3,383 research outputs found
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Determinação das perdas suplementares em motores de indução trifásicos pelo método EH-STAR
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Elétrica.Este trabalho se refere ao estudo e a implementação do método de ensaio eh-star para determinação das perdas suplementares em motores de indução trifásicos, proposto na norma IEC 60034-2-1. O ensaio eh-star é realizado sem a necessidade de acoplar o motor a um dinamômetro ou máquina auxiliar, ou seja, o motor trabalha em vazio. Durante o teste o motor é conectado em estrela, com um resistor, denominado Reh, ligado entre dois terminais de alimentação do motor, sendo o mesmo ligado a uma fonte monofásica. Como resultado aparecerá em seus terminais um sistema trifásico desequilibrado (assimétrico), que será decomposto em dois sistemas simétricos: seqüência positiva e seqüência negativa. São apresentados resultados de diversos motores testados na WEG. Também é apresentada uma simulação deste ensaio com o programa de cálculo utilizado pela WEG Motores. O presente trabalho propõe ainda um novo circuito para obtenção desta alimentação desequilibrada, ligando os terminais do motor à duas fases e o neutro de um gerador trifásico, dispensando a necessidade da utilização do resistor Reh. This work mentions the study and the implementation regarding the eh-star test method proposed in the IEC Standard 60034-2-1 for the determination of stray load losses in three-phase induction motors. This test is accomplished with no load, that is, the motor does not need to be connected to a dynamometer or an auxiliary machine. During the test, the motor is star connected with a resistor, referred to as Reh, installed between two motor terminals. The motor is fed by a single-phase voltage supply. This will result in an unbalanced (asymmetric) three-phase system at the motor's terminals, which can be decomposed into two symmetric systems: positive sequence and negative sequence. Results of several tests accomplished in WEG facilities, as well as simulation results obtained with the software used in WEG for motor calculation, are also presented. Additionaly, the present work proposes a new circuit for the attainment of this unbalanced voltage supply, using two phases and the neutral of a three-phase generator, avoiding the need for the Reh resistor
Red Organic Light-Emitting-Diodes based on a N-Annulated Perylene Diimide Dimer
In this contribution we report on solution
processed red OLEDs based upon a N-annulated perylene diimide dimer, namely
tPDI2N-EH, a red-light emitting molecule. OLED devices with the
architecture of glass/ITO/PEDOT:PSS/EML/LiF/Ag (EML = emitting layer) were
fabricated with EMLs comprised of tPDI2N-EH neat and blended with
poly (9,9-dicotylfluorene, PFO), all solution processed from non-halogenated
solvents. The photophysical and electrophysical performance of PFO:tPDI2N-EH-blend
films with different composition ratios were investigated. The PFO:tPDI2N-EH-based
OLEDs with a 2:18 ratio exhibited best performance. The PFO:tPDI2N-EH-based
OLEDs gave red electroluminescence with the emission wavelength of 635 nm and
the CIE (international commission on illumination) coordinates of (x = 0.672, y
= 0.321). OLEDs with EMLs fabricated using roll-to-roll compatible methods are
also demonstrated.</p
Avaliação in vitro do efeito da desproteinização da dentina decídua de humanos na união de sistemas adesivos
