197,331 research outputs found
Xavier Reboud invité de l'émission « Le magazine de la santé », interview par M. Carrère d'Encausse
No full textXavier Reboud invité de l'émission « Le magazine de la santé », interview par M. Carrère d'Encauss
INHIBITION OF PROTEASOME ACTIVITY PROMOTES THE CORRECT LOCALIZATION OF DESEASE-CAUSING ALPHA-SARCOGLYCAN MUTANTS IN A HETEROLOGOUS CELL SYSTEM EXPRESSING BETA-, GAMMA-, AND DELTA-SARCOGLYCAN
No full textSarcoglycanopathies are progressive muscle wasting disorders caused by genetic defects of four proteins, alpha-, beta-, gamma-, and delta-sarcoglycan, elements of a key transmembrane complex of striated muscle. The proper assembly of the sarcoglycan complex represents a critical issue of sarcoglycanopathies, as several mutations severely perturb tetramer formation. Misfolded proteins are generally discarded through the cell’s quality-control system that, however, could lead to the removal of functional polypeptides. To explore whether it is possible to rescue sarcoglycan mutants by preventing their degradation, we generated a heterologous cell system constitutively expressing three (beta, gamma, and delta) of the four sarcoglycans. In these cells (bgd-HEK), the lack of alpha-sarcoglycan prevented complex formation and cell surface localization, while the presence of alpha-sarcoglycan allowed maturation and targeting of the tetramer. On the contrary, transfection of bgd-HEK cells with disease-causing alpha-sarcoglycan mutants led to a dramatic reduction of mutated proteins, compared to wild type, and the absence of the complex from cell surface. This result closely reproduces the observations made in muscle patients. Proteasomal inhibition reduced the degradation of mutants and facilitated the assembly and targeting of the sarcoglycan complex to the plasma membrane. However, as expected for a critical process necessary for cell survival, the prolonged inhibition of proteasome severely compromised cell viability. Importantly, when the treatment included the inhibition of caspase activity, the cytotoxic effect of proteasome inhibitor was almost completely abolished. Finally, in a trial application of this approach to a skeletal muscle explant isolated from an LGMD-2D patient, incubation with the FDA-approved proteasome inhibitor Velcade (bortezomib) rescued the expression of mutant alpha-sarcoglycan to the cell membrane. The present data provide important insights for the development of pharmacological therapies for sarcoglycanopathies. Funded by AFM (grant # 12988) and University of Padova
Toward the First Nonpeptidic Molecular Tong Inhibitor of Wild-Type and Mutated HIV-1 Protease Dimerization
No full textSOUFFLE DE VIE : QUATORZE CANTIQUES POUR L'ANNEE LITURGIQUE / LA CHORALE ALLELUIA ; dir : ABBE REBOUD
No full textComprend : L'ANGE DE DIEU" Vieux choral alsacien / M. FUSTIER, J. BONFILS - O JESUS, QUAND TU T'ABAISSES / P. LOROGIOLA, GOUDIMEL - SOUFFLE DE VIE / J. VERGRIETE, D. JULIEN, R. REBOUD - MYSTERE DU CALVAIRE / ROZIER, D. RIMAUD, J.S. BACH - JE CROIS EN DIEU / CL. ROZIER D. RIMAUD, GOUDIMEL, M. ROSE - SEIGNEUR, JE N'OSE PAS VOUS DIRE / P. PELOT, NEUMARK, J.S. BACH - JE REGARDE VERS LES MONTAGNES / Texte et musique de R. REBOUD - TERRE ENTIERE / J. LECLERCQ, L. DEISS, R. REBOUD - NOUS T'OFFRONS CE PAIN / L. BARJON, J.S. BACH - CHANT DE MARIAGE / J.C. RENARD, R. REBOUD - JOYEUSE LUMIERE / Texte et musique de P. LUCIEN DEISS - VOUS PRENEZ, SEIGNEUR, L'APPARENCE / P. GEGHRE, J.S. BACH - UN PETIT ENFANT EST NE : Mélodie anglaise / Texte et harmonisation de R. REBOUD - HOSANNA, LA SAINTE CROIX / P. DONCOEURBnF-Partenariats, Collection sonore - BelieveContient une table des matière
Comparison of heat-shock responses between the hydrothermal vent shrimp Rimicaris exoculata and the related coastal shrimp Palaemonetes varians
No full textThe deep-sea vent shrimp Rimicaris exoculata is believed to occur at the hot end of the hydrothermal biotope in order to provide essential elements to its epibiosis. Because it is found close to hot venting water, R. exoculata lives in a highly fluctuating environment where temperature (2–40 °C in the swarms) can exceed its critical maximal temperature (33–38.5 ± 2 °C). In order to understand how this vent shrimp copes with hyperthermia, we compared its molecular heat stress response following an acute but non-lethal heat-shock (1 h at 30 °C) with that of its monophyletic shallow-water relative, the shrimp Palaemonetes varians, known to frequently undergo prolonged exposure at temperatures up to 30 °C in its natural environment during summer. We isolated four isoforms of heat-shock proteins 70 (HSP70) in R. exoculata (2 constitutive and 2 inducible isoforms) and two isoforms in P. varians (1 constitutive and 1 inducible isoform) and quantitatively compared their magnitude of induction at mRNA level, using real-time PCR, in the case of experimentally heat-stressed shrimps, with respect to control (unstressed) animals. Here, we report the first quantification of the expression of multiple hsp70 genes following heat stress in a deep-sea vent species living at 2300 m depth. Our results show a strong increase of hsp70 inducible genes in the vent shrimp (not, vert, similar 400-fold) compared to the coastal shrimp (not, vert, similar 15-fold). We therefore propose that, the highly inducible molecular response observed in R. exoculata may contribute to the ability of this species to tolerate thermal extremes
Adaptation to thermally variable environments: capacity for acclimation of thermal limit and heat shock response in the shrimp Palaemonetes varians
No full textIn the context of climate change, there is a sustained interest in understanding better the functional mechanisms by which marine ectotherms maintain their physiological scope and define their ability to cope with thermal changes in their environment. Here, we present evidence that the variable shrimp Palaemonetes varians shows genuine acclimation capacities of both the thermal limit (CTmax) and the heat shock response (hsp70 induction temperature). During cold acclimation to 10°C, the time lag to retune the stress gene expression to the current environmental temperature proved to exceed one week, thereby highlighting the importance of long term experiments in evaluating the species’ acclimation capacities. Cold and warm-acclimated specimens of P. varians can mobilise the heat shock response (HSR) at temperatures above those experienced in nature, which suggests that the species is potentially capable of expanding its upper thermal range. The shrimp also survived acute heat shock well above its thermal limit without subsequent induction of the HSR, which is discussed with regard to thermal adaptations required for life in highly variable environments
Supplemental Material - Awake extracorporeal life support and physiotherapy in adult patients: A systematic review of the literature
No full textSupplementary Material for Awake extracorporeal life support and physiotherapy in adult patients: A systematic review of the literature by Marta Cucchi, Silvia Mariani, Maria E De Piero, Justine M Ravaux, Michal J Kawczynski, Michele Di Mauro, Emma Shkurka, Aparna Hoskote and Roberto Lorusso in Perfusion.</p
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
CCDC 102126: Experimental Crystal Structure Determination
No full textRelated Article: F.Lebon, M.Ledecq, Z.Benatallah, S.Sicsic, R.Lapouyade, O.Kahn, A.Garcon, M.Reboud-Ravaux, F.Durant|1999|J.Chem.Soc.,Perkin Trans.2||795|doi:10.1039/a809270b,An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
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