1,720,960 research outputs found
MOLECULAR ANALYSIS OF PBMSP-1 GENE IN ERYTHROCYTE OF MICE INFECTED WITH Plasmodium berghei
No full textThis study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp)
Morphology and Parasitaemia Development of Plasmodium berghei in Balb/c Mice (Mus musculus)
No full textMalaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Malaria disease caused by Plasmodium parasite have symptoms that typically include fever, fatigue, vomiting and headaches. In severe cases, it can cause yellow skin, seizures, coma or death. The present study is aimed to monitoring parasitemia level and percentage of parasite morphology as parasitaemia progresses. This research used Plasmodium berghei NK strain obtained from National University of Malaysia which originally from MR4, USA. Design used in this research was completely randomized design, with 2 treatments which were mice without infection and mice with infection of P. berghei parasite. Method used in this research was staining method of thin smear of blood using Giemsa stained and observed by microscopic. Parameter of observation were morphology and development of P. berghei at different level of parasitaemia (10%, 20%, 30% and 40%). The result showed from microscopic examination of blood slides prepared from the study animals indicated that the ring form was dominant stage obtained at all different stages of infection followed by trophozoite stage. Meanwhile schizont stage was the lowest stage obtained at all different stages observed
Bioactive Compound and Cytotoxic Analysis of Premna oblongifolia Merr. Ethanol Extract on Rat Bone Marrow-Derived MSC-like Cells
Get PDFThe use of natural products in regenerative medicine holds significant promise, especially in regions where traditional medicine is prevalent. This study investigates the bioactivity of the ethanol extract of green cincau leaves (Premna oblongifolia Merr.) (GCE) on bone marrow-derived mesenchymal stem cell-like cells (BM-MSCs). The leaves were extracted using the maceration method, and the bioactive compounds were analysed and detected by Gas Chromatography-Mass Spectroscopy (GC-MS). The cytotoxicity tests were conducted across a concentration range of 1–1200 μg/mL using WST-1 reagent, and cell viability was measured at 24, 48, and 72 h. The results of GC-MS indicated 28 bioactive compounds with the dominant groups being terpenoids and fatty acids. The most abundant compounds in GCE were phytol (19.06%), squalene (4.32%), Urs-12-en-28-oic acid, 3-hydroxy-, methyl ester, (3.beta.)- (6.23%) and 8,11,14-Eicosatrienoic acid, (Z,Z,Z)- (2.88%). The GCE maintained cell viability above 80% at all concentrations, indicating low cytotoxicity. Furthermore, Alizarin Red S staining revealed calcium deposit formation at 300 µg/mL, suggesting preliminary osteogenic activity. These findings suggest GCE may have potential for supporting BM-MSCs proliferation and demonstrates early osteogenic properties. Further investigation is recommended to explore its mechanism of action and therapeutic potential in differentiation applications
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination extender of lactose and glycerol
No full textEriani K, Sari N, Ihdina M, Rosnizar. 2017. The effect of equilibration time on semen freezing of local swamp buffalo (Bubalus bubalis) with combination extender of lactose and glycerol. Nusantara Bioscience 9: 77-82. This study was to determine the different time of equilibration on semen of swamp buffalo (Bubalus bubalis) which was diluted by adding Tris-Egg Yolk extender of lactose and glycerol cryoprotectant combination. Fresh sperm of the swamp buffalo (B. bubalis) was diluted by using Tris-Egg Yolk extender of lactose cryoprotectants combination 0 mM (L0), 60 mM (L60), 120 mM (L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 2,5, 3, and 4 hours. The parameter used in this study was the percentage of sperm motility and live treatment. The results of the 2.5-hour equilibration with a combination of cryoprotectants L60G7 showed the best quality in all parameters. The results of the 3-hour equilibration with combination of cryoprotectants L60G7 showed the best quality in all parameters whereas the best quality in all parameters in 4-hour equilibration was the combination of cryoprotectants L120G7.</jats:p
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