1,720,957 research outputs found
ANALYSIS OF BIOCHEMICAL PROPERTIES RELATED TO HARD BUNCH PHENOMENA IN OIL PALM (Elaeis guineensis Jacq.)
No full textOPTIMASI PRIMER SINGLE NUCLEOTIDE AMPLIFIED POLYMORPHYSM (SNAP) PADA GEN BRASSINOSTEROID (BRI) KELAPA SAWIT
Get PDFOptimisation of Single Nucleotide Amplified Polymorphism (SNAP) Primers in Brassinosteroid (BRI) Gene of Oil Palm Oil palm varieties that have high yields still have a high rate of stem height increase, and it has an impact on difficulties at harvest. Brassinosteroid (BRI) is one of the growth hormones that regulate stem height increase. In this study, the sample used is three types of oil palm, namely E. guineensis which represents the height growth of normal oil palm stems, E. oleifera which represents slow stem height growth, and the result of crossing or hybrid Eo x Eg which is expected to inherit the traits between the two the crossed parent. Sequencing was carried out on samples using a specific primer for the BRI gene that triggers elongation and division of stem cells. The Single Nucleotide Amplified Polymorphisms (SNAP) primers used in the analysis process need to find the optimum temperature to obtain the optimum PCR conditions. Four primers of Single Nucleotide Amplified Polymorphism (SNAP) that get the optimum temperature with a mixture of normal reagents are BRI 69 Alt-Rev and BRI 562 Ref-Rev at 55oC, BRI 1206 Alt-Rev at 57oC and BRI 1206 Ref-Rev at 58oC. Two primers require additional MgCl2 in the reactant mixture, namely BRI 69 Ref-Rev at 55oC and BRI 2115 Alt-Rev at 56oC.Keywords: Oil palm, Brassinosteroid, Primer, DNA, GenesABSTRAK Varietas-varietas kelapa sawit yang memiliki daya hasil tinggi umumnya masih memiliki laju pertambahan tinggi yang relatif cepat dan berakibat pada kesulitan saat panen. Brassinosteroid (BRI) merupakan salah satu hormon pertumbuhan yang mengatur pertambahan tinggi batang. Pada studi ini, Sampel yang digunakan adalah tiga jenis kelapa sawit yaitu sampel E. guineensis yang mewakili pertumbuhan tinggi batang kelapa sawit normal, E. oleifera yang mewakili pertumbuhan tinggi batang lambat, dan hasil persilangan atau hibrida Eo x Eg yang diharapkan mewarisi sifat antara kedua induk yang disilangkan. Sekuensing dilakukan pada sampel menggunakan primer spesifik gen BRI yang memicu pemanjangan dan pembelahan sel batang. Primer Single Nucleotide Amplified Polymorphisms (SNAP) yang digunakan dalam proses analisis perlu dicari suhu optimumnya agar didapatkan kondisi PCR yang optimum sehingga dihasilkan produk PCR yang spesifik. Empat primer Single Nucleotide Amplified Polymorphism (SNAP) yang mendapatkan suhu optimum dengan campuran pereaksi normal yaitu BRI 69 Alt-Rev dan BRI 562 Ref-Rev pada suhu 55oC, BRI 1206 Alt-Rev pada suhu 57oC dan BRI 1206 Ref-Rev pada suhu 58oC. Dua primer membutuhkan tambahan MgCl2 pada campuran pereaksinya yaitu BRI 69 Ref-Rev pada suhu 55oC dan BRI 2115 Alt-Rev pada suhu 56oC.Kata Kunci: Kelapa sawit, Brassinosteroid, Primer, DNA, Ge
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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