530 research outputs found
Distribuição de temperatura em um escoamento de um fluído newtoniano entre duas placas porosas com gradiente de pressão pulsante
No full textDissertação (mestrado) - Universidade Estadual de Campinas. Instituto de Matemática, Estatística e Ciência da Computaçã
Development of composite calibration standard for quantitative NDE by ultrasound and thermography
No full textInspection of aircraft components for damage utilizing ultrasonic Non-Destructive Evaluation (NDE) is a time intensive endeavor. Additional time spent during aircraft inspections translates to added cost to the company performing them, and as such, reducing this expenditure is of great importance. There is also great variance in the calibration samples from one entity to another due to a lack of a common calibration set. By characterizing damage types, we can condense the required calibration sets and reduce the time required to perform calibration while also providing procedures for the fabrication of these standard sets. We present here our effort to fabricate composite samples with known defects and quantify the size and location of defects, such as delaminations, and impact damage. Ultrasonic and Thermographic images are digitally enhanced to accurately measure the damage size. Ultrasonic NDE is compared with thermography.This proceeding may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing.
This proceeding appeared in Dayal, Vinay, Zach G. Benedict, Nishtha Bhatnagar, and Adam G. Harper. "Development of composite calibration standard for quantitative NDE by ultrasound and thermography." In AIP Conference Proceedings, vol. 1949, no. 1, p. 060006. AIP Publishing LLC, 2018, and may be found at
DOI: 10.1063/1.5031552.
Copyright 2018 The Author(s).
Posted with permission
OS3: A Step Forward Towards Enhancing Share and Reuse in Serverless Functions
No full textKnown for its execution flexibility and pricing elasticity, Serverless computing deploys the code of services on demand and charges the client for their actual execution. Serverless computing enables service developers to focus on creating useful services, without being concerned about how these services would be deployed and provisioned. These developments have fostered the rapid growth of a community of open-source developers creating and improving various serverless functionalities. Because of this variety, the ecosystem of serverless functionalities suffers from a high degree of duplication, with multiple serverless functionalities sharing similarities or being outright identical. In order to integrate a serverless function into a project, a developer first needs to be able to find a suitable implementation among multiple alternatives. Unfortunately, existing search technologies have not been designed to address the needs of searching for serverless functionalities. To address this problem, this paper presents a novel approach to search for serverless functions, called Open-Source Serverless Search (OS3) that maximizes the utility of the returned serverless functions by (1) basing the search process on both descriptive keywords and library usages, thus increasing the search results' precision and completeness; (2) filtering and ranking the search results on both the software license and execution cost parameters. Implemented in 3,000 lines of Python, with a search space of 5,981 serverless repositories from four major serverless platforms, OS3 outperforms existing search approaches in terms of the suitability of the returned serverless functions, based on our evaluation with realistic use cases. Enhancing the search facilities for serverless functions with the insights presented herein can help fully fulfill the enormous promise of serverless computing.Master of Science (MS)Computer and Information Science, College of Engineering & Computer ScienceUniversity of Michigan-Dearbornhttp://deepblue.lib.umich.edu/bitstream/2027.42/176343/1/Sarvesh Bhatnagar Final Thesis.pd
Universal Statistical Properties of Inertial-particle Trajectories in Three-dimensional, Homogeneous, Isotropic, Fluid Turbulence
No full textWe obtain new universal statistical properties of heavy-particle trajectories in three-dimensional, statistically steady, homogeneous, and isotropic turbulent flows by direct numerical simulations. We show that the probability distribution functions (PDFs) P(Φ), of the angle Φ between the Eulerian velocity u and the particle velocity v, at a point and time, scales as P(Φ) ∼Φ−, with a new universal exponent ≃ 4
Rabies DNA vaccine encoding lysosome-targeted glycoprotein supplemented with Emulsigen-D confers complete protection in preexposure and postexposure studies in BALB/c mice
No full textThe worldwide incidence of rabies and the inability of currently used vaccination strategies to provide highly potent and cost-effective therapy indicate the need for an improved rabies vaccine. Thus, DNA vaccine based on lysosome-targeted glycoprotein of the rabies virus was evaluated in BALB/c mice. It imparted partial protection (60%) against challenge with 20 LD50 of the challenge virus standard (CVS) strain of rabies virus. To improve the outcome of vaccination, to ultimately enhance the immune response, we investigated different routes for DNA vaccine delivery, varied doses of DNA, and the influence of adjuvant supplementation. The highest immune response pertaining to IgG antibody titer, with a predominantly IgG1/IgG2a subclass distribution, effective cellular immunity, and a high level of rabies virus neutralizing antibodies (RVNAs) was attained by the optimized DNA vaccine formulation comprising intramuscular administration of 100 µg of DNA vaccine supplemented with Emulsigen®-D. In preexposure prophylaxis, a 3-dose regimen of this formulation generated a high RVNA titer (32 IU/ml) and conferred complete protection against challenge with 20 LD50 of CVS. For postexposure efficacy analysis, rabies was experimentally induced with 50 LD50 of CVS. Subsequent therapy with 5 doses of the formulation completely prevented rabies in BALB/c mice, which maintained protective RVNA titers of 4 IU/ml. The World Health Organization recommended rabies protective titer threshold is 0.5 IU/ml. Thus, this optimized DNA vaccine formulation provides an avenue for preventing and controlling rabies.-Kaur, M., Saxena, A., Rai, A., and Bhatnagar, R. Rabies DNA vaccine encoding lysosome-targeted glycoprotein supplemented with Emulsigen-D confers complete protection in preexposure and postexposure studies in BALB/c mice
