565 research outputs found

    Two "classical" Old Yellow Enzymes from Chryseobacterium sp CA49: Broad substrate specificity of Chr-OYE1 and limited activity of Chr-OYE2

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    Two putative Old Yellow Enzyme (OYE) homologues, Chr-OYE1 and Chr-OYE2, were identified from the genome of Chryseobacterium sp. CA49 as new members of the "classical" subfamily. Chr-OYE1 and Chr-OYE2 were most closely related to the SYE4 from Shewanella oneidensis and NerA from Agrobacterium radiobacter with 41% and 45% identity, respectively. Both enzymes were expressed in Escherichia coli in soluble form, but their catalytic abilities as ene-reductases were quite different. Among the 19 substrate tested, Chr-OYE1 could catalyze the reduction of 18 of them including an ynone with excellent stereos electivity for several prochiral ones, and its specific activity was roughly 1100-fold high than Chr-OYE2, which only catalyzed 3 of the substrates. After restoring the conserved tyrosine, Chr-OYE2 remained the same substrate spectrum, but showed significantly enhanced activity and stereoselectivity. (C) 2015 Elsevier B.V. All rights reserved

    Musical programming in mainstream radio stations (including the CHR format)

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    Z doświadczeń dziennikarzy muzycznychThe author describes the issue of musical programming in mainstream radio stations (based on CHR format). He defines the most popular radio formats, presents the history of programming, and points out the basic mechanisms that every programmer should remember.Autor opisał zagadnienie programowania muzycznego stacji mainstreamowych (w oparciu o format CHR). Zdefiniował najpopularniejsze formaty radiowe, przybliżył ich historię, wspomniał o podstawowych mechanizmach, o których muszą pamiętać wszyscy programiści muzyczni.Publikacja dofinansowana przez Wydział Filologiczny U

    Review of the realisation of Norway’s “Strategy for intensifying international efforts for the elimination of female genital mutilation for the period 2014–2017”

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    This report was commissioned by Norad and the evaluation was realised by Chr. Michelsen Institute (CMI). Joar Svanemyr is the main author of the report. Camilla Gianella at CMI and Ragnhild Elise Johansen, external consultant to CMI, provided inputs to drafts of the report. Norad reviewed and commented on the draft report but the views and opinions expressed in this report are those of the author and do not necessarily reflect the official policy or position of Norad

    Review of the realisation of Norway’s “Strategy for intensifying international efforts for the elimination of female genital mutilation for the period 2014–2017”

    No full text
    This report was commissioned by Norad and the evaluation was realised by Chr. Michelsen Institute (CMI). Joar Svanemyr is the main author of the report. Camilla Gianella at CMI and Ragnhild Elise Johansen, external consultant to CMI, provided inputs to drafts of the report. Norad reviewed and commented on the draft report but the views and opinions expressed in this report are those of the author and do not necessarily reflect the official policy or position of Norad

    Review

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    Review

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    Mercè Rodoreda, the most translated author in Catalan fiction

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    Mercè Rodoreda is the most translated author in Catalan literature. La Plaça del Diamant, which has been translated into 37 languages in dozens of different translations, places her ahead of such internationally famous fiction writers as Jaume Cabré and Albert Sánchez Piñol. This symbolic novel of the human condition, hailed by critics and beloved by a diverse array of readers, is particularly representativ of the author’s literary talent and ambition and can be considered a major 20th-century classic thanks to its extraordinarily high quality.Keywords: Catalan literature, translation, fiction, Mercè Rodoreda, La Plaça del Diamant
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