment of osteosarcomas of the dog, HMGB1 expression was examined in seven canine sarcomas. Preceding sequence analyses revealed a strong similarity of the canine HMGB1 gene and the deduced protein to the human orthologs. Northern Blot analysis and semi-quantitative RT-PCR consistently showed a clear variation of HMGB1 expression, which could be of importance for further therapeutic protocols using cisplatin. Apart from the nuclear function of the HMGB1 protein as architectural transcription factor, extra cellular HMGB1 plays an important role as mediator of inflammation and in tumour metastasis. Herein the angiogenic potential of recombinant HMGB1 protein was shown in vitro for the first time. Application of HMGB1 induced cell migration and sprouting in an endothelial sprouting assay in a dose dependent manner
Investigations on the molecular pathogenesis of follicular thyroid tumors
No full textThyroid tumors belong to the most frequent epithelial tumors. On the basis of their histologically and clinically definable forms and stages they represent an outstanding model system for tumorgenesis. Cytogenetic analyses can serve here as an excellent and efficient tool for cloning tumor-relevant genes. Known important cytogenetic changes in adenomas are trisomy 7 as well as breaks in chromosomal bands 19q13.4 and 2p21. Starting from these data, in the present work the breakpoint cluster 19q13.4 was cloned. The newly found upstream lying zinc finger gene ZNF331 was described as a candidate-gene. The second relevant breakpoint cluster in band 2p21 was cloned as well. Here the newly found candidate gene THADA was described which fuses through translocations to different other chromosomes, creating a truncated fusion protein. It could clearly be shown that the HMGA2 gene has an influence on the tumor progression of epithelial thyroid gland cells. Since in animal tissues HMGA2, as shown in mice, is only active during the embryonal stages, the issue arises whether an observed overexpression evokes a tumorigenic status and therefore might be a suitable central point of application for a therapy
European Union - The Second Founding
No full textProf. Dr. Ludger Kühnhardt, Director at the renowned Center for European Integration Studies (ZEI) at the University of Bonn is presenting a broadly structured study about the first fifty years of European integration, its geopolitical context and academic reflection. His study is based on the two-fold thesis that since a few years, the European Union is going through a process of its Second Founding while simultaneously changing its rationale. The original founding of European integration in 1957 was based on the notion of internal reconciliation among European states and societies. Since the 1990""s European integration has become, increasingly, a political project with implications for the internal structure of its member states and their societies. At the same time, with the end of the Cold War, the rational of European integration has begun to change: European integration is about a new global role of Europe, its contribution to the management of global affairs and its ability to cope with the effects of globalization on Europe. Inside the EU, the Second Founding is about a new contract between political elites and the people of Europe in order to solidify legitimacy and effectiveness for this unique experiment in European history. Prof. Dr. Ludger Kühnhardt has been Director at the Center for European Integration Studies (ZEI) since 1997. He is author of thirty books and edits the volumes of ZEI at Nomos
Musterbildung und Mustererkennung. Hrsg. von Werner Köhler. Nova Acta Leopoldina. Band 67 Nummer 281. Deutsche Akademie der Naturforscher Leopoldina, Halle (Salle), Barth Verlagsges., Leipzig 1992. 418 Seiten, 252 Abb., 4 Tab., DM 78,—. ISBN 3‐335.00301‐2, ISSN 0369‐5034
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