1,721,057 research outputs found
Erratum: Identification of the goat CSN1S1F allele by means of PCR-RFLP method (Animal Genetics (2000) 31 (342))
No full textErratum
Ramunno L., Cosenza G., Pappalardo M., Pastore N., Gallo D., Di Gregorio P. & Masina P. (2000) Identi®cation of
the goat CSN1S1F allele by means of PCR-RFLP method. Animal Genetics 31, 34
Genetic variability at the FASN locus in the Italian Mediterranean river buffalo (bubalus bubalis, 2n=50).
No full textFatty acid synthase (FASN) is a enzyme complex that plays a central
role in de novo synthesis of long-chain satured fatty acids
(SFA). The structural and functional characterization of the FASN gene was reported in several species and genetic diversity
has been found in cattle. For instance, the SNP g.763G>C has
been associated with milk fat content in dairy cattle, whereas
other SNPs have been associated with the FAs composition of
adipose and milk fat. Despite these information in cattle, no
studies have been carried out in a closely related ruminant, the
river buffalo. In Italy this species is important in economic
terms, being connected to the production of Mozzarella PDO.
Although the buffalo genome project indicated the FASN
sequence (EMBL ID AWWX01575345.1), this is not annotated
and no data on genetic diversity are available so far. Therefore,
the aim of this study was to explore the natural genetic variability
existing at FASN. Genomic DNA was isolated from 17 individual
blood samples collected in different farms of Campania
region. A preliminary investigation was carried out by PCR on
the region spanning the exons 6-9 by using the following primers
(For: 5’-CTGACCAAGAAGTCCCTG-3’ and Rev: 5’-AAGGAGTTGATGCCCACG-
3’) designed on the homologous bovine gene (EMBL
ID AF285607.2). PCR products were analysed in 1.5% TBE
agarose gel, purified and sequenced. Sequence analysis was performed
by DNAsis software. A DNA fragment of 1345 bp including
the exons 6-9 of the buffalo FASN gene was amplified and
sequenced. The analysis of the sequences showed 7 SNPs. Five
polymorphisms were found at intronic level, whereas 2 SNPs
were exonic and both responsible of amino acid change. In particular,
the SNP g.5976A>C at the 99th nt of the exon 6 is responsible
for a Asn256>His replacement, whereas the SNP
g.6100A>T at the 15th nt of the exon 7 is responsible for the
change Thr265>Ser (numbering is relative to the homologous
gene in cattle). These SNPs are the first polymorphisms reported
at FASN locus for the Italian river buffalo. Considering the influence
of the FASN on the FA composition and given that the average
fat content in buffalo milk was 8.16% in 2013, the observed
variability is interesting because it offers potential tools for the
set up of future association studies with fat traits and for the
application of marker assisted selection programmes to improve
the productivity of this species
Studio sui caprini autoctoni del Molise. IV. Alcuni fattori influenzanti la galattopoiesi
No full textSu 81 capre allevate in provincia di Isernia (Molise), distintamente per ordine di lattazione (2.- 8. e oltre), è stato studiato l'andamento della curva di lattazione mediante il metodo proposto da Wood ed è stata calcolata la quantità di latte prodotto a 90, 120, 150 e 180 giorni dal parto.
I valori medi di produzione riferiti ai suddetti periodi sono risultati piú elevati in 4a lattazione con 87, 112, 131 e 145 kg e piú bassi in 7a lattazione con 65, 87, 105 e 118 kg, rispettivamente.
La funzione di Wood ha descritto in modo soddisfacente la variazione della quantità di latte prodotto in funzione della distanza dal parto.
Sono state osservate relazioni significative tra produzione lattea e alcune caratteristiche fanerotiche, quantificabili in differenze di produzione, in 180 giorni di lattazione, varianti dall'8 al 23 per cento
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Individual dairy curd production and association analysis with 14 candidate genes in Italian buffalo
No full textThe genetic merit of Italian buffaloes is mainly related to theability of producing mozzarella cheese. This trait is measured by the estimated mozzarella production (PK M index) according to Altiero et al. (1989). Several studies have shown that PKM based only on milk yield, fat (FP) and protein percentage (PP) may over-or under- estimate the real mozzarella yield. The individual curd production (ICP) is proposed as index of the mozzarella making ability. Values were compared with estimated PKM. Associations between ICP and 14 loci related to milk traits (ACACA, BLG, CSN1S1, CSN1S2, CSN2, CSN3, LEP, LPL, LTF, OXT, OXTR, PRL, PRLRand SCD) were tested. Idividual milk samples of 233 Italian river buffaloes were collected monthly along the whole lactation. A total o f36 variable swere measured: Fat(FP),protein(PP) and lactose % (LP), milk coagulation properties (r,clottingtime;k20,curd firming time; a30, curd firmness at 30min), SH, pH, Individual Curd Production at 24h (ICP24), etc. Cows were genotyped for published and new SNPs found in the explored genes. Associations between phenotypes and candidate gene polymorphisms were tested using a mixed linear model.
Two out of 16 markers in 2 out of 14 investigated genes gave significant associations with ICP24 and FP: OXTR (g.129C>T) and SCD (g.-461A>C). The genotype AC at SCD showed an over-dominance effect with a higher increase vs AA of 13.15 g/kg milk (p=0.015) for ICP24 and + 0.54 FP (p=0.010). The estimated PKM/ICP ratio showed that the PKM under-estimates the prediction of mozzarella yield ( on average 0.929).These data confirm the findings of Parlato and Zicarelli (2015) on the necessity of a new prediction model, and offer useful indication for the application of MAS programs in buffalo
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