6 research outputs found
マスト細胞における琥珀抽出物の抗アレルギー作用に関する研究
No full text筑波大学University of Tsukuba博士（理学）Doctor of Philosophy in Science2023doctoral thesi
マスト細胞における琥珀抽出物の抗アレルギー作用に関する研究
No full text筑波大学University of Tsukuba博士（理学）Doctor of Philosophy in Science2023【要旨】thesi
Potensi Ekstrak Daun Tin (Ficus carica L.) sebagai Antioksidan dan Aktivitas Hambatannya terhadap Proliferasi Sel Kanker HeLa
No full textFig fruit (Ficus carica L.) has antioxidant dan anticancer properties both empirically and scientific reseach. However, scientific research on the use of fig leaf as anticancer has not reported yet. This study examined the antioxidant and anticancer protency of fig leaf extract. Based on the phytochemicals test, simplicia of fig leaf contains flavonoids, tannins, steroids and alkaloids. Flavonoids, tannins, steroids of fig leaf were extracted by maceration technique using suitable solvent. The extracts were tested by 1,1-diphenyl-2-picrylhydrazyl antioxidant method and brine shrimp lethality test toxicity method. Flavonoid extract showed highest antioxidant activity with IC50 150 mg/L, while the LC50 for toxicity tests was 191.43 ppm. Flavonoids extract of fig leaf was fractionated using preparative thin layer chromatography and obtained seven fractions.Proliferation inhibition test against HeLa cancer cell by 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide method showed the most active fraction was fraction F7 which inhibit 57.18 % proliferation of HeLa cancer cells at concentrations of 800 ppm. Based on the identification using ultraviolet spectrophotometer and fourier transform infrared, F7 fraction suspected to contain isoflavones or flavones compounds
Amber Extract Suppressed Mast Cell-Mediated Allergic Inflammation via the Regulation of Allergic Mediators—An In Vitro Study
No full textThe various clinical approaches for treating allergy-related diseases have shown modest progress in low side effects and improved clinical outcomes. Therefore, finding alternative anti-allergic agents is crucial. The present study explored the anti-allergic effects of amber extract (fossilized tree resin) in RBL-2H3 mast cells stimulated with different allergens. In order to support the information on the inflammatory effect of the amber extract, NO production analysis on RAW 264.7 cells was conducted. β-Hexosaminidase release, an indicator of the efficacy of the amber extract in preventing mast cell activation and degranulation, reactive oxygen species (ROS) generation, and the effect of the amber extract on key cytokines production on RBL-2H3 cells, was evaluated. The results demonstrated that amber extract at concentrations up to 50 μg/mL had no cytotoxic effect on RAW 264.7 and RBL-2H3 cells. Amber extract inhibited NO production in RAW 264.7 cells. Treatment with amber extract significantly suppressed the release of β-hexosaminidase, especially at 50 μg/mL. Furthermore, amber extract suppressed the significantly increased ROS levels induced by allergen stimulation and allergy-associated cytokines. The results also suggested that amber extract exerts anti-allergic inflammatory effects by inhibiting the MAPK and NF-κB signaling pathways, resulting in decreased cytokines production. Thus, the amber extract is a promising anti-allergic agent
Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia
No full textFermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food
