1,720,981 research outputs found
Bacterial population in traditional sourdough evaluated by molecular methods
No full textAims: To study the microbial communities in artisanal sourdoughs, manufactured by traditional procedure in different areas of Sicily, and to evaluate the lactic acid bacteria (LAB) population by classical and culture-independent approaches. Methods and Results: Forty-five LAB isolates were identified both by phenotypic and molecular methods. The restriction fragment length polymorphism and 16S ribosomal DNA gene sequencing gave evidence of a variety of species with the dominance of Lactobacillus sanfranciscensis and Lactobacillus pentosus, in all sourdoughs tested. Culture-independent method, such as denaturing gradient gel electrophoresis (DGGE) of the V6¿V8 regions of the 16S rDNA, was applied for microbial community fingerprint. The DGGE profiles revealed the dominance of L. sanfranciscensis species. In addition, Lactobacillus-specific primers were used to amplify the V1¿V3 regions of the 16S rDNA. DGGE profiles flourished the dominance of L. sanfranciscensis and Lactobacillus fermentum in the traditional sourdoughs, and revealed that the closely related species Lactobacillus kimchii and Lactobacillus alimentarius were not discriminated
Microbial Consortia Involved in Traditional Sicilian Sourdough: Characterization of Lactic Acid Bacteria and Yeast Populations
Full text linkMetabolomic and Transcriptional Profiling of Oleuropein Bioconversion into Hydroxytyrosol during Table Olive Fermentation by Lactiplantibacillus plantarum
Full text linkRagusana donkey milk as a source of lactic acid bacteria and yeast strains of dairy technological interest
No full textDonkey milk is considered an interesting substitute to cow’s milk thanks to its nutritional properties. The chemical composition
of donkey milk has been extensively considered essential to prevent the growth of undesirable microorganisms, and
few data are available on its microbiological characterization.
In the present study raw and cultured donkey milk samples were analyzed in order to characterize lactic acid bacteria and
yeast populations. Both lactic acid bacteria and yeast isolated strains were identified using a combination of different molecular
techniques and results were confirmed by sequence analysis of the 16S rRNA gene and ITS regions, respectively.
Furthermore, technological traits of the strains were investigated.
The results indicated that dominant lactic acid bacteria strains were identified mainly as Lactococcus lactis subsp. lactis/cremoris
and Lactobacillus paracasei. The strains showed both good coagulant and acidifying activities and 50% of them was able to
grow in presence of 5.0 g/l of lysozyme. Among isolated yeast strains, the 70% was ascribed to the Kluyveromyces lactis species.
The results of present study indicated that Ragusana donkey milk is an interesting source of lactic acid bacteria and yeast
strains of technological interest
Characterization of Cell-Envelope Proteinases from Two Lacticaseibacillus casei Strains Isolated from Parmigiano Reggiano Cheese
Full text linkPRELIMINARY CHARACTERIZATION OF WILD LACTIC ACID BACTERIA AND THEIR ABILITIES TO PRODUCE FLAVOUR COMPOUNDS IN RIPENED MODEL CHEESE SYSTEM.
No full textSurvival of Lactobacillus rhamnosus probiotic strains in peach jam during storage at different temperatures
Full text linkSurvival of six probiotic wild strains of Lactobacillus rhamnosus, in comparison with a type strain, into peach synthetic medium (PSM) and commercial peach jam (PJ), was followed during 78 days of storage at 25 and 5°C. Changes in viable cell counts, pH values, sugar contents and colour parameters during storage were monitored. All strains exhibited better performances in PJ than in PSM, showing count values higher than 7 Log cfu g-1 up to 78 days of storage at 5°C. Almost all wild strains remained above the critical value of 6 Log cfu g-1 in samples stored at 25°C up to 45 days, while the Lb. rhamnosus GG type strain, used as control, was not able to survive later than 15 days. In the synthetic medium used the strains showed better survival in samples incubated at 25°C, remaining viable above the critical level up to 45 days of storage, except for the strain H12. The probiotic cultures added to jam did not significantly modify the colour parameters of the produce, however the metabolism of lactobacilli did determine changes in the pH and in the sugars composition
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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