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Odontologia, Florianópolis, 2009.O objetivo deste estudo, in vitro, foi avaliar a resistência de união à microtração e a nanoinfiltração, após desproteinização da dentina decídua humana. Foram utilizadas coroas de molares decíduos hígidos, as quais tiveram a superfície oclusal desgastada com lixas de carbeto de silício, até a completa exposição da superfície dentinária, e para padronização da smear layer. Os espécimes foram distribuídos em seis grupos, de acordo com o tipo de tratamento (condicionamento ácido - CA ou CA + hipoclorito de sódio - NaOCl) e os sistemas adesivos: One Step Plus - Bisco (OSP), Single Bond - 3M ESPE (SB), Prime & Bond 2.1 - Dentsply (PB). Para o procedimento de desproteinização foi utilizado hipoclorito de sódio 10% por 30s. Os sistemas adesivos e a resina (Filtek Z 250 - 3M ESPE) foram aplicados de acordo com as recomendações dos fabricantes e os espécimes armazenados em água destilada (37ºC/24h). As coroas foram seccionadas obtendo-se palitos (0,8mm2), os quais foram imediatamente submetidos ao teste de resistência à microtração (Instron - 0,5mm/min), até fratura dos corpos-de-prova. Os valores obtidos foram analisados por ANOVA e teste de Tukey (p<0,05). Os corpos-de-prova foram levados ao microscópio eletrônico de varredura (MEV), para visualização do tipo de fratura e os dados submetidos ao teste de Kruskal-Wallis (p<0,05).A nanoinfiltração foi avaliada utilizando-se palitos e nitrato de prata amoniacal como marcador químico. A deposição da prata foi visualizada ao MEV e analisada de duas formas: 1. Em porcentagem (%), em três regiões do palito, utilizando-se espectometria por energia dispersa por raio-x (EDS); 2. Atribuição de escores pela avaliação das fotomicrografias obtidas ao MEV. Os dados (%) foram analisados por ANOVA e teste de Tukey (p<0,05) e os escores submetidos aos testes de Kruskal-Wallis e U de Mann-Whitney (p<0,05).Os valores médios obtidos para o teste de microtração sem desproteinização foram [MPa(DP)]: PB - 35,95(6,12); SB - 28,82(6,38); OSP - 24,59(6,10); e após desproteinização: PB - 41,47(6,79); OSP - 31,09(9,16); SB - 25,55(7,23). Os padrões de fratura mais comumente encontrados foram coesiva do adesivo e mista, para todos os grupos. A nanoinfiltração, avaliada por porcentagem, apresentou diferença significante para as variáveis tratamento e adesivo. A desproteinização da dentina condicionada reduziu significativamente a infiltração para o adesivo OSP. Para a variável sistema adesivo, na análise por porcentagem e por escores, o adesivo SB apresentou significativamente maior infiltração pelo nitrato de prata quando comparado ao OSP e PB, que foram similares entre si. Conclui-se que a resistência adesiva não foi influenciada pela remoção do colágeno exposto pelo condicionamento ácido e que a nanoinfiltração não foi evitada pela desproteinização dentinária
α<sub>5</sub>β<sub>1</sub> integrin is activated by <i>Eh</i> contact via a surface-bound-integrin-binding <i>Eh</i> cysteine protease.
(a) Localization of α5β1 integrin (green) and phosphorylated (active)-paxillin (red) in PMA-differentiated macrophages at sites of Eh contact; mammalian nuclei, blue (Eh nuclei are not labeled). (b) Diagram showing structural organization of EhCP5. (c) Subcellular distribution of EhCP5 (red) and α5β1 integrin (green) after THP-1 macrophages were cultured with rCP5 (left) or rCP5 in which the RGD site was mutated to RAD (right); nuclei, blue. (d-f) Immunoblot analysis of β1 integrin tyrosine phosphorylation (clone PY20) of anti-α5β1 integrin immunoprecipitates from PMA-differentiated THP-1 macrophages stimulated with Wt Eh (d-f), with PP1 or PP3 control (d), or blocking peptide RGDSP or RADSP control (e) added to cultures 10 min before stimulation, or EhCP5- Eh (f). (g-i) Activation status of β1 integrin in PMA-differentiated THP-1 macrophages stimulated with Wt (g-i), or EhCP5- Eh (i) evaluated with anti-β1 integrin mAbs, AG89 (g-i) and HUTS-4 (i) that recognize the active conformation specific epitope of β1 integrin. Scale bars, 10 μM. Data are representative of two (a, c, i) or three (d-h) separate experiments.</p
Active sites comparison of functional ODC, antizyme inhibitor and <i>Eh</i>ODC.
<p><b>A</b>) Human ODC active site residues colored in blue. <b>B</b>) <i>Eh</i>ODC active site residues identical to human ODC colored blue, residues identical to AZI colored green and unique to <i>Eh</i>ODC colored red. <b>C</b>) AZI interface region showing residues identical to human ODC in blue and those are mutated colored green.</p
The effect of the CK and EH on the motility and force exerted by filopodia.
