6 research outputs found
Separation of Radiocopper 64/67Cu from the Matrix of Neutron-Irradiated Natural Zinc Applicable for 64Cu Production
No full textRadioisotope 64Cu is a promising radiometallic-isotope for molecular-targeted-radiopharmaceuticals. Having a half-life of 12.70 hours and emitting β+-radiation (E+ = 0.6531 MeV) as well as β—ray (E = 0.5787 MeV), it is widely used in the form of biomedical-substrate-radiopharmaceutical for positron emission tomography (PET) diagnosis and simultaneously for targeted radiotherapy of cancer. The potential needs on the availability of 64Cu-labeled pharmaceuticals for domestic nuclear medicine hospitals lead to a necessity for the local production of carrier-free 64Cu using BATAN’s G.A. Siwabessy reactor because of the technical and economical constraints in the production using BATAN’s cyclotron. The presented work is accordingly to study whether the radioisotope 64Cu can be produced and separated from the matrix of post-neutron-irradiated-natural zinc. This study is expected can be further improved and implemented in production technology of carrier-free 64Cu based on 64Zn (n,p) 64Cu nuclear reaction exploiting the fast neutron fraction among the major thermal fraction due to unavailability of fast-neutron-irradiation facility in the BATAN’s G.A. Siwabessy reactor. The solution of post-neutron-irradiated-natural zinc in 1M acetic acid was loaded into Chelex-100 cation exchanger resin column to pass out the Zn/Zn* fraction whereas the Cu* fraction which remained in the column was then eluted out from the column by using 1.5 M HCl and loaded into the second column containing Dowex-1X8 anion exchanger resin. The second column was then eluted with 0.5 M HCl. The collected eluate was expected to be zinc-free Cu* fraction. It was observed from the half-life and the -spectrometric analysis that radioactive copper-64Cu containing 67Cu was produced by neutron activation on the natural Zn-foil target and can be separated from the target matrix by the presented two-steps-column-chromatographic separation technique. The radioactivity measurement showed that wrapping the Zn target with cadmium foil increased the activity of radioactive copper and, thus, the Cu*/Zn*-rati
SINTESIS DAN KARAKTERISASI NUKLEOTIDA BERTANDA [α-32P]ATP
No full textThe utilization of nuclear technology in health sector with molecular techniques is increasingly developed today, especially in Indonesia. One of which is nucleotide compound marked with [α-32P]ATP, this compound has been used as tracer for deoxyribonucleic acid (DNA)/ ribonucleic acid (RNA) in the study of various physiological and pathological processes. [α-32P]ATP is synthesized through several stages of continuous reaction in one reaction vessel. It begins with synthesis of [γ-32P]ATP through an enzymatic reaction, using H332PO4 and ADP, and enzymes of lactate dehydrogenase, 3-phosphoglycerate phosphokinase and glyceraldehide 3-phospho dehydrogenase; followed by phosphorilation of 3’AMP with T4 polinucleotide kinase enzyme to produce 3’-[5’-32P]ATP. The result is hydrolyzed with nuclease P1 enzyme to produce [5’-32P]AMP. The unreacted [γ-32P] is degraded by the addition of hexokinase enzyme and glucose. At the final stage of the reaction, the [5’-32P]AMP is phosphorilated using phosphoenol-piruvat, piruvat kinase, and myokinase to produce [α-32P]ATP. The test results show that the every stage of reaction is characterized using TLC method, PEI cellulose paper as stationary phase and KH3PO4 0,5 M pH 3,5 as mobile phase. At the end of reaction, the yield of [α-32P]ATP reaches 71,7%, at Rf = 0,2
Studi Awal Pembuatan Koloid Kromik Fosfat Bertanda Radioisotop 32P Sebagai Bahan Pembuatan Skin Patch
