1,721,005 research outputs found
Novel insights into the metal binding ability of ZinT periplasmic protein from Escherichia coli and Salmonella enterica
No full textThe ZinT mediated Zn(ii) uptake is one of the major differences in the metabolism of human and bacterial cells that can be challenged when looking for possible highly selective metal-based therapeutics. ZinT is a 216-amino acid periplasmic protein expressed by Gram-negative bacteria, which shuttles Zn(ii) ions to the ZnuABC transporter under zinc-limiting conditions. The suggested metal-binding sites of ZinT correspond to a domain containing three highly conserved histidine residues (His 167, 176 and 178) and to the N-terminal histidine-rich loop HGHHXH (residues 24-29). The coordination chemistry of the ZinT complexes with Zn(ii) and Cu(ii) has been investigated. The present work is focused on the protected peptides Ac-24HGHHSH29-NH2 and Ac-166DHIIAPRKSSHFH178-NH2 as models for the putative metal binding sites of ZinT from Escherichia coli (EcZinT), and Ac-24HGHHAH29-NH2 and Ac-166DHIIAPRKSAHFH178-NH2 from the ZinT protein expressed by Salmonella enterica sv. Typhimurium (SeZinT). The investigated peptides are able to form stable mono-nuclear complexes where the histidine residues represent the principal metal anchoring sites. The ZnuA (a periplasmic component of the ZnuABC transporter) metal binding site exhibits higher affinity for Zn(ii) than ZinT, suggesting that the interaction of the two proteins through the formation of a binary complex may involve the metal transfer from ZinT to ZnuA. In contrast, this would not occur in Cu(ii), since the ZinT complexes are more stable. Furthermore, at acidic pH, where the antimicrobial peptide calcitermin is biologically active, it also binds the metal ions with higher affinity than ZinT, representing a possible efficient competitor and antagonist of ZinT in the host human organism
Deferoxamine B: A Natural, Excellent and Versatile Metal Chelator
No full textDeferoxamine B is an outstanding molecule which has been widely studied in the past decade for its ability to bind iron and many other metal ions. The versatility of this metal chelator makes it suitable for a number of medicinal and analytical applications, from the well-known iron chelation therapy to the most recent use in sensor devices. The three bidentate hydroxamic functional groups of deferoxamine B are the centerpiece of its metal binding ability, which allows the formation of stable complexes with many transition, lanthanoid and actinoid metal ions. In addition to the ferric ion, in fact, more than 20 different metal complexes of deferoxamine b have been characterized in terms of their chemical speciation in solution. In addition, the availability of a terminal amino group, most often not involved in complexation, opens the way to deferoxamine B modification and functionalization. This review aims to collect and summarize the available data concerning the complex-formation equilibria in solutions of deferoxamine B with different metal ions. A general overview of the progress of its applications over the past decade is also discussed, including the treatment of iron overload-associated diseases, its clinical use against cancer and neurodegenerative disorders and its role as a diagnostic tool
Binary and ternary Cu(II) complexes of L-spinacine in aqueous solution
No full textComplexation of L-spinacine with the Cu(II) ion in aqueous solution, even in the presence of L/D-phenylalanine, L/D-tryptophane or L/D-histidine, is thoroughly investigated by means of potentiometry and UV-VIS spectrophotometry. The synthesis of L-spinacine by reaction of L-histidine with formaldehyde is also described in detail
Interaction of divalent cations with Park9 protein
No full textWe have tested two peptide sequences from Park9 Parkinson’s disease (PD) gene, -P1D2E3K4H5E6L7- and -F1C2G3D4G5A6N7D8C9G10- (2), for Mn(II), Zn(II) and Cu(II) binding. Park9 encoded protein can protect cells from manganese poisoning, which is an environmental risk factor for a Parkinson’s disease-like syndrome. In fact, Park9 belongs to a family of ATP-ases involved in metal coordination and transportation; familial gene mutations may result in early development of PD. The chosen fragments are located from 1165 to 1171 and from 1184 to 1193 residues in the Park9 sequence, and are highly conserved in a number of organisms, going from yeasts to humans. To understand the details of their metal binding sites at different pH values and at different ligand to metal molar ratios, mono- and multidimensional NMR spectroscopy, together with potentiometric and UV-vis experiments, have been used, showing that the three metals are able to effectively bind the examined peptides. Mn(II) and Zn(II) coordination with peptide involves imidazol Nε or Nδ of His5 and carboxyl γ-O of Asp2, Glu3 and Glu6 residues. Six donor atoms participate in Mn(II) binding, resulting in a distorted octahedral geometry, possibly involving bidentate interaction of carboxyl groups; four donor atoms participate in Zn(II) binding, resulting in a tetracoordinated geometry. Mn(II) and Zn(II) coordination involves the two cysteine residues with peptide; Mn(II) accepts additional ligand bonds from D4 and D8 to complete the coordination sphere, together with some water molecules
Solute - solute - solvent interactions in dilute ternary aqueous solutions of urea and α,ω-aliphatic diols
No full textThe enthalpies of dilution and osmotic coefficients of ternary solutions of urea and α,ω-aliphatic diols in water have been determined at 298 K through microcalorimetric and vapour pressure osmometric measurements, respectively. The experimental virial coefficients of the power expansion series of the excess Gibbs energies and enthalpies have been evaluated and compared with the literature data for similar aqueous systems containing urea and alkanols, ketones and cyclic ethers. The following diols have been considered: propane-diol-1,3; butane-diol-1,4; pentane-diol-1,5. The results are discussed in terms of the hydrophilic and hydrophobic properties of the solutes, and in the light of previous data on the excess thermodynamic properties of binary water-aliphatic diol solutions
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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