<p><b>(a-b)</b> Phase contrast images of GC before and after treatment with 20 μM EH. Note the length of filopodia in each case. Scale bar 5 μm. <b>(c-d)</b> Staining of F-actin by phalloidin in GC before and after treatment with 20 μM EH. <b>(e)</b> Rate of filopodia protrusion in control conditions (red), with 25 μM CK (green), with 50 μM CK (dark green), with 10 μM EH (cyan) and with 20 μM EH (blue). <b>(f)</b> Maximum length of filopodia in control conditions (red), with 25 μM CK(green), with 50 μM CK(dark green), with 10 μM EH (cyan) and with 20 μM EH (blue). <b>(g)</b> Images of a bead trapped in front of a filopodium emerging from a GC of DRG neuron in the presence of 25 μM CK. At t<sub>1</sub> the bead is in the optical trap and at t<sub>2</sub> the filopodium pushes the bead. The cross indicates the centre of the optical trap. (<b>h)</b> The three components F<sub>x</sub>, F<sub>y</sub> and F<sub>z</sub> of the force exerted by the filopodium in the presence of 25 μM CK. <b>(i-k)</b> As in (h) but in the presence of 50 μM CK (i), in the presence of 10 μM EH (j) and in the presence of 20 μM EH (k) respectively. <b>(l)</b> Filopodia force in control conditions (red), in the presence of 25 μM CK (green), of 50 μM CK (dark green), of 10 μM EH (cyne) and of 20 μM EH (blue). The trap stiffness was k<sub>x,y</sub> = 0.10 pN/nm, k<sub>z</sub> = 0.08 pN/nm. By using the student t-test, the data differs with respect to the control conditions with a significance of *P<0.05 and **P<0.005. Data represent mean ± SEM. All the data were checked with chi-square test for Normal distribution before applying the student’s t test.</p
Cluster AZ and EH phages share gene content in excess of <i>Microbacterium</i> clustering parameters.
GCS values were recorded using the PhagesDB Explore Gene Content tool and visualized as a heat map using Prism 8.0.0. Cells boxed in white represent pairwise GCS values in excess of gene content clustering parameters (≥35%) between phages belonging to different clusters. Cells boxed in black represent phage clusters. The former singleton Zeta1847 is indicated in red. “Str.” indicates phages infecting Streptomyces. Some phages in Clusters AZ and EH shared over 35% GCS, in excess of the Microbacterium clustering parameter. The former singleton Zeta1847 shared over 35% GCS with Cluster EH phages, which resulted in the clustering of this phage with Cluster EH.</p
The effect of CK and EH on the force generated by lamellipodia.
<p><b>(a)</b> Low-resolution image of a bead trapped in front of a lamellipodium emerging from the soma of a DRG neuron in the presence of 25 μM CK (25 μM CK). Scale bar, 5μm. (<b>b-c</b>) High-resolution images during a push. At t<sub>1</sub> the bead is in the optical trap (b) and when the lamellipodium grows, at t<sub>2</sub>, it pushes the bead (c). The red cross indicates the centre of the optical trap. Scale bar, 2μm. (<b>d</b>) The three components F<sub>x</sub>, F<sub>y</sub>, and F<sub>z</sub> of the force exerted when the lamellipodium pushes the bead. (<b>e</b>) As in (d) but in the presence of 50 μM CK (CK 50 μM). (<b>f</b>) As in (d) but in the presence of 10 μM EH (EH 10 μM). (<b>g</b>) As in (d) but in the presence of 20 μM EH (EH 20 μM). The trap stiffness is k<sub>x,y</sub> = 0.10, k<sub>z</sub> = 0.08 pN/nm. <b>(h)</b> Comparison of the force exerted by lamellipodia in control conditions (red), with 25 μM CK (green), with 50 μM CK (dark green), with 10 μM EH (cyan) and with 20 μM EH (blue) and in all the four different stereotyped behaviours: LP, LR, VP and VR. In each case, by using the student t-test, the force measured in the presence of each inhibitor was lower than that measured in control conditions with a significance *P<0.005. Data represent mean ± SEM.</p
Red Organic Light-Emitting-Diodes based on a N-Annulated Perylene Diimide Dimer
In this contribution we report on solution
processed red OLEDs based upon a N-annulated perylene diimide dimer, namely
tPDI2N-EH, a red-light emitting molecule. OLED devices with the
architecture of glass/ITO/PEDOT:PSS/EML/LiF/Ag (EML = emitting layer) were
fabricated with EMLs comprised of tPDI2N-EH neat and blended with
poly (9,9-dicotylfluorene, PFO), all solution processed from non-halogenated
solvents. The photophysical and electrophysical performance of PFO:tPDI2N-EH-blend
films with different composition ratios were investigated. The PFO:tPDI2N-EH-based
OLEDs with a 2:18 ratio exhibited best performance. The PFO:tPDI2N-EH-based
OLEDs gave red electroluminescence with the emission wavelength of 635 nm and
the CIE (international commission on illumination) coordinates of (x = 0.672, y
= 0.321). OLEDs with EMLs fabricated using roll-to-roll compatible methods are
also demonstrated.</p
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