No full textKeloids are skin disorders or benign tumors that are due to abnormal wound healing in the binding tissue after a trauma, inflammation, surgical wounds, or burns. Low activity radioisotopes have shown to be effective in curing or eliminating keloids on the skin. One of these radioisotopes is phosphorus-32 (32P), a beta (β-) emitter with a half-life of 14.3 days. This radioisotope can also be developed for the treatment of keloid and skin tumors. Currently, keloid is treated by a conventional method e.g. by applying the bulk of 32P radioisotope directly on keloid area and this method is considered inefficient and less secure. The purpose of this research is to obtain technology for preparing of a 32P-labeled skin patch. The first step of this research is to produce 32P-labeled chromic phosphate (Cr32PO4) colloids, through condensation involving oxidation-reduction reaction. In this step, Cr (VI) is reduced to Cr (III) to form Cr32PO4 with a particle size of <1 μm. These particles (Cr32PO4) are to expect to distribute evenly when mixed with silicon to form skin patch which will not decompose easily. Characterization of the prepared Cr32PO4 colloids gave a yield of 97,8%. Geometric standard deviation (sg) of colloidal particles amounted to 163.7 nm shaped poly-disperse. Further study needs to be performed in due time in order to have Cr32PO4 colloids with suitable particle size
Studi Awal Pembuatan Koloid Kromik Fosfat Bertanda Radioisotop 32P Sebagai Bahan Pembuatan Skin Patch
No full textKeloids are skin disorders or benign tumors that are due to abnormal wound healing in the binding tissue after a trauma, inflammation, surgical wounds, or burns. Low activity radioisotopes have shown to be effective in curing or eliminating keloids on the skin. One of these radioisotopes is phosphorus-32 (32P), a beta (β-) emitter with a half-life of 14.3 days. This radioisotope can also be developed for the treatment of keloid and skin tumors. Currently, keloid is treated by a conventional method e.g. by applying the bulk of 32P radioisotope directly on keloid area and this method is considered inefficient and less secure. The purpose of this research is to obtain technology for preparing of a 32P-labeled skin patch. The first step of this research is to produce 32P-labeled chromic phosphate (Cr32PO4) colloids, through condensation involving oxidation-reduction reaction. In this step, Cr (VI) is reduced to Cr (III) to form Cr32PO4 with a particle size of <1 μm. These particles (Cr32PO4) are to expect to distribute evenly when mixed with silicon to form skin patch which will not decompose easily. Characterization of the prepared Cr32PO4 colloids gave a yield of 97,8%. Geometric standard deviation (sg) of colloidal particles amounted to 163.7 nm shaped poly-disperse. Further study needs to be performed in due time in order to have Cr32PO4 colloids with suitable particle size
Commissioning Test of a New Production Facility for Radioisotope Phosphorus-32 (32P) : Scale-up and Resin Analysis using Dry Distillation Separation Method: Pengujian Fasilitas Baru Untuk Produksi Radioisotop Fosfor-32 (32P) : Peningkatan Skala Produksi dan Analisis Resin Menggunakan Metode Pemisahan Distilasi Kering
No full textFasilitas produksi baru untuk radioisotop fosfor-32 (32P) harus memenuhi peraturan dan persyaratan yang berlaku. Komisioning pemenuhan peraturan dan persyaratan melibatkan tiga tahap yaitu pengujian sistem aliran udara, pengujian sistem distilasi kering, dan pengujian keamanan fasilitas. Tujuan dari penelitian ini adalah mengetahui kesiapan fasilitas produksi radioisotop 32P dengan parameter yang optimal, dan juga menganalisis tipe resin yang tepat dalam pemisahan / pemurnian produk 32P. Metode penelitian dilakukan dengan cara mengukur semua parameter kritis pada fasilitas proses produksi termasuk bagian keselamatan fasilitas, dan melakukan percobaan pengujian sistem distilasi kering serta pemisahanya menggunakan resin penukar ion. Berdasarkan hasil pengujian fasilitas produksi 32P menunjukan bahwa, parameter kristis dalam fasilitas tersebut memenuhi persayaratan minimum yaitu tekanan pada fasilitas glove box minimal -20 pascal dan laju aliran udara 0,50 m/s. Hasil pengukuran menunjukan bahwa fasilitas produksi 32P mencapai tekanan -50 Pascal dan laju aliran sirkulasi udara mencapai 1,80 s/d 2,40 m/s dengan sistem HVAC. Pengujian paparan radiasi sesuai ketentuan dan regulasi yang berlaku tidak boleh lebih dari 80 µSv/hari. Berdasarkan peengujian laju paparan radiasi mencapai 35 µSv/hari. Proses produksi 32P menggunakan sulfur alam dengan melakukan irradiasi Reaktor Nuklir G.A. Siwabessy dengan daya maksimum 15 MW. Hasil iradiasi sulfur kemudian diproses dengan cara distilasi kering untuk memishakan sulfur dan fosfor. Pemisahan radioisotope 32P dilakukan dengan dua jenis resin tipe AG50-WX8 dari Bio-Rad dan Dowex Chemical. Total radioaktivitas yang diperoleh dengan menggunakan fasilitas proses baru adalah 706,21 mCi. Kemurnian radiokimia 32P sebelum menggunakan resin adalah 93,76% dan setelah penggunaan resin Dowex Chemical mencapai 95,95%, resin Bio-Rad mencapai 99,99%. Pengujian fasilitas baru untuk memproduksi radioisotop fosfor-32 (32P) harus memenuhi peraturan dan persyaratan yang berlaku. Uji coba komisioning melibatkan tiga tahap: pengujian sistem aliran udara, pengujian sistem distilasi kering, dan pengujian keamanan fasilitas. Fasilitas baru ini diharapkan dapat meningkatkan kapasitas produksi 32P untuk memenuhi permintaan domestik. Proses produksi menggunakan resin kation untuk memisahkan dan memurnikan 32P. Pemilihan resin yang tepat dapat meningkatkan kualitas produk 32P, memastikan bahwa produk tersebut memenuhi persyaratan kemurnian radionuklida dan radiokimia yang lebih besar dari 95%. Bahan baku untuk memproduksi radioisotop adalah sulfur (99,99%) yang diiradiasi di Reaktor Nuklir G.A. Siwabessy dengan daya maksimum 15 MW. Distilasi kering kemudian digunakan untuk memisahkan bahan target sulfur menjadi fosfor-32. Distilat yang dihasilkan menjalani pengujian kontrol kualitas untuk radionuklida dan radiokimia. Penggunan kedua jenis terdiri dari resin AG50-WX8 dari Bio-Rad dan Dowex Chemical yang dilewatkan oleh larutan bulk 32P. Pengaturan kontrol untuk suhu proses distilasi adalah antara 440°C dan 450°C, dan hasil pengukuran pada titik no. 3 adalah 421°C dan 429°C. Total radioaktivitas yang diperoleh dengan target berat 10 gram belerang sebelum menggunakan fasilitas baru adalah 400 hingga 500 mCi, sedangkan setelah revitalisasi fasilitas diperoleh hasil 706,21 mCi. Penggunaan resin untuk meningkatkan kualitas produk 32P dengan membandingkan dua jenis resin. Sebelum menggunakan resin, kemurnian radiokimia adalah 93,76%. Namun, setelah menggunakan resin, kemurniannya meningkat, dengan resin Dowex Chemical mencapai 95,95% dan resin Bio-Rad mencapai 99,99%. Kemurnian radionuklida sebelum dan sesudah melewati resin adalah 99,99%. Fasilitas baru untuk produksi 32P telah beroperasi dengan baik dan memenuhi standar peraturan Badan Pengawas Tenaga Nuklir (BAPETEN) No. 16 tahun 2020 dan Peraturan Kepala Badan Pengawas Obat dan Makanan (BPOM) No. 23 tahun 2022. Resin yang optimal untuk digunakan dalam proses produksi adalah resin AG50-WX8 dari Bio-Rad, yang dapat meningkatkan kemurnian radiokimia hingga 99,99%
