1,721,007 research outputs found
Molecular Analyses Of Gypb In African Brazilians
No full textThe molecular background of variant forms of GYPB is not well studied in Brazilians of African descent. The present study was carried out to determine the molecular bases of the S-s- phenotype and the frequency of GYPB*S silent gene for the S-s+ phenotype in a blood donor population of African Brazilians. In this study, 165 blood samples from African Brazilians (Northeastern Brazil) who phenotyped as S-s- (n = 17) and S-s+ (n = 148) by hemagglutination were selected. Allele-specific (AS)-PCR and PCR-restriction fragment length polymorphism (RFLP) were used to identify the variant forms of GYPB. In 13 of 17 S-s- samples (76.5%), both GYPB were deleted. In 137 of the 148 S-s+ samples (92.6%), the AS-PCR was consistent with the S-s+ phenotype. In 4 of the S-s- samples (23.5%) and 11 of the S-s+ samples (7.4%), the AS-PCR showed the presence of a GYPB*S allele associated with silencing of S. In the 4 donors with the S-s- phenotype, there was homozygosity (or hemizygosity) for the GYP(P2) allele (n = 2), homozygosity (or hemizygosity) for the GYP(NY) allele (n = 1), and heterozygosity for the GYP(P2) and GYP(NY) alleles (n = 1). In the 11 donors with the S-s+ phenotype, there was heterozygosity for GYP(P2) allele (n = S) and heterozygosity for GYP(NY) allele (n = 3). This study reports for the first time the molecular mechanisms responsible for the S-s- phenotype in a population of African Brazilians and provides new information about the frequency and molecular bases of the GYPB*S silent gene (7.4%) in this population.244148153Daniels, G.L., Fletcher, A., Garratty, G., Blood group terminology 2004: From the International Society of Blood Transfusion committee on terminology for red cell surface antigens (2004) Vox Sang, 87, pp. 304-316Reid, M.E., Lomas-Francis, C., (2004) Blood group antigen factsbook, , 2nd ed. San Diego: Academic PressBlumenfeld, O.O., Huang, C.-H., Molecular genetics of the glycophorin gene family. The antigens for MNSs blood groupsmultiple gene rearrangements and modulation of splice site usage result in extensive diversification (1995) Hum Mutat, 6, pp. 199-209Storry, J.R., Reid, M.E., Fetics, S., Huang, C.-H., Mutations in GYPB exon 5 drive the S-s-U+var phenotype in persons of African descent: Implications for transfusion (2003) Transfusion, 43, pp. 1738-1747Storry, J.R., Reid, M.E., MacLennan, S., Lubenko, A., Nortman, P., The low-incidence MNS antigens Mv, sD, and Mit arise from single amino acid substitutions on GPB (2001) Transfusion, 41, pp. 269-275Huang, C.-H., Johe, K., Moulds, J.J., Siebert, P.D., Fukuda, M., Blumenfeld, O.O., Glycophorin (Glycophorin B) gene deletion in two individuals homozygous for the S-s-U- blood group phenotype (1987) Blood, 70, pp. 1830-1835. , ¶Huang, C.-H., Reid, M.E., Blumenfeld, O.O., Remodeling of the transmembrane segment in human glycophorin by aberrant RNA splicing (1994) J Biol Chem, 269, pp. 10804-10812Huang, C.-H., Blumenfeld, O.O., Reid, M.E., Alternative splicing of a novel glycophorin allele GPHe(GL) generates two protein isoforms in the human erythrocyte membrane (1997) Blood, 90, pp. 391-397Reid, M.E., Storry, J.R., Ralph, H., Expression and quantitative variation of the low incidence blood group antigen He on some S-s- RBCs (1996) Transfusion, 36, pp. 719-724Rosse, W.F., Gallagher, D., Kinney, T.R., Transfusion and alloimmunization in sickle cell disease. The cooperative study of sickle cell disease (1990) Blood, 76, pp. 1431-1437Dhandsa, N., Williams, M., Joss, V., Patten, J., James, D., Sinclair, L., Haemolytic