348,324 research outputs found
RAM function is dependent on Kapβ2-mediated nuclear entry
No full textEukaryotic gene expression is dependent on the modification of the first transcribed nucleotide of pre-mRNA by the addition of the 7-methylguanosine cap. The cap protects transcripts from exonucleases and recruits complexes which mediate transcription elongation, processing and translation initiation. The cap is synthesised by a series of reactions which link 7-methylguanosine to the first transcribed nucleotide via a 5' to 5' triphosphate bridge. In mammals, cap synthesis is catalysed by the sequential action of RNGTT (RNA guanylyltransferase and 5'-phosphatase) and RNMT (RNA guanine-7 methyltransferase), enzymes recruited to RNA pol II during the early stages of transcription. We recently discovered that the mammalian cap methyltransferase is a heterodimer consisting of RNMT and the RNMT-activating subunit, RAM (RNMT-activating mini-protein). RAM activates and stabilises RNMT and thus is critical for cellular cap methylation and cell viability. Here we report that RNMT interacts with the N-terminal 45 amino acids of RAM, a domain necessary and sufficient for maximal RNMT activation. In contrast, smaller components of this RAM domain are sufficient to stabilise RNMT. RAM functions in the nucleus and we report that nuclear import of RAM is dependent on PY nuclear localisation signals and Kapß2 nuclear transport protein
Numerical simulation of ram extrusion in short-fiber-reinforced fresh cementitious composites
No full textThis is the author's accepted manuscript. The final published article is available from the link below. First published in JoMMS in 4(10), 2009, published by Mathematical Sciences Publishers.A series of ram extrusion tests was carried out on a short-fiber-reinforced, semisolid, fresh cementitious composite. An elastoviscoplastic constitutive model is proposed for the extrudable fresh cementitious composite. It features the associative flow rule, a nonlinear strain rate-hardening law, and the von Mises yield criterion. The model is then implemented in ANSYS/LS-DYNA explicit finite element code.
Various ram extrusion processes of the fresh cementitious composite were simulated. It has been found that the extrusion load versus imposed displacement predictions agree well with the experimental results. The fresh paste flow, through the die entry and the die-land, is then interpreted in light of the evolution of the deformation and distribution of state variables, mainly based on numerical results and the ram extrusion mechanism.
The effects of extrusion ratio and extrusion velocity on extrusion load are also investigated, based on the mechanical properties of the fresh cementitious composite. The study indicates that the numerical procedure established, together with the constitutive model proposed, is applicable for describing ram extrusion of short-fiber-reinforced fresh cementitious composites, which might provide a numerical rheometric tool from which ram extrusion of elastoviscoplastic paste-like materials can be examined and quantified.Hong Kong Research Grant Council and China Ministry of Science and Technology
Scientometric portrait of Ram Gopal Rastogi
No full textPublication productivity of Indian scientist (R.G. Rastogi) has been documented.
Scientometric analysis of 312 papers by Ram Gopal Rastogi published during 1954 to 1992 in various domains: (a) Luni -solar activity and quiet -time E & F- region (57); (b) Equatorial electric field and low and mid latitude iof:osphere (78); (c) Ionospheric E- region irregularities (19); (dj Ionospheric F- region irregularities (32); and (e) Magnetic disturbance effects on the equatorial low and mid latitude ionosphere (23) were analysed. Interdomainery contents and of the number of papers: a+b were 36; b+c and b+d were 20 each; b+e were 16;. c+e were 5; a+e were 3; d+e were 2; and a+d had only one publication. Highest collaborations were with H. Chandra (61), M.R. Deshpande (42), and G. Sethia (19) out of his total 97 collaborators. His highest productivity was during 1978 with 28 papers followed by 19 papers during 1977. The core journals preferred by him for publishing papers were: Indian Journal of Radio & Space Physics, India, and Journal of Atomic & Terrestrial Physics, UK (59 each), followed by Proceedings of the Indian Academy of Sciences, India (34). Most prolific title keywords with their frequencies were: Ionosphere (92); Equatorial (61); F-region (53); Equatorial electrojet region (40), and Magnetic equator (30)
Grand Rounds: Research Reproducibility - Ramkiran (Ram) Gouripeddi (12-05-2017)
No full textSpencer S. Eccles Health Sciences Library Grand Rounds: Research Reproducibility (GRRR). "Reproducible Informatics for Reproducible Research" presented by Ramkiran Gouripeddi, MS, MBBS, Research Assistant Professor, Biomedical Informatics. Dr. Ram Gouripeddi earned his MS from Arizona State University (2009) and his medical degree from MGR Medical University, India. He has broad interests in clinical research informatics. He participates in research in which investigators attempt to understand the requirements of the clinical research community and develop the means and tools to enable, accelerate and scale clinical research. GRRR is a weekly endeavor to raise awareness about reproducibility issues, showcase Utah researchers\u27 work, and to create an open forum for discussions. Dr. Ram Gouripeddi: https://medicine.utah.edu/faculty/mddetail.php?facultyID=u0741833 Grand Rounds: Research Reproducibility website: http://campusguides.lib.utah.edu/UtahRR18/GRRR Tweets about #UtahRR18 OR #MakeResearchTru
CHARACTERISTIC OF FROZEN-THAWED EPIDIDYMAL SPERMATOZOA AND REFRIGERATED STORAGE OF RAM SPERMATOZOA
No full textPost-mortem spermatozoa recovery is an important technique for obtaining germplasm reserves
from genetically valuable animals or endangered species. The purpose of this study was to study
whether ram spermatozoa within epididymides stored at 4º C for 24 and 48 h remain their motility and
viability. The characteristic of ram epididymal spermatozoa after freezing and thawing was also
observed. Six pairs of ram testes with attached epididymides were used in this study. The motility of
control spermatozoa was well maintained throughout the dilution procedure (83.3±1.1, 80±1.3, and
80±1.3% for collection, Niwa and Sasaki freezing -1 extender (NSF-1 and NSF-2 groups respectively);
but declined (P<0.05) after freezing and thawing (38.3±3.1%). Motile and viable spermatozoa could be
recovered from epididymides up to 48 h of storage, although their quality declined significantly
(P<0.05) as post-mortem storage time increased (motility: 83±1.2, 67±3.0, and 46±5.1; viability:
84.2±2.4, 73±2.8, and 66.6±2.6 % for control, 24 h and 48 h group respectively). These data indicate
that ram epididymides could be stored at 4º C for 48 h when epididymal spermatozoa cannot be
immmediately collected and cryopreserved. These storage conditions might be possible to use for
epididymal sperm recovery in wild ruminants.