A niosome formulation modulates the Th1/Th2 bias immune response in mice and also provides protection against anthrax spore challenge
No full textHimanshu Gogoi, Rajesh Mani, Rakesh Bhatnagar Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India Introduction: In this study, we have investigated the immunogenicity and protective efficacy of a niosomal formulation encapsulating protective antigen (PA) and PA domain 4 (D4) of Bacillus anthracis. Methods: Nonionic surfactant–based vesicles (NISV) + PA and NISV + D4 were prepared from span-60 and cholesterol by reverse-phase evaporation method and were evaluated for in vitro characteristics and immunological studies. Results: Particle characterization using transmission electron microscopy and atomic force microscopy analysis showed that the niosomal formulation was spherical in shape. The entrapment efficiency values were calculated to be 58.5% and 44.75% for PA and D4, respectively. Confocal microscopy and flow cytometry studies showed an enhanced uptake of antigen in THP1 macrophages by niosome as compared to antigen only. An in vitro release assay showed a burst release of antigen from niosome within 24 hours followed by a gradual release for 144 hours. Immunological studies showed that both PA- and D4-encapsulated niosome elicited a robust IgG titer. Antibody isotyping and cytokine profile showed that NISV + PA and NISV + D4 enhanced both Th1 and Th2 responses in mice, suggesting a mixed Th1/Th2 response. Both NISV + PA and NISV + D4 elicited high levels of anti-inflammatory cytokine interleukin-10 with low levels of pro-inflammatory cytokine tumor necrosis factor-α, suggesting the anti-inflammatory property of niosome. Both the niosomal formulations were also able to confer protection against BA infection as compared to only PA and D4. Conclusion: PA and D4 encapsulated NISV formulation could modulate both the Th1 and Th2 adaptive immune system and was found to be a better prophylactic against anthrax. Keywords: Bacillus anthracis, niosome, protective antigen, protective antigen domain
Performance and hardware complexity analysis of programmable radio platform for MIMO OFDM based WLAN systems
No full textEmerging wireless technologies and standards present a design space with multiple dimensions in terms of time, physical hardware space, and technology trends. Efficient evaluation of a desired combination of these dimensions to support multiple technologies and standards presents a significant challenge. We study the feasibility of a multiprotocol architecture without sacrificing the Quality of Service. An architecture facilitating such a mechanism can be implemented at different layers in the network stack with each layer offering a tradeoff between complexity and latency. Careful analysis of the physical layer reveals that most blocks of the transceiver can be reused for different protocols without significant architectural change. In addition to the feasibility analysis, we also identify common blocks in the network stack that could be possibly reused buying us significant hardware gains without sacrificing the aggregate system throughput. Our study presents the gate count complexity and the performance analysis of programmable radio architecture with the 802.11n (Draft 3.0) MIMO-OFDM based protocol stream and 802.11a OFDM based WLAN protocol stream. In this thesis, we demonstrate that multiple protocols can be supported using the same hardware under acceptable latency requirements. Complexity of the system in terms of gate count has been determined. It has been found that for shorter frame sizes, it is better to process less number of OFDM symbols at a time. However, for larger frame sizes, it is beneficial to process large number (four to eight) of OFDM symbols at a time. Also, the minimum clock rate required to run the hardware, would vary depending upon the number of OFDM Symbols processed. The switching and multiplexing overhead of the programmable radio platform has also been investigated. Finally, our simulator is capable of evaluating bottlenecks, if any.M.S.Includes bibliographical references (p. 75-76)
Novel application of trimethyl chitosan as an adjuvant in vaccine delivery
No full textAnshu Malik,* Manish Gupta,* Vatika Gupta, Himanshu Gogoi, Rakesh Bhatnagar Molecular Biology and Genetic Engineering Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India *These authors contributed equally to this work Abstract: The application of natural carbohydrate polysaccharides for antigen delivery and its adjuvanation potential has garnered interest in the scientific community in the recent years. These biomaterials are considered favorable candidates for adjuvant development due to their desirable properties like enormous bioavailability, non-toxicity, biodegradability, stability, affordability, and immunostimulating ability. Chitosan is the one such extensively studied natural polymer which has been appreciated for its excellent applications in pharmaceuticals. Trimethyl chitosan (TMC), a derivative of chitosan, possesses these properties. In addition it has the properties of high aqueous solubility, high charge density, mucoadhesive, permeation enhancing (ability to cross tight junction), and stability over a range of ionic conditions which makes the spectrum of its applicability much broader. It has also been seen to perform analogously to alum, complete Freund’s adjuvant, incomplete Freund’s adjuvant, and cyclic guanosine monophosphate adjuvanation, which justifies its role as a potent adjuvant. Although many review articles detailing the applications of chitosan in vaccine delivery are available, a comprehensive review of the applications of TMC as an adjuvant is not available to date. This article provides a comprehensive overview of structural and chemical properties of TMC which affect its adjuvant characteristics; the efficacy of various delivery routes for TMC antigen combination; and the recent advances in the elucidation of its mechanism of action. Keywords: trimethyl chitosan, chitosan, vaccine delivery, adjuvant, polyme
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