disease of the newborn caused by anti-U (1981) Lancet, 2, p. 1232Gottschall, J.L., Hemolytic disease of the newborn with anti-U (1981) Transfusion, 21, pp. 230-232Smith, G., Knott, P., Rissik, J., Win, N., Anti-U and haemolytic disease of the fetus and newborn (1998) Br J Obstet Gynaecol, 105, pp. 1318-1321Reid, M.E., Storry, J.R., Maurer, J., Nance, S.T., Practical method for determination of the U status of S-s- erythrocytes (1997) Immunohematology, 13, pp. 111-114Kan, Y.W., The William Allan Memorial Award Address: Thalassemia: molecular mechanism and detection (1986) Am J Hum Genet, 38, pp. 4-12Lowe, R.F., Moores, P., S-s-U- red cell factor in Africans of Rhodesia, Malawi, Mozambique and Natal (1972) Hum Hered, 22, pp. 344-350Habibi, B., Fouillade, M.T., Duedari, N., The antigen Duclos. A new high frequency red cell antigen to Rh and U (1978) Vox Sang, 34, pp. 302-309Dahr, W., Moulds, J.J., High-frequency antigens of human erythrocyte membrane sialoglycoproteins, IV. Molecular properties of the U antigen (1987) Biol Chem Hoppe-Seyler, 368, pp. 659-66
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Prevalence Of Rhd*dol And Rhce*ce(818t) In Two Populations
No full textThe alleles RHCE*ceBI (RHCE*ce 48C, 712G, 818T, 1132G) and RHCE*ceSM (RHCE*ce 48C, 712G, 818T) encode the low-prevalence Rh antigen STEM. These alleles frequently travel in cis with RHD*DOL. To estimate the frequency of these alleles, we tested a total of more than 700 samples in two populations. Blood samples were obtained from patients with sickle cell disease and from blood donors of African descent. DNA extractions and analyses were performed by standard methods. In the United States, none of 70 patient samples had the RHCE*818 nucleotide change. Two of 220 donors (frequency of 0.009) were heterozygous for RHCE*818C/T (RHCE*ceBI). One of these samples had RHD/RHD*DOL and the other had RHD/RHD*DOL-2. In these 290 samples, no other RHD*DOL alleles were found. In Brazil, 1 of 244 patients with sickle cell disease (frequency of 0.004) and 1 of 171 donors (frequency of 0.006) were heterozygous for RHCE*818C/T (RHCE*ceBI). Testing of more than 500 additional samples from people of African descent, selected because they had a diverse range of common and variant RHCE alleles, did not reveal a sample with RHD*DOL or RHD/RHD*DOL-2 in the absence of RHCE*ce(818T). Although the numbers are small, our study shows that in the United States, the frequency of RHCE*818T is 0.007 (2 in 290 samples) and in Brazil it is 0.004 (2 in 515 samples). The four RHCE*818T alleles were RHCE*ceBI. © 2011 by The American National Red Cross.2726667Marais, I., Moores, P., Smart, E., Martell, R., STEM, a new low-frequency Rh antigen associated with the e-variant phenotypes (1993) Transfus Med, 3, pp. 35-41. , hr S -(Rh: -18, -19) and hr B -(Rh: -31-34)Halter-Hipsky, C., Hue-Roye, K., Coghlan, G., Lomas-Francis, C., Reid, M.E., Two alleles with RHCE*nt818C>T change encode the low prevalence Rh antigen STEM (2009) Blood, 114 (SUPPL.), pp. 1226-1227. , [abstract]Halter Hipsky, C., Hue-Roye, K., Lomas-Francis, C., Reid, M.E., RHD*DOL travels with RHCE*ce(818C>T), which encodes a partial e, hr S -, hr B + STEM+ phenotype (2010) Transfusion, 50 (SUPPL.), pp. 48A. , [abstract]Halter Hipsky, C., Lomas-Francis, C., Fuchisawa, A., RHCE*ceCF encodes partial c and partial e but not CELO, an antigen antithetical to Crawford (2011) Transfusion, 51, pp. 25-3
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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