Keywords: epididymal spermatozoa, Freezing, ram, refrigeratio
Scientometric Portrait of Nobel Laureate S. Chandrasekhar
No full textScientometric analysis of the publications productivity of Nobel Laureate S. Chandrasekhar is documented
A One Transistor RAM for MPC Projects
No full textMany MPC projects, such as video frame buffers, need a large memory subsystem. A one transistor per bit dynamic memory using Mead-Conway design rules is being designed with this purpose in mind. The memory cell size is 16.5 λ by 8λ (about the same size as a 1975 4K RAM cell with λ = 2.5 microns).
While a complete high density memory subsystem has not been designed, two chips have been designed to test its major components. One chip is a 1K memory array that tests the sense amplifier, column decoder/driver, and read/write logic. This chip lacks a timing generator and clock drivers. The second chip tests some low power bootstrapped clock drivers. These test chips are currently being fabricated
The relational model is dead‚ SQL is dead‚ and I don’t feel so good myself
No full textWe report the opinions expressed by well-known database researchers on the future of the relational model and SQL during a panel at the International Workshop on Non-Conventional Data Access (NoCoDa 2012), held in Florence, Italy in October 2012 in conjunction with the 31st International Conference on Conceptual Modeling. The panelists include: Paolo Atzeni (Università Roma Tre, Italy), Umeshwar Dayal (HP Labs, USA), Christian S. Jensen (Aarhus University, Denmark), and Sudha Ram (University of Arizona, USA). Quotations from movies are used as a playful though effective way to convey the dramatic changes that database technology and research are currently undergoing
Great Expectatrics: Great Papers, Great Journals, Great Econometrics
No full textThe paper discusses alternative Research Assessment Measures (RAM), with an emphasis on the Thomson Reuters ISI Web of Science database (hereafter ISI). The various ISI RAM that are calculated annually or updated daily are defined and analysed, including the classic 2-year impact factor (2YIF), 5-year impact factor (5YIF), Immediacy (or zero-year impact factor (0YIF)), Eigenfactor score, Article Influence, C3PO (Citation Performance Per Paper Online), h-index, Zinfluence, and PI-BETA (Papers Ignored - By Even The Authors). The ISI RAM data are analysed for 8 leading econometrics journals and 4 leading statistics journals. The application to econometrics can be used as a template for other areas in economics, for other scientific disciplines, and as a benchmark for newer journals in a range of disciplines. In addition to evaluating high quality research in leading econometrics journals, the paper also compares econometrics and statistics, alternative RAM, highlights the similarities and differences in alternative RAM criteria, finds that several ISI RAM capture similar performance characteristics for the leading econometrics and statistics journals while the new PI-BETA criterion is not highly correlated with any of the other ISI RAM, and hence conveys additional information regarding ISI RAM, highlights major research areas in leading journals in econometrics, and discusses some likely future uses of RAM.Research assessment measures, impact factors, Immediacy, Eigenfactor score, Article influence, h-index, C3PO, Zinfluence, PI-BETA
Great Expectatrics: Great Papers, Great Journals, Great Econometrics
No full textThe paper discusses alternative Research Assessment Measures (RAM), with an emphasis on the Thomson Reuters ISI Web of Science database (hereafter ISI). The various ISI RAM that are calculated annually or updated daily are defined and analysed, including the classic 2-year impact factor (2YIF), 5-year impact factor (5YIF), Immediacy (or zero-year impact factor (0YIF)), Eigenfactor score, Article Influence, C3PO (Citation Performance Per Paper Online), h-index, Zinfluence, and PI-BETA (Papers Ignored - By Even The Authors). The ISI RAM data are analysed for 8 leading econometrics journals and 4 leading statistics journals. The application to econometrics can be used as a template for other areas in economics, for other scientific disciplines, and as a benchmark for newer journals in a range of disciplines. In addition to evaluating high quality research in leading econometrics journals, the paper also compares econometrics and statistics, alternative RAM, highlights the similarities and differences in alternative RAM criteria, finds that several ISI RAM capture similar performance characteristics for the leading econometrics and statistics journals while the new PI-BETA criterion is not highly correlated with any of the other ISI RAM, and hence conveys additional information regarding ISI RAM, highlights major research areas in leading journals in econometrics, and discusses some likely future uses of RAM.Research assessment measures; impact factors; Immediacy; Eigenfactor score; Article influence; h-index; C3PO; Zinfluence; PI-BETA
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