9 research outputs found
Uji Enhancer Sebagai Penyebab Kesalahan Positif Pada Strip Uji Imunokromatografi Dengan Uji Banding Real-Time PCR.
No full textStrip uji imunokromatografi adalah salah satu metode deteksi babi pada
pangan. Metode ini mendeteksi babi secara cepat, mudah, dan murah, namun
memiliki kemungkinan terjadi kesalahan positif/ positif palsu. LPPOM MUI
menduga adanya kesalahan positif deteksi babi menggunakan strip uji pada bumbu
dari restoran yang bersertifikat halalMUI. Penelitian ini bertujuan mengidentifikasi
bahan yang menyebabkan kesalahan positif dan mengkaji penyebabnya. Pengujian
dilakukan pada sampel enhancer α, β, dan γ (bebas babi/halal), menggunakan strip
uji A, B, C, D, dan E. Sampel β terhadap strip uji A, B, dan E pada bobot 0.5 g
menunjukkan hasil positif ditandai dengan adanya garis merah pada test line dan
control line, seolah-olah mengandung babi. Sampel α dan γ terhadap strip uji A
pada bobot masing-masing 1.0 g dan 0.75 g menunjukkan hasil positif ditandai
dengan adanya garis merah pada test line dan control line, seolah-olah mengandung
babi. Uji banding real-time PCR menunjukkan bahwa tidak terdeteksi DNA babi
pada sampel α, β, dan γ. Kesalahan positif disebabkan oleh antibodi yang tidak
spesifik pada beberapa strip uji sehingga menyebabkan pengikatan terhadap antigen
non-target dan sifat asam amino X yang bermuatan sehingga menyebabkan
terbentuknya ikatan elektrostatik mengikuti model induce-fit
EKSPRESI DAN REGULASI GEN: TEORI DAN APLIKASI
No full textPenulis: Febrial Hikmah, S.Si., M.Biomed.
Burhannuddin, S.Si., M.Biomed.
Silviatun Nihayah, S.Si., M.Biomed.
Gladies Mercya Grameinie, S.Si., M.Biomed.
Luthfiana Hardianingtyas, S.Si., M.Biomed.
dr. Steven, M.Biomed.
Raafqi Ranasasmita, M.Biomed.
Gita Wideani, S.Si., M.Biomed.
Miftahuz Zakiyah, LC., S.Pd., M.Biomed.
Kinasih Prayuni, M.Si.
dr. Agil Wahyu Wicaksono, M.Biomed.
Nur Imaniati Sumantri, S.Si., M.Biotek.
ISBN: 978-623-89118-7-5
Tebal: x + 222 hlm, 23 x 15,5 cm
Juli 2024
Editor : Febrial Hikmah, S.Si., M.Biomed.
Penata Letak : Fiana Astika
Penata Sampul : Alwi Viandika
Penerbit:
PT. ADIKARYA PRATAMA GLOBALINDO
Dusun Tegalsari, RT 001/RW 004, Desa Jumoyo, Kec. Salam
Kabupaten Magelang, Provinsi Jawa Tengah
HP/WA: 08989999951, Email: [email protected]
Website: www.adpraglobalindo.my.id
ANGGOTA IKAPI
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Dilarang memperbanyak isi buku ini dalam bentuk dan dengan cara apapun tanpa izin tertulis dari penerbit
Ekstraksi DNA dari Daging Segar untuk Analisis dengan Metode Loop-Mediated Isothermal Amplification (LAMP)
Get PDFDNA extraction is needed in the analysis using the Loop-mediated isothermal amplification (LAMP) method because this method identifies nucleic acids. Some extraction methods that can be selected including commercial kits extraction method and phenol-chloroform extraction method. The purpose of this study was to obtain the best quality DNA extract between the two extraction methods. The DNA extraction process produced DNA concentrations between 31.06 - 410.18 ng / ml for the commercial kit DNA extract and 212.60 - 1502.30 ng / ml for the phenol-choroform DNA extract, while the purity of DNA were 1.82-2.02 for commercial kit DNA extract and 1.93-2.02 for phenol-chloroform DNA extract. The concentration and purity of extracts produced from both methods meet the requirements for molecular analysis. The purity and visualization results of commercial kit DNA extract are better than those produced from extraction from the phenol-chloroform method. DNA extract obtained from the commercial kit method was chosen to be used in the amplification stage of the method (LAMP)
The Influence of DMBA (7,12-dimethylbenz-[a]anthracene) Regimen In The Development of Mammae Carcinogénesis on Sprague Dawley Female Rat
No full textThere are many methods for development of mammae cancer animal model, one of which is chemical induction using carcinogenic agent, DMBA. This research aimed to explore the influence of dose and time regimens of DMBA on development of mammae carcinogenesis on Sprague dawley female rats. The first study was 50 rats treated with 20 mg/kg bw of DMBA orally for eleven times at twice a week. Morphological evaluations were conducted with mammae palpation for 15 weeks and then all of rats were sacrificed for collecting mammae organs for histological analysis using hematoxylin-eosin staining. The results showed that the first and the latest nodules appeared at the fourth-week and the fourteenth-week after ending DMBA induction, respectively, in which the most often nodule appearances were at the seventh-week. The number of nodule incidence and multiplicity were by 74% and 2 noduls/rat, respectively. Histological analysis of mammae glands determined that they fell under in Ductal Carcinoma Invasive (DCIV) category. The second study was 25 rats gavaged orally with DMBA at dose 20 mg/kg bw for five times every three days. After palpating for 15 weeks, the results showed that no nodule was observed but the histological analysis demonstrated developing of mammae gland carcinogenesis reaching about 60% Ductal Carcinoma Insitu (DCIS) and 40% Ductal Carcinoma Invasive (DCIV) stages. Based on the results of this study can be concluded that the dose and frequency of DMBA will affect the successful development of mammary gland carcinogenesis. In DMBA induction with low frequency, no data showed the incidence and multiplicity of tumor, but histopathologic level carcinogenesis can be distinguished. In DMBA induction with high frequency, incidence and multiplicity of tumor data can be obtained but can not be distinguished histopathologically.Keywords: DMBA, doses, carcinogenesis, mammae, histopatology</jats:p
Correlation between hypoxia inducible factor -1α and renin expression in rats kidney induced by cobalt chloride
Get PDFBackground: Cobalt chloride can be used as an agent to stabilize hypoxia inducible factor-1α (HIF-1α) and to imitate hypoxia without low levels of oxygen inside the body. We intended to investigate if there was any regulation of renin expression by HIF-1α. Therefore, we conducted several studies to clarify this possibility starting with the induction of hypoxic mimicry in rats by intra-peritoneal (IP) injection of cobalt chloride (CoCl2) to obtain the levels and pattern of HIF-1α and renin mRNA and protein expression.
Methods: Twenty-four rats were randomly divided into four groups, control group and incubation groups 2, 8, and 24 hours after intra-peritoneal injection of 30 mg CoCl2 per kg BW. After the rats were sacrificed, kidneys were excised, weighed and kidney weight compared to BW. Tissue parameters were measured such as RNA concentration, HIF-1α protein by ELISA, and renin mRNA by RT-PCR.
Results: Differences between the groups in the ratios of kidney weight to BW and in the concentrations of HIF-1α protein were statistically not significant (p > 0.05). Relative expression of renin mRNA increased markedly starting 8 hours after CoCl2 IP injection (30 times over controls) and further rising until 24 hours (2465 times over controls). Correlation between HIF-1α and renin mRNA by Pearson analysis was strongly positive, but not significant (R = 0.91; p = 0.09).
Conclusion: Renin gene regulation in renal hypoxic mimicry strongly correlates withHIF-1α. (Med J Indones. 2012;21:128-32
Antihypertensive Effect of Brucea javanica (L.) Merr. Fruit Extract
No full textEthnopharmacologically, the fruit of Brucea javanica (L.) Merr. is acknowledged in the Indonesian community to lower blood pressure. This study assessed the antihypertensive effect of B. javanica fruit extract using adrenaline-induced hypertensive Sprague Dawley rats. Hypertensive rats were divided into 4 groups: group A was given B. javanica water fraction and adrenaline, group B was given B. javanica hexane fraction and adrenaline, group C was given bisoprolol and adrenaline and group D was given adrenaline solely. Systolic blood pressure was regularly measured using the tail-cuff method. Treatment of adrenaline-induced hypertensive rats independently given B. javanica water fraction, the hexane fraction, and the bisoprolol group proved to significantly reduce blood pressure by 72.75 mmHg (-34%), 58.5 mmHg (-28%) and 23.25 mmHg (-12%) respectively, while there was an increase of 15.00 mmHg (+9%) SBP in the negative control group given solely adrenaline. The water fraction contains flavonoid and alkaloid. The hexane fraction of this fruit contains alkaloid. Our study suggest that the flavanoid and alkaloid content in B. javanica fruit work synergistically to alleviate hypertension, possibly through β1-adrenergic receptor-related mechanism
Study of the Modification of Loop-mediated Isothermal Amplification (LAMP) using Taq Polymerase for Halal Testing
No full textIntegration of ISO 22000 (2018) and HAS 23000 through Management System Audit: Case Study in Corned Beef Producer
Get PDFIntegrated Management System (IMS) based audit can assist the internal and external auditor to conduct an audit effectively and efficiently while checking compliance of Food Safety Management System and Halal Assurance System in the food industry. Corned beef is a product categorized as critical both in terms of halal and food safety. Implementing a food safety management system and halal assurance in corned beef industries is a challenge for producers and external auditors from inspection agencies. Based on the requirements equality approach, an Integrated Management System can be developed, referring to ISO 22000 : 2018 and HAS 23000. This research aims to combine the requirements of ISO 22000 : 2018 and HAS 23000 to be used for audit activity, as well as formulating recommendations for the corned beef producers in both requirements based on a new version of ISO 22000 : 2018. According to the analysis of requirements equality on each requirement objective, there are 14 of 30 sub-clauses of ISO 22000 : 2018 that can be integrated with 9 HAS criteria. There are 2 HAS criteria that cannot be integrated with sub clauses of ISO 22000 : 2018, namely criteria number 4 material and 5 product. When the IMS clauses are used in audit to one of corned beef company, the percentage of compliance with the IMS requirements is 90.6% in PT XYZ. The nonconformities related to ISO 22000 : 2018 found on a new clause that has not existed in the previous version ISO 22000: 2005. This gap creates some recommendations for PT XYZ. However, IMS based audit makes the audit for halal and food safety compliance be more effective that can combine checking of food safety and halal in one audit. Furthermore, IMS based audit makes the audit be more time efficient, by reducing the mandays
Antihypertensive Effect of Brucea javanica (L.) (Merr.) Fruit Extract
No full textEthnopharmacologically, the fruit of Brucea javanica (L.) Merr. is acknowledged in the Indonesian community to lower blood pressure. To prove such a claim, this paper assessed the antihypertensive effect of B. javanica fruit extract using adrenaline-induced hypertensive Sprague Dawley rats. Hypertensive rats were divided into 4 groups: group A was given B. javanica water fraction and adrenaline, group B was given B. javanica hexane fraction and adrenaline, group C was given bisoprolol and adrenaline and group D was given adrenaline solely. Systolic blood pressure was regularly measured using the tail-cuff method. Treatment of adrenaline-induced hypertensive rats independently given B. javanica water fraction, the hexane fraction, and the bisoprolol group proved to significantly reduce blood pressure by 72.75 mmHg (-34%), 58.5 mmHg (-28%) and 23.25 mmHg (-12%) respectively, while there was an increase of 15.00 mmHg (+9%) SBP in the negative control group given solely adrenaline. The water fraction contains flavonoid and alkaloid. The hexane fraction of this fruit contains alkaloid. Our study suggests that the flavanoid and alkaloid content in B. javanica fruit, work synergistically to alleviate hypertension, possibly through β1-adrenergic receptor-related mechanism.Keywords: adrenaline, antihypertensive, β1-adrenergic, Brucea javanica, hypertensio
