70 research outputs found
Our glorious dead [music] : memorial song to British Empire /
No full textCover title.; "This beautiful song should be sung on all memorial occasions it is dedicated to our heroes who fell in the great war".; Also available online http://nla.gov.au/nla.mus-an5528003; N, M 160818
A strain tensor method for three-dimensional optimal Michell structures
No full textThesis: M. Eng., Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, 2016.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (pages 94-95).In the design of discrete structures such as trusses and frames, important quantitative goals such as minimal weight or minimal compliance often dominate. Many numerical techniques exist to address these needs. However, an analytical approach exists to meet similar goals, which was initiated by A.G.M. Michell (1904) and has been mostly used for two-dimensional structures so far. This thesis develops a method to extend the existing mainly two-dimensional approach to apply to three-dimensional structures. It will be referred as the Michell strain tensor method (MSTM). First, the proof that MSTM is consistent with the existing theory in two dimensions is provided. Second, two-dimensional known solutions will be replicated based on MSTM. Finally, MSTM will be used to solve new three- dimensional cases.by Benjamin Jacot.M. Eng
Chondrolectin mediates growth cone interactions of motor axons with an intermediate target
Get PDFThe C-type lectin chondrolectin (chodl) represents one of the major gene products dysregulated in spinal muscular atrophy models in mice. However, to date, no function has been determined for the gene. We have identified chodl and other novel genes potentially involved in motor axon differentiation, by expression profiling of transgenically labeled motor neurons in embryonic zebrafish. To enrich the profile for genes involved in differentiation of peripheral motor axons, we inhibited the function of LIM-HDs (LIM homeodomain factors) by overexpression of a dominant-negative cofactor, thereby rendering labeled axons unable to grow out of the spinal cord. Importantly, labeled cells still exhibited axon growth and most cells retained markers of motor neuron identity. Functional tests of chodl, by overexpression and knockdown, confirm crucial functions of this gene for motor axon growth in vivo. Indeed, knockdown of chodl induces arrest or stalling of motor axon growth at the horizontal myoseptum, an intermediate target and navigational choice point, and reduced muscle innervation at later developmental stages. This phenotype is rescued by chodl overexpression, suggesting that correct expression levels of chodl are important for interactions of growth cones of motor axons with the horizontal myoseptum. Combined, these results identify upstream regulators and downstream functions of chodl during motor axon growth
Water-based ecology: A First Nations' proposal to repair the definition of a forest ecosystem
No full textFirst Nations Elders are very concerned about whether enough clean drinking water will exist for future generations. Three highly respected Elders from the Southern Interior of British Columbia helped the author investigate First Nations water-based ecology: Mary Thomas from the Secwepemc (Shuswap), Millie Michell from the Nlaka'pamux (Thompson), and Mary Louie from the Syilx (Okanagan) Nation. This paper follows on from the author's previous examination of First Nations' spiritual and ecological perspectives on water (BC Journal of Ecosystems and Management 1(1):54-68). The Elders' vision of the relationships between water, land, and animals highlights an apparent shortcoming in Western science's definition of an ecosystem. In this paper, the author encourages a shift towards water-based ecosystem management, proposing to repair of the definition of forest ecosystems in a way that interweaves First Nations' philosophy with Western science's ecosystem-based management approach
Characterization of water and wildlife strains as a subgroup of Campylobacter jejuni using DNA microarrays.
No full textCampylobacter jejuni is the leading cause of human bacterial gastroenteritis worldwide, but source attribution of the organism is difficult. Previously, DNA microarrays were used to investigate isolate source, which suggested a non-livestock source of infection. In this study we analysed the genome content of 162 clinical, livestock and water and wildlife (WW) associated isolates combined with the previous study. Isolates were grouped by genotypes into nine clusters (C1 to C9). Multilocus sequence typing (MLST) data demonstrated that livestock associated clonal complexes dominated clusters C1-C6. The majority of WW isolates were present in the C9 cluster. Analysis of previously reported genomic variable regions demonstrated that these regions were linked to specific clusters. Two novel variable regions were identified. A six gene multiplex PCR (mPCR) assay, designed to effectively differentiated strains into clusters, was validated with 30 isolates. A further five WW isolates were tested by mPCR and were assigned to the C7-C9 group of clusters. The predictive mPCR test could be used to indicate if a clinical case has come from domesticated or WW sources. Our findings provide further evidence that WW C. jejuni subtypes show niche adaptation and may be important in causing human infection
PlexinA3 restricts spinal exit points and branching of trunk motor nerves in embryonic zebrafish
Get PDFThe pioneering primary motor axons in the zebrafish trunk are guided by multiple cues along their pathways. Plexins are receptor components for semaphorins that influence motor axon growth and path finding. We cloned plexinA3 in zebrafish and localized plexinA3 mRNA in primary motor neurons during axon outgrowth. Antisense morpholino knock-down led to substantial errors in motor axon growth. Errors comprised aberrant branching of primary motor nerves as well as additional exit points of axons from the spinal cord. Excessively branched and supernumerary nerves were found in both ventral and dorsal pathways of motor axons. The trunk environment and several other types of axons, including trigeminal axons, were not detectably affected by plexinA3 knock-down. RNA overexpression rescued all morpholino effects. Synergistic effects of combined morpholino injections indicate interactions of plexinA3 with semaphorin3A homologs. Thus, plexinA3 is a crucial receptor for axon guidance cues in primary motor neurons
Axon–glial disruption: the link between vascular disease and Alzheimer's disease?
No full textVascular risk factors play a critical role in the development of cognitive decline and AD (Alzheimer's disease), during aging, and often result in chronic cerebral hypoperfusion. The neurobiological link between hypoperfusion and cognitive decline is not yet defined, but is proposed to involve damage to the brain's white matter. In a newly developed mouse model, hypoperfusion, in isolation, produces a slowly developing and diffuse damage to myelinated axons, which is widespread in the brain, and is associated with a selective impairment in working memory. Cerebral hypoperfusion, an early event in AD, has also been shown to be associated with white matter damage and notably an accumulation of amyloid. The present review highlights some of the published data linking white matter disruption to aging and AD as a result of vascular dysfunction. A model is proposed by which chronic cerebral hypoperfusion, as a result of vascular factors, results in both the generation and accumulation of amyloid and injury to white matter integrity, resulting in cognitive impairment. The generation of amyloid and accumulation in the vasculature may act to perpetuate further vascular dysfunction and accelerate white matter pathology, and as a consequence grey matter pathology and cognitive decline.</jats:p
Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells
Get PDFLow remyelination efficiency after spinal cord injury (SCI) is a major restraint to successful axonal and functional regeneration in mammals. In contrast, adult zebrafish can: (i) regenerate oligodendrocytes and myelin sheaths within 2 weeks post lesion; (ii) re-grow axonal projections across the lesion site and (iii) recover locomotor function within 6 weeks after spinal cord transection. However, little is known about the intrinsic properties of oligodendrocyte progenitor cells (OPCs), the remyelinating cells of the central nervous system (CNS). Here, we demonstrate that purified OPCs from the adult zebrafish spinal cord are electrically active. They functionally express voltage-gated K+ and Na+ channels, glutamate receptors and exhibit depolarizing, tetrodotoxin (TTX)-sensitive spikes, as previously seen in rodent and human OPCs. Furthermore, we show that the percentage of zebrafish OPCs exhibiting depolarizing spikes and Nav-mediated currents is lower as compared to rodent white matter OPCs, where these membrane characteristics have been shown to underlie OPC injury susceptibility. These findings imply that adult zebrafish OPCs resemble electrical properties found in mammals and represent a relevant cell type towards understanding the biology of the primary cells targeted in remyelination therapies for non-regenerative species. The in vitro platform introduced in this study could be used in the future to: (i) elucidate how membrane characteristics of zebrafish OPCs change upon injury and (ii) identify potential signaling components underlying OPC injury recognition
Optimizing the concrete load-bearing structure of high-rise buildings: Combining the ground structure method with a recursive resizing algorithm on a case study
No full textTheshortage in housing and office spaces, combined with the desire of people tolive in densely populated cities results in a lack of space. A proposedsolution can be found in the usage of high-rise buildings. Nowadays there ismore awareness for the environment, thus this research tries to reduce theenvironmental impact of a high-rise building by optimizing the material used inload-bearing structures. This research aims to give designers and engineersmore insight into the added value of structural optimization; in particular forthe material usage in the load-bearing structure of high-rise buildings. Theresearch objective is formulated as follows: What is the optimal topology for a reinforced concrete load-bearingstructure, situated at the perimeter of a high-rise building when optimizingthe material use? A building isclassified as high-rise building when its roof is 70 m or more above groundlevel and accommodates work and/or living space. In literature the distinctionis made between three sorts of optimization: size, shape and topologyoptimization. Topology optimization has the most freedom, therefore it is morelikely to find a novel structure which minimizes the material use as much aspossible. In specific the Ground Structure Method (GSM) is used. The initialground structure is constructed by creating members between all nodes (fullconnectivity) which are located in the design space. The cross-sectional areasof the bars are the design variables in the optimization. An option is that thevariables turn to zero, thus elements are deleted resulting in less material.The conventional GSM starts with the full connectivity as initial groundstructure, deletes elements and calculates the new load distribution until athreshold is reached. In the end the load-bearing structure will consist ofcolumns, braces and beams, which are located at the perimeter of the floorplan. Literature indicates that (high-rise) buildings which are mainly loadedby horizontal loads would be optimal if they contain arches in the load-bearingstructure, or resemble the so-called Michell truss. However, the influence ofthe vertical load is often not taken into account, therefore this isinvestigated with the help of a parametric study. To compare the results withreality, a case study (Boston & Seattle, Rotterdam) is investigated. The core of the research is the furtherdevelopment of the optimization code, based on the GSM, written by He et al.,where multiple load cases and demands for the material strengths are implemented.In contrast to deleting elements, the code uses an adaptive ‘member adding’scheme, which is firstly proposed by Gilbert and Tyas. This scheme solvesproblems faster than the conventional GSM, up to 8 times, and is able to solvelarge problems. This code is extended during this research with new functionswhich implement demands for fire, second-order, buildability, flexural bucklingand stiffness. Also it is possible to add self-weight to the optimization. Thestiffness is implemented by adding a constraint to the displacement of the topof the building, wherefore a recursive resizing algorithm is written based onthe article of Chan. Thus the extended code exists of two optimizations, firsta strength optimization and afterwards a stiffness optimization. The code iswritten in Python and for the purpose of post-processing exporting the data toExcel and Abaqus is possible. With thehelp of the extended code, two design spaces are investigated. One design spaceis smaller, such that the computational time is low and many variations can beexamined during the parametric study on the total vertical to total horizontalload ratio (v/h-ratio). The other is based on a case study for comparison witha realistic situation. The verification of the code shows that the extrafunctions work properly for the investigated problems. If the functionsconcerning the stiffness optimization, inclusion of self-weight, fire,buildability and second-order are used, the extended code becomes unstable andthe computation time increases enormously when optimizing the case study.Therefore it is chosen not to include during obtaining the results. The resultsof the parametric study shows an expected pattern for the load-bearingstructure, for a v/h-ratio below 4. The pattern consist of arches, originatingfrom the Michell truss. The optimization of the case study, subjected to onerealistic load combination, showed no clear pattern for the load-bearingstructure. Post-processing steps, based on engineering judgement, are taken toclarify the solutions, which showed that the columns above the supports shouldbe large in comparison to the other elements and that the arches are the mostoptimal structure. Therefore an “optimized” load-bearing structure consistingof arches is proposed. The analysis of the “optimized” load-bearing structureshows us that most of the elements meet the strength requirements. To find theoptimal solution an iterative process would be needed, because increasing thecross-section of an element will decrease the stress but increase thestiffness, thus attracting more load. Thedifference between the optimized load-bearing structure and the originalload-bearing structure of the case study is that the optimized uses archesinstead of (punched) structural walls and uses less material (±3%). From thepost-processing of the results it is concluded that increasing the strengthratio (compression to tensile) to 1.0, decreasing the total v/h-ratio orignoring the rigid-diaphragm working of the floor help clarify the results ofthe optimization. This research extendsthe current literature with extra insights in the use of the Ground StructureMethod in an optimization code. Also, it confirms that the arches (originatingfrom Michel Truss) is an efficient manner to transfer the loads to thesupports. However, more research in the influence of the supports, the designspace and the material type on the clearness of the optimal solution is needed. The advice for designers and engineersis to see what the possibilities are for arches to use in their load-bearingstructure, because these are efficient in transferring the loads so materialcan be saved. The current version of the code needs to be made more userfriendly, stabler and faster before it is recommended to be used by designersand engineers. The extended code is a first attempt to implement multiple rulesfrom the Eurocode in a optimization code. Related dataset 4TU.ResearchData https://doi.org/10.4121/uuid:d5a42352-a417-4082-aa0f-87047812bfedCivil Engineering | Structural Engineering | Structural Mechanic
Advances in ITP - therapy and quality of life - a patient survey.
Get PDFCurrent guidelines recommend glucocorticoids and splenectomy as standard 1(st) and 2(nd) line treatments for chronic immune thrombocytopenia (ITP). We sought to find out how German ITP-patients are treated with respect to these guidelines. Members of a patient support association ≥18 years with a self-reported history of chronic ITP>12 months were surveyed with a web-based questionnaire. 122 questionnaires were evaluated. 70% of patients had chronic ITP for more than 5 years and 20% an average platelet count of ≤30·10(9)/L. 41% of the patients reported haematomas or petechiae more than once or twice and up to 12 times or more per year and 17% oropharyngeal and nasal bleeds. 11% had been admitted to hospital during the last 12 months. 88% had received or currently receive glucocorticoids, 27% were splenectomised. IVIG had been given to 55%, rituximab to 22%, anti-D to 12%, ciclosporin to 7%, while complementary and alternative medical treatments had been used by 36%. 50 women responded to questions concerning pregnancy. 14 (28%) had been advised not to become pregnant. 23 reported pregnancies and 10 (44%) required treatment for their ITP during pregnancy. Glucocorticoids are the most common therapy for chronic ITP but complementary and alternative treatments already come second and less than ⅓ of patients are splenectomised. This and the frequent use of complementary medicines suggests patients' dissatisfaction with conventional approaches. Many patients receive off-label therapies. There is a major need for adequate counselling and care for pregnant ITP